公开(公告)号：CA2809498C

公开(公告)日：2020-03-31

申请号：CA2809498

申请日：2011-08-26

Applicant: UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  CRAFT APRIL M ,  DUBOIS NICOLE C

IPC: C12N5/077 ,  C12N5/0775 ,  C12Q1/04 ,  G01N33/567

Abstract: The present invention relates to methods for enriching pluripotent stem cell-derived cardiomyocyte progenitor cells and cardiomyocyte cells based on SIRPA expression.

公开(公告)号：CA3111704A1

公开(公告)日：2020-03-26

申请号：CA3111704

申请日：2019-09-18

Applicant: UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  GAGE BLAIR KENNETH

IPC: C12N5/071 ,  A61K35/44 ,  A61P1/16 ,  C12N5/073 ,  C12Q1/02 ,  G01N33/15 ,  G01N33/48 ,  G01N33/53

Abstract: Disclosed herein are methods of producing a population of venous angioblast cells from stem cells using a venous angioblast inducing media and optionally isolating a CD34+ population from the cell population comprising the venous angioblast cells, for example using a CD34 affinity reagent, CD31 affinity reagent and/or CD144 affinity reagent, optionally with or without a CD73 affinity reagent as well as methods of further differentiating the venous angioblasts in vitro to produce SEC-LCs and/or in vivo to produce SECs. Uses of the cells and compositions comprising the cells are also described.

公开(公告)号：AU2014218290B2

公开(公告)日：2020-03-12

申请号：AU2014218290

申请日：2014-02-18

Applicant: HOSPITAL FOR SICK CHILDREN ,  UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  OGAWA SHINICHIRO ,  GHANEKAR ANAND ,  BEAR CHRISTINE ,  KAMATH BINITA M ,  OGAWA MINA ,  SURAPISITCHAT JAMES

IPC: C12N5/071 ,  A61K35/12 ,  A61K35/407 ,  A61P1/16 ,  C12N5/0735 ,  C12Q1/02

Abstract: Methods for producing hepatocyte and/or cholangiocyte lineage cells from pluripotent stem cells, the method comprising (a) specifying the extended nodal agonist treated induced endodermal cell population to obtain a cell population comprising hepatocyte and/or cholangiocyte progenitors by contacting the extended nodal agonist treated induced endodermal cell population with specification media comprising a FGF agonist and a BMP4 agonist and/or active conjugates and/or fragments thereof; (b) inducing maturation, and optionally further lineage specification and/or expansion of the hepatocyte and/or cholangiocyte progenitors of the cell population to obtain a population comprising hepatocyte lineage cells such as hepatoblasts, hepatocytes and/or cholangiocytes, the inducing maturation step comprising generating aggregates of the cell population. Optionally, the method also comprises activating the cAMP pathway within the aggregates and forming co-aggregates.

公开(公告)号：AU2017239493B2

公开(公告)日：2019-10-31

申请号：AU2017239493

申请日：2017-10-03

Applicant: UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  CRAFT APRIL M ,  DUBOIS NICOLE C

IPC: C12N5/077 ,  C12N5/0775 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/567

Abstract: The present invention relates to methods for enriching pluripotent stem cell-derived cardiomyocyte progenitor cells and cardiomyocyte cells based on SIRPA expression.

公开(公告)号：AU2017369684A1

公开(公告)日：2019-06-20

申请号：AU2017369684

申请日：2017-12-04

Applicant: UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  PROTZE STEPHANIE ,  LEE JEE HOON

IPC: C12N5/077 ,  A61K35/34 ,  A61P9/04 ,  C12N5/071 ,  C12Q1/02 ,  G01N33/48

Abstract: Methods are disclosed for producing populations of cardiomyocytes from pluripotent stem cells. Populations may be enriched for either atrial or ventricular cardiomyocytes and the resulting ventricular population may be essentially free of pacemaker cells. The method includes incubating pluripotent stem cells in a suitable medium with a BMP component, and an activin component, the amounts of activin may be varied to enrich for either atrial or ventricular cardiomyocytes. The enriched populations, as well as methods of using the same to treat patients in need of cardiac repair are disclosed.

公开(公告)号：AU2013255031C1

公开(公告)日：2018-12-06

申请号：AU2013255031

申请日：2013-04-30

Applicant: UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  NOSTRO MARIA CRISTINA ,  HOLTZINGER AUDREY ,  SARANGI FARIDA

IPC: C12N5/071 ,  C12N5/02 ,  C12N5/073 ,  C12P21/02

Abstract: Methods and compositions for producing NKX6-1+ pancreatic progenitor cells and/or insulin producing cells from an endodermal cell population, the method comprising contacting the endodermal cell population with an EGF component, a Nicotinamide component and/or a Noggin component, optionally a combination of at least one EGF component and at least one nicotinamide component to induce the differentiation of at least one endodermal cell into a NKX6-1+ pancreatic progenitor cell, the combination optionally further comprising at least one Noggin component.

