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公开(公告)号：KR101918140B1
公开(公告)日：2018-11-16
申请号：KR1020180068300
申请日：2018-06-14
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C12Q1/689
Abstract: 본발명은퀴놀론계항생제내성균판별용 PNA 프로브및 이를이용한퀴놀론계항생제내성균판별방법에관한것으로, 더욱상세하게는비브리오균, 연쇄구균또는에드워드균의퀴놀론계항생제내성유전자마커를선정하여증폭시킨다음, 증폭산물을특이적으로인식하는 PNA를혼성화시키고, 혼성화된산물의온도조절을통해온도별융해곡선을얻고, 상기융해곡선의분석을통해융해온도로부터퀴놀론계항생제내성균을판별하는방법에관한것이다. 본발명에따르면비브리오균, 연쇄구균또는에드워드균의퀴놀론계항생제내성유무를간단·신속·정확하게판별할수 있으므로, 비브리오균, 연쇄구균또는에드워드균이유발하는수산생물전염병인비브리오병, 연쇄구균병또는에드워드병으로인해생기는피해를축소시키는데유용하다.
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23.发明授权연쇄구균 감염증 원인세균인 스트렙토코카스 파라우베리스의 판별 및 검출용 유전자 마커, 및 이를 이용한 원인세균의 판별 및 검출 방법 有权用于歧视和检测链球菌的基因标记及使用该方法鉴别和检测Parauberis的方法
公开(公告)号：KR101677951B1
公开(公告)日：2016-11-21
申请号：KR1020160046421
申请日：2016-04-15
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C12Q1/68
Abstract: 본발명은어류연쇄구균감염증원인세균의판별및 검출용유전자마커, 및이를이용한원인세균의판별및 검출방법에관한것으로, 더욱자세하게는어류연쇄구균감염증원인세균인스트렙토코카스파라우베리스()의 16S rDNA 유전자를코딩하는 DNA 염기서열중 세균특이적단일염기다형성(Single nucleotide polymorphism, SNP)을포함하는유전자마커를선정하여증폭시킨다음, 증폭산물을특이적으로인식하는펩티드핵산(Peptide Nucleic Acid, PNA)을혼성화시키고, 혼성화된산물의온도조절을통해온도별융해곡선을얻고, 상기얻은융해곡선의분석을통해융해온도로부터세균종을판별하거나어류의상기세균종의감염여부를검출하는방법에관한것이다. 본발명은어류질병원인세균인스트렙토코카스파라우베리스()의판별및/또는검출용유전자마커를선정하고, 상기유전자마커에특이적인펩티드핵산및 프라이머쌍을이용하여세균종마다서로다른형광의증폭및 융해곡선을나타내게함으로써어류질병원인세균을간단ㆍ신속ㆍ정확하게판별하고, 어류의상기세균의감염여부를검출할수 있는효과가있다.
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公开(公告)号：KR1020110136515A
公开(公告)日：2011-12-21
申请号：KR1020100056588
申请日：2010-06-15
Applicant: 대한민국(관리부서:국립수산과학원)
Abstract: PURPOSE: An inactivated vaccine for preventing vibrio harveyi infection, containing vibrio harveyi HN strain as an antigen is provided to reduce mortality of fishes and to enhance productivity of fishes. CONSTITUTION: A Vibrio harveyi HN(deposit number KCTC 11705BP) strain is isolated from flatfishes with vibrio harveyi infection. An inactivated vaccine for preventing vibrio herveyi infection contains the Vibrio harveyi HN(deposit number KCTC 11705BP) strain as an antigen. The vaccine further contains inactivated Streptococcus parauberis strain. A method for manufacturing the vaccine comprises a step of culturing the Vibrio harveyi HN(deposit number KCTC 11705BP) strain and inactivating. The medium for culturing the strain contains 50-200 umol of 2,2'-dipyridyl.
