公开(公告)号：US20230233674A1

公开(公告)日：2023-07-27

申请号：US18100456

申请日：2023-01-23

Applicant: UNIVERSITY OF HOUSTON SYSTEM

Inventor： Navin Varadarajan ,  Ankita Leekha

IPC: A61K39/39 ,  A61P31/14 ,  A61P31/16 ,  A61K39/215

CPC classification number: A61K39/39 ,  A61P31/14 ,  A61P31/16 ,  A61K39/215 ,  A61K2039/543

Abstract: Described herein are compositions and methods for the reducing a risk of contracting a respiratory disease in a subject or reducing a risk of transmitting a respiratory disease from a first subject to a second subject. In some cases, a composition or method described herein can comprise a modulator (e.g., a pattern recognition receptor, such as a STING agonist, for instance cGAMP) In some cases, a composition or method described herein can comprise a liposome, which may be used to encapsulate one or more STING agonists. In some cases, a liposome of a composition or method described herein may comprise one or more antigens attached to, integrated into, or associated with a liposomal membrane of the liposome.

公开(公告)号：US20210018503A1

公开(公告)日：2021-01-21

申请号：US16995559

申请日：2020-08-17

Applicant: University of Houston System

Inventor： Navin Varadarajan ,  Gabrielle Romain ,  Ivan Liadi ,  Victor Sendra ,  Badrinath Roysam

IPC: G01N33/569 ,  C12Q1/68 ,  G01N33/50 ,  C12Q1/6869

Abstract: Presented herein are methods of evaluating cellular activity by: placing a cell population on an area; assaying for a dynamic behavior of the cell population as a function of time; identifying cell(s) of interest based on the dynamic behavior; characterizing a molecular profile of the cell(s); and correlating the obtained information. The assayed dynamic behavior can include cellular activation, cellular inhibition, cellular interaction, protein expression, protein secretion, cellular proliferation, changes in cellular morphology, motility, cell death, cell cytotoxicity, cell lysis, and combinations thereof. Sensors associated with the area may be utilized to facilitate assaying. Molecular profiles of the cell(s) can then be characterized by various methods, such as DNA analysis, RNA analysis, and protein analysis. The dynamic behavior and molecular profile can then be correlated for various purposes, such as predicting clinical outcome of a treatment, screening cells, facilitating a treatment, diagnosing a disease, and monitoring cellular activity.

公开(公告)号：US20230270845A1

公开(公告)日：2023-08-31

申请号：US18017659

申请日：2021-07-22

Applicant: UNIVERSITY OF HOUSTON SYSTEM

Inventor： Navin Varadarajan ,  Xinli Liu

IPC: A61K39/215 ,  A61K39/39 ,  A61P31/14

CPC classification number: A61K39/215 ,  A61K39/39 ,  A61P31/14 ,  A61K2039/55555

Abstract: Described herein are compositions and methods for the prevention of pathogens such as conditions in an animal (e.g., a subject). In some cases, a composition or method described herein can comprise a liposome, which may be used to encapsulate one or more STING agonists. In some cases, a liposome of a composition or method described herein may comprise one or more antigens attached to, integrated into, or associated with a liposomal membrane of the liposome.

公开(公告)号：US20180112210A1

公开(公告)日：2018-04-26

申请号：US15571220

申请日：2016-05-23

Applicant: UNIVERSITY OF HOUSTON SYSTEM

Inventor： Navin Varadarajan ,  Jay R. Adolacion ,  Richard C. Willson

IPC: C12N15/10

CPC classification number: C12N15/1062 ,  C12N15/10 ,  C12N15/1037 ,  C12Q1/68 ,  C40B40/10 ,  G01N33/543 ,  G01N33/68 ,  G01N33/6845

Abstract: In some embodiments, the present disclosure pertains to method of screening a biological sample for a plurality of diseases. In some embodiments, such a method comprises obtaining a biological sample from a subject in need thereof. In some embodiments, the biological sample comprises a plurality of biomarkers. In some embodiments, each of the plurality of biomarkers is specific for at least one disease. In some embodiments, the method comprises contacting the biological sample with a display library of peptides. In some embodiments, each peptide in the library may have a unique amino acid sequence. In some embodiments, each of the peptides is physically linked to a nucleic acid sequence that identifies of encodes the peptide. In some embodiments, at least one of the peptides is capable of binding to at least one of the biomarkers in the biological sample. In some embodiments, the method comprises separating the bound peptide particles from the unbound peptide particle. In some embodiments, the method comprises eluting the bound peptide particles from the bound state. In some embodiments, the method comprises determining the identity of the nucleic acid sequences physically linked to the bound peptide particles. In some embodiments, the method comprises comparing the sequences thus obtained to a database of sequences representing a plurality of biomarkers of diseases.

