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公开(公告)号：KR1020130077207A
公开(公告)日：2013-07-09
申请号：KR1020110145794
申请日：2011-12-29
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6888 , C12Q2600/124 , C12Q2600/156
Abstract: PURPOSE: A SNP marker for polyacid entity selection of pig and an evaluation method using the same are provided to have high value on use at pre-selection of breeding pig by expressing high connection with a litter size, and to be predectied to have a big influence on the pig with SNP by generating protein variation on the exon region. CONSTITUTION: A pig polyacid entity selection method uses any selected one group a group comprised with SNP of sequence number of 1 through 54. A discriminating method of the pid polyacid entity is i) an extracting step of DNA from a subject; ii) an amplifying step of DNA including the base part of the sequcne number of 1 through 54 or the complemental strand of the base pair at the base mutation; iii) a determining step of the base mutation on the base sequence. The SNP set comprises the complex of any SNP comprised with the sequence number 1 through 54 for determining the pig polyacid entity. The primer set for the pig polyacid entity selection comprises an oligonucleotide including a base region of the sequence number 1 through 54 or the base region of the base mutation comprising the complementary strand with the base pair, or the base region with the sequence number 1 through 54 or the oligonucleotide having the base sequence of non-continuouse sequence of base 1 adjacent to the base mutation comoprising the complementary strand with the base pair.
Abstract translation: 目的：提供猪多聚体实体选择的SNP标记和使用其的评价方法，通过表达高度连带的仔猪大小，预先选择繁殖猪具有较高的价值，并被预测为具有较大的 通过在外显子区域产生蛋白质变异对SNP的猪产生影响。 构成：猪多酸实体选择方法使用任意选择的一个组，序列号为1至54的SNP组成的组。多肽实体的鉴别方法是i）来自受试者的DNA的提取步骤。 ii）在碱基突变时，包含1至54的序列的碱基部分或碱基对的互补链的DNA的扩增步骤; iii）碱基序列上的碱基突变的确定步骤。 SNP组包含用于确定猪多元酸实体的序列号1至54所包含的任何SNP的复合物。 猪多酸实体选择的引物组包括包含序列号1至54的碱基区域的碱基区域或包含与碱基对的互补链的碱基突变的碱基区域或序列号1至 54或与碱基突变相邻的碱基1的非连续序列的碱基序列与碱基对互补的寡核苷酸。
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公开(公告)号：KR1020130067998A
公开(公告)日：2013-06-25
申请号：KR1020110135104
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/2437 , A23K10/14 , C07K16/40 , C11D3/38645 , C12N15/67 , C12P1/00 , Y02E50/10
Abstract: PURPOSE: Cellulase which is a product of cel-KG02 gene is used as a feed additive and to industrially produce a detergent and biofuel. CONSTITUTION: A cellulase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes the cellulase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of cellulase from the host cell comprises a step of transducing the recombinant vector to the host cell and overexpresing cellulase.
Abstract translation: 目的：将作为cel-KG02基因产物的纤维素酶用作饲料添加剂，并在工业上生产洗涤剂和生物燃料。 构成：来自黑山羊的反刍动物胃微生物的纤维素酶蛋白具有序列号2的氨基酸序列。基因编码纤维素酶蛋白。 重组载体含有该基因。 宿主细胞被重组载体转化。 从宿主细胞产生大量纤维素酶的方法包括将重组载体转导到宿主细胞并过量表达纤维素酶的步骤。
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公开(公告)号：KR101229402B1
公开(公告)日：2013-02-05
申请号：KR1020100105005
申请日：2010-10-27
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 개의 유전질환의 하나인 고관절 이형성증을 조기에 진단할 수 있는 유전자 마커 개발에 관한 것으로서, 본 발명에 따른 분자표지를 이용할 경우 유전적으로 발생하는 진행성 질환인 고관절 이형성증의 위험성을 갖는 개에 대한 특별한 관리를 준비할 수 있고 번식을 위한 교배를 하는데 있어 중요한 유전적 정보를 예측하는데 기여할 수 있다.
