公开(公告)号：CA2307123A1

公开(公告)日：1999-05-06

申请号：CA2307123

申请日：1998-10-27

Applicant: IDEXX LAB INC

Inventor： NAQUI ALI ,  GELARDI PAUL J ,  PIERSON MARK W ,  TOWNSEND DAVID E ,  GU HAOYI

IPC: C12M1/34 ,  B01J19/00 ,  B01L3/00 ,  B01L3/02 ,  C12Q1/04 ,  G01N33/18 ,  G01N33/48 ,  G01N33/53 ,  G01N33/543

Abstract: Method and assay devices for the detection of the presence or amount of biological material, analyte(s), or microorganism(s) in a sample. The method includes the steps of liquefying the sample (if necessary) and distributing the liquefied sample over the surface of the assay device. The device may comprise an incubation plate, a dip stick device, or other devices. The devices have at least one reagent provided within the devices. Some devices have a generally flat horizontal surface which is divided into a plurality of recessed wells. Others have one or more surfaces with reagent island(s) immobilized thereon. Each well or reagent island is adapted to hold an aliquot of liquid. The wells or reagent islands are sized and shaped, and formed of a suitable material, to hold the aliquot within the well or reagent island by surface tension. Any excess liquid from the liquefied sample is drained from the surface of the device. The method then involves incubating the assay device until the presence or amount of the biological material, analyte, or microorganism is determined.

公开(公告)号：CA2223451A1

公开(公告)日：1996-12-19

申请号：CA2223451

申请日：1996-05-31

Applicant: IDEXX LAB INC

Inventor： CHEN CHUN-MING ,  TOWNSEND DAVID E

IPC: G01N33/02 ,  C12Q1/04 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/40 ,  C12Q1/42 ,  C12Q1/44

Abstract: This invention relates to a method for detecting the existence or measuring the concentration of total viable bacteria in a test sample from a food product. A medium is provided which contains three or more different enzyme substrates each having a nutrient moiety and a detectable moiety linked together. When a substrate is hydrolysed by a bacterial enzyme to create a separate detectable moiety, it causes or produces a detectable signal. These substrates produce detectable signals when any one of a phosphatase enzyme, a glycosidase enzyme or a peptidase enzyme is present in the medium.

公开(公告)号：DE69535747D1

公开(公告)日：2008-06-05

申请号：DE69535747

申请日：1995-10-27

Applicant: IDEXX LAB INC

Inventor： EHRENFELD ELIZABETH ,  FRICKER COLIN ,  TOWNSEND DAVID E

IPC: C12Q1/10 ,  C12Q1/20 ,  C12N1/20 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/54

Abstract: The invention features a medium for the detection of target microbes in a liquified environmental or biological sample in less than 18 hours.

公开(公告)号：CA2307123C

公开(公告)日：2007-04-24

申请号：CA2307123

申请日：1998-10-27

Applicant: IDEXX LAB INC

Inventor： PIERSON MARK W ,  TOWNSEND DAVID E ,  GELARDI PAUL J ,  NAQUI ALI ,  GU HAOYI

IPC: B01L3/00 ,  C12M1/34 ,  B01J19/00 ,  B01L3/02 ,  C12Q1/04 ,  G01N33/18 ,  G01N33/48 ,  G01N33/53 ,  G01N33/543

Abstract: Method and assay devices for the detection of the presence or amount of biological material, analyte(s), or microorganism(s) in a sample. The method includes the steps of liquefying th e sample (if necessary) and distributing the liquefied sample over the surface of the assay device. The device may comprise an incubation plate, a dip stick device, or other devices. The devices have at least one reagent provided within the devices. Some devices have a generally flat horizontal surface which is divided into a plurality of recessed wells. Others have one or more surfaces with reagent island(s) immobilized thereon. Each well or reagent island is adapted to hold an aliquot of liquid. The wells or reagent islands are sized and shaped, and formed of a suitable material, to hold the aliquot within the well or reagent island by surface tension. Any excess liquid from the liquefied sample is drained from the surface of the device. The method then involves incubating the assay device until the presence or amount of the biological material, analyte, or microorganism is determined.