公开(公告)号：US20240175870A1

公开(公告)日：2024-05-30

申请号：US18235751

申请日：2023-08-18

Applicant: University of Houston System

Inventor： Navin Varadarajan ,  Gabrielle Romain ,  Ivan Liadi ,  Victor G. Sendra ,  Badrinath Roysam

IPC: G01N33/569 ,  C12Q1/68 ,  C12Q1/6869 ,  G01N33/50

CPC classification number: G01N33/56966 ,  C12Q1/68 ,  C12Q1/6869 ,  G01N33/5005 ,  G01N2570/00

Abstract: Presented herein are methods of evaluating cellular activity by: placing a cell population on an area; assaying for a dynamic behavior of the cell population as a function of time; identifying cell(s) of interest based on the dynamic behavior; characterizing a molecular profile of the cell(s); and correlating the obtained information. The assayed dynamic behavior can include cellular activation, cellular inhibition, cellular interaction, protein expression, protein secretion, cellular proliferation, changes in cellular morphology, motility, cell death, cell cytotoxicity, cell lysis, and combinations thereof. Sensors associated with the area may be utilized to facilitate assaying. Molecular profiles of the cell(s) can then be characterized by various methods, such as DNA analysis, RNA analysis, and protein analysis. The dynamic behavior and molecular profile can then be correlated for various purposes, such as predicting clinical outcome of a treatment, screening cells, facilitating a treatment, diagnosing a disease, and monitoring cellular activity.

公开(公告)号：US20220195389A1

公开(公告)日：2022-06-23

申请号：US17604714

申请日：2020-04-17

Applicant: University of Houston System

Inventor： Navin Varadarajan ,  Irfan Naseem Bandey ,  Melissa Martinez Paniagua

IPC: C12N5/0783 ,  C07K14/725 ,  C07K16/28 ,  C12N9/12

Abstract: Embodiments of the present disclosure pertain to methods of modifying immune cells by increasing the AMP-activated protein kinase (AMPK) activity of the immune cells in order to produce modified immune cells with enhanced longevity. In some embodiments, the methods of the present disclosure include one or more of the following steps: (1) obtaining immune cells from a subject; (2) increasing the AMPK activity of the immune cells; (3) expanding the modified immune cells; (4) introducing the modified immune cells to a subject; and (5) treating a disease in the subject. In further embodiments, the methods of the present disclosure include an in vivo method of increasing the AMPK activity of immune cells in a subject in order to treat a disease in the subject. Additional embodiments of the present disclosure pertain to the modified immune cells.

公开(公告)号：US20230277667A1

公开(公告)日：2023-09-07

申请号：US17984169

申请日：2022-11-09

Applicant: University of Houston System

Inventor： Navin Varadarajan ,  Irfan Naseem Bandey

IPC: A61K39/00 ,  C12N9/50 ,  A61K39/395 ,  A61K31/65 ,  A61K31/704 ,  A61K31/5377 ,  A61K31/436 ,  A61K31/517 ,  A61K31/4545 ,  A61P35/00

CPC classification number: A61K39/4631 ,  A61K31/436 ,  A61K31/4545 ,  A61K31/517 ,  A61K31/5377 ,  A61K31/65 ,  A61K31/704 ,  A61K39/3955 ,  A61K39/4611 ,  A61K39/4637 ,  A61P35/00 ,  C12N9/50 ,  C12Y304/24034

Abstract: Provided herein are compositions containing genetically modified immune cells that express a ECM degrading enzyme, such as a matrix metallopeptidase. Overexpression of mature matrix metallopeptidase improves the tumor infiltrating capabilities of immune cells against solid tumors, including T-cells, tumor infiltrating lymphocytes, CAR macrophages, and NK cells (with and without a chimeric antigen receptor). Methods include administering CAR T or NK cells modified to express mature matrix metallopeptidase 8 to a subject with a solid tumor to improve the efficacy of the CAR T/NK cell therapy.

