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    대하로부터 분리한 쿠니츠형 세린 프로테아제 저해인자
    24.
    发明授权
    대하로부터 분리한 쿠니츠형 세린 프로테아제 저해인자 有权
    昆虫型丝氨酸蛋白酶抑制剂从芬太尼

    公开(公告)号：KR101374194B1

    公开(公告)日：2014-03-13

    申请号：KR1020130055554

    申请日：2013-05-16

    Applicant: 대한민국(관리부서:국립수산과학원)

    Inventor： 공희정 ,  김영옥 ,  남보혜 ,  김우진 ,  김형수 ,  김봉석 ,  안철민

    IPC: C07K14/435 ,  C12N15/12 ,  C12N15/63 ,  A61K38/17

    Abstract: The present invention relates to a peptide representing serine protease inhibition activity and firstly separated from Fenneropenaeus chinensis, and proved anti-trypsin activity and anticoagulant activity. According to an embodiment of the present invention, a novel serine protease inhibitor is separated from Fenneropenaeus chinensis and identified, and the present invention proves effects of anti-trypsin activity and anticoagulant activity thereof, thereby efficiently applying the same as an active ingredient of anticoagulants. [Reference numerals] (AA) Anticoagulant activity(Prolongation of APTT(s))

    Abstract translation: 本发明涉及一种代表丝氨酸蛋白酶抑制活性的肽，首先从中华ensis ensis分离，证明了抗胰蛋白酶活性和抗凝血活性。 根据本发明的一个实施方案，将新的丝氨酸蛋白酶抑制剂从中华龙胆中分离出来，鉴定出来，本发明证明了抗胰蛋白酶活性和抗凝血活性的作用，从而有效地将其作为抗凝剂的活性成分施用。 （参考号）（AA）抗凝血活性（延长APTT）

    폴딩되는 큰 활성 단백질의 수용성 발현
    25.
    发明公开
    폴딩되는 큰 활성 단백질의 수용성 발현 有权
    大肠杆菌折叠活性蛋白的可溶性表达

    公开(公告)号：KR1020110124471A

    公开(公告)日：2011-11-17

    申请号：KR1020100043855

    申请日：2010-05-11

    Applicant: 대한민국(관리부서:국립수산과학원)

    Inventor： 이상준 ,  김영옥 ,  남보혜 ,  공희정 ,  김경길

    IPC: C12N15/63 ,  C12N15/11

    CPC classification number: C12N15/625 ,  C07K2319/02 ,  C07K2319/50 ,  C12N15/70 ,  C12P21/06

    Abstract: PURPOSE: A method for improving soluble expression of a target protein is provided to prevent insoluble precipitation of a recombinant protein and to improve recombinant protein secretion. CONSTITUTION: An expression vector for improving soluble expression and secretion of an active protein in which a membrane potential domain is folded contains: a promoter; and a gene construct which is operatively linked to the promoter and contains a polynucleotide encoding a leader polypeptide. A method for improving soluble expression and secretion of the active protein comprises: a step of designing a polynucleotide encoding a polypeptide with pI value of N-terminal to 2.00-6.00 and hydrophilic value to 1.00-2.00; a step of preparing a gene construct comprising the polynucleotide and a polynucleotide encoding the active protein; a step of operatively inserting the gene construct into an expression vector to prepare a recombinant expression vector; a step of transducing the recombinant expression vector to a host cell to form a transformant; and a step of culturing the transformant.

    Abstract translation: 目的：提供一种改善靶蛋白可溶性表达的方法，以防止重组蛋白的不溶性沉淀，并改善重组蛋白的分泌。 构成：用于改善其中折叠膜电位域的活性蛋白质的可溶性表达和分泌的表达载体包含：启动子; 和与启动子有效连接并含有编码前导多肽的多核苷酸的基因构建体。 一种用于改善活性蛋白质的可溶性表达和分泌的方法包括：将编码pI值为N-末端的多肽的多核苷酸设计为2.00-6.00，亲水性值设为1.00-2.00的步骤; 制备包含所述多核苷酸的基因构建体和编码所述活性蛋白质的多核苷酸的步骤; 将基因构建体可操作地插入表达载体以制备重组表达载体的步骤; 将重组表达载体转导到宿主细胞以形成转化体的步骤; 以及培养转化体的步骤。

    넙치 유래의 항균성 펩타이드인 베타디펜신을 암호화하는 신규한 유전자 및 그의 용도
    27.
    发明公开
    넙치 유래의 항균성 펩타이드인 베타디펜신을 암호화하는 신규한 유전자 및 그의 용도 有权
    新型基因编码橄榄球的β-缺陷及其用途

    公开(公告)号：KR1020110018646A

    公开(公告)日：2011-02-24

    申请号：KR1020090076221

    申请日：2009-08-18

    Applicant: 대한민국(관리부서:국립수산과학원)

    Inventor： 남보혜 ,  김영옥 ,  공희정 ,  김우진 ,  이상준 ,  김경길

    IPC: C07K14/46 ,  C12P21/04 ,  C12N15/12

    Abstract: PURPOSE: A novel gene encoding Paralichthys olivaceus-derived antibacterial peptide, bea-defensin is provided to ensure pivotal immune response function and to be used in developing natural antibiotics. CONSTITUTION: A Paralichthys olivaceus-derived antibacterial polypeptide comprises: a polypeptide encoded by cDNA having one of nucleic acid sequence among sequence numbers 1-5; a polypeptide encoded by gDNA having one of nucleic acid among sequence numbers 6-10; or a polypeptide having one of amino acid sequence among sequence numbers 11-15. The antibacterial polypeptide is anionic. Antibiotics or feed additives contain the antibacterial polypeptide, mutant thereof, or active fragment thereof as an active ingredient.

    Abstract translation: 目的：提供一种编码Paralichthys olivaceus来源的抗菌肽，bea-defensin的新基因，以确保关键的免疫应答功能，并用于开发天然抗生素。 构成：来自Paralichthys olivaceus的抗菌多肽包含：由序列号1-5之一的核酸序列之一的由cDNA编码的多肽; 由序列号6-10中具有核酸之一的gDNA编码的多肽; 或具有序列号11-15之一的氨基酸序列的多肽。 抗菌多肽是阴离子的。 抗生素或饲料添加剂含有作为活性成分的抗菌多肽，其突变体或其活性片段。

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