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公开(公告)号：KR1020120131724A
公开(公告)日：2012-12-05
申请号：KR1020110050094
申请日：2011-05-26
Applicant: 대한민국(농촌진흥청장) , 박화춘
CPC classification number: C12Q1/6827 , C12Q1/6858 , C12Q2600/124 , C12Q2600/156
Abstract: PURPOSE: A gene marker and a method for diagnosing pork quality are provided to remarkably improve genetic ability of a breeding pig and to enhance pig selection accuracy. CONSTITUTION: A marker for diagnosing pork quality is a polynucleotide containing 8-500 continuous nucleotides containing SNP or a complementary polynucleotide thereof among polynucleotides selected sequence numbers 1-17. The SNP exists at 61th base of each sequence. A microarray for diagnosing pork quality contains the polynucleotide, a polypeptide encoded by the polynucleotide, or cDNA of the nucleotide. A method for determining pork quality comprises: a step of providing isolated nucleic acid samples; and a step of determining allele of each SNP of one or more polynucleotides selected from sequence numbers 1-17.
Abstract translation: 目的：提供一种基因标记和猪肉质量诊断方法，显着提高繁殖猪的遗传能力，提高猪选择精度。 构成：用于诊断猪肉质量的标记物是在选定的序列号1-17的多核苷酸中含有含有SNP的8-500个连续核苷酸或其互补多核苷酸的多核苷酸。 SNP存在于每个序列的第61个碱基。 用于诊断猪肉质量的微阵列包含多核苷酸，由多核苷酸编码的多肽或核苷酸的cDNA。 一种确定猪肉质量的方法包括：提供分离的核酸样品的步骤; 以及确定选自序列号1-17的一个或多个多核苷酸的每个SNP的等位基因的步骤。
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公开(公告)号：KR1020120072871A
公开(公告)日：2012-07-04
申请号：KR1020100134789
申请日：2010-12-24
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6876 , C12Q2600/124 , C12Q2600/156
Abstract: PURPOSE: A method for providing information for determining porcine unsaturated fatty acid content is provided to simply determine marbling score, oleic acid or linoleic acid content of pork and to early select and improve pigs of high quality. CONSTITUTION: A method for providing information for determining porcine unsaturated fatty acid content comprises: a step of isolating DNA from a pig; and a step of amplifying DNA using a prime pair for amplifying an amino acid sequence selected from sequence numbers 1-27 and 29-42 having an SNP selected among A↔C, A↔G, T↔C and T↔G in 61th base. The unsaturated fatty acid is oleic acid or linoleic acid. A method for providing information for determining pork of high quality comprises a step of isolating DNA from a pig and a step of amplifying DNA using the prime pair.
Abstract translation: 目的：提供确定猪不饱和脂肪酸含量信息的方法，简单地确定猪肉的大理石花纹分数，油酸或亚油酸含量，早期选择和提高猪的质量。 构成：提供用于测定猪不饱和脂肪酸含量的信息的方法包括：从猪中分离DNA的步骤; 以及使用素对扩增DNA的步骤，用于扩增在第61位碱基中选自A↔C，A↔G，T↔C和T↔G中的选自序列号1-27和29-42的氨基酸序列 。 不饱和脂肪酸是油酸或亚油酸。 提供用于确定高品质猪肉的信息的方法包括从猪分离DNA的步骤和使用素数对扩增DNA的步骤。
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Patent Agency Ranking
公开(公告)号：KR101149444B1
公开(公告)日：2012-06-27
申请号：KR1020090029553
申请日：2009-04-06
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A method for distinguishing bovine species by detecting mutation of fixed gene is provided to accurately identify bovine species. CONSTITUTION: A SNP(single nucleotide polymorphism) is used for distinguishing bovine species using information. A method for distinguishing bovine species comprises: a step of amplifying bovine DNA containing selected SNP through PCR-RFLP; a step of treating the nucleic acid with specific restriction enzyme.
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公开(公告)号：KR1020110139011A
公开(公告)日：2011-12-28
申请号：KR1020100059238
申请日：2010-06-22
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6827 , C12Q2600/156
Abstract: PURPOSE: A biomarker for diagnosing the content of pork marbling is provided to determine the content of pork marbling. CONSTITUTION: An SNP biomarker for determining the content of pork marbling contains one among sequences of sequence numbers 1-30. A method for selecting the SNP biomarker comprises: a step of determining SNPs relating to pork marbling content; a step of testing correlation with QTL(quantitative trait loci); and a step of analyzing the characteristic with QTL. A breeding pig is selected using the SNP biomarker. A kit for determining breeding pigs contains the SNP biomarker.