公开(公告)号：US20180243340A1

公开(公告)日：2018-08-30

申请号：US15753662

申请日：2016-08-23

Applicant: UNIVERSITY OF HOUSTON SYSTEM

Inventor： Navin Varadarajan ,  Gabrielle Romain ,  Laurence Cooper ,  Harjcct Singh

IPC: A61K35/17 ,  C12N5/0783 ,  G01N33/50 ,  C07K19/00

CPC classification number: A61K35/17 ,  A61P35/00 ,  C07K19/00 ,  C07K2319/03 ,  C12N5/0636 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/53 ,  C12N2501/599 ,  G01N33/502

Abstract: In some embodiments, the present disclosure pertains to a method of enhancing chimeric antigen receptor expressing T cell function. In some embodiments, the method comprises activating the chimeric antigen receptor expressing T cells. In some embodiments, the method further comprises determining the differential expression of at least one molecule on the chimeric antigen receptor T cells. In some embodiments, the method comprises targeting the at least one molecule. In some embodiments, the present disclosure pertains to a method of treating a tumor in a subject in need thereof. In some embodiments, the method comprises administering to the subject an infusion of chimeric antigen receptor expressing T cells. In some embodiments, the method further comprises determining the differential expression of at least one molecule on the chimeric antigen receptor T cells. In some embodiments, the method comprises targeting the at least one molecule, wherein the molecule is differentially expressed upon activation of the chimeric antigen receptor expressing T cells.

公开(公告)号：US20180217146A1

公开(公告)日：2018-08-02

申请号：US15560018

申请日：2016-03-28

Applicant: UNIVERSITY OF HOUSTON SYSTEM

Inventor： Navin Varadarajan ,  Gabrielle Romain ,  Ivan Liadi ,  Victor Sendra ,  Badrinath Roysam

IPC: G01N33/569 ,  C12Q1/6869

CPC classification number: G01N33/56966 ,  C12Q1/68 ,  C12Q1/6869 ,  G01N33/5005 ,  G01N2570/00

Abstract: Presented herein are methods of evaluating cellular activity by: placing a cell population on an area; assaying for a dynamic behavior of the cell population as a function of time; identifying cell(s) of interest based on the dynamic behavior; characterizing a molecular profile of the cell(s); and correlating the obtained information. The assayed dynamic behavior can include cellular activation, cellular inhibition, cellular interaction, protein expression, protein secretion, cellular proliferation, changes in cellular morphology, motility, cell death, cell cytotoxicity, cell lysis, and combinations thereof. Sensors associated with the area may be utilized to facilitate assaying. Molecular profiles of the cell(s) can then be characterized by various methods, such as DNA analysis, RNA analysis, and protein analysis. The dynamic behavior and molecular profile can then be correlated for various purposes, such as predicting clinical outcome of a treatment, screening cells, facilitating a treatment, diagnosing a disease, and monitoring cellular activity.

公开(公告)号：US20180140686A1

公开(公告)日：2018-05-24

申请号：US15573013

申请日：2016-05-20

Applicant: University of Houston System

Inventor： Navin Varadarajan ,  Irfan Naseem Bandey

IPC: A61K38/50 ,  A61P35/02 ,  G01N33/574 ,  C07K16/28 ,  C07K16/30 ,  A61K47/69

CPC classification number: A61K38/50 ,  A61K47/6929 ,  A61P35/02 ,  C07K16/2896 ,  C07K16/30 ,  C07K2317/52 ,  C07K2319/035 ,  C07K2319/30 ,  C07K2319/33 ,  C12Y305/04004 ,  G01N33/57484

Abstract: In some embodiments the present disclosure pertains to a method of activating an anti-tumor immune response for the treatment of a cancer. In some embodiments, such a method comprises detecting CD26 expression in a subject in need thereof. In some embodiments, the method comprises administering to the subject a therapeutically effective amount of a composition comprising adenosine deaminase. In some embodiments, the adenosine deaminase stimulates T cell proliferation and activates maturation of macrophages or dendritic cells. In some embodiments, the present disclosure pertains to a method for targeted reduction of adenosine or deoxyadenosine in a tumor microenvironment of a solid tumor.