公开(公告)号：WO2004108939A3

公开(公告)日：2004-12-16

申请号：PCT/IB2004/002409

申请日：2004-06-10

Applicant: UNIVERSITY OF SASKATCHEWAN ,  TIKOO, Suresh, K. ,  XING, Li

Inventor： TIKOO, Suresh, K. ,  XING, Li

IPC: C12N15/86

Abstract: The present invention relates to the identification of bovine adenovirus sequence(s) essential for encapsidation and El transcriptional control regions. The present invention provides adenovirus expression systems, host cells and compositions comprising adenovirus vectors which comprise one or more BAV sequence(s) essential for encapsidation, as well as helper virus which express BAV sequences essential for encapsidation. The present invention also provides helper vectors comprising a BAV sequence essential for encapsidation which is used in a helper virus for propagating recombinant adenovirus. The present invention also provides adenovirus expression systems, host cells and compositions comprising adenovirus vectors which comprise modifications in BAV E1 transcriptional control regions. The present invention also provides methods for making adenovirus vectors comprising BAV sequence(s) essential for encapsidation as well as modifications in BAV E1 transcriptional control regions.

公开(公告)号：WO2004108755A2

公开(公告)日：2004-12-16

申请号：PCT/IB2004/002532

申请日：2004-06-10

Applicant: UNIVERSITY OF SASKATCHEWAN ,  TIKOO, Suresh, K. ,  ZAKHARTCHOUK, Alexandre, N. ,  BABIUK, Lorne, A. ,  KAUSHIK, Radhey, S. ,  GRIEBEL, Philip, J.

Inventor： TIKOO, Suresh, K. ,  ZAKHARTCHOUK, Alexandre, N. ,  BABIUK, Lorne, A. ,  KAUSHIK, Radhey, S. ,  GRIEBEL, Philip, J.

IPC: C07K14/075

CPC classification number: C07K14/005 ,  C07K2319/33 ,  C12N2710/10322 ,  C12N2810/405 ,  C12N2810/859

Abstract: The present invention relates to chimeric adenovirus capsid proteins comprising a part of or all of an adenovirus capsid protein and a binding partner of a cell-surface binding site on a cell present in gut associated lymphoid tissues (GALT) of a mammal, wherein the chimeric adenovirus capsid protein is capable of binding the cell present in gut associated lymphoid tissues (GALT). In some examples, the adenovirus capsid protein is located on the surface of the adenovirus capsid. The present invention provides adenovirus capsids comprising a chimeric capsid protein. The present invention also provides complexes comprising a chimeric adenovirus capsid protein bound to a cell in GALT. The present invention also provides encapsidation systems and vectors, in particular adenovirus vectors, capable of expressing a chimeric adenovirus capsid protein encompassed within the invention. The present invention also provides viral particles, host cells and compositions comprising such vectors, in particular for use in vaccines, methods of eliciting an immune response and methods for targeted delivery of heterologous proteins, such as antigens, to target cells.

Abstract translation: 本发明涉及包含哺乳动物肠道相关淋巴组织（GALT）中存在的细胞上的一部分或全部腺病毒衣壳蛋白和细胞表面结合位点的结合配偶体的嵌合腺病毒衣壳蛋白，其中嵌合 腺病毒衣壳蛋白能够结合存在于肠道相关淋巴组织（GALT）中的细胞。 在一些实例中，腺病毒衣壳蛋白位于腺病毒衣壳的表面上。 本发明提供了包含嵌合衣壳蛋白的腺病毒衣壳。 本发明还提供了包含与GALT中的细胞结合的嵌合腺病毒衣壳蛋白的复合物。 本发明还提供能够表达包含在本发明内的嵌合腺病毒衣壳蛋白的包衣系统和载体，特别是腺病毒载体。 本发明还提供病毒颗粒，宿主细胞和包含这种载体的组合物，特别是用于疫苗，引发免疫应答的方法和用于将异源蛋白质例如抗原靶向递送至靶细胞的方法。

公开(公告)号：WO2004079001A1

公开(公告)日：2004-09-16

申请号：PCT/CA2004/000332

申请日：2004-03-04

Applicant: UNIVERSITY OF SASKATCHEWAN TECHNOLOGIES INC. ,  LEE, Jeremy, S. ,  KRAATZ, Heinz-Bernhard ,  LI, Chen-Zhong ,  LONG, Yi-Tao

Inventor： LEE, Jeremy, S. ,  KRAATZ, Heinz-Bernhard ,  LI, Chen-Zhong ,  LONG, Yi-Tao

IPC: C12Q1/68

CPC classification number: C12Q1/6825 ,  B82Y15/00 ,  G01N27/3277

Abstract: In one aspect, the invention provides methods and apparatus for detecting a mismatch in a nucleic acid duplex by measuring the impedence of a nucleic acid layer on an electrode, for example by AC impedance spectroscopy.

