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    ASSAY METHOD USING SURFACE PLASMON-FIELD ENHANCED FLUORESCENCE SPECTROSCOPY
    12.
    发明公开
    ASSAY METHOD USING SURFACE PLASMON-FIELD ENHANCED FLUORESCENCE SPECTROSCOPY 审中-公开
    与表面等离子体场增强荧光光谱测试程序

    公开(公告)号：EP2910947A4

    公开(公告)日：2016-06-08

    申请号：EP13848099

    申请日：2013-10-17

    Applicant: KONICA MINOLTA INC

    Inventor： KAYA TAKATOSHI ,  OOTANI MAKIKO

    IPC: G01N33/543 ,  G01N21/64 ,  G01N21/78 ,  G01N33/569

    CPC classification number: G01N21/648 ,  G01N33/54306 ,  G01N33/56966 ,  G01N2021/6439

    Abstract: Provided is an assay method utilizing surface plasmon-field enhanced fluorescence spectroscopy, which suppresses fluctuations in an absolute value of a background signal that is referred to as a resonance angle or an optical blank by improving the effect when a whole blood sample is used, specifically, the effect by the blood cell components remaining in a measurement liquid during a SPFS measurement, with regards to a photometric condition setting step, which is a characteristic step in assay methods utilizing SPFS. The above-mentioned problem is solved by an assay method utilizing surface plasmon-field enhanced fluorescence spectroscopy, including a primary reaction step of a sample, a first washing step, a labeling reaction step, and a measurement step, wherein the method includes a step for evaluating a blood cell component quantity between the primary reaction step and the labeling reaction step.

    DETECTION CHIP AND DETECTION METHOD
    16.
    发明公开
    DETECTION CHIP AND DETECTION METHOD 审中-公开

    公开(公告)号：EP3232182A4

    公开(公告)日：2018-03-21

    申请号：EP15868386

    申请日：2015-11-20

    Applicant: KONICA MINOLTA INC

    Inventor： NAGAE KOSUKE ,  KAYA TAKATOSHI ,  NAKAMURA YUKITO

    IPC: G01N21/64

    CPC classification number: G01N21/64 ,  G01N21/648

    Abstract: The detection chip according to the present invention has an accommodating part, a metal film, a first reaction field, and a second reaction field. The accommodating part accommodates a liquid. The metal film is arranged in a bottom part of the accommodating part so that one face thereof faces into the accommodating part. The first reaction field and the second reaction field are arranged in mutually different regions on one face of the metal film. A capture body is immobilized in the first reaction field and the second reaction field. When the liquid is accommodated in the accommodating part, the depth of the liquid on the first reaction field differs from the depth of the liquid on the second reaction field.

    SANDWICH ASSAY USING LABELED LECTIN AND KIT THEREFOR
    17.
    发明公开
    SANDWICH ASSAY USING LABELED LECTIN AND KIT THEREFOR 审中-公开

    公开(公告)号：EP3159694A4

    公开(公告)日：2018-01-17

    申请号：EP15809575

    申请日：2015-06-01

    Applicant: KONICA MINOLTA INC

    Inventor： KANEKO TOMONORI ,  KAYA TAKATOSHI

    IPC: G01N33/53 ,  G01N33/50 ,  G01N33/543 ,  G01N33/574

    CPC classification number: G01N33/54393 ,  G01N33/50 ,  G01N33/57484 ,  G01N2333/42 ,  G01N2333/4724 ,  G01N2333/4728 ,  G01N2400/00 ,  G01N2400/02 ,  G01N2440/38

    Abstract: The present invention provides a sandwich assay for quantifying a glycoprotein, which is a substance to be detected, in a sample using a labeled lectin, wherein the effect attributed to a contaminant, namely noise on the quantified value of the substance to be detected, is suppressed by introduction of a simple treatment. The sandwich assay includes a treatment for inhibiting the binding of the labeled lectin to a sugar chain carried by the contaminant non-specifically adsorbed to the measurement region, which contaminant is contained in the sample and which sugar chain is the same as that of the substance to be detected. When the contaminant is a glycoprotein having the same sugar chain as the substance to be detected, the treatment for inhibiting the binding of the laveled lectin to the sugar chain is preferably a treatment of adding a substance showing an action of cleaving disulfide bonds of the contaminant to the sample and thereby dissociating, from the contaminant, a domain containing the same sugar chain as that of the substance to be detected.

