公开(公告)号：EP2562533A4

公开(公告)日：2017-12-06

申请号：EP11771919

申请日：2011-04-13

Applicant: HAMAMATSU PHOTONICS KK

Inventor： SUGIYAMA NORIKAZU ,  KATAOKA TAKUJI ,  IKEDA TAKAHIRO

IPC: G01N21/45 ,  C12Q1/02 ,  G01N21/25

CPC classification number: G01N21/45 ,  C12Q1/02 ,  G01N21/253

Abstract: A membrane potential change detection device 1 is provided with a reflection interference measurement light source 106, a holder 103 which holds a transparent member 102a on which cells 101 are mounted, a reflection interference detection camera 110 which images light emitted from the reflection interference measurement light source 106 and reflected from the cells 101 through the transparent member 102a, to generate a reflection interference image, and an analysis unit 202 which calculates a parameter dI about adhesion between the cells 101 and the transparent member 102a from the reflection interference image and detects a change of membrane potential of the cells 101 on the basis of a change of the parameter dI.

公开(公告)号：EP1734358A4

公开(公告)日：2008-03-26

申请号：EP05727784

申请日：2005-03-30

Applicant: HAMAMATSU PHOTONICS KK

Inventor： KATAOKA TAKUJI

IPC: G01N21/64

CPC classification number: G01N21/6428 ,  G01N21/6452 ,  G01N2021/6471 ,  G01N2021/6482

Abstract: A masking member in which an effect by fluorescence derived from fluorescent dye or a test compound in a buffer contained in a container together with an object to be measured is removed surely, and the need for removing excessive fluorescent dye or test compound contained in the buffer is eliminated. The masking member is employed when fluorescence of a measurement object arranged in the buffer within the container is measured from the bottom part of the container and comprises a light shielding part and an outer frame part. The light shielding part exhibits liquid permeability and intercepts background light from the buffer located on the side opposite to the bottom part of the container across the measurement object. While supporting the light shielding part, the outer frame part positions the light shielding part on the side opposite to the bottom part of the container across the measurement object. With such an arrangement, effect of background light derived from excessive fluorescent dye in the buffer is removed surely and the need for replacing process of the buffer is eliminated. The masking member is also applicable to the measurement of light emission.

公开(公告)号：EP0529084A4

公开(公告)日：1994-06-01

申请号：EP92904999

申请日：1992-02-13

Applicant: NIHON MILLIPORE KOGYO KK ,  HAMAMATSU PHOTONICS KK

Inventor： HIROSE ATSUMI ,  SETO SUSUMU ,  KATAOKA TAKUJI

IPC: C12Q1/06 ,  C12Q1/24 ,  C12Q1/66

CPC classification number: C12Q1/66 ,  C12Q1/06 ,  C12Q1/24 ,  Y10S435/975

公开(公告)号：EP2413127A4

公开(公告)日：2017-11-08

申请号：EP10755740

申请日：2010-01-26

Applicant: HAMAMATSU PHOTONICS KK

Inventor： KATAOKA TAKUJI ,  ITO TAIRA

IPC: G01N21/01 ,  G01N21/03 ,  G01N21/64

CPC classification number: G01N21/6452

公开(公告)号：EP2562246A4

公开(公告)日：2017-06-07

申请号：EP11771922

申请日：2011-04-13

Applicant: HAMAMATSU PHOTONICS KK

Inventor： SUGIYAMA NORIKAZU ,  KATAOKA TAKUJI ,  IKEDA TAKAHIRO

IPC: C12M1/34 ,  C12Q1/02 ,  G01B21/20 ,  G01N21/41 ,  G01N21/45 ,  G01N21/47 ,  G02B21/14 ,  G02B21/34

CPC classification number: G01N21/55 ,  C12M41/46 ,  G01N21/45 ,  G01N21/4788 ,  G02B21/14 ,  G02B21/34

Abstract: A cell observation device is provided with a reflection interference measurement light source 106, a quantitative phase measurement light source 105, a reflection interference detection camera 110 which images light emitted from the reflection interference measurement light source 106 and reflected from cells 101, to generate a reflection interference image, a quantitative phase detection camera 112 which images light emitted from the quantitative phase measurement light source 105 and transmitted by the cells 101, to generate a quantitative phase image, an image alignment unit 201 which matches a spatial position of the reflection interference image with a spatial position of the quantitative phase image, to implement alignment between the two images, a first extraction unit 204 which extracts a first parameter from the reflection interference image in alignment with the quantitative phase image, and a second extraction unit 205 which extracts a second parameter from the quantitative phase image in alignment with the reflection interference image.