Abstract translation: 目的：提供用于诊断猪大理石花纹含量的生物标志物，以确定猪肉大理石花纹的含量。 构成：用于确定猪排大理石花纹的含量的SNP生物标志物包含序列号1-30的序列之一。 选择SNP生物标志物的方法包括：确定与猪排大花砾含量有关的SNP的步骤; 测定与QTL（数量性状位点）相关性的步骤; 并分析QTL特征的一个步骤。 使用SNP生物标志物选择繁殖猪。 用于确定繁殖猪的试剂盒含有SNP生物标志物。
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公开(公告)号：KR1020110139009A
公开(公告)日：2011-12-28
申请号：KR1020100059236
申请日：2010-06-22
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6827 , C12Q2600/156
Abstract: PURPOSE: A biomarker for diagnosing pork meat quality is provided to accurately measure genetic ability of a breeding pig and to determine the content of pork belly. CONSTITUTION: An SNP biomarker for determining the content of pork belly contains one or more of sequence numbers 1-10, containing quantitative trait loci(QTL) of pig chromosome No. 6. A method for selecting a biomarker comprises: a step of determining SNPs relating to pig meat quality; a step of detecting correlation with QTL; and a step of analyzing characteristic of pork belly content based on the result. A SNP biomarker is ALGA0103394, ALGA0118524, ALGA0123484, ASGA0029035, ASGA0029182, ASGA0029189, ASGA0029220, ASGA0095594, M1GA0008864, or MARC0043661.
Abstract translation: 目的：提供一种诊断猪肉质量的生物标志物，以准确测量养殖猪的遗传能力并确定猪肚的含量。 构成：用于测定猪肚含量的SNP生物标志物含有一个或多个序列号1-10，其含有猪染色体6的数量性状位点（QTL）。选择生物标志物的方法包括：测定SNP的步骤 涉及猪肉质量; 检测与QTL相关性的步骤; 并根据结果分析猪肚内容的特点。 SNP生物标志物是ALGA0103394，ALGA0118524，ALGA0123484，ASGA0029035，ASGA0029182，ASGA0029189，ASGA0029220，ASGA0095594，M1GA0008864或MARC0043661。
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公开(公告)号：KR101099595B1
公开(公告)日：2011-12-28
申请号：KR1020080129086
申请日：2008-12-18
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 돼지 근내 지방 함량에 관여하는 표지 인자에 관한 것으로서, 더욱 상세하게는 돼지 6번 염색체 내의 아데닐레이트 카이네이즈(Adenylate kinase) 유전자 전사 조절 영역의 전사개시 염기를 기준(+1)으로 -196 좌위를 포함하는 돼지 근내 지방 함량에 관여하는 표지 인자에 관한 것이다.
본 발명에 따른 분자표지를 이용할 경우 표현형평가에 의한 선발효과보다 25~35% 더 정확한 유전능력 측정이 가능하여 종돈의 유전능력 개량량을 획기적으로 늘릴 수 있으며, 등 지방 두께는 현재 육질을 평가하는 중요한 요인 중의 하나이므로 우수 종돈의 국내 육성에 크게 기여할 수 있다.
표지 인자, 근내 지방-
公开(公告)号：KR1020070062270A
公开(公告)日：2007-06-15
申请号：KR1020050122080
申请日：2005-12-12
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: A discrimination method of a cow species and a primer used thereto are provided to develop a DNA marker capable of discriminating the cow species, especially Hanwoo and non-Hanwoo, by analyzing change of genes fixed specifically to the cow species. The cow species is discriminated by digesting a SNP(single nucleotide polymorphism) selected from Y nucleotide of ADRB2 gene contained in SEQ ID NO:1, R nucleotide of CSN3 gene contained in SEQ ID NO:2, R nucleotide of MITF gene contained in SEQ ID NO:3, and R nucleotide of TFEB gene contained in SEQ ID NO:4 with restriction enzymes, PCR-amplifying the digested products using a primer which is designed to contain any one of the nucleotides in the SNP site, i.e. Y, R, R and R nucleotides as a 3'-terminal region, and analyzing the PCR products.
Abstract translation: 提供了一种鉴别牛种及其引物的鉴别方法，通过分析特定于牛种的基因的变化，开发出能够区分牛种，特别是Hanwoo和非Hanwoo的DNA标记。 通过消化选自SEQ ID NO：1中包含的ADRB2基因的Y核苷酸，SEQ ID NO：2中所含的CSN3基因的R核苷酸，SEQ ID NO：2中所含的MITF基因的R核苷酸，SEQ ID NO：1中含有的MITF基因的R核苷酸的SNP（单核苷酸多态性） SEQ ID NO：3所示的TFEB基因的R核苷酸和限制性内切酶的TFEB基因的R核苷酸，使用设计为含有SNP位点中任一个核苷酸的引物（即Y，R）进行PCR扩增消化产物 ，R和R核苷酸作为3'末端区域，并分析PCR产物。
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