公开(公告)号：EP2740790A4

公开(公告)日：2015-01-21

申请号：EP12819778

申请日：2012-07-18

Applicant: FUSO PHARMACEUTICAL IND

Inventor： YAO TATSUMA ,  ASAYAMA YUTA ,  MATSUHISA AKIO

IPC: C12N5/073 ,  C08K5/00 ,  C08L101/00

CPC classification number: C12N5/0604 ,  C12N2500/32

公开(公告)号：EP0965646A4

公开(公告)日：2003-01-02

申请号：EP97932996

申请日：1997-07-24

Applicant: FUSO PHARMACEUTICAL IND

Inventor： KESHI HIROYUKI ,  EDA SOJI ,  UEHARA HIROTSUGU ,  NISHIDA KEIGO ,  MATSUHISA AKIO

IPC: C12N15/09 ,  C07H21/04 ,  C12Q1/04 ,  C12Q1/68 ,  C12R1/01

CPC classification number: C12Q1/689

Abstract: Probes for specifically detecting and identifying H. pylori which serves as an inflammatory bacterium in digestive diseases. These probes contain a fragment formed by completely digesting a DNA carried by H. pylori with a restriction enzyme HindIII. The information of the base sequences of these probes are useful as a structural indication of primers to be used in the construction of probes specific to H. pylori by PCR or as standard sequences suitable for the comparison with genomic DNAs contained in clinical specimens.

公开(公告)号：EP0974658A4

公开(公告)日：2002-10-09

申请号：EP98909856

申请日：1998-03-23

Applicant: FUSO PHARMACEUTICAL IND

Inventor： UEYAMA HIROSHI ,  ABE KANAKO ,  KESHI HIROYUKI ,  MATSUHISA AKIO

IPC: C12N15/09 ,  C07H21/04 ,  C07K14/315 ,  C12N15/00 ,  C12N15/31 ,  C12Q1/68 ,  C12R1/46

CPC classification number: C12Q1/689 ,  C07K14/315

Abstract: Probes obtained by extracting DNAs carried by Streptococcus pyogenes, completely digesting the extracted DNAs with a restriction enzyme HindIII, cloning the same into an appropriate vector and then screening probes containing the DNA fragments inherently carried by S. pyogenes, followed by the clarification of the base sequences of these probes.

公开(公告)号：EP1403369A4

公开(公告)日：2005-06-15

申请号：EP02778910

申请日：2002-05-27

Applicant: FUSO PHARMACEUTICAL IND

Inventor： OHNO TSUNEYA ,  MATSUHISA AKIO ,  KESHI HIROYUKI ,  ABE KANAKO ,  SUGIMOTO NORIHIKO ,  UEYAMA HIROSHI ,  EDA SOJI ,  UEHARA HIROTSUGU ,  IWAMI TAKAHISA ,  YAMAMOTO SEIJI ,  ARAKI HIROMASA

IPC: C12Q1/68 ,  G01N33/50 ,  C12N15/09 ,  C12N5/06 ,  C12N5/10 ,  G01N33/53

CPC classification number: G01N33/5047 ,  C12Q1/6841 ,  C12Q1/689 ,  G01N33/5005

Abstract: Phagocytes having taken a foreign microorganism which are prepared by bringing phagocytes into contact with the microorganism and then isolating the phagocytes; a process for producing the same; and a method and a kit with the use of the same. An experimental model whereby the phagocytosis function of phagocytes can be evaluated in vitro .

公开(公告)号：EP0725149A4

公开(公告)日：1999-08-18

申请号：EP95907490

申请日：1993-08-10

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  SHIBA KIYOTAKA ,  MIKAWA YOSHIKAZU ,  KISHI YUICHIRO

IPC: C12Q1/68 ,  G01N27/447 ,  G01N33/50 ,  G01N33/58 ,  C12Q1/28 ,  C12Q1/42

CPC classification number: C12Q1/6816 ,  C12Q1/6813

Abstract: A method of detecting a nucleic acid which comprises bringing a solid carrier suspected to carry or contain a nucleic acid into contact with a polyamine containing, bound thereto, a label capable of generating a detectable signal or a precursor thereof to thereby form a complex between a nucleic acid, if any, and the polyamine, removing the polyamine which has not formed any complex before or after the conversion of the precursor, if used, into the label, and detecting the label.