Abstract translation: 细胞观察装置具有反射干涉测量光源106，定量相位测量光源105，对从反射干涉测量光源106发射并从细胞101反射的光成像的反射干扰检测摄像机110，以生成 反射干涉图像;定量相位检测摄像机112，其对从定量相位测量光源105发出并由单元101发送的光进行成像，以生成定量相位图像;图像对准单元201，其匹配反射干涉的空间位置 图像与定量相位图像的空间位置进行比较以实现两个图像之间的对准;第一提取单元204，其从与定量相位图像对齐的反射干涉图像中提取第一参数;以及第二提取单元205， 来自定量的第二个参数 相位图像与反射干涉图像对齐。

公开(公告)号：EP2955505A4

公开(公告)日：2016-11-02

申请号：EP14748553

申请日：2014-02-05

Applicant: HAMAMATSU PHOTONICS KK

Inventor： IWAI HIDENAO ,  KATAOKA TAKUJI ,  YAMAMOTO SATOSHI ,  SUGIYAMA NORIKAZU ,  YAMAUCHI TOYOHIKO ,  GOTO KENTARO

IPC: G01N21/47 ,  G01B11/00 ,  G01N21/17 ,  G01N21/49 ,  G01P3/36

CPC classification number: G01N33/4833 ,  G01B11/00 ,  G01N21/47 ,  G01N21/49 ,  G01N2021/4707 ,  G01N2021/479

Abstract: An aggregated cell evaluation apparatus 1 includes a laser light source 10, a speckle image acquisition unit 20, an SC calculation unit 30, an evaluation unit 40, and a memory unit 50. The speckle image acquisition unit 20 acquires a two-dimensional speckle image by forward scattered light generated in aggregated cells 90 by irradiation of the aggregated cells 90 with laser light output from the laser light source 10. The SC calculation unit 30 calculates a speckle contrast value K n of a speckle image In at each time t n , determines a maximum value K max among the speckle contrast values K 1 to K N , and normalizes the speckle contrast value K n at each time t n by the maximum value K max to obtain a normalized speckle contrast value K n '. The evaluation unit 40 evaluates motion of the aggregated cells 90, based on the normalized speckle contrast value K n ' at each time t n . This realizes a method and an apparatus capable of readily evaluating the motion of aggregated cells.

公开(公告)号：EP2725346A4

公开(公告)日：2015-04-08

申请号：EP12802697

申请日：2012-06-19

Applicant: HAMAMATSU PHOTONICS KK

Inventor： KATAOKA TAKUJI ,  MATSUBARA MASANORI

IPC: G06T7/00 ,  G01N21/64

CPC classification number: G02B21/365 ,  G01N21/6452 ,  G01N21/6458 ,  G06K9/0014 ,  G06T7/0016 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30072

公开(公告)号：JP2010190796A

公开(公告)日：2010-09-02

申请号：JP2009036945

申请日：2009-02-19

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI

IPC: G01N21/64 ,  G01N21/03 ,  G01N21/76

Abstract: PROBLEM TO BE SOLVED: To provide a background light reducing member and a light measuring method for reducing effectively background light generated in liquid such as a buffer existing around a measuring object without exerting an influence on the measuring object, even when a container has a small size, and dispensing with replacement of the buffer.
SOLUTION: This background light reducing member 1 is a member used for reducing background light generated in the buffer B around the measuring object S, when measuring through a bottom part of the container 40, light emitted from the measuring object S arranged in the buffer B in the bottomed container 40 by taking a fluorescent or light-emitting material. The member includes a hydrophobic contact part 10 inserted into the container 40 to be in contact with the buffer B and arranged so as to enclose a prescribed axis C along an insertion direction, and reduces background light owing to contact between the buffer B and the contact part 10 in the container 40.
COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：为了提供一种背景减轻部件和光测量方法，用于有效地减少存在于测量对象周围的缓冲液等液体中产生的背景光，而不会对测量对象产生影响，即使当容器 具有小尺寸，并且分配更换缓冲器。 解决方案：该背景减轻部件1是用于减少在测量对象S周围的缓冲器B中产生的背景光的部件，当通过容器40的底部进行测量时，从被布置在其中的测量对象S发射的光 通过采用荧光或发光材料在有底容器40中的缓冲液B。 构件包括插入到容器40中以与缓冲器B接触并且布置成沿插入方向包围规定轴线C的疏水接触部分10，并且由于缓冲器B和触点之间的接触而减少背景光 第10部分在集装箱40中。版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2008082772A

