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公开(公告)号：EP0670373A4
公开(公告)日：1999-08-18
申请号：EP93923652
申请日：1993-10-25
Applicant: FUSO PHARMACEUTICAL IND , OHNO TSUNEYA
Inventor： OHNO TSUNEYA , HIROTSU TAKUO , KESHI HIROYUKI , MATSUHISA AKIO
CPC classification number: C12Q1/6895
Abstract: The invention provides a probe useful for rapidly detecting and identifying fungi causative of infectious diseases by digesting completely a DNA extracted from a fungus Candidat albicans with EcoRI, inserting the same into an appropriate cloning vector, and picking up a probe containing DNA fragments unique to each fungus. The invention also provides a standard sequence suitable for making reference to a genomic DNA contained in a clinical specimen by elucidating the base sequence of the probe thus picked up.
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公开(公告)号：EP0785277A4
公开(公告)日：1997-09-10
申请号：EP95933618
申请日：1995-10-02
Applicant: FUSO PHARMACEUTICAL IND
Inventor： KESHI HIROYUKI , EDA SOJI , UEHARA HIROTSUGU , UEYAMA HIROSHI , MATSUHISA AKIO
CPC classification number: C12Q1/689
Abstract: A probe derived from phlogogenic bacteria of pneumonia, containing fragments of DNA which streptococcus pneumoniae essentially possesses, and useful for detecting and identifying causative bacteria of pneumonia is obtained by completely digesting the DNA with a restriction endonuclease Pst I, followed by cloning into a suitable vector.
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公开(公告)号：JPH10304896A
公开(公告)日：1998-11-17
申请号：JP6583698
申请日：1998-03-16
Applicant: FUSO PHARMACEUTICAL IND , ONO NORIYA
Inventor： ONO NORIYA , MATSUHISA AKIO , UEHARA KEIJI , EDA SOJI
IPC: C12N15/09 , C12N15/11 , C12Q1/68 , C12R1/01 , C12R1/19 , C12R1/22 , C12R1/385 , G01N33/53 , G01N33/566
Abstract: PROBLEM TO BE SOLVED: To obtain the subject new probe having a base sequence contained in a genomic DNA of Pseudomonas aeruginosa, comprising a DNA specifically being reacted with the DNA of the bacterium, useful for diagnosing infections with the bacterium. SOLUTION: This probe for diagnosing infections is useful for diagnosing infections with Pseudomonas aeruginosa and is specifically reacted with a DNA which Pseudomonas aeruginosa possesses. The new probe for diagnosing infections comprises at least one base sequence among base sequences of formula I, formula II, etc., contained in a genomic DNA of Pseudomonas aeruginosa. The probe is obtained by digesting the genomic DNA of Pseudomonas aeruginosa separated from a patient infected with the bacterium with HindIV and selecting a fragment crossed by hybridization between a plasmid into which the fragment of the digestion is inserted and a chromosome DNA derived from the bacterium.
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公开(公告)号：JPH10304895A
公开(公告)日：1998-11-17
申请号：JP6582098
申请日：1998-03-16
Applicant: FUSO PHARMACEUTICAL IND , ONO NORIYA
Inventor： ONO NORIYA , MATSUHISA AKIO , UEHARA KEIJI , EDA SOJI
IPC: C12N15/09 , C12N15/11 , C12Q1/68 , C12R1/01 , C12R1/19 , C12R1/22 , C12R1/385 , G01N33/53 , G01N33/566
Abstract: PROBLEM TO BE SOLVED: To obtain the subject new probe having a base sequence contained in a genomic DNA of Enterococcus faecalis, comprising a DNA specifically being reacted with the DNA of the bacterium, useful for diagnosing infections with the bacterium. SOLUTION: This probe for diagnosing infections is useful for diagnosing infections with Enterococcus faecalis and is specifically reacted with a DNA which Enterococcus faecalis possesses. The new probe for diagnosing infections comprises at least one base sequence among base sequences of formula I, formula II, etc., contained in a genomic DNA of Enterococcus faecalis. The probe is obtained by separating the genomic DNA of Enterococcus faecalis separated from a patient infected with the bacterium, digesting the DNA with HindIV and selecting a fragment crossed by hybridization between a plasmid into which the fragment of the digestion is inserted and a chromosome DNA derived from the bacterium.
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公开(公告)号：JPH10304897A
公开(公告)日：1998-11-17
申请号：JP6585098
申请日：1998-03-16
Applicant: FUSO PHARMACEUTICAL IND , ONO NORIYA
Inventor： ONO NORIYA , MATSUHISA AKIO , UEHARA KEIJI , EDA SOJI
IPC: C12N15/09 , C12N15/11 , C12Q1/68 , C12R1/01 , C12R1/19 , C12R1/22 , C12R1/385 , G01N33/53 , G01N33/566
Abstract: PROBLEM TO BE SOLVED: To obtain the subject new probe being specifically reacted with DNAs which Escherichia coli, Klebsiella pneumoniae and Enterobacter cloacae possess, comprising a DNA contained in a genomic DNA of Escherichia coli. SOLUTION: This probe for diagnosing infections is useful for diagnosing infections with infectious disease-causing bacteria and is specifically reacted with DNAs which Escherichia coli, Klebsiella pneumoniae and Enterobacter cloacae possess. The new probe for diagnosing infections comprises at least one base sequence among base sequences of formula I, formula II, etc., contained in a genomic DNA of Escherichia coli. The probe is obtained by digesting the genomic DNA of the bacteria separated from a patient infected with the bacteria and or their combination with HindIV and selecting a fragment crossed by hybridization between a plasmid into which the fragment of the digestion is inserted and a chromosome DNA derived from the bacteria.
