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21.发明公开Ａ／ＳＷ／Ｋｏｒｅａ／ＳＣＪ01／09 및 Ａ／ｓｗｉｎｅ／Ｋｏｒｅａ／ＣＡＮ01／04의 유전자를 포함하는 재조합 돼지 신종인플루엔자 Ａ 바이러스 및 이를 포함하는 돼지 인플루엔자 바이러스 백신 有权重组猪流感病毒包含A / SW / KOREA / SCJ01 / 09和A / SWINE / KOREA / CAN01 / 04以及SWINE INFLUENZA包含该病毒的病毒疫苗的病毒的病毒
公开(公告)号：KR1020110131734A
公开(公告)日：2011-12-07
申请号：KR1020100051320
申请日：2010-05-31
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N7/01 , C12N15/44 , A61K39/145 , A61P31/16
Abstract: PURPOSE: A recombinant swine influenza A virus and a vaccine containing the same are provided to suppress proliferation of the virus and to prevent domestic pigs from the virus. CONSTITUTION: A recombinant swine influenza A virus contains gene of sequence numbers 1-6 among internal genes of A/swine/Korea/CAN01/04 (H1N1) of deposit number KCTC11165BP and virus surface protein gene of deposit number KCTC 11691BP. The virus surface protein gene contains HA of sequence number 7 and NA gene of sequence number 8. A vaccine for swine influenza virus contains the recombinant swine influenza A virus.
Abstract translation: 目的：提供重组型甲型流感病毒A和含有此类疫苗的疫苗，以抑制病毒的增殖并防止家蝇从病毒中获得。 构成：重组猪流感A病毒含有保藏号KCTC11165BP的A /猪/韩国/韩国/ CAN01 / 04（H1N1）的内部基因和保藏号KCTC 11691BP的病毒表面蛋白基因的序列号为1-6的基因。 病毒表面蛋白基因含有序列号7的HA和序列号8的NA基因。猪流感病毒疫苗含有重组猪甲型流感病毒。
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22.发明授权돼지전염성위장염바이러스, 유행성설사바이러스 및로타바이러스의 혼합생백신 및 이를 이용한 백신접종방법 有权돼지전염성위장염바이러스，유행성설사바이러스및로타바이러스의혼합생백신및이를이용한백신접종방돼
公开(公告)号：KR100737434B1
公开(公告)日：2007-07-09
申请号：KR1020060022530
申请日：2006-03-10
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K39/225 , A61K39/12
Abstract: A mixed live vaccine of transmissible gastroenteritis virus, porcine epidemic diarrhea virus and porcine rotavirus, and a vaccination method by using the