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    DNA SEQUENCES FOR ENZYMATIC SYNTHESIS OF POLYKETIDE OR HETEROPOLYKETIDE COMPOUNDS
    1.
    发明申请
    DNA SEQUENCES FOR ENZYMATIC SYNTHESIS OF POLYKETIDE OR HETEROPOLYKETIDE COMPOUNDS 审中-公开
    多核苷酸或异黄酮化合物的酶促合成DNA序列

    公开(公告)号：WO0022139A3

    公开(公告)日：2001-01-18

    申请号：PCT/US9923535

    申请日：1999-10-11

    Applicant: BIOTECHNOLOG FORSCHUNG GMBH ,  SQUIBB BRISTOL MYERS CO ,  BEYER STEFAN ,  BLOECKER HELMUT ,  BRANDT PETRA ,  CINO PAUL M ,  DOUGHERTY BRIAN A ,  GOLDBERG STEVEN L ,  HOFLE GERHARD ,  MUELLER ROLF JOACHIM ,  REICHENBACH HANS

    Inventor： BEYER STEFAN ,  BLOECKER HELMUT ,  BRANDT PETRA ,  CINO PAUL M ,  DOUGHERTY BRIAN A ,  GOLDBERG STEVEN L ,  HOFLE GERHARD ,  MUELLER ROLF-JOACHIM ,  REICHENBACH HANS

    IPC: C12N15/09 ,  C12N1/21 ,  C12N9/00 ,  C12N15/52 ,  C12P1/04 ,  C12P7/26 ,  C12P7/62 ,  C12P17/18 ,  C12R1/01 ,  C07K14/195 ,  C12N5/10 ,  C12N15/63 ,  C12P17/06

    CPC classification number: C12N15/52 ,  C12P7/62 ,  C12P17/181

    Abstract: The invention consists of: (1) cloned Sorangium cellulosum polyketide synthase (PKS) biosynthetic cluster DNA; and (2) the nucleotide sequence and predicted protein coding sequences of the cloned DNA. The invention can be used for, but not limited to: (a) increasing yields of PKS product in Sorangium cellulosum (e.g., by amplification or genetic modification of the epothilone gene cluster or its component parts); (b) increasing yields of polyketide product in a heterologous system by transfer of the epothilone gene cluster or its component parts, which may be followed by amplification or genetic modification of the PKS gene cluster or its component parts; (c) modification of the polyketide product chemical structure in either Sorangium cellulosum or a heterologous host (e.g., by genetic modification of the epothilone gene cluster or its component parts; and (d) for the detection of genes and gene products involved in making polyketides or related molecules in other organisms (e.g., by hybridization or complementation assays). DNA sequence and analysis is presented for the following cosmids and plasmids: A2 cosmid; the pEPOcos6 region (overlapping of pEPOcos6 and pEPOcos7); pEPOcos8 cosmid; A5 cosmid; Sau4 (10 kb plasmid).

    Abstract translation: 本发明包括：（1）克隆的纤维素纤丝素聚酮合成酶（PKS）生物合成簇DNA; 和（2）克隆DNA的核苷酸序列和预测的蛋白质编码序列。 本发明可用于但不限于：（a）增加纤维素纤丝素中PKS产物的产量（例如通过埃坡霉素基因簇或其组分部分的扩增或遗传修饰）; （b）通过转移埃坡霉素基因簇或其组分部分增加异源系统中聚酮化合物产物的产量，其可以是PKS基因簇或其组分部分的扩增或遗传修饰。 （c）修饰纤丝素或异源宿主中的聚酮化合物产物化学结构（例如，通过埃坡霉素基因簇或其组分部分的遗传修饰;以及（d）检测涉及制备聚酮化合物的基因和基因产物 提供了以下粘粒和质粒的DNA序列和分析：A2粘粒; pEPOcos6区域（pEPOcos6和pEPOcos7的重叠）; pEPOcos8粘粒; A5粘粒; Sau4（例如，通过杂交或互补测定） （10kb质粒）。

    DNA SEQUENCES FOR ENZYMATIC SYNTHESIS OF POLYKETIDE OR HETEROPOLYKETIDE COMPOUNDS
    2.
    发明申请
    DNA SEQUENCES FOR ENZYMATIC SYNTHESIS OF POLYKETIDE OR HETEROPOLYKETIDE COMPOUNDS 审中-公开
    多核苷酸或异黄酮化合物的酶促合成DNA序列