Abstract translation: 目的：提供一种用于预防弧菌弧菌感染的灭活疫苗，其包含哈氏弧菌HN菌株作为抗原，以减少鱼类的死亡率并提高鱼类的生产力。 构成：哈氏弧菌HN（保藏号KCTC 11705BP）菌株与哈氏弧菌感染的扁平鱼类分离。 用于预防弧菌感染感染的灭活疫苗含有作为抗原的Vibrio harveyi HN（保藏号KCTC 11705BP）菌株。 该疫苗还含有失活的欧洲白au链球菌菌株。 制备疫苗的方法包括培养哈氏弧菌HN（保藏号KCTC 11705BP）菌株并灭活的步骤。 用于培养菌株的培养基含有50-200μmol的2,2'-联吡啶。
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公开(公告)号：KR1020070088433A
公开(公告)日：2007-08-29
申请号：KR1020070080555
申请日：2007-08-10
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: A61K39/092 , A61K39/40 , Y10S424/827
Abstract: A method for preparing a mixture vaccine is provided to obtain the vaccine which is able to prevent Edwardsiellosis and beta-hemolysis streptococcal disease at the same time by one shot injection so that the health fish can be maintained. The method comprises the steps of: (a) after adding 20% glycerin to Edwardsiella tarda FP2018 and Streptococcus iniae JSL 0208, preserving it at a temperature of -85 deg.C; (b) after inoculating the preserved Edwardsiella tarda FP2018 and Streptococcus iniae JSL 0208 in a BHI(brain heart infusion) solid culture medium in which 1% of NaCl is added, culturing it at a temperature of 25 deg.C for 18-24 hours; (c) after respectively suspending cultured strains in sterile saline solution to prepare a suspended solution having the absorbance of 0.5, inoculating 5 ml of each of the obtained solution to 1 liter of a BHI liquid culture medium in which 1% of NaCl is added; (d) culturing each of the strains-inoculated each Erlenmeyer flask with mixing it in a shaking incubator at a temperature of 25 deg.C with the speed of 150 rotation per minute for 24 hours; (e) after respectively putting 37% formaldehyde in each of the strain culture solution to have the formaldehyde concentration of 0.4% and leaving it at room temperature for 1 hour, leaving it at 4 deg.C for 24 hours to inactivate each of the strains; (f) after centrifuging each of the inactivated strain culture solution with the speed of 6,000 rotation per minute for 20 minutes and measuring wet weight thereof, diluting it in sterile saline solution in which 0.02% of sodium azide is added to have the wet weight of 100mg/ml and storing it at a temperature of 4 deg.C; and (g) mixing a vaccine for Edwardsiellosis and a vaccine for beta-hemolysis Streptococcal disease in a ratio of 1:1.
Abstract translation: 提供一种制备混合疫苗的方法，以获得能够通过一次注射同时预防爱德华氏病和β-溶血性链球菌疾病的疫苗，从而保持健康的鱼类。 该方法包括以下步骤：（a）在Edwardsiella tarda FP2018和Streptococcus iniae JSL 0208中加入20％甘油后，在-85℃的温度下保存; （b）在保存的Edwardsiella tarda FP2018和Streptococcus iniae JSL 0208的BHI（脑心浸液）固体培养基中接种1％的NaCl后，在25℃的温度下培养18-24小时 ; （c）分别将培养菌株悬浮于无菌盐水溶液中以制备吸光度为0.5的悬浮液，将所得溶液5ml接种于1升加入1％NaCl的BHI液体培养基中; （d）将培养各菌株接种的每个锥形瓶，在振荡培养箱中在25℃的温度下以150转/分钟的速度混合24小时; （e）分别在每个菌株培养液中分别加入37％的甲醛使甲醛浓度为0.4％，并在室温下放置1小时，在4℃下放置24小时，使每个菌株失活 ; （f）以每分钟6,000转/分钟的速度离心每个灭活的菌株培养液20分钟并测量其湿重，将其稀释在无水盐水溶液中，其中加入0.02重量％的叠氮化钠以使湿重 100mg / ml，储存温度为4℃; 和（g）以1：1的比例混合用于爱德华氏病的疫苗和用于β-溶血性链球菌疾病的疫苗。
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Patent Agency Ranking
公开(公告)号：KR1020060065192A
公开(公告)日：2006-06-14
申请号：KR1020040103965
申请日：2004-12-10
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: C07K14/005 , A61K39/12 , A61K2039/523 , A61K2039/54 , A61K2039/552 , C12N2710/00022 , C12N2710/00034
Abstract: 본 발명은 한국형 돌돔 이리도바이러스의 면역원성을 갖는 단백질을 암호하는 유전자, 상기 유전자에 의해 암호되는 단백질, 상기 단백질에 의해 유래되는 합성 펩타이드, 상기 유전자를 포함하는 재조합 발현 벡터, 상기 재조합 벡터를 포함하는 형질전환체, 상기 형질전환체를 불활성화시켜 제조한 불활성화 백신 및 상기 백신을 이용하여 어류를 면역시키는 방법에 관한 것이다.