公开(公告)日：2008-04-10

申请号：JP2006261048

申请日：2006-09-26

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KOIKE YUKIKAZU ,  TANABE YASUYUKI ,  MIZUGUCHI YOSHINORI ,  KATAOKA TAKUJI ,  OZEKI HIDEO

IPC: G01N35/02 ,  G01N35/08 ,  G01N37/00

Abstract: PROBLEM TO BE SOLVED: To provide a chip holder capable of holding a channel chip, and circulating more easily sample liquid in a channel in the channel chip.
SOLUTION: This chip holder 1A holds the channel chip 40A having the channel 43 inside, wherein a liquid introduction hole 44 for introducing the sample liquid W into the channel and a liquid discharge hole 45 for discharging the sample liquid from the channel are formed. The chip holder 1A includes a base member 10 on which the channel chip is placed; a well member 20 mounted on the base member, pressing the channel chip, and having a well 26 for waiting the sample liquid supplied into the liquid introduction hole; and a cover member 30 mounted on the base member, pressing the channel chip, and having the first pipe 33 connected to the liquid discharge hole and the second pipe 37 inserted into the well 26. The well member 20 has a connection member 27 for connecting the well to the liquid introduction hole and allowing the sample liquid in the well to flow into the liquid introduction hole.
COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：提供一种能够保持通道芯片的芯片保持器，并且更容易地在通道芯片的通道中循环采样液体。 解决方案：该芯片保持器1A将具有通道43的通道芯片40A保持在内部，其中用于将样品液体W引入通道的液体引入孔44和用于从通道排出样品液体的液体排出孔45 形成。 芯片保持器1A包括其上放置有通道芯片的基底构件10; 安装在基座构件上的井构件20，按压通道芯片，并具有用于等待供应到液体引入孔中的样品液体的阱26; 以及安装在基座构件上的盖构件30，按压通道芯片，并且具有连接到液体排出孔的第一管33和插入到井26中的第二管37.阱构件20具有用于连接的连接构件27 该井到液体引入孔并允许井中的样品液体流入液体引入孔。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2011232056A

公开(公告)日：2011-11-17

申请号：JP2010100284

申请日：2010-04-23

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： SUGIYAMA NORIKAZU ,  KATAOKA TAKUJI ,  IKEDA TAKAHIRO

IPC: G01N21/27 ,  G01N21/03

CPC classification number: G01N21/45 ,  C12Q1/02 ,  G01N21/253

Abstract: PROBLEM TO BE SOLVED: To provide an apparatus and a method for detecting a change in a membrane potential capable of detecting the change in the membrane potential of a cell in a noninvasive method without labeling the cell membrane.SOLUTION: The apparatus for detecting the change in the membrane potential 1 includes: a light source 106 for reflection interference measurement; a holding section 103 for holding a transparent member 102a mounted with a cell 101; a camera 110 for reflection interference detection for imaging the reflected light which has been emitted from the light source 106 for reflection interference measurement and has been reflected from the cell 101 through the transparent member 102a to form a reflection interference image; and an analysis section 202 for calculating a parameter dI concerning adhesion between the cell 101 and the transparent member 102a from the reflection interference image and detecting the change in the membrane potential of the cell 101 based on the change in the parameter dI.

Abstract translation: 要解决的问题：提供一种用于检测能够以非侵入性方法检测细胞膜电位变化而不标记细胞膜的膜电位变化的装置和方法。 解决方案：用于检测膜电位1的变化的装置包括：用于反射干涉测量的光源106; 用于保持安装有单元101的透明构件102a的保持部103; 用于对从光源106发射的用于反射干涉测量的反射光成像的反射干涉检测用摄像机110，并通过透明构件102a从单元101反射，形成反射干涉图像; 以及分析部202，用于根据反射干涉图像计算关于单元101和透明构件102a之间的粘附的参数dI，并且基于参数dI的变化来检测单元101的膜电位的变化。 版权所有（C）2012，JPO＆INPIT