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公开(公告)号：KR101644094B1
公开(公告)日：2016-08-03
申请号：KR1020130114437
申请日：2013-09-26
Applicant: 경남과학기술대학교 산학협력단
Abstract: 본발명은육질이우수한버크셔종관련우수개체를조기에선별하기위한것으로, 본발명에의하면, 육질이우수한돼지의 SNP를선점할수 있고, 나아가상기 SNP를활용하여육질이우수한종돈을조기에선발하여육종할수 있으므로국내양돈산업의경쟁력을키울수 있음은물론값 싼수입돼지고기와도충분히경쟁할수 있다. 미래사회에서는유전자에대한소유권의선점이대단히중요한역할을수행하게되며, 특히어떠한형질과연관을나타내는유전자와이를활용한분석기술의가치는엄청나게높으므로, 이러한관점에서볼 때본 발명에서확인된유전자의변이는대단히중요한자료가될 수있고, 특히세계적으로확인되지않은신규한 SNP의선점은앞으로의돼지연구에높은가치가있다고할 수있다.
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22.发明公开
公开(公告)号：KR1020130110140A
公开(公告)日：2013-10-08
申请号：KR1020130114437
申请日：2013-09-26
Applicant: 경남과학기술대학교 산학협력단
CPC classification number: C12N15/11 , C12Q1/6888 , C12Q2600/156
Abstract: PURPOSE: An SNP marker related to pork meat quality and a method for selecting pigs with superior meat quality using the same are provided to acquire SNPs of a pig with superior meat quality and to early select and breed swine with high meat quality using the SNP, thereby developing competitiveness of domestic pork industry. CONSTITUTION: A primer for selecting pigs contains one base sequence among sequences of sequence numbers 38-74, containing 1st-18th bases. A method for selecting swine comprises the steps of: preparing gDNA from a pig sample; performing PCR of gDNA using the primer; scanning the PCR product by Illumina GoldenGat method using VeraCode Bead Plate; and confirming and classifying the genotypes of the scanned result using BeadStudio software (Illumina).
Abstract translation: 目的：提供与猪肉质量相关的SNP标记，以及使用该猪肉质量优良的猪选择猪的方法，以获取具有优良肉质的猪的SNP，并使用SNP早期选择和品种高品质的猪， 从而发展国内猪肉产业的竞争力。 构成：选择猪的引物在序列号38-74的序列中含有一个碱基序列，含有第1至18个碱基。 选择猪的方法包括以下步骤：从猪样品制备gDNA; 使用引物进行gDNA的PCR; 使用VeraCode Bead Plate通过Illumina GoldenGat方法扫描PCR产物; 并使用BeadStudio软件（Illumina）确认和分类扫描结果的基因型。
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公开(公告)号：KR1020100053341A
公开(公告)日：2010-05-20
申请号：KR1020080112406
申请日：2008-11-12
Applicant: 경남과학기술대학교 산학협력단
IPC: G01N33/70 , G01N33/561
Abstract: PURPOSE: A method for determining pork meat quality using a biomarker which is able to select pork of high quality is provided to reduce production cost by selecting and raising pork of high quality and to save time and cost for quality test. CONSTITUTION: A biomarker which is able to select pork having high quality is creatine and creatine kinase. A method for developing the biomarker comprises: a step of extracting total protein from pork loin; a step of dissolving and extracting protein powder and centrifuging; a step of collecting supernatant and performing electrophoresis; a step of dyeing with silver and comparing protein expression; a step of isolating protein spot from the two-dimensional gel and performing hydrolysis; a step of analyzing proteome profiling from pork muscle between high pH and low pH groups; a step of analyzing serum sample of the high pH and low pH groups with 1H-NMR; and a step of confirming that the metabolite compositions are different between the groups.
Abstract translation: 目的：提供一种能够选择高品质猪肉的生物标志物来确定猪肉质量的方法，通过选择和提高高品质猪肉来降低生产成本，节省质量测试的时间和成本。 构成：能够选择高品质猪肉的生物标志物是肌酸和肌酸激酶。 一种用于开发生物标志物的方法包括：从猪腰提取总蛋白质的步骤; 溶解和提取蛋白粉并离心分离的步骤; 收集上清液并进行电泳的步骤; 用银染色并比较蛋白质表达的步骤; 从二维凝胶中分离蛋白点并进行水解的步骤; 从高pH和低pH组之间分析猪肉蛋白质组图谱的步骤; 用1H-NMR分析高pH和低pH组的血清样品的步骤; 以及确认各组之间的代谢物组成不同的步骤。
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