돌돔 이리도바이러스, 불활성화 백신-
公开(公告)号：KR102264146B1
公开(公告)日：2021-06-11
申请号：KR1020200169582
申请日：2020-12-07
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: G01N33/569 , G01N33/577 , C07K16/12 , C12N5/16 , C12R1/91
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公开(公告)号：KR101789736B1
公开(公告)日：2017-10-25
申请号：KR1020170110160
申请日：2017-08-30
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: A61K31/7048 , A23K20/163
Abstract: 본발명은이버멕틴을함유하는조피볼락의클라비네마마리에감염구제용조성물에관한것으로, 더상세하게는이버멕틴을유효성분으로함유하는조피볼락의클라비네마마리에감염구제용조성물및 조피볼락에이버멕틴을경구투여하여클라비네마마리에감염을구제하는방법에관한것이다. 본발명에따른조성물및 구제방법은조피볼락의클라비네마마리에감염의구제효과가매우우수하고, 조피볼락에부작용이없이안전하며, 경구투여가가능하여양식업계에서유용하게활용될수 있다.
Abstract translation: 本发明涉及一种用于感染的Clavinova向列谷物组合物补救含有伊维菌素石斑鱼，并且更特别地感染伊维菌素浮雕组合物和岩含有伊维菌素作为活性成分的向列石斑鱼晶粒的数码钢琴 口服本发明涉及一种通过口服缓解克氏综合征感染的方法。 组合物和根据本发明，消除感染的的Clavinova向列石斑鱼的马的效果非常优异，安全而对石斑鱼不利影响补救，可以在养殖业有用地用于允许口服给药。
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公开(公告)号：KR101737322B1
公开(公告)日：2017-05-18
申请号：KR1020160132026
申请日：2016-10-12
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C12Q1/70
Abstract: 본발명은잉어봄바이러스병바이러스검출용유전자마커, 및이를이용한바이러스의검출방법에관한것으로, 더욱자세하게는잉어봄바이러스병바이러스(Spring Viraemia of Carp Virus, SVCV)의특정핵산을증폭시킨다음, 증폭산물을특이적으로인식하는펩티드핵산(Peptide Nucleic Acid, PNA)을혼성화시키고, 혼성화된산물의온도조절을통해온도별융해곡선을얻고, 상기얻은융해곡선의분석을통해융해온도로부터잉어봄바이러스병바이러스를검출하거나잉어의상기바이러스의감염여부를확인하는방법에관한것이다. 본발명에의하면잉어봄바이러스병바이러스(SVCV)를간단·신속·정확하게검출함으로써, 잉어양식에가장큰 위협으로인식되고있는상기바이러스의감염및 상기바이러스에의해야기되는잉어봄바이러스병의확산을조기에차단할수 있는효과가있다.
Abstract translation: 本发明的鲤鱼春季病毒病病毒检测装置，用于遗传标记，以及涉及一种用于使用相同的检测病毒，更具体地，鲤鱼这样扩增的弹簧病毒病病毒（鲤鱼病毒的春季病毒血症，SVCV）的特定核酸，然后，放大的 肽核酸（肽核酸，PNA），其识别该产品用的杂交特异性和，从通过杂交产物的温度控制熔解曲线获得温度鲤鱼，并通过将得到的熔融曲线的熔融温度弹簧病毒疾病的分析 本发明涉及检测病毒或确认鲤鱼是否感染病毒的方法。 根据本发明的鲤鱼春季病毒病病毒（SVCV）的简单，快速，通过精确地检测，病毒，这是公认的锦鲤形式由病毒引起的早期鲤鱼春季病毒病的威胁和增殖的最佳感染 可以关闭。
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