公开(公告)号：AU2002359721A1

公开(公告)日：2003-07-09

申请号：AU2002359721

申请日：2002-12-16

Applicant: SQUIBB BRISTOL MYERS CO

Inventor： GOLDBERG STEVEN L ,  CINO PAUL M ,  PATEL RAMESH N ,  NANDURI VENKATA B ,  JOHNSTON ROBERT M

IPC: C12N9/02 ,  C12P21/04 ,  C12Q1/26

Abstract: This invention relates to a recombinant Pichia pastoris formate dehydrogenase (FDH) enzyme that catalyzes the oxidation of formate to carbon dioxide and the simultaneous reduction of nicotinamide adenine dinucleotide (NAD+) to its reduced form (NADH). Also related are isolated nucleic acids encoding P. pastoris FDH polypeptides, and fragments and variants thereof, as well as vectors and host cells comprising these nucleic acids. Further related are isolated, recombinant P. pastoris FDH polypeptides, and fragments and variants thereof, and antibodies that specifically bind to P. pastoris FDH polypeptides, fragments, or variants. The invention also relates to methods of obtaining isolated P. pastoris FDH nucleic acids, polypeptides, and antibodies, and methods of using P. pastoris FDH in various reactions for industrial or pharmaceutical applications.

公开(公告)号：AU5797699A

公开(公告)日：2000-03-27

申请号：AU5797699

申请日：1999-08-31

Applicant: SQUIBB BRISTOL MYERS CO

Inventor： PATEL RAMESH N ,  BANERJEE AMIT ,  NANDURI VENKATA B ,  GOLDBERG STEVEN L ,  JOHNSTON ROBERT M ,  TULLY THOMAS P ,  SZARKA LASZLO J ,  SWAMINATHAN SHANKAR ,  VENIT JOHN J ,  MONIOT JEROME L ,  WINTER WILLIAM J ,  ANDERSON NEAL G ,  LUST DAVID A ,  CRISPINO GERARD ,  SRIVASTAVA SUSHIL

IPC: C12N15/09 ,  A61K38/00 ,  C07D513/04 ,  C07K5/062 ,  C07K5/078 ,  C07K14/81 ,  C12N1/20 ,  C12N1/21 ,  C12N9/06 ,  C12N9/10 ,  C12P17/18 ,  C12R1/01 ,  C12R1/38 ,  C12R1/465 ,  C12P21/00 ,  C12P21/02

Abstract: The present invention concerns an enzymatic oxidative deamination process of a dipeptide monomer to prepare an intermediate useful to prepare compounds having endopeptidase and angiotensin converting enzyme inhibition activity.