公开(公告)号：AU756412B2

公开(公告)日：2003-01-09

申请号：AU1200099

申请日：1998-10-27

Applicant: IDEXX LAB INC

Inventor： PIERSON MARK W ,  TOWNSEND DAVID E ,  GU HAOYI ,  NAQUI ALI

IPC: C12M1/34 ,  B01J19/00 ,  B01L3/00 ,  B01L3/02 ,  C12Q1/04 ,  G01N33/18 ,  G01N33/48 ,  G01N33/53 ,  G01N33/543 ,  C12Q1/00 ,  G01N33/569

Abstract: Method and assay devices for the detection of the presence or amount of biological material, analyte(s), or microorganism(s) in a sample. The method includes the steps of liquefying the sample (if necessary) and distributing the liquefied sample over the surface of the assay device. The device may comprise an incubation plate, a dip stick device, or other devices. The devices have at least one reagent provided within the devices. Some devices have a generally flat horizontal surface which is divided into a plurality of recessed wells. Others have one or more surfaces with reagent island(s) immobilized thereon. Each well or reagent island is adapted to hold an aliquot of liquid. The wells or reagent islands are sized and shaped, and formed of a suitable material, to hold the aliquot within the well or reagent island by surface tension. Any excess liquid from the liquefied sample is drained from the surface of the device. The method then involves incubating the assay device until the presence or amount of the biological material, analyte, or microorganism is determined.

公开(公告)号：NZ504223A

公开(公告)日：2001-10-26

申请号：NZ50422398

申请日：1998-10-27

Applicant: IDEXX LAB INC

Inventor： NAQUI ALI ,  PIERSON MARK W ,  TOWNSEND DAVID E ,  GU HAOYI

IPC: C12M1/34 ,  B01J19/00 ,  B01L3/00 ,  B01L3/02 ,  C12Q1/04 ,  G01N33/18 ,  G01N33/48 ,  G01N33/53 ,  G01N33/543 ,  C12Q1/00

Abstract: Assay devices and method for the detection of the presence or amount of biological material, analyte(s), or microorganism(s) in a sample. The assay devices generally have a flat horizontal surface that is divided into a plurality of recessed wells; or one or more surfaces with reagent island(s) immobilized thereon. Each well or reagent island is formed of a suitable material, and is adapted in shape and size to hold an aliquot within the well or reagent island by surface tension. The devices have at least one reagent provided within the wells or reagent islands. The assay method includes the steps of liquefying the sample (if necessary) and distributing the liquefied sample over the surface of the assay device. Any excess liquid from the liquefied sample is drained from the surface of the device. The method then involves incubating the assay device until the presence or amount of the biological material, analyte, or microorganism is determined.

公开(公告)号：AU736154B2

公开(公告)日：2001-07-26

申请号：AU1281299

申请日：1998-10-27

Applicant: IDEXX LAB INC

Inventor： PIERSON MARK W ,  TOWNSEND DAVID E ,  GU HAOYI ,  NAQUI ALI ,  GELARDI PAUL J

IPC: C12M1/34 ,  B01J19/00 ,  B01L3/00 ,  B01L3/02 ,  C12Q1/04 ,  G01N33/18 ,  G01N33/48 ,  G01N33/53 ,  G01N33/543

Abstract: Method and assay devices for the detection of the presence or amount of biological material, analyte(s), or microorganism(s) in a sample. The method includes the steps of liquefying the sample (if necessary) and distributing the liquefied sample over the surface of the assay device. The device may comprise an incubation plate, a dip stick device, or other devices. The devices have at least one reagent provided within the devices. Some devices have a generally flat horizontal surface which is divided into a plurality of recessed wells. Others have one or more surfaces with reagent island(s) immobilized thereon. Each well or reagent island is adapted to hold an aliquot of liquid. The wells or reagent islands are sized and shaped, and formed of a suitable material, to hold the aliquot within the well or reagent island by surface tension. Any excess liquid from the liquefied sample is drained from the surface of the device. The method then involves incubating the assay device until the presence or amount of the biological material, analyte, or microorganism is determined.

