公开(公告)号：WO2011142836A3

公开(公告)日：2012-07-05

申请号：PCT/US2011000887

申请日：2011-05-16

Applicant: FLUIDIGM CORP ,  JONES ROBERT C ,  LIVAK KENNETH J ,  MAY ANDREW ,  MIR ALAIN ,  PIEPRZYK MARTIN ,  QIN JIAN ,  RAMAKRISHNAN RAMESH ,  SPURGEON SANDRA ,  WANG JUN ,  ZIMMERMANN BERNHARD G

Inventor： JONES ROBERT C ,  LIVAK KENNETH J ,  MAY ANDREW ,  MIR ALAIN ,  PIEPRZYK MARTIN ,  QIN JIAN ,  RAMAKRISHNAN RAMESH ,  SPURGEON SANDRA ,  WANG JUN ,  ZIMMERMANN BERNHARD G

IPC: C12Q1/68 ,  G01N33/52 ,  G06F19/18

CPC classification number: C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6858 ,  G01N33/542 ,  G01N33/582 ,  G01N33/6893 ,  G01N2800/368 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/155 ,  C12Q2535/131 ,  C12Q2537/143 ,  C12Q2537/16 ,  C12Q2563/173

Abstract: The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.

Abstract translation: 本发明提供用于检测基因型，突变和/或非整倍体的基于扩增的方法。 这些方法具有广泛的适用性，但是特别适合于检测和定量存在于母体体液样品中的游离胎儿DNA中的靶核酸。

公开(公告)号：WO2011142836A9

公开(公告)日：2012-01-05

申请号：PCT/US2011000887

申请日：2011-05-16

Applicant: FLUIDIGM CORP ,  JONES ROBERT C ,  LIVAK KENNETH J ,  MAY ANDREW ,  MIR ALAIN ,  PIEPRZYK MARTIN ,  QIN JIAN ,  RAMAKRISHNAN RAMESH ,  SPURGEON SANDRA ,  WANG JUN ,  ZIMMERMANN BERNHARD G

Inventor： JONES ROBERT C ,  LIVAK KENNETH J ,  MAY ANDREW ,  MIR ALAIN ,  PIEPRZYK MARTIN ,  QIN JIAN ,  RAMAKRISHNAN RAMESH ,  SPURGEON SANDRA ,  WANG JUN ,  ZIMMERMANN BERNHARD G

IPC: C12Q1/68 ,  G01N33/52 ,  G06F19/18

CPC classification number: C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6858 ,  G01N33/542 ,  G01N33/582 ,  G01N33/6893 ,  G01N2800/368 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/155 ,  C12Q2535/131 ,  C12Q2537/143 ,  C12Q2537/16 ,  C12Q2563/173

Abstract: The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.

Abstract translation: 本发明提供了用于检测基因型，突变和/或非整倍性的基于扩增的方法。 这些方法具有广泛的适用性，但特别适合于检测和定量存在于母体液样品中的游离胎儿DNA中的靶核酸。

公开(公告)号：EP2414547A4

公开(公告)日：2012-08-22

申请号：EP10759511

申请日：2010-04-02

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA ,  ANDERSON MEGAN

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2527/143 ,  C12Q2535/122 ,  C12Q2549/119 ,  C12Q2563/179 ,  C12Q2565/629

公开(公告)号：EP2707507A4

公开(公告)日：2015-03-18

申请号：EP12782232

申请日：2012-05-09

Applicant: FLUIDIGM CORP

Inventor： LIVAK KENNETH J ,  MYERS STACEY ,  WANG XIAOHUI ,  WANG JUN

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q2525/151

公开(公告)号：EP2569447A4

公开(公告)日：2013-11-27

申请号：EP11780930

申请日：2011-05-16

Applicant: FLUIDIGM CORP ,  PIEPRZYK MARTIN

Inventor： PIEPRZYK MARTIN ,  JONES ROBERT C ,  LIVAK KENNETH J ,  MAY ANDREW ,  MIR ALAIN ,  QIN JIAN ,  RAMAKRISHNAN RAMESH ,  SPURGEON SANDRA ,  WANG JUN ,  ZIMMERMANN BERNHARD G

IPC: C12Q1/68 ,  G01N33/52 ,  G06F19/18

CPC classification number: C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6858 ,  G01N33/542 ,  G01N33/582 ,  G01N33/6893 ,  G01N2800/368 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/155 ,  C12Q2535/131 ,  C12Q2537/143 ,  C12Q2537/16 ,  C12Q2563/173

公开(公告)号：CA2757560C

公开(公告)日：2018-11-13

申请号：CA2757560

申请日：2010-04-02

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA ,  ANDERSON MEGAN

IPC: C12Q1/68 ,  B81B7/04 ,  C12M1/34 ,  C12M1/36 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/686 ,  C40B20/04 ,  C40B30/04 ,  C40B70/00 ,  G01N33/50

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

公开(公告)号：EA023190B1

公开(公告)日：2016-05-31

申请号：EA201171206

申请日：2010-04-02

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA ,  ANDERSON MEGAN

IPC: C12Q1/68

Abstract: Вопределенныхвариантахосуществленияданноеизобретениеобеспечиваетспособыамплификации, вкоторыхнуклеотиднуюметку(и) и, факультативно, штрих-кодовуюнуклеотиднуюпоследовательностьдобавляютк целевымнуклеотиднымпоследовательностям. Вдругихвариантахосуществленияданноеизобретениеобеспечиваетмикрофлюидноеустройство, котороевключаетмножествопервыхлинийвводаи множествовторыхлинийввода. Микрофлюидноеустройствотакжевключаетмножествонаборовпервыхкамери множествонабороввторыхкамер. Каждыйнаборпервыхкамернаходитсяв жидкостнойсвязис однимизмножествапервыхлинийввода. Каждыйнаборвторыхкамернаходитсяв жидкостнойсвязис однимизмножествавторыхлинийввода. Микрофлюидноеустройстводополнительновключаетмножествопервыхплунжерныхпарв жидкостнойсвязис первойчастьюмножествавторыхлинийвводаи множествовторыхплунжерныхпарв жидкостнойсвязис второйчастьюмножествавторыхлинийввода.

公开(公告)号：AU2010232439B2

公开(公告)日：2015-07-16

申请号：AU2010232439

申请日：2010-04-02

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA ,  ANDERSON MEGAN

IPC: C12Q1/68

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

公开(公告)号：SG194722A1

公开(公告)日：2013-12-30

申请号：SG2013080858

申请日：2012-05-09

Applicant: FLUIDIGM CORP

Inventor： LIVAK KENNETH J ,  MYERS STACEY ,  WANG XIAOHUI ,  WANG JUN

公开(公告)号：HK1170543A1

公开(公告)日：2013-03-01

申请号：HK12111058

申请日：2012-11-02

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA ,  ANDERSON MEGAN

IPC: C12Q20060101

Abstract: The present invention provides an elastomer microfluidic device that enables simultaneous amplification of multiple nucleic acid samples with multiple pairs of primers in a matrix format as well as recovery of the products e.g. for next generation sequencing. The flow of samples and reagents as well as the compartmentalization of individual reactions in the multiple channels and chambers is controlled using valves and dilation pumping / volumetric capacitive pumping.