Abstract:
PROBLEM TO BE SOLVED: To provide a process for determining the presence and quantity of biomaterial, specimen or microorganism in a sample and provide an apparatus therefor. SOLUTION: This process contains (if necessary) a step to liquefy a sample and a step to distribute the liquefied sample on the surface of an analyzing apparatus. The apparatus may contain an incubation plate 10, a dip stick or other device. The apparatus holds at least one reagent supplied to the apparatus. Some apparatuses have a nearly flat horizontal surface divided into plural concave wells. The excess liquid of the liquefied sample is discharged from the surface of the apparatus. This process contains a step to incubate the analyzing apparatus until the presence or the quantity of the biomaterial, specimen or microorganism is determined.
Abstract:
PROBLEM TO BE SOLVED: To provide a medium for detection of the target microbes in a liquified environmental or biological sample in less than 24 hours. SOLUTION: The target microbe-specific medium operable to allow viability and reproduction of the target microbe are obtained by comprising an effective amount of vitamin, amino acid, minor elements and salt ingredients. And the invention relates to use of sodium pyruvate to assist recovery of metabolically injured bacteria, use of thermal equilibrium period, use of a small amount of sodium chloride, offers a proper amount of other metabolite and a compound generating a specific detectable signal. COPYRIGHT: (C)2004,JPO
Abstract:
Method and assay devices for the detection of the presence or amount of biological material, analyte(s), or microorganism(s) in a sample. The method includes the steps of liquefying the sample (if necessary) and distributing the liquefied sample over the surface of the assay device. The device may comprise an incubation plate, a dip stick device, or other devices. The devices have at least one reagent provided within the devices. Some devices have a generally flat horizontal surface which is divided into a plurality of recessed wells. Others have one or more surfaces with reagent island(s) immobilized thereon. Each well or reagent island is adapted to hold an aliquot of liquid. The wells or reagent islands are sized and shaped, and formed of a suitable material, to hold the aliquot within the well or reagent island by surface tension. Any excess liquid from the liquefied sample is drained from the surface of the device. The method then involves incubating the assay device until the presence or amount of the biological material, analyte, or microorganism is determined.
Abstract:
The invention features a medium for the detection of target microbes in particular coliform, in a liquified environmental or biological sample in less than 24 hours.
Abstract:
The invention features a medium for the detection of target microbes in a liquified environmental or biological sample in less than 18 hours.
Abstract:
The invention features a medium for the detection of target microbes in a liquified environmental or biological sample in less than 18 hours.
Abstract:
This invention relates to a method for detecting the existence or measuring the concentration of total viable bacteria in a test sample from a food product. A medium is provided which contains three or more different enzyme substrates each having a nutrient moiety and a detectable moiety linked together. When a substrate is hydrolysed by a bacterial enzyme to create a separate detectable moiety, it causes or produces a detectable signal. These substrates produce detectable signals when any one of a phosphatase enzyme, a glycosidase enzyme or a peptidase enzyme is present in the medium.