公开(公告)号：US11319587B2

公开(公告)日：2022-05-03

申请号：US16292087

申请日：2019-03-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang Hinz ,  David Light ,  Todd Rearick ,  James A. Ball

IPC: C12Q1/6874 ,  G01N27/414

Abstract: The disclosure relates to novel particle compositions and methods of making said compositions having applications in nucleic acid analysis, as well as apparatuses and systems for the same.

公开(公告)号：US10066260B2

公开(公告)日：2018-09-04

申请号：US14774662

申请日：2014-03-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David Light ,  Wolfgang Hinz ,  Ronald L. Cicero ,  Christina E. Inman ,  Paul M. Kenney ,  Alexander Mastroianni ,  Roman Rozhkov ,  Yufang Wang ,  Jeremy Gray ,  Marc Glazer ,  Dmitriy Gremyachinskiy

IPC: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6869

CPC classification number: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12Q2523/101 ,  C12Q2527/119 ,  C12Q2535/122 ,  C12Q2547/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2523/301 ,  C12Q2523/32

Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

公开(公告)号：US20250161901A1

公开(公告)日：2025-05-22

申请号：US19029066

申请日：2025-01-17

Applicant: Life Technologies Corporation

Inventor： David Light ,  Ronald L. Cicero ,  Yufang Wang ,  Alexander Mastroianni ,  Prasanna Krishnan Thwar ,  Jeremy Gray ,  Marc Glazer ,  Tommie Lloyd Lincecum, JR. ,  Mohammad Alanjary ,  Joseph Koscinski

IPC: B01J19/00 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: A method of loading beads on a sensor substrate includes applying a suspension including beads to a flow cell defined over a sensor substrate. The sensor substrate includes a plurality of wells. The beads at least partially deposit into the plurality of wells. The method also includes removing liquid from the flow cell, evaporating liquid from the flow cell, for example, by drawing air through the flow cell; and applying a hydrating solution to the flow cell.

公开(公告)号：US20240318248A1

公开(公告)日：2024-09-26

申请号：US18733283

申请日：2024-06-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Guobin LUO ,  Marina Sedova ,  David Light ,  Ryan Jones ,  Mohammad Alanjary

IPC: C12Q1/6874 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6806

Abstract: A method for depositing particles on a surface includes receiving a plurality of particles, a particle of the plurality of particles having a polymer matrix conjugated to nucleic acid strands, exposing the plurality of particles to a solution; and applying the plurality of particles to a surface following exposing the plurality of particles to the solution, particles of the plurality of particles depositing on the surface. The solution includes a magnesium salt in a range of 30 mM to 500 mM and polyethylene glycol.

公开(公告)号：US12006543B2

公开(公告)日：2024-06-11

申请号：US17217492

申请日：2021-03-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Guobin Luo ,  Marina Sedova ,  David Light ,  Ryan Jones ,  Mohammad Alanjary

IPC: C12Q1/6874 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q2527/125 ,  C12Q2563/149 ,  C12Q1/6874 ,  C12Q2563/137 ,  C12Q2563/149 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q1/6806 ,  C12Q2535/122 ,  C12Q2563/137 ,  C12Q2563/149

Abstract: A method for depositing particles on a surface includes receiving a plurality of particles, a particle of the plurality of particles having a polymer matrix conjugated to nucleic acid strands, exposing the plurality of particles to a solution; and applying the plurality of particles to a surface following exposing the plurality of particles to the solution, particles of the plurality of particles depositing on the surface. The solution includes a magnesium salt in a range of 30 mM to 500 mM, a potassium salt in a range of 0.8 M to 1.0 M, a buffering agent in a range of 150 mM to 500 mM, and a surfactant in a range of 0.05% to 0.5%.

公开(公告)号：US09890423B2

公开(公告)日：2018-02-13

申请号：US15254193

申请日：2016-09-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David Light ,  Barnett B. Rosenblum

IPC: C12Q1/68 ,  C08F2/32 ,  C08F220/56 ,  C08F251/00 ,  C08F222/38

CPC classification number: C12Q1/6834 ,  C08F2/32 ,  C08F220/56 ,  C08F222/385 ,  C08F251/00 ,  C12Q1/6869 ,  C12Q2563/155 ,  C12Q2563/159

Abstract: The disclosure relates to methods of making polymer particles, said methods including the steps of: making an aqueous gel reaction mixture; forming an emulsion having dispersed aqueous phase micelles of gel reaction mixture in a continuous phase; adding an initiator oil comprising at least one polymerization initiator to the continuous phase; and performing a polymerization reaction in the micelles. Further, the initiator oil is present in a volume % relative to a volume of the aqueous gel reaction mixture of between about 1 vol % to about 20 vol %. The disclosure also relates to methods of making nucleic acid polymer particles having the same method steps and wherein the aqueous gel reaction mixture includes a nucleic acid fragment, such as a primer.

公开(公告)号：US20150080228A1

公开(公告)日：2015-03-19

申请号：US14485282

申请日：2014-09-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Guobin Luo ,  Marina Sedova ,  David Light ,  Ryan Jones ,  Mohammad Alanjary

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2563/149 ,  C12Q2563/137 ,  C12Q2535/122

Abstract: A method of sequencing a nucleic acid strand includes receiving particles having nucleic acid strands coupled to a polymer matrix, exposing the particles to a solution including a condensing agent, and applying the particles to a surface, the particles depositing on the surface.

Abstract translation: 对核酸链进行测序的方法包括接收具有与聚合物基质偶联的核酸链的颗粒，将颗粒暴露于包含缩合剂的溶液中，并将颗粒施用于表面，颗粒沉积在表面上。