Abstract translation: 一种检测核酸的方法，其包括将怀疑携带或含有核酸的固体载体与含有与其结合的多胺接触能够产生可检测信号的标记或其前体的标记，从而在 核酸（如果有的话）和多胺，除去在前体转化之前或之后没有形成任何复合物的多胺（如果使用的话）到标签中，并检测标签。

公开(公告)号：EP0995800A4

公开(公告)日：2004-08-04

申请号：EP98909855

申请日：1998-03-23

Applicant: FUSO PHARMACEUTICAL IND

Inventor： UEYAMA HIROSHI ,  ABE KANAKO ,  KESHI HIROYUKI ,  MATSUHISA AKIO

IPC: C12N15/09 ,  C07H21/04 ,  C12N15/00 ,  C12N15/31 ,  C12Q1/68 ,  C12R1/01

CPC classification number: C12Q1/689

Abstract: Probes obtained by extracting DNAs carried by Bacteroides fragilis, completely digesting the extracted DNAs with a restriction enzyme HindIII, cloning the same into an appropriate vector and then screening probes containing the DNA fragments inherently carried by B. fragilis, followed by the clarification of the base sequences of these probes.

公开(公告)号：EP0652291A4

公开(公告)日：1999-08-18

申请号：EP93914968

申请日：1993-07-07

Applicant: FUSO PHARMACEUTICAL IND ,  OHNO TSUNEYA

Inventor： OHNO TSUNEYA ,  MATSUHISA AKIO ,  UEHARA HIROTSUGU ,  EDA SOJI

IPC: C12N15/09 ,  C12N15/11 ,  C12Q1/68 ,  C12R1/01 ,  C12R1/19 ,  C12R1/22 ,  C12R1/385 ,  G01N33/53 ,  G01N33/566

CPC classification number: C12Q1/689 ,  C12Q2600/156

Abstract: A probe useful for rapidly detecting and identifying bacteria causative of infectious diseases is provided by completely digesting DNAs extracted from principal bacteria causative of infectious diseases with Hind III, cloning the digestion product to a suitable vector, and selecting a probe containing DNA fragments peculiar to each of the bacteria. A standard DNA sequence suitable for comparison with that of a genomic DNA contained in a clinical specimen is provided by clarifying the base sequence of the selected probe.

公开(公告)号：EP1721991A4

公开(公告)日：2007-10-17

申请号：EP05709892

申请日：2005-02-08

Applicant: FUSO PHARMACEUTICAL IND

Inventor： MATSUHISA AKIO ,  YAMAMOTO SEIJI ,  EDA SOUJI ,  YAMASAKI SHINJI

IPC: C12Q1/68 ,  C12N15/10 ,  G01N33/50

CPC classification number: C12Q1/68 ,  C12Q1/6834 ,  C12Q1/6841 ,  C12Q2531/113

Abstract: It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detection kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then a nucleic acid thus amplified is detected. Since the nucleic acids are not extracted from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.

公开(公告)号：EP1403381A4

公开(公告)日：2005-01-12

申请号：EP02726495

申请日：2002-05-27

Applicant: FUSO PHARMACEUTICAL IND ,  OHNO TSUNEYA

Inventor： OHNO TSUNEYA ,  MATSUHISA AKIO

IPC: C12N15/31 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/566 ,  G01N33/569

CPC classification number: C12Q1/689 ,  C12Q1/04

Abstract: A microorganism causative of an infection is quickly and highly sensitively detected and/or identified by obtaining phagocytes from a clinical specimen containing phagocytes originating in a living body, immobilizing the obtained phagocytes, treating the phagocytes so as to enhance the permeability of the phagocyte membrane and expose DNA of the microorganism causative of the infection which is anticipated as being contained in the phagocytes, and then using a detection DNA probe hybridizable with the DNA under stringent conditions.

公开(公告)号：EP0972833A4

公开(公告)日：2002-10-09

申请号：EP98909854

申请日：1998-03-23

Applicant: FUSO PHARMACEUTICAL IND

Inventor： UEYAMA HIROSHI ,  ABE KANAKO ,  KESHI HIROYUKI ,  MATSUHISA AKIO

IPC: C12N15/09 ,  C07H21/04 ,  C12N15/00 ,  C12N15/31 ,  C12Q1/68 ,  C12R1/22 ,  C07K14/315

CPC classification number: C12Q1/689

Abstract: Probes obtained by extracting DNAs carried by Klebsiella pneumoniae, completely digesting the extracted DNAs with a restriction enzyme HindIII, cloning the same into an appropriate vector and then screening probes containing the DNA fragments inherently carried by K. pneumoniae, followed by the clarification of the base sequences of these probes.