    SURFACE PLASMON-FIELD ENHANCED FLUORESCENCE MEASUREMENT DEVICE AND FLUORESCENCE DETECTION METHOD USING SAME
    18.
    发明公开
    SURFACE PLASMON-FIELD ENHANCED FLUORESCENCE MEASUREMENT DEVICE AND FLUORESCENCE DETECTION METHOD USING SAME 审中-公开
    FLERORAZENZNAZWEISVERFAHREN DAMIT IT TE IT IT IT IT IT IT IT IT IT IT IT IT IT IT IT IT IT IT IT IT IT

    公开(公告)号：EP2711689A4

    公开(公告)日：2015-01-07

    申请号：EP12785616

    申请日：2012-04-23

    Applicant: KONICA MINOLTA INC

    Inventor： KAYA TAKATOSHI

    IPC: G01N21/64

    CPC classification number: G01N21/6428 ,  G01N21/6452 ,  G01N21/648

    Abstract: It is an object to provide a surface plasmon-field enhanced fluorescence measurement device capable of accurately detecting a specific substance even in the case in which a well member is used and achieving a simpler structure and a lower manufacturing cost, and a fluorescence detection method using the surface plasmon-field enhanced fluorescence measurement device. [Solution of Problem] A surface plasmon-field enhanced fluorescence measurement device is provided with: a sensor structure that is configured from a sensor member that is provided with a dielectric member, a metallic thin film formed on the dielectric member, and a ligand immobilized region formed on the metallic thin film, and a well member affixed onto the sensor member and having a through hole at a position that is corresponded to the ligand immobilized region; and a device body that is provided with at least a light source for applying excitation light to the metallic thin film of the sensor structure, and a light detection means for exciting a fluorescent substance held in the ligand immobilized region on the metallic thin film by applying the excitation light to the metallic thin film from the light source to enhance an electric field on the metallic thin film, and detecting fluorescence that has been excited, and the sensor structure is configured so as to be used attachably to and detachably from the device body or so as to be used while being affixed to the device body. The application position of the excitation light is set in such a manner that the center of the optical axis of the excitation light is located at a position a predetermined distance away from the position of the center of gravity of a bottom surface region that is defined by the through hole of the well member of the sensor structure.

    Abstract translation: 本发明的目的是提供一种能够精确地检测特定物质的表面等离子体场增强型荧光测量装置，即使在使用阱构件的情况下也能够精确地检测特定物质，并且实现更简单的结构和更低的制造成本，并且使用 表面等离子体场增强荧光测量装置。 [问题的解决方案]表面等离子体增强荧光测量装置具有：传感器结构，其由设置有电介质构件的传感器构件，形成在电介质构件上的金属薄膜和固定有配体的配体构成 形成在金属薄膜上的区域，以及固定在传感器构件上并且在与配体固定区域对应的位置处具有通孔的阱构件; 以及设置有至少一个用于向传感器结构的金属薄膜施加激发光的光源的装置体，以及光检测装置，用于通过施加在金属薄膜上激发保持在金属薄膜上的配体固定区域中的荧光物质 激发光从光源到金属薄膜，以增强金属薄膜上的电场，并检测已被激发的荧光，并且传感器结构被配置为可以被附接到装置主体和可拆卸地使用 或者在被固定在装置主体上时被使用。 激发光的施加位置被设定为使得激发光的光轴的中心位于与底面区域的重心位置相距预定距离的位置，该位置由下表面区域的重心定义 传感器结构的井部件的通孔。

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