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    MICROFLUIDIC DEVICES WITH REMOVABLE COVER AND METHODS OF FABRICATION AND APPLICATION
    3.
    发明申请
    MICROFLUIDIC DEVICES WITH REMOVABLE COVER AND METHODS OF FABRICATION AND APPLICATION 审中-公开
    具有可移除覆盖层的微流体器件以及制造和应用方法

    公开(公告)号：WO2011040884A3

    公开(公告)日：2011-06-03

    申请号：PCT/SG2010000369

    申请日：2010-09-29

    Applicant: FLUIDIGM CORP ,  COHEN DAVID ,  MAY ANDREW ,  PIEPRZYK MARTIN ,  FOWLER BRIAN ,  LEE KIM HUAT ,  YAN JUN ,  ZHOU MING FANG ,  NG SENG BENG

    Inventor： COHEN DAVID ,  MAY ANDREW ,  PIEPRZYK MARTIN ,  FOWLER BRIAN ,  LEE KIM HUAT ,  YAN JUN ,  ZHOU MING FANG ,  NG SENG BENG

    IPC: G01N35/08 ,  G01N33/48

    CPC classification number: B01L3/5027 ,  B01L3/502738 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/12 ,  B01L2300/045 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0655 ,  G01N1/405

    Abstract: The present invention includes microfluidic systems having a microfabricated cavity that may be covered with a removable cover, where the removable cover allows at least part of the opening of the microfabricated cavity to be exposed or directly accessed by an operator. The microfluidic systems comprise chambers, flow and control channels formed in elastomeric layers that may comprise PDMS. The removable cover comprises a thermoplastic base film bonded to an elastomer layer by an adhesive layer. When the removable cover is peeled off, the chamber is at least partially open to allow sample extraction from the chamber. The chamber may have macromolecular crystals formed inside or resulting contents from a PCR reaction. The invention also includes a method for making vias in elastomeric layers by using the removable cover. The invention further includes methods and devices for peeling the peelable cover or a removable component such as Integrated Heater Spreader.

    Abstract translation: 本发明包括具有可用可移除覆盖物覆盖的微制造腔体的微流体系统，其中可移除覆盖物允许微制造腔体的至少部分开口被操作员暴露或直接存取。 微流体系统包括腔室，形成在可包含PDMS的弹性体层中的流动和控制通道。 可移除的盖子包括通过粘合层粘合到弹性体层的热塑基膜。 当可移除盖子被剥离时，腔室至少部分打开以允许从腔室抽取样品。 室内可能形成大分子晶体或由PCR反应产生内容物。 本发明还包括一种通过使用可移除盖子来制造弹性体层中的通孔的方法。 本发明进一步包括用于剥离可剥离盖或诸如集成式加热器分散器的可移除部件的方法和装置。

    NUCLEIC ACID ENCODING REACTIONS
    4.
    发明申请
    NUCLEIC ACID ENCODING REACTIONS 审中-公开
    核酸编码反应

    公开(公告)号：WO2012162267A3

    公开(公告)日：2014-05-15

    申请号：PCT/US2012038894

    申请日：2012-05-21

    Applicant: FLUIDIGM CORP ,  ANDERSON MEGAN ,  CHEN PEILIN ,  FOWLER BRIAN ,  JONES ROBERT C ,  KAPER FIONA ,  LEBOFSKY RONALD ,  MAY ANDREW

    Inventor： ANDERSON MEGAN ,  CHEN PEILIN ,  FOWLER BRIAN ,  JONES ROBERT C ,  KAPER FIONA ,  LEBOFSKY RONALD ,  MAY ANDREW

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12Q1/6855 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/65 ,  C12N15/66 ,  C12Q1/686 ,  C12Q1/6874

    Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

    Abstract translation: 本文所描述的方法可用于掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列，一个或多个靶核苷酸序列。 在具体实施方案中，产生具有适配器（例如适合用于高通量DNA测序）的核酸片段。 在其它实施方案中，关于反应混合物的信息被编码成反应产物。 本文还描述了可用于扩增一种或多种靶核酸以准备应用如双向核酸测序的方法和试剂盒。 在具体实施方案中，本发明的方法还需要进行双向DNA测序。 本文还描述了通过引物延伸来编码和检测和/或定量等位基因的方法。

