Abstract:
A method for finding L internal reference vectors for classification of L chromosomes or portions of chromosomes of a cell, the L chromosomes or portions of chromosomes being painted with K different fluorophores or combinations thereof, wherein K basic chromosomes or portions of chromosomes of the L chromosomes or portions of chromosomes are each painted with only one of the K different fluorophores, whereas the other L-K of the L chromosomes or portions of chromosomes are each painted with a different combination of the K different fluorophores, the method comprising the steps of (a) using a multi-band collection device for measuring a first vector for each pixel of each of the L chromosomes or portions of chromosomes; (b) identifying pixels belonging to each of the K basic chromosomes or portions of chromosomes and defining the pixels as basic pixels, so as to obtain K basic classes of basic pixels; (c) using at least one basic pixel from each of the K basic classes for obtaining K basic vectors, the K basic vectors being K internal reference vectors; (d) using the K basic vectors for identifying pixels belonging to the other L-K chromosomes or portions of chromosomes; and (e) using the pixels belonging to the other L-K chromosomes or portions of chromosomes for calculating the other L-K internal reference vectors, thereby finding all of the L internal reference vectors. A method for classification of L chromosomes or portions of chromosomes of a cell similarly painted using the above method for finding L internal reference vectors, and using the L reference vectors for classification of each of the pixels into one of L classification classes. And, images presenting color chromosomes.
Abstract:
PROBLEM TO BE SOLVED: To provide a dynamic light scattering measuring device using a phase modulation type interferometry capable of clarifying optical path length dependency of a multiple scattering spectrum, and thereby measuring a dynamic characteristic of a medium with excellent accuracy based on scattered light from the high-concentration medium. SOLUTION: This device is equipped with an optical coupler 5 for dividing light from a low coherence light source 2, a condensing lens 10 for irradiating the sample medium 9 with one light divided by the optical coupler 5, phase modulators 7, 8 for modulating the phase of the other light divided by the optical coupler 5, a spectrum measuring device 12 for measuring the spectrum of interference light between phase-modulated reference light and scattered light emitted from the sample medium 9, and an analysis means for performing dynamic light scattering measurement of particles based on a 1-st order spectrum appearing in an interference light spectrum measured by the spectrum measuring device 12 and corresponding to a fundamental frequency of a phase modulation signal or a higher-order spectrum corresponding to a frequency in double, triple or the like. As for the optical path length s in the sample medium, the quantity s/L standardized by the mean free path of the particles is set to be 3 or less. COPYRIGHT: (C)2005,JPO&NCIPI
Abstract:
PROBLEM TO BE SOLVED: To provide a chromosome display device. SOLUTION: The chromosome display device consists of a multifrequency band device (a) caught by algorithm for presenting the image of chromosomes or chromosome parts of cells colored by respectively different emission fluorophores or a combination of them to a visual presentation device by peculiar colors respectively different with respect to the chromosome parts of cells in such a state that one of different peculiar colors is applied to each of the chromosome parts of cells and the visual presentation device (b) for displaying the chromosome parts of cells.