公开(公告)号：AU723048B2

公开(公告)日：2000-08-17

申请号：AU7678396

申请日：1996-11-12

Applicant: IDEXX LAB INC

Inventor： CROTEAU ANDREW J ,  PIERSON MARK W ,  TOWNSEND DAVID E ,  NAQUI ALI

IPC: G01N33/48 ,  B01L3/00 ,  C12M1/20 ,  C12M1/34 ,  G01N1/00

Abstract: Method for detection of a biological material in a sample. The method includes the steps of liquifying the sample (if necessary) and pouring the liquified sample into the incubation plate. The incubation plate has a generally flat horizontal surface and the surface is divided into a plurality of at least 20 recessed wells. Each well is adapted to hold an aliquot of liquid and is sized and shaped, and formed of a suitable material, to hold the aliquot within the well by surface tension. Any excess liquid from the liquified sample is poured from the surface of the plate. The method then involves incubating that incubation plate until the presence or absence of the biological material is determined.

公开(公告)号：AU1200099A

公开(公告)日：1999-05-17

申请号：AU1200099

申请日：1998-10-27

Applicant: IDEXX LAB INC

Inventor： PIERSON MARK W ,  TOWNSEND DAVID E ,  GU HAOYI ,  NAQUI ALI

IPC: C12M1/34 ,  B01J19/00 ,  B01L3/00 ,  B01L3/02 ,  C12Q1/04 ,  G01N33/18 ,  G01N33/48 ,  G01N33/53 ,  G01N33/543 ,  C12Q1/00

Abstract: Method and assay devices for the detection of the presence or amount of biological material, analyte(s), or microorganism(s) in a sample. The method includes the steps of liquefying the sample (if necessary) and distributing the liquefied sample over the surface of the assay device. The device may comprise an incubation plate, a dip stick device, or other devices. The devices have at least one reagent provided within the devices. Some devices have a generally flat horizontal surface which is divided into a plurality of recessed wells. Others have one or more surfaces with reagent island(s) immobilized thereon. Each well or reagent island is adapted to hold an aliquot of liquid. The wells or reagent islands are sized and shaped, and formed of a suitable material, to hold the aliquot within the well or reagent island by surface tension. Any excess liquid from the liquefied sample is drained from the surface of the device. The method then involves incubating the assay device until the presence or amount of the biological material, analyte, or microorganism is determined.

公开(公告)号：MX9803774A

公开(公告)日：1998-09-30

申请号：MX9803774

申请日：1996-11-12

Applicant: IDEXX LAB INC

Inventor： CROTEAU ANDREW J ,  PIERSON MARK W ,  TOWNSEND DAVID E ,  NAQUI ALI

IPC: G01N33/48 ,  B01L3/00 ,  C12M1/20 ,  C12M1/34 ,  G01N1/00 ,  G01N01/00 ,  B01L03/00 ,  C12M01/20

Abstract: Se describe un método para la deteccion de un material biologico en una muestra; el método los pasos de liquidificar la muestra (si es necesario) y vaciar la muestra liquidificada en la placa de incubacion; la placa de incubacion tiene una superficie horizontal generalmente plana y la superficie está dividida en una pluralidad de por lo menos 20 cavidades hundidas; cada cavidad está adaptada para retener un alícuota de líquido y está configurada y conformada de un material adecuado para retener el alícuota dentro de la cavidad por tension de superficie; cualquier exceso de líquido que provenga de la muestra liquidificada es vaciado de la superficie de la placa; el método incluye también incubar esa placa de incubacion hasta que se determine la presencia o ausencia del material biologico.