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Patent Agency Ranking
公开(公告)号：JPH10262676A
公开(公告)日：1998-10-06
申请号：JP7107997
申请日：1997-03-25
Applicant: FUSO PHARMACEUTICAL IND
Inventor： KAMIYAMA HIROSHI , FUKUDA KANAKO , KARASHI HIROYUKI , MATSUHISA AKIO
Abstract: PROBLEM TO BE SOLVED: To obtain the subject probe containing a DNA fragment produced by the restriction enzyme treatment of a DNA sequence of a specific microorganism and useful for the detection and identification of causal microorganisms of endoabdominal infection, infection of female genital organs, sepsis, etc. SOLUTION: The subject microorganism is cultured under anaerobic condition, the DNA extracted from the cultured product is completely digested with HindIII and randomly cloned with vector pGEM-3Z and a probe containing a DNA sequence exhibiting specific reaction with the DNA of the subject microorganism is selected. The reactivity of each probe with the DNA of a clonic microbial strain is confirmed by dot blot hybridization and a DNA probe confirmed to have species specificity (e.g. a probe containing the base sequence of the formula) is collected. The causal microorganism of infectious diseases is quickly and accurately identified by this probe without culturing or proliferating the microorganism.
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公开(公告)号：JPH0889295A
公开(公告)日：1996-04-09
申请号：JP23634894
申请日：1994-09-30
Applicant: FUSO PHARMACEUTICAL IND
Inventor： KARASHI HIROYUKI , EDA SOJI , UEHARA KEIJI , KAMIYAMA HIROSHI , MATSUHISA AKIO
Abstract: PURPOSE: To obtain a probe for diagnosing infectious diseases, containing a specific DNA fragment, specifically reacting DNA possessed by Streptococcus pneumoniae, capable of directly detecting an infectious disease cause microorganism taken in a phagocyte without multiplying the microorganism and rapidly and accurately identifying it. CONSTITUTION: This probe for diagnosing infectious diseases contains a DNA fragment obtained by treating a DNA possessed by Streptococcus pneumoniae, an infectious disease cause microorganism, with restriction enzyme PstI, is specifically reacted with the DNA possessed by Streptococcus pneumoniae and contains a base sequence of the formula.
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公开(公告)号：JPH06133798A
公开(公告)日：1994-05-17
申请号：JP28580292
申请日：1992-10-23
Applicant: FUSO PHARMACEUTICAL IND , ONO NORIYA
Inventor： HIROTSU TAKUO , KARASHI HIROYUKI , MATSUHISA AKIO , ONO NORIYA
Abstract: PURPOSE:To obtain a probe derived from a pathogenic fungus of an infectious disease and useful for detection and identification of a fungus. CONSTITUTION:A DNA of Candida albicans is extracted and the extracted DNA is thoroughly digested with EcoRI. Cloning is carried out using a suitable vector and a probe containing a DNA fragment peculiar to each fungus is selected. Further, the base sequence of the selected probe is determined. Thus, a fungus can be specifically detected and quickly identified. The determined base sequence is useful as a guideline for producing a primer for PCR and as a standard sequence suitable for comparison with a genomic DNA contained in a clinical sample.
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公开(公告)号：JPH1033179A
公开(公告)日：1998-02-10
申请号：JP19431796
申请日：1996-07-24
Applicant: FUSO PHARMACEUTICAL IND
Inventor： KARASHI HIROYUKI , EDA SOJI , UEHARA KEIJI , NISHIDA KEIGO , MATSUHISA AKIO
Abstract: PROBLEM TO BE SOLVED: To quickly and accurately diagnose the presence of Helicobacter pylori by diagnosing an alimentary disease using a DNA fragment specifically reacting with a DNA (or an RNA) contained in the Helicobacter pylori. SOLUTION: This probe is obtained by subjecting a chromosomal DNA contained in Helicobacter pylori to a hybridization with a DNA (e.g. having a base sequence of the formula) specifically reacting with a DNA contained in the Helicobacter pylori and containing a DNA fragment obtained by digesting the chromosomal DNA with a restriction enzyme Hind III. The diagnosis necessitates only about 1-2 days. The kind of a bacterium can rapidly and easily be diagnosed from the presence or absence of amplification of a DNA by a PCR method, when using a primer prepared by referring to the base sequence of the DNA specifically reacting with the DNA contained in the Helicobacter pylori.
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公开(公告)号：JPH047357B2
公开(公告)日：1992-02-10
申请号：JP3994684
申请日：1984-03-01
Applicant: FUSO PHARMACEUTICAL IND
Inventor： MATSUHISA AKIO , MORINAGA KUNIO , SAKAI HEIICHI
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