    公开(公告)号：WO0022139A9

    公开(公告)日：2000-09-08

    申请号：PCT/US9923535

    申请日：1999-10-11

    Applicant: BIOTECHNOLOG FORSCHUNG GMBH ,  SQUIBB BRISTOL MYERS CO ,  BEYER STEFAN ,  BLOECKER HELMUT ,  BRANDT PETRA ,  CINO PAUL M ,  DOUGHERTY BRIAN A ,  GOLDBERG STEVEN L ,  HOFLE GERHARD ,  MUELLER ROLF JOACHIM ,  REICHENBACH HANS

    Inventor： BEYER STEFAN ,  BLOECKER HELMUT ,  BRANDT PETRA ,  CINO PAUL M ,  DOUGHERTY BRIAN A ,  GOLDBERG STEVEN L ,  HOFLE GERHARD ,  MUELLER ROLF-JOACHIM ,  REICHENBACH HANS

    IPC: C12N15/09 ,  C12N1/21 ,  C12N9/00 ,  C12N15/52 ,  C12P1/04 ,  C12P7/26 ,  C12P7/62 ,  C12P17/18 ,  C12R1/01 ,  C12N5/10 ,  C12N15/63 ,  C12P17/06

    CPC classification number: C12N15/52 ,  C12P7/62 ,  C12P17/181

    Abstract: The invention consists of: (1) cloned Sorangium cellulosum polyketide synthase (PKS) biosynthetic cluster DNA; and (2) the nucleotide sequence and predicted protein coding sequences of the cloned DNA. The invention can be used for, but not limited to: (a) increasing yields of PKS product in Sorangium cellulosum (e.g., by amplification or genetic modification of the epothilone gene cluster or its component parts); (b) increasing yields of polyketide product in a heterologous system by transfer of the epothilone gene cluster or its component parts, which may be followed by amplification or genetic modification of the PKS gene cluster or its component parts; (c) modification of the polyketide product chemical structure in either Sorangium cellulosum or a heterologous host (e.g., by genetic modification of the epothilone gene cluster or its component parts; and (d) for the detection of genes and gene products involved in making polyketides or related molecules in other organisms (e.g., by hybridization or complementation assays). DNA sequence and analysis is presented for the following cosmids and plasmids: A2 cosmid; the pEPOcos6 region (overlapping of pEPOcos6 and pEPOcos7); pEPOcos8 cosmid; A5 cosmid; Sau4 (10 kb plasmid).

    Abstract translation: 本发明包括：（1）克隆的纤维素纤丝素聚酮合成酶（PKS）生物合成簇DNA; 和（2）克隆DNA的核苷酸序列和预测的蛋白质编码序列。 本发明可用于但不限于：（a）增加纤维素纤丝素中PKS产物的产量（例如通过埃坡霉素基因簇或其组分部分的扩增或遗传修饰）; （b）通过转移埃坡霉素基因簇或其组分部分增加异源系统中聚酮化合物产物的产量，其可以是PKS基因簇或其组分部分的扩增或遗传修饰。 （c）修饰纤丝素或异源宿主中的聚酮化合物产物化学结构（例如，通过埃坡霉素基因簇或其组分部分的遗传修饰;以及（d）检测涉及制备聚酮化合物的基因和基因产物 提供了以下粘粒和质粒的DNA序列和分析：A2粘粒; pEPOcos6区域（pEPOcos6和pEPOcos7的重叠）; pEPOcos8粘粒; A5粘粒; Sau4（例如，通过杂交或互补测定） （10kb质粒）。

    PICHIA PASTORIS FORMATE DEHYDROGENASE AND USES THEREFOR
    3.
    发明申请
    PICHIA PASTORIS FORMATE DEHYDROGENASE AND USES THEREFOR 审中-公开
    PICHIA PASTORIS FORMATE DEHYDROGENASE及其用途

    公开(公告)号：WO03054155A2

    公开(公告)日：2003-07-03

    申请号：PCT/US0240235

    申请日：2002-12-16

    Applicant: SQUIBB BRISTOL MYERS CO ,  GOLDBERG STEVEN L ,  CINO PAUL M ,  PATEL RAMESH N ,  NANDURI VENKATA B ,  JOHNSTON ROBERT M

    Inventor： GOLDBERG STEVEN L ,  CINO PAUL M ,  PATEL RAMESH N ,  NANDURI VENKATA B ,  JOHNSTON ROBERT M

    IPC: C12N9/02 ,  C12P21/04 ,  C12Q1/26 ,  C12N

    CPC classification number: C12N9/0004 ,  C12Q1/26 ,  G01N2333/04

    Abstract: This invention relates to a recombinant Pichia pastoris formate dehydrogenase (FDH) enzyme that catalyzes the oxidation of formate to carbon dioxide and the simultaneous reduction of nicotinamide adenine dinucleotide (NAD+) to its reduced form (NADH). Also related are isolated nucleic acids encoding P. pastoris FDH polypeptides, and fragments and variants thereof, as well as vectors and host cells comprising these nucleic acids. Further related are isolated, recombinant P. pastoris FDH polypeptides, and fragments and variants thereof, and antibodies that specifically bind to P. pastoris FDH polypeptides, fragments, or variants. The invention also relates to methods of obtaining isolated P. pastoris FDH nucleic acids, polypeptides, and antibodies, and methods of using P. pastoris FDH in various reactions for industrial or pharmaceutical applications.