Abstract translation: 一方面，本发明提供了通过例如通过AC阻抗谱法测量电极上的核酸层的阻抗来检测核酸双链体错配的方法和装置。

公开(公告)号：WO2004044207A9

公开(公告)日：2004-05-27

申请号：PCT/CA2003/001754

申请日：2003-11-14

Applicant: UNIVERSITY OF SASKATCHEWAN TECHNOLOGIES INC. ,  ROBERTSON, Albert, James ,  GUSTA, Lawrence, Victor ,  WU, Guohai

Inventor： ROBERTSON, Albert, James ,  GUSTA, Lawrence, Victor ,  WU, Guohai

IPC: C12N15/82

Abstract: Plant stresses such as pest infestations, disease, drought, flood, and excessive temperatures can lead to significant losses of crops each year. There is a continuing need to develop novel plant varieties that are less susceptible to damage or loss by such stresses. The present invention provides for the isolation, characterization and use of an entirely novel class of plant genes, generally designated ROB5 . Transgenic plants expressing ROB5 can show a dramatic improvement in their capacity to tolerate a variety of stress conditions. Moreover, ROB5 expression can further lead to marked increases in plant growth rates and plant vigor. The present invention encompasses all such ROB5 genes and peptides encoded thereby, plants expressing corresponding ROB5 constructs, and plant products thereof.

公开(公告)号：WO2004009824A2

公开(公告)日：2004-01-29

申请号：PCT/IB2003/003724

申请日：2003-07-18

Applicant: UNIVERSITY OF SASKATCHEWAN ,  TIKOO, Suresh, K.

Inventor： TIKOO, Suresh, K.

IPC: C12N15/86

CPC classification number: C12N15/86 ,  A61K48/00 ,  A61K2039/5256 ,  C12N2710/10343 ,  C12N2830/30 ,  C12N2830/32

Abstract: The present invention provides porcine adenovirus sequence essential for encapsidation and provides adenovirus vectors comprising such sequences. The present invention provides host cells and composition comprising adenovirus vectors comprising porcine adenovirus sequence essential for encapsidation as well as methods for making and using such adenovirus vectors. The present invention discloses porcine adenovirus sequence for E1 transcriptional control and provides porcine adenovirus vectors comprising a modificatin(s) in the E1 transcriptional control region.

Abstract translation: 本发明提供壳衣化所必需的猪腺病毒序列，并提供包含此类序列的腺病毒载体。 本发明提供了包含腺病毒载体的宿主细胞和组合物，所述腺病毒载体包含壳体必需的猪腺病毒序列以及制备和使用此类腺病毒载体的方法。 本发明公开了用于E1转录控制的猪腺病毒序列，并提供了在E1转录控制区中包含修饰蛋白的猪腺病毒载体。

公开(公告)号：WO2003091437A1

公开(公告)日：2003-11-06

申请号：PCT/CA2003/000587

申请日：2003-04-24

Applicant: UNIVERSITY OF SASKATCHEWAN

Inventor： POTTER, Andrew, A. ,  PEREZ-CASAL, Jose ,  FONTAINE, Michael ,  SONG, Xinming

IPC: C12N15/31

CPC classification number: C07K14/315 ,  A61K39/00 ,  C07K2319/02

Abstract: The CAMP factor gene of Streptococcus uberis ( S. uberis ) is described, as well as the recombinant production of CAMP factor therefrom. Also disclosed are chimeric CAMP factor constructs, including CAMP factor epitopes from more than one bacterial species. The CAMP factors and chimeras including the same can be used in immunogenic compositions for the prevention and treatment of bacterial infections.

Abstract translation: 描述了乳房链球菌（乳房链球菌）的CAMP因子基因，以及从其重组产生CAMP因子。 还公开了嵌合CAMP因子构建体，包括来自多于一种细菌种的CAMP因子表位。 包括其的CAMP因子和嵌合体可用于免疫原性组合物中用于预防和治疗细菌感染。

公开(公告)号：WO2003040305A3

公开(公告)日：2003-05-15

申请号：PCT/IB2002/005829

申请日：2002-09-24

Applicant: UNIVERSITY OF SASKATCHEWAN ,  TIKOO, Suresh, K.

