公开(公告)号：KR1020110051977A

公开(公告)日：2011-05-18

申请号：KR1020090108819

申请日：2009-11-11

Applicant: 경남과학기술대학교 산학협력단

Inventor： 김철욱 ,  박화춘 ,  최종순 ,  황금숙 ,  박범영

IPC: G01N33/68

Abstract: PURPOSE: A method for analyzing Berkshire meat quality using serum free amino acid is provided to determine excellent Berkshire without analysis. CONSTITUTION: A method for Berksher meat quality using serum free amino acid comprises a step of analyzing pig meat quality using phenylalanine, valine, and leucine among free amino acid as a metabolic biomarker.

Abstract translation: 目的：提供一种使用无血清氨基酸分析伯克希尔肉质量的方法，以确定优秀的伯克郡，无需分析。 构成：使用无血清氨基酸的伯克谢肉质量的方法包括使用游离氨基酸中的苯丙氨酸，缬氨酸和亮氨酸作为代谢生物标志物来分析猪肉质量的步骤。

公开(公告)号：KR1020110051978A

公开(公告)日：2011-05-18

申请号：KR1020090108820

申请日：2009-11-11

Applicant: 경남과학기술대학교 산학협력단

Inventor： 김철욱 ,  박화춘 ,  최종순 ,  박범영

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q1/6844 ,  C12Q2537/143 ,  C12Q2600/124

Abstract: PURPOSE: A gene marker for evaluating meat quality and Korean Berkshire genealogy and a diagnosis method are provided to be used as a marker for consisting Korean Berkshire. CONSTITUTION: A gene marker for evaluating meat quality is used for selecting an individual with excellent meat quality. A marker of Kit I among genes for evaluating pig meat quality is used as a common selection marker. A method for selecting excellent Berkshire comprises: a step of isolating DNA from pig blood or hair roots; a step of performing multiplex PCR using the marker having MQ KIT I and MQ KIT II; a step of analyzing genotype using a Genetic analyzer by One tube-One Injection; and a step of selecting excellent pig.

Abstract translation: 目的：提供用于评估肉质的基因标记和韩国伯克希尔家谱和诊断方法，用作韩国伯克郡的标志。 构成：用于评价肉质的基因标记用于选择具有优良肉质的个体。 用于评估猪肉质量的基因之间的Kit I的标记用作常见的选择标记。 选择优异的伯克希尔的方法包括：从猪血或发根分离DNA的步骤; 使用具有MQ KIT I和MQ KIT II的标记进行多重PCR的步骤; 使用One-One注射液使用Genetic分析仪分析基因型的步骤; 并选择优秀猪的一个步骤。

公开(公告)号：KR101644094B1

公开(公告)日：2016-08-03

申请号：KR1020130114437

申请日：2013-09-26

Applicant: 경남과학기술대학교 산학협력단

Inventor： 김철욱 ,  최종순 ,  박범영 ,  박화춘

IPC: C12N15/11 ,  C12Q1/68

Abstract: 본발명은육질이우수한버크셔종관련우수개체를조기에선별하기위한것으로, 본발명에의하면, 육질이우수한돼지의 SNP를선점할수 있고, 나아가상기 SNP를활용하여육질이우수한종돈을조기에선발하여육종할수 있으므로국내양돈산업의경쟁력을키울수 있음은물론값 싼수입돼지고기와도충분히경쟁할수 있다. 미래사회에서는유전자에대한소유권의선점이대단히중요한역할을수행하게되며, 특히어떠한형질과연관을나타내는유전자와이를활용한분석기술의가치는엄청나게높으므로, 이러한관점에서볼 때본 발명에서확인된유전자의변이는대단히중요한자료가될 수있고, 특히세계적으로확인되지않은신규한 SNP의선점은앞으로의돼지연구에높은가치가있다고할 수있다.

公开(公告)号：KR1020130110140A

公开(公告)日：2013-10-08

申请号：KR1020130114437

申请日：2013-09-26

Applicant: 경남과학기술대학교 산학협력단

Inventor： 김철욱 ,  최종순 ,  박범영 ,  박화춘

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12N15/11 ,  C12Q1/6888 ,  C12Q2600/156

Abstract: PURPOSE: An SNP marker related to pork meat quality and a method for selecting pigs with superior meat quality using the same are provided to acquire SNPs of a pig with superior meat quality and to early select and breed swine with high meat quality using the SNP, thereby developing competitiveness of domestic pork industry. CONSTITUTION: A primer for selecting pigs contains one base sequence among sequences of sequence numbers 38-74, containing 1st-18th bases. A method for selecting swine comprises the steps of: preparing gDNA from a pig sample; performing PCR of gDNA using the primer; scanning the PCR product by Illumina GoldenGat method using VeraCode Bead Plate; and confirming and classifying the genotypes of the scanned result using BeadStudio software (Illumina).

Abstract translation: 目的：提供与猪肉质量相关的SNP标记，以及使用该猪肉质量优良的猪选择猪的方法，以获取具有优良肉质的猪的SNP，并使用SNP早期选择和品种高品质的猪， 从而发展国内猪肉产业的竞争力。 构成：选择猪的引物在序列号38-74的序列中含有一个碱基序列，含有第1至18个碱基。 选择猪的方法包括以下步骤：从猪样品制备gDNA; 使用引物进行gDNA的PCR; 使用VeraCode Bead Plate通过Illumina GoldenGat方法扫描PCR产物; 并使用BeadStudio软件（Illumina）确认和分类扫描结果的基因型。