    Abstract translation: 本发明涉及催化甲酸氧化成二氧化碳并同时将烟酰胺腺嘌呤二核苷酸（NAD +）还原为还原形式（NADH）的重组巴斯德毕赤酵母甲酸脱氢酶（FDH）酶。 还涉及编码巴斯德毕赤酵母多肽的分离的核酸及其片段和变体，以及包含这些核酸的载体和宿主细胞。 进一步相关的是分离的，重组的巴斯德毕赤酵母多肽，及其片段和变体，以及特异性结合巴斯德毕赤酵母多肽，片段或变体的抗体。 本发明还涉及获得分离的巴斯德毕赤氏酵母多肽和多肽的抗体的方法，以及在工业或制药的各种反应中使用巴斯德毕赤酵母多肽的方法 应用。

    PICHIA PASTORIS FORMATE DEHYDROGENASE AND USES THEREFOR
    4.
    发明公开
    PICHIA PASTORIS FORMATE DEHYDROGENASE AND USES THEREFOR 审中-公开
    从毕赤酵母甲酸脱氢酶及其用途

    公开(公告)号：EP1463807A4

    公开(公告)日：2006-04-12

    申请号：EP02794274

    申请日：2002-12-16

    Applicant: SQUIBB BRISTOL MYERS CO

    Inventor： GOLDBERG STEVEN L ,  CINO PAUL M ,  PATEL RAMESH N ,  NANDURI VENKATA B ,  JOHNSTON ROBERT M

    IPC: C12N9/02 ,  C12P21/04 ,  C12Q1/26 ,  C12N9/04 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00

    CPC classification number: C12N9/0004 ,  C12Q1/26 ,  G01N2333/04

    Abstract: This invention relates to a recombinant Pichia pastoris formate dehydrogenase (FDH) enzyme that catalyzes the oxidation of formate to carbon dioxide and the simultaneous reduction of nicotinamide adenine dinucleotide (NAD+) to its reduced form (NADH). Also related are isolated nucleic acids encoding P. pastoris FDH polypeptides, and fragments and variants thereof, as well as vectors and host cells comprising these nucleic acids. Further related are isolated, recombinant P. pastoris FDH polypeptides, and fragments and variants thereof, and antibodies that specifically bind to P. pastoris FDH polypeptides, fragments, or variants. The invention also relates to methods of obtaining isolated P. pastoris FDH nucleic acids, polypeptides, and antibodies, and methods of using P. pastoris FDH in various reactions for industrial or pharmaceutical applications.

    Pichia pastoris formate dehydrogenase and uses therefor
    8.
    发明专利
    Pichia pastoris formate dehydrogenase and uses therefor 未知

    公开(公告)号：AU2002359721A8

    公开(公告)日：2003-07-09

    申请号：AU2002359721

    申请日：2002-12-16

    Applicant: SQUIBB BRISTOL MYERS CO

    Inventor： GOLDBERG STEVEN L ,  NANDURI VENKATA B ,  CINO PAUL M ,  PATEL RAMESH N ,  JOHNSTON ROBERT M

    IPC: C12N9/02 ,  C12P21/04 ,  C12Q1/26 ,  C12N9/04 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    Abstract: This invention relates to a recombinant Pichia pastoris formate dehydrogenase (FDH) enzyme that catalyzes the oxidation of formate to carbon dioxide and the simultaneous reduction of nicotinamide adenine dinucleotide (NAD+) to its reduced form (NADH). Also related are isolated nucleic acids encoding P. pastoris FDH polypeptides, and fragments and variants thereof, as well as vectors and host cells comprising these nucleic acids. Further related are isolated, recombinant P. pastoris FDH polypeptides, and fragments and variants thereof, and antibodies that specifically bind to P. pastoris FDH polypeptides, fragments, or variants. The invention also relates to methods of obtaining isolated P. pastoris FDH nucleic acids, polypeptides, and antibodies, and methods of using P. pastoris FDH in various reactions for industrial or pharmaceutical applications.

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