    METHOD AND SYSTEM FOR CRYSTALLIZATION AND X-RAY DIFFRACTION SCREENING
    6.
    发明申请
    METHOD AND SYSTEM FOR CRYSTALLIZATION AND X-RAY DIFFRACTION SCREENING 审中-公开
    用于结晶和X射线衍射筛选的方法和系统

    公开(公告)号：WO2008141183A2

    公开(公告)日：2008-11-20

    申请号：PCT/US2008063247

    申请日：2008-05-09

    Applicant: FLUIDIGM CORP ,  FOWLER BRIAN ,  MAY ANDREW

    Inventor： FOWLER BRIAN ,  MAY ANDREW

    IPC: G01T1/16

    CPC classification number: G01N23/20025

    Abstract: An integrated fluidic circuit includes a substrate layer and a first structure coupled to the substrate layer and including a plurality of channels. The first structure is configured to provide for flow of one or more materials through the plurality of channels. The integrated fluidic circuit also includes a second structure coupled to the substrate layer. The second structure includes a plurality of control channels configured to receive an actuation pressure. The integrated fluidic circuit is characterized by a thickness of less than 1.5 mm.

    Abstract translation: 集成流体回路包括衬底层和耦合到衬底层并且包括多个通道的第一结构。 第一结构被配置为提供通过多个通道的一种或多种材料的流动。 集成流体回路还包括耦合到衬底层的第二结构。 第二结构包括被配置为接收致动压力的多个控制通道。 集成流体回路的特征在于厚度小于1.5mm。

    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS
    7.
    发明申请
    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS 审中-公开
    颗粒群体的单粒子分析

    公开(公告)号：WO2013177206A3

    公开(公告)日：2014-01-30

    申请号：PCT/US2013042086

    申请日：2013-05-21

    Applicant: FLUIDIGM CORP ,  ANDERSON MEGAN ,  CHEN PEILIN ,  FOWLER BRIAN ,  KAPER FIONA ,  LEBOFSKY RONALD ,  MAY ANDREW

    Inventor： ANDERSON MEGAN ,  CHEN PEILIN ,  FOWLER BRIAN ,  KAPER FIONA ,  LEBOFSKY RONALD ,  MAY ANDREW

    IPC: C12Q1/68

    CPC classification number: C12Q1/6813 ,  B01D15/08 ,  C12Q1/6806 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out "in parallel," i.e., essentially simultaneously in the separate reaction volumes.

    Abstract translation: 在某些实施方案中，本发明提供了用于测定颗粒群体中的单个颗粒的方法和装置，其中针对每个颗粒测量至少两个参数。 当颗粒处于分开的反应体积中时，可以测量一个或多个参数。 或者或另外，可以在稍后的分析步骤中测量一个或多个参数，例如其中在分开的反应体积中进行反应并回收和分析反应产物。 在具体实施方案中，一个或多个参数测量“并行地进行”，即基本上同时在单独的反应体积中进行。

    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS
    10.
    发明公开
    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS 有权
    方法，系统和设备采集和处理几个单独的细胞的微流控芯片

    公开(公告)号：EP2820394A4

    公开(公告)日：2015-07-08

    申请号：EP13754093

    申请日：2013-02-28

    Applicant: FLUIDIGM CORP

    Inventor： FOWLER BRIAN ,  KIMBALL JAKE ,  MAUNG MYO THU ,  MAY ANDREW ,  NORRIS MICHAEL C ,  TOPPANI DOMINIQUE G ,  UNGER MARC A ,  WANG JING ,  WEST JASON A A

    IPC: G01N15/10

    CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

    Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

    Abstract translation: 方法，系统和装置被描述为多个单细胞捕获和处理利用微流体。 提供了用于捕获，分区和/或个体细胞从细胞中产生地连同遗传信息和/或与每个单独的电池反应的更大的人口操纵的工具和技术。 不同的捕获配置可以被用于捕获个人的细胞，然后在处理的多室反应配置每个单独的小区。 一些实施例可以提供特定目标扩增，全基因组扩增，全转录扩增，实时PCR制备，拷贝数变异，预扩增，测序mRNA和/或多个单独的电池单元型thathave是来自于较大的人口划分 细胞。 一些实施例可以提供用于其它应用。 一些实施例可以被配置为单个细胞或相关联的反应产物进行成像作为处理的一部分。 反应产物可以收获和/或在某些情况下，进一步的分析。

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