公开(公告)号：WO2004013610A2

公开(公告)日：2004-02-12

申请号：PCT/US2003/024780

申请日：2003-08-01

Applicant: SENSOR TECHNOLOGIES LLC

Inventor： WOLF, David, E. ,  BULSECO, Dylan, A.

IPC: G01N

CPC classification number: G01N21/6428 ,  G01J3/4406 ,  G01J3/457 ,  G01N21/645 ,  G01N33/582 ,  G01N2021/6417 ,  G01N2021/6482 ,  G01N2021/6484

Abstract: The invention features a method of assaying for the interaction of a probe and an unknown target, said method including a) exciting a sample with radiation, the sample including at least one unknown target, at least one probe, and at least one fluorescent tag, b) measuring the fluorescence from a subvolume of the sample, and c) analyzing the fluctuations of the fluorescence.

Abstract translation: 本发明的特征在于测定探针和未知靶的相互作用的方法，所述方法包括a）用辐射激发样品，所述样品包括至少一个未知靶，至少一个探针和至少一个荧光标记， b）测量来自样品的子体积的荧光，和c）分析荧光的波动。

公开(公告)号：WO2003010523A1

公开(公告)日：2003-02-06

申请号：PCT/FR2002/002696

申请日：2002-07-26

Applicant: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) ,  RIGNEAULT, Hervé ,  LENNE, Pierre-François

Inventor： RIGNEAULT, Hervé ,  LENNE, Pierre-François

IPC: G01N21/64

CPC classification number: G01N21/6458 ,  G01J3/4406 ,  G01J3/457 ,  G01N15/0211 ,  G01N21/6408 ,  G02B21/0056 ,  G02B21/0076

Abstract: L'invention concerne un dispositif et un procédé de mesure d'un échantillon (2) par spectroscopie par corrélation. Le dispositif comprend un microscope confocal (1) comportant un système optique de focalisation (4) dont le champ (7) définit un volume de collection (9). Une source de lumière (10) produit un faisceau d'excitation (8) qui est envoyé vers des moyens (11) aptes à le diriger sur l'échantillon (2) au travers du microscope. Le dispositif de mesure comporte également des moyens de détection (14) de l'intensité du flux lumineux (13) produit par l'interaction du faisceau d'excitation (8) sur l'échantillon (2) et collecté par le microscope et des moyens de traitement (15) du signal produit par lesdits moyens de détection (14). Une structure photonique (24) augmentant le flux lumineux (13) collecté, est placée au foyer (7) du système optique de focalisation (4) du microscope ou conjuguée de ce foyer (7) par un élément optique (29), pour former des franges d'interférence (25) dans le volume de collection (9).

Abstract translation: 本发明涉及通过相关光谱测量样品（2）的装置和方法。 该装置包括共聚焦显微镜（1），其包括聚焦光学系统（4），其中场（7）限定收集容积（9）。 光源（10）产生激发光束（8），其被反射到装置（11），用于通过显微镜将其引导到样品（2）上。 测量装置还包括用于检测（14）通过样品上的激发束（8）与显微镜收集的相互作用产生的光通量（13）的强度的装置，以及用于处理（15）由 检测装置（14）。 增加收集的光通量（13）的光子结构（24）被布置在显微镜聚焦光学系统（4）的焦点（7）处或通过光学元件（29）与所述焦点（7）组合以形成 收集容积（9）中的干涉条带（25）。

公开(公告)号：WO01065219A1

公开(公告)日：2001-09-07

申请号：PCT/GB2001/000711

申请日：2001-02-20

IPC: G01J3/28 ,  G01J3/447 ,  G01J3/457 ,  G01N21/35

CPC classification number: G01N21/3504 ,  G01J3/447 ,  G01J3/457 ,  G01J2003/2866

Abstract: A spectrometer for determining the concentration of a substance within a sample comprises a. a radiation source (11) for supplying radiation to the sample to be measured; b. a filter (15) for filtering radiation transmitted by the sample, the filter having a number of pass bands at wavelengths corresponding to absorption peaks in the absorption spectrum of the sample to be detected, the filter being responsive to an applied signal to modulate the wavelengths of the pass bands; and, c. a detector (16) for detecting the filtered radiation, the detector being responsive to the applied signal to determine the relative intensities of the maxima and minima in the absorption spectra of the substance, characterized in that the radiation source is selectively activated in response to the applied signal.