公开(公告)号：SG11201506520TA

公开(公告)日：2015-09-29

申请号：SG11201506520T

申请日：2014-02-18

Applicant: UNIV HEALTH NETWORK ,  HOSPITAL FOR SICK CHILDREN

Inventor： KELLER GORDON ,  OGAWA SHINICHIRO ,  GHANEKAR ANAND ,  BEAR CHRISTINE ,  KAMATH BINITA M ,  OGAWA MINA ,  SURAPISITCHAT JAMES

IPC: C12N5/071 ,  A61K35/12 ,  A61K35/407 ,  A61P1/16 ,  C12N5/0735 ,  C12Q1/02

Abstract: Methods for producing hepatocyte and/or cholangiocyte lineage cells from pluripotent stem cells, the method comprising (a) specifying the extended nodal agonist treated induced endodermal cell population to obtain a cell population comprising hepatocyte and/or cholangiocyte progenitors by contacting the extended nodal agonist treated induced endodermal cell population with specification media comprising a FGF agonist and a BMP4 agonist and/or active conjugates and/or fragments thereof; (b) inducing maturation, and optionally further lineage specification and/or expansion of the hepatocyte and/or cholangiocyte progenitors of the cell population to obtain a population comprising hepatocyte lineage cells such as hepatoblasts, hepatocytes and/or cholangiocytes, the inducing maturation step comprising generating aggregates of the cell population. Optionally, the method also comprises activating the cAMP pathway within the aggregates and forming co-aggregates.

公开(公告)号：CA2856322A1

公开(公告)日：2013-05-30

申请号：CA2856322

申请日：2012-11-21

Applicant: UNIV HEALTH NETWORK ,  SUNNYBROOK RES INST

Inventor： KELLER GORDON ,  ZUNIGA-PFLUCKER JUAN CARLOS ,  KENNEDY MARION J ,  STURGEON CHRISTOPHER MICHAEL ,  DITADI ANDREA ,  AWONG GENEVE SHEANDRA

IPC: C12N5/0789 ,  C12N5/071 ,  C12Q1/24

Abstract: There is described herein a method of enriching a population of stem cells for hematopoietic progenitors. The method comprises inducing hematopoietic differentiation in a population of human embryonic stem cells or human induced pluripotent stem cells; sorting the population based on expression of CD43 and at least one of CD34, CD31 and CD144; and selecting a fraction that is at least one of CD34+CD43-, CD31+CD43- and CD144+CD43-. Also provided are populations of hematopoietic progenitors obtained by the methods described herein.

公开(公告)号：AU2022252839A1

公开(公告)日：2022-11-10

申请号：AU2022252839

申请日：2022-10-14

Applicant: UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  CRAFT APRIL M

IPC: C12N5/077 ,  A61K35/32 ,  A61P19/00 ,  C12N5/073 ,  C12Q1/02 ,  G01N33/48 ,  G01N33/50

Abstract: Abstract Methods and compositions for generating chondrocyte lineage cells and/or cartilage like tissue A method for generating chondrocytes and/or cartilage, optionally articular like non-hypertrophic chondrocyte cells and/or cartilage like tissue and/or hypertrophic chondrocyte like cells and/or cartilage like tissue, the method comprising: a. culturing a primitive streak-like mesoderm population, optionally a CD56+, PDGFRalpha+ KDR- primitive streak-like mesoderm population, with a paraxial mesoderm specifying cocktail comprising: i. a FGF agonist; ii. a BMP inhibitor; optionally Noggin, LDN-193189, Dorsomorphin; and iii. optionally one or more of a TGFbeta inhibitor, optionally SB431524; and a Wnt inhibitor, optionally DKK1, IWP2, or XAV939; to specify a paraxial mesoderm population expressing cell surface CD73, CD105 and/or PDGFR-beta; b. generating a chondrocyte precursor population comprising: i. culturing the paraxial mesoderm population expressing CD73, CD105 and/or PDGFR-beta at a high cell density optionally in serum free or serum containing media; ii. culturing the high cell density CD73+, CD105+ and/or PDGFRbeta+ paraxial mesoderm population with a TGFbeta3 agonist in serum free media to produce a high cell density Sox9+, collagen 2+ chondrocyte precursor population; and c. either i. culturing the high cell density Sox9+, collagen 2+ chondrocyte precursor population with the TGFbeta3 agonist for an extended period of time to produce an articular like non-hypertrophic chondrocyte cells and/or cartilage like tissue; or ii. culturing the high cell density Sox9+ collagen2+ chondrocyte precursor population with a BMP4 agonist for an extended period of time to produce a hypertrophic chondrocyte like cells and/or cartilage like tissue.

公开(公告)号：CA2976283C

公开(公告)日：2022-04-26

申请号：CA2976283

申请日：2016-02-17

Applicant: UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  PROTZE STEPHANIE

IPC: C12N5/077 ,  A61K35/34 ,  A61P9/00 ,  C12N5/071 ,  C12N5/0735 ,  C12N5/10

Abstract: Provided are methods for producing compositions comprising a population of cardiomyocytes enriched for or substantially devoid of sinoatrial node-like pacemaker cardiomyocytes (SANLCM) from human pluripotent stem cells (hPSCs), and methods of use thereof.