Inventor： TIKOO, Suresh, K.

IPC: C12N15/86

Abstract: The present invention relates to the characterization of the porcine adenovirus E1 and E4 regions. The complete nucleotide sequence of the genome of porcine adenovirus type 3 (PAV-3), providing the characterization of the PAV3 E1 and E4 region, is described herein. Methods for construction of infectious PAV genomes by homologous recombination in procaryotic cells are provided. Recombinant PAV viruses are obtained by transfection of mammalian cells with recombinant PAV genomes. The PAV-3 genome can be used as a vector for the expression of heterologous nucleotide sequences, for example, for the preparation and administration of subunit vaccines to swine or other mammals.

公开(公告)号：WO2001092547A3

公开(公告)日：2001-12-06

申请号：PCT/CA2001/000798

申请日：2001-05-31

Applicant: UNIVERSITY OF SASKATCHEWAN ,  TIKOO, Suresh, K.

Inventor： TIKOO, Suresh, K.

IPC: C12N15/86

Abstract: The present invention provides modified bovine adenoviruses comprising a modification in a capsid protein wherein said protein is associated with adenovirus tropism and wherein said modification is associated with altered tropism. The present invention provides adenovirus vectors and host cells comprising such vectors. The present invention also provides methods of making and using such adenoviruses.

公开(公告)号：WO1996041879A1

公开(公告)日：1996-12-27

申请号：PCT/CA1996000377

申请日：1996-06-07

Applicant: UNIVERSITY OF SASKATCHEWAN

Inventor： UNIVERSITY OF SASKATCHEWAN ,  JIANG, Min ,  POTTER, Andrew, A. ,  MACLACHLAN, Philip, Ronald

IPC: C12N15/31

CPC classification number: C07K14/315 ,  A61K39/00 ,  Y10S930/29

Abstract: The CAMP factor gen of Streptococcus uberis (S. uberis) is described, as well as the recombinant production of CAMP factor therefrom. CAMP factors can be used in vaccine compositions for the prevention and treatment of bacterial infections.

Abstract translation: 描述了乳房链球菌（乳房链球菌）的CAMP因子基因，以及从其重组产生CAMP因子。 CAMP因子可用于预防和治疗细菌感染的疫苗组合物中。

公开(公告)号：WO1995033365A1

公开(公告)日：1995-12-14

申请号：PCT/CA1995000324

申请日：1995-06-01

Applicant: UNIVERSITY OF SASKATCHEWAN ,  ZYLA, Loyd, E. ,  KUSHWAHA, Lal, R. ,  REED, William, B.

Inventor： UNIVERSITY OF SASKATCHEWAN

IPC: A01D45/00

CPC classification number: A01D45/22 ,  A01D45/003 ,  A01D57/02

Abstract: A crop harvester has bristle guards (39) mounted on the cutter bar (14) as crop lifters and to capture fruit lost by dehiscence during cutting. Each bristle guard is a set of laterally oriented bristles (44). Dehisced fruit is swept into the header by a pickup reel (16) having bristle bats (74). The bristles (76) are supported on the leading side by a strip (78) of flexible belting. The belting protects the bristle bats against being cut by the cutter bar. The crops are supported against being pushed forward by the bristle bats (74) of the reel. The reel diameter is reduced to about one-half normal and the reel index is increased to provide an aggressive reel operation, ensuring that plants are properly engaged in the bristle guards for cutting.

Abstract translation: 作物收割机具有作为作物升降器安装在切割杆（14）上的刷毛防护件（39），并且在切割期间捕获由开裂损失的水果。 每个刷毛防护罩是一组横向定向的刷毛（44）。 旋开的水果被具有刷毛蝙蝠（74）的拾取卷轴（16）扫入头部。 刷毛（76）由柔性带的带（78）支撑在前侧。 皮带保护鬃毛蝙蝠不被切割杆切割。 作物被支撑以被卷轴的刷毛蝙蝠（74）向前推动。 卷轴直径减小到正常的大约一半，并且卷轴索引增加以提供积极的卷轴操作，确保植物适当地接合在刷毛防护件中用于切割。