公开(公告)号：KR1020100053341A

公开(公告)日：2010-05-20

申请号：KR1020080112406

申请日：2008-11-12

Applicant: 경남과학기술대학교 산학협력단

Inventor： 김철욱 ,  박화춘 ,  김진형 ,  최종순 ,  황금숙

IPC: G01N33/70 ,  G01N33/561

Abstract: PURPOSE: A method for determining pork meat quality using a biomarker which is able to select pork of high quality is provided to reduce production cost by selecting and raising pork of high quality and to save time and cost for quality test. CONSTITUTION: A biomarker which is able to select pork having high quality is creatine and creatine kinase. A method for developing the biomarker comprises: a step of extracting total protein from pork loin; a step of dissolving and extracting protein powder and centrifuging; a step of collecting supernatant and performing electrophoresis; a step of dyeing with silver and comparing protein expression; a step of isolating protein spot from the two-dimensional gel and performing hydrolysis; a step of analyzing proteome profiling from pork muscle between high pH and low pH groups; a step of analyzing serum sample of the high pH and low pH groups with 1H-NMR; and a step of confirming that the metabolite compositions are different between the groups.

Abstract translation: 目的：提供一种能够选择高品质猪肉的生物标志物来确定猪肉质量的方法，通过选择和提高高品质猪肉来降低生产成本，节省质量测试的时间和成本。 构成：能够选择高品质猪肉的生物标志物是肌酸和肌酸激酶。 一种用于开发生物标志物的方法包括：从猪腰提取总蛋白质的步骤; 溶解和提取蛋白粉并离心分离的步骤; 收集上清液并进行电泳的步骤; 用银染色并比较​​蛋白质表达的步骤; 从二维凝胶中分离蛋白点并进行水解的步骤; 从高pH和低pH组之间分析猪肉蛋白质组图谱的步骤; 用1H-NMR分析高pH和低pH组的血清样品的步骤; 以及确认各组之间的代谢物组成不同的步骤。

公开(公告)号：KR101091542B1

公开(公告)日：2011-12-14

申请号：KR1020080112406

申请日：2008-11-12

Applicant: 경남과학기술대학교 산학협력단

Inventor： 김철욱 ,  박화춘 ,  김진형 ,  최종순 ,  황금숙

IPC: G01N33/70 ,  G01N33/561

Abstract: 본 발명은 프로테오믹스와 메타볼로믹스 기술을 융합하여 시도된 육질 판별용 마커인 creatine(크레아틴)을 이용하여 돼지의 육질을 판정할 수 있는 우수한 육질을 가진 돼지를 선별할 수 있는 바이오 마커 및 상기 마커를 이용한 돼지의 육질판정방법에 관한 것으로 160여 개의 기존 등록된 대사체 프로파일을 high pH군과 low pH군의 혈청과 근육에서 비교 발굴하여 바이오 마커를 선별하였다.

公开(公告)号：KR1020110051977A

公开(公告)日：2011-05-18

申请号：KR1020090108819

申请日：2009-11-11

Applicant: 경남과학기술대학교 산학협력단

Inventor： 김철욱 ,  박화춘 ,  최종순 ,  황금숙 ,  박범영

IPC: G01N33/68

Abstract: PURPOSE: A method for analyzing Berkshire meat quality using serum free amino acid is provided to determine excellent Berkshire without analysis. CONSTITUTION: A method for Berksher meat quality using serum free amino acid comprises a step of analyzing pig meat quality using phenylalanine, valine, and leucine among free amino acid as a metabolic biomarker.

Abstract translation: 目的：提供一种使用无血清氨基酸分析伯克希尔肉质量的方法，以确定优秀的伯克郡，无需分析。 构成：使用无血清氨基酸的伯克谢肉质量的方法包括使用游离氨基酸中的苯丙氨酸，缬氨酸和亮氨酸作为代谢生物标志物来分析猪肉质量的步骤。