Abstract translation: 用于确定样品中物质浓度的光谱仪包括a。 辐射源（11），用于向被测样品供给辐射; 湾 滤波器（15），用于滤波由样品透射的辐射，滤光器具有多个通过带，其波长对应于待检测样品的吸收光谱中的吸收峰，该滤波器响应所施加的信号以调制波长 的通带; 和，c。 检测器（16），用于检测经滤波的辐射，所述检测器响应所施加的信号以确定所述物质的吸收光谱中的最大值和最小值的相对强度，其特征在于，所述辐射源根据 应用信号。

公开(公告)号：WO01059416A1

公开(公告)日：2001-08-16

申请号：PCT/EP2001/001431

申请日：2001-02-09

IPC: G01J3/28 ,  G01J3/44 ,  G01J3/457 ,  G01N21/64

CPC classification number: G01N21/6458 ,  G01J3/4406 ,  G01J3/457 ,  G01J2003/2866 ,  G01N21/64 ,  G01N21/6408 ,  G01N2021/6421 ,  G01N2021/6463

Abstract: The present invention relates to a method for characterizing samples having fluorescent particles, comprising the steps of: (a) monitoring intensity fluctuations of fluorescence emitted by the particles in at least one measurement volume by detecting sequences of photon counts by at least one photon detector, (b) determining from the sequences of photon counts intermediate statistical data comprising at least two probability functions, P 1(n1), P 2(n2),..., of the number of photon counts, n1, n2,..., detected in different sets of counting time intervals, (c) determining from said intermediate statistical data a distribution of particles as a function of at least two arguments, wherein one argument is a specific brightness of the particles, or a measure thereof, and another argument is a diffusion coefficient of the particles, or a measure thereof.

Abstract translation: 本发明涉及一种用于表征具有荧光颗粒的样品的方法，包括以下步骤：（a）通过至少一个光子检测器检测光子计数序列来监测由至少一个测量体积中的颗粒发射的荧光的强度波动， （b）根据光子计数的序列确定包括至少两个概率函数的中间统计数据，P 1（n 1），P 2（n 2），...， 在不同的计数时间间隔集中检测的光子计数数目n1，n2，...，（c）根据所述中间统计数据确定作为至少两个参数的函数的粒子分布，其中一个参数是 颗粒的特定亮度或其度量，另一个参数是颗粒的扩散系数或其度量。

公开(公告)号：WO00006774A1

公开(公告)日：2000-02-10

申请号：PCT/US1999/016629

申请日：1999-07-23

IPC: G01N33/53 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/42 ,  C12Q1/68 ,  G01J3/28 ,  G01J3/457 ,  G01N21/78 ,  G01N33/50 ,  G01N33/533 ,  G01N33/569 ,  G01N33/58

CPC classification number: G01J3/457 ,  G01J3/2823 ,  G01J2003/2866 ,  G01N33/5005 ,  G01N33/56966

Abstract: A method of in situ analysis of a biological sample comprising the steps of (a) staining the biological sample with N stains of which a first stain is selected from the group consisting of a first immunohistochemical stain, a first histological stain and a first DNA ploidy stain, and a second stain is selected from the group consisting of a second immunohistochemical stain, a second histological stain and a second DNA ploidy stain, with provisions that N is an integer greater than three and further that (i) if the first stain is the first immunohistochemical stain then the second stain is either the second histological stain or the second DNA ploidy stain; (ii) if the first stain is the first histological stain then the second stain is either the second immunohistochemical stain or the second DNA ploidy stain; whereas (iii) if the first stain is the first DNA ploidy stain then the second stain is either the second immunohistochemical stain or the second histological stain; and (b) using a spectral data collection device for collecting spectral data from the biological sample, the spectral data collection device and the N stains are selected such that a spectral component associated with each of the N stains is collectable.

Abstract translation: 一种生物样品的原位分析方法，包括以下步骤：（a）染色具有N染色的生物样品，其中第一染色选自第一免疫组织化学染色，第一组织染色和第一DNA倍体 染色，第二染色选自第二免疫组织化学染色，第二组织学染色和第二DNA倍性染色，其规定N为大于3的整数，并且（i）如果第一染色是 第一次免疫组织化学染色，第二次染色是第二次组织染色或第二次DNA倍体染色; （ii）如果第一染色是第一组织染色，则第二染色是第二免疫组织化学染色或第二DNA倍体染色; 而（iii）如果第一个染色是第一个DNA倍性染色，则第二次染色是第二次免疫组化染色或第二次组织染色; 和（b）使用用于收集来自生物样品的光谱数据的光谱数据采集装置，选择光谱数据采集装置和N染色，使得与每个N染色体相关联的光谱分量是可收集的。

公开(公告)号：US11953427B2

公开(公告)日：2024-04-09

申请号：US15227876

申请日：2016-08-03

Applicant: SpectraSensors, Inc.

Inventor： Alfred Feitisch ,  Xiang Liu ,  Kevin Ludlum ,  Mathias Schrempel

IPC: G01N21/27 ,  G01J3/02 ,  G01J3/28 ,  G01J3/42 ,  G01J3/457 ,  G01N21/39 ,  G01N21/21 ,  G01N21/35 ,  G01N21/3504 ,  G01N21/359

CPC classification number: G01N21/274 ,  G01J3/0297 ,  G01J3/28 ,  G01J3/42 ,  G01J3/457 ,  G01N21/39 ,  G01J2003/423 ,  G01N21/211 ,  G01N21/35 ,  G01N21/3504 ,  G01N21/359 ,  G01N2021/3595 ,  G01N2021/399

Abstract: Frequency registration deviations occurring during a scan of a frequency or wavelength range by a spectroscopic analysis system can be corrected using passive and/or active approaches. A passive approach can include determining and applying mathematical conversions to a recorded field spectrum. An active approach can include modifying one or more operating parameters of the spectroscopic analysis system to reduce frequency registration deviation.

