公开(公告)号：SG11201706332RA

公开(公告)日：2017-09-28

申请号：SG11201706332R

申请日：2016-02-05

Applicant: LIFE TECHNOLOGIES CORP

Inventor： CHU YONG ,  MARKS JEFFREY ,  FREUDENTHAL JACOB ,  WESSEL THOMAS ,  WOO DAVID

IPC: G01N21/27 ,  C12Q1/68 ,  G01N21/64

Abstract: In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging fluorescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.