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    PROBE FOR DIAGNOSING INFECTION
    5.
    发明专利
    PROBE FOR DIAGNOSING INFECTION 失效

    公开(公告)号：JPH10304897A

    公开(公告)日：1998-11-17

    申请号：JP6585098

    申请日：1998-03-16

    Applicant: FUSO PHARMACEUTICAL IND ,  ONO NORIYA

    Inventor： ONO NORIYA ,  MATSUHISA AKIO ,  UEHARA KEIJI ,  EDA SOJI

    IPC: C12N15/09 ,  C12N15/11 ,  C12Q1/68 ,  C12R1/01 ,  C12R1/19 ,  C12R1/22 ,  C12R1/385 ,  G01N33/53 ,  G01N33/566

    Abstract: PROBLEM TO BE SOLVED: To obtain the subject new probe being specifically reacted with DNAs which Escherichia coli, Klebsiella pneumoniae and Enterobacter cloacae possess, comprising a DNA contained in a genomic DNA of Escherichia coli. SOLUTION: This probe for diagnosing infections is useful for diagnosing infections with infectious disease-causing bacteria and is specifically reacted with DNAs which Escherichia coli, Klebsiella pneumoniae and Enterobacter cloacae possess. The new probe for diagnosing infections comprises at least one base sequence among base sequences of formula I, formula II, etc., contained in a genomic DNA of Escherichia coli. The probe is obtained by digesting the genomic DNA of the bacteria separated from a patient infected with the bacteria and or their combination with HindIV and selecting a fragment crossed by hybridization between a plasmid into which the fragment of the digestion is inserted and a chromosome DNA derived from the bacteria.

    PROBE FOR DIAGNOSING INFECTIOUS DISEASE
    6.
    发明专利
    PROBE FOR DIAGNOSING INFECTIOUS DISEASE 失效

    公开(公告)号：JPH0889295A

    公开(公告)日：1996-04-09

    申请号：JP23634894

    申请日：1994-09-30

    Applicant: FUSO PHARMACEUTICAL IND

    Inventor： KARASHI HIROYUKI ,  EDA SOJI ,  UEHARA KEIJI ,  KAMIYAMA HIROSHI ,  MATSUHISA AKIO

    IPC: C12N15/09 ,  C07H21/04 ,  C12Q1/04 ,  C12Q1/68 ,  C12R1/46

    Abstract: PURPOSE: To obtain a probe for diagnosing infectious diseases, containing a specific DNA fragment, specifically reacting DNA possessed by Streptococcus pneumoniae, capable of directly detecting an infectious disease cause microorganism taken in a phagocyte without multiplying the microorganism and rapidly and accurately identifying it. CONSTITUTION: This probe for diagnosing infectious diseases contains a DNA fragment obtained by treating a DNA possessed by Streptococcus pneumoniae, an infectious disease cause microorganism, with restriction enzyme PstI, is specifically reacted with the DNA possessed by Streptococcus pneumoniae and contains a base sequence of the formula.

    PROBE FOR DIAGNOSING INFECTION
    7.
    发明专利
    PROBE FOR DIAGNOSING INFECTION 失效

    公开(公告)号：JPH10304896A

    公开(公告)日：1998-11-17

    申请号：JP6583698

    申请日：1998-03-16

    Applicant: FUSO PHARMACEUTICAL IND ,  ONO NORIYA

    Inventor： ONO NORIYA ,  MATSUHISA AKIO ,  UEHARA KEIJI ,  EDA SOJI

    IPC: C12N15/09 ,  C12N15/11 ,  C12Q1/68 ,  C12R1/01 ,  C12R1/19 ,  C12R1/22 ,  C12R1/385 ,  G01N33/53 ,  G01N33/566

    Abstract: PROBLEM TO BE SOLVED: To obtain the subject new probe having a base sequence contained in a genomic DNA of Pseudomonas aeruginosa, comprising a DNA specifically being reacted with the DNA of the bacterium, useful for diagnosing infections with the bacterium. SOLUTION: This probe for diagnosing infections is useful for diagnosing infections with Pseudomonas aeruginosa and is specifically reacted with a DNA which Pseudomonas aeruginosa possesses. The new probe for diagnosing infections comprises at least one base sequence among base sequences of formula I, formula II, etc., contained in a genomic DNA of Pseudomonas aeruginosa. The probe is obtained by digesting the genomic DNA of Pseudomonas aeruginosa separated from a patient infected with the bacterium with HindIV and selecting a fragment crossed by hybridization between a plasmid into which the fragment of the digestion is inserted and a chromosome DNA derived from the bacterium.

    PROBE FOR DIAGNOSING INFECTION
    8.
    发明专利
    PROBE FOR DIAGNOSING INFECTION 失效

    公开(公告)号：JPH10304895A

    公开(公告)日：1998-11-17

    申请号：JP6582098

    申请日：1998-03-16

    Applicant: FUSO PHARMACEUTICAL IND ,  ONO NORIYA

    Inventor： ONO NORIYA ,  MATSUHISA AKIO ,  UEHARA KEIJI ,  EDA SOJI

    IPC: C12N15/09 ,  C12N15/11 ,  C12Q1/68 ,  C12R1/01 ,  C12R1/19 ,  C12R1/22 ,  C12R1/385 ,  G01N33/53 ,  G01N33/566

    Abstract: PROBLEM TO BE SOLVED: To obtain the subject new probe having a base sequence contained in a genomic DNA of Enterococcus faecalis, comprising a DNA specifically being reacted with the DNA of the bacterium, useful for diagnosing infections with the bacterium. SOLUTION: This probe for diagnosing infections is useful for diagnosing infections with Enterococcus faecalis and is specifically reacted with a DNA which Enterococcus faecalis possesses. The new probe for diagnosing infections comprises at least one base sequence among base sequences of formula I, formula II, etc., contained in a genomic DNA of Enterococcus faecalis. The probe is obtained by separating the genomic DNA of Enterococcus faecalis separated from a patient infected with the bacterium, digesting the DNA with HindIV and selecting a fragment crossed by hybridization between a plasmid into which the fragment of the digestion is inserted and a chromosome DNA derived from the bacterium.

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