公开(公告)号：US11774449B2

公开(公告)日：2023-10-03

申请号：US16995559

申请日：2020-08-17

Applicant: University of Houston System

Inventor： Navin Varadarajan ,  Gabrielle Romain ,  Ivan Liadi ,  Victor Sendra ,  Badrinath Roysam

IPC: C12Q1/68 ,  G01N33/569 ,  G01N33/50 ,  C12Q1/6869

CPC classification number: G01N33/56966 ,  C12Q1/68 ,  C12Q1/6869 ,  G01N33/5005 ,  G01N2570/00

Abstract: Presented herein are methods of evaluating cellular activity by: placing a cell population on an area; assaying for a dynamic behavior of the cell population as a function of time; identifying cell(s) of interest based on the dynamic behavior; characterizing a molecular profile of the cell(s); and correlating the obtained information. The assayed dynamic behavior can include cellular activation, cellular inhibition, cellular interaction, protein expression, protein secretion, cellular proliferation, changes in cellular morphology, motility, cell death, cell cytotoxicity, cell lysis, and combinations thereof. Sensors associated with the area may be utilized to facilitate assaying. Molecular profiles of the cell(s) can then be characterized by various methods, such as DNA analysis, RNA analysis, and protein analysis. The dynamic behavior and molecular profile can then be correlated for various purposes, such as predicting clinical outcome of a treatment, screening cells, facilitating a treatment, diagnosing a disease, and monitoring cellular activity.

公开(公告)号：US10746736B2

公开(公告)日：2020-08-18

申请号：US15560018

申请日：2016-03-28

Applicant: UNIVERSITY OF HOUSTON SYSTEM

Inventor： Navin Varadarajan ,  Gabrielle Romain ,  Ivan Liadi ,  Victor Sendra ,  Badrinath Roysam

IPC: G01N33/00 ,  G01N33/569 ,  C12Q1/68 ,  G01N33/50 ,  C12Q1/6869

Abstract: Presented herein are methods of evaluating cellular activity by: placing a cell population on an area; assaying for a dynamic behavior of the cell population as a function of time; identifying cell(s) of interest based on the dynamic behavior; characterizing a molecular profile of the cell(s); and correlating the obtained information. The assayed dynamic behavior can include cellular activation, cellular inhibition, cellular interaction, protein expression, protein secretion, cellular proliferation, changes in cellular morphology, motility, cell death, cell cytotoxicity, cell lysis, and combinations thereof. Sensors associated with the area may be utilized to facilitate assaying. Molecular profiles of the cell(s) can then be characterized by various methods, such as DNA analysis, RNA analysis, and protein analysis. The dynamic behavior and molecular profile can then be correlated for various purposes, such as predicting clinical outcome of a treatment, screening cells, facilitating a treatment, diagnosing a disease, and monitoring cellular activity.

公开(公告)号：US20190054461A1

公开(公告)日：2019-02-21

申请号：US15764014

申请日：2016-09-30

Applicant: UNIVERSITY OF HOUSTON SYSTEM

Inventor： Navin Varadarajan

IPC: B01L3/00

Abstract: A multi-use combined micro and nanowell plate may provide nanowell arrays within the individual microwells of the plate. One or more microwells of the plate may provide an array of nanowells disposed at the bottom of the microwell. The combined micro and nanowell plate may be formed from a top frame with voids defining the microwells, and a bottom plate with voids defining the array of nanowells. When the top frame and the bottom plate are joined, the nanowell arrays may be aligned with the microwells to provide a combined micro/nanowell.