公开(公告)号：US20230314221A1

公开(公告)日：2023-10-05

申请号：US18022793

申请日：2021-08-27

Applicant: THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK

Inventor： Wei LIN

IPC: G01J3/457 ,  A61B5/026 ,  A61B5/00

CPC classification number: G01J3/457 ,  A61B5/0261 ,  A61B5/0075

Abstract: DCS analyzer including a memory to store autocorrelation values, model parameters, fitting parameters, and simulated correlation values from a DCS model; a mean square error (MSE) module to compute MSE between theoretical autocorrelation values computed from the model parameters and measured autocorrelation values; a sorting module to sort three latest MSE values obtained from the MSE module and generate indexes of largest, medium, and smallest MSE values; a convergence checking module to determine whether convergence is reached in solving an autocorrelation equation; a search module to calculate αDB and β values at reflection, extension, contraction, and shrink locations; a comparison module to compare two latest MSE values and find new αDB and β values to replace values associated with a largest MSE; a state controller coupled with the memory and the modules to control an operation thereof; and an output buffer to present a fitted solution of the autocorrelation equation.

公开(公告)号：US20230160831A1

公开(公告)日：2023-05-25

申请号：US18095282

申请日：2023-01-10

Applicant: SAAM, Inc.

Inventor： John Coates

IPC: G01N21/85 ,  G01N21/25 ,  G01J3/00 ,  G01J3/02 ,  G01J3/42 ,  G01J3/457 ,  G01J3/36

CPC classification number: G01N21/8507 ,  G01N21/255 ,  G01J3/00 ,  G01J3/0291 ,  G01J3/42 ,  G01J3/457 ,  G01J3/0202 ,  G01J3/36 ,  G01J3/0205 ,  G01J3/0256 ,  G01N2201/08 ,  G01N21/359

Abstract: A remote sampling sensor for determining characteristics of a sample includes measurement optics and an insertion probe. The measurement optics are configured to emit light and detect returned light. The insertion probe includes a chamber, the chamber being configured to permit the sample to enter the chamber, an insertion tip at a distal end of the insertion probe, and a retro-reflective optic adjacent the insertion tip. The retro-reflective optic is configured to return the light from the measurement optics through the chamber to the measurement optics. The insertion probe is configured to be remotely located from the measurement optics.

公开(公告)号：US11653834B2

公开(公告)日：2023-05-23

申请号：US17462824

申请日：2021-08-31

Applicant: The Trustees of Columbia University in the City of New York

Inventor： Elizabeth Marjorie Clare Hillman ,  Sean A. Burgess

IPC: A61B5/00 ,  G01J3/28 ,  G01J3/42 ,  G01J3/457 ,  G02B26/10 ,  G02B23/24

CPC classification number: A61B5/0064 ,  A61B5/0066 ,  A61B5/0071 ,  A61B5/0073 ,  A61B5/0075 ,  A61B5/444 ,  A61B5/445 ,  G01J3/2889 ,  G01J3/42 ,  G01J3/457 ,  G02B23/2476 ,  G02B26/101 ,  A61B5/0088 ,  A61B2562/0238 ,  A61B2562/0242

Abstract: Optical imaging or spectroscopy described can use laminar optical tomography (LOT), diffuse correlation spectroscopy (DCS), or the like. An incident beam is scanned across a target. An orthogonal or oblique optical response can be obtained, such as concurrently at different distances from the incident beam. The optical response from multiple incident wavelengths can be concurrently obtained by dispersing the response wavelengths in a direction orthogonal to the response distances from the incident beam. Temporal correlation can be measured, from which flow and other parameters can be computed. An optical conduit can enable endoscopic or laparoscopic imaging or spectroscopy of internal target locations. An articulating arm can communicate the light for performing the LOT, DCS, or the like. The imaging can find use for skin cancer diagnosis, such as distinguishing lentigo maligna (LM) from lentigo maligna melanoma (LMM).

公开(公告)号：US20190052818A1

公开(公告)日：2019-02-14

申请号：US15672045

申请日：2017-08-08

Applicant: Sensors Unlimited, Inc.

Inventor： Curt Dvonch ,  Albert P. Allard

IPC: H04N5/272 ,  H04N7/18 ,  G06T11/60 ,  G01J3/457

CPC classification number: H04N5/272 ,  G01J3/457 ,  G06T11/60 ,  H04N5/2256 ,  H04N7/183

Abstract: An imaging method includes imaging a scene having a pulsed light source and associating a symbol with the light source. The image is enhanced by inserting a symbol into the image indicative of location of the pulsed light source in the scene. The symbol overlays the image in spatial registration with the location of the pulsed light source in the scene to augment indication of the location provided by the pulsed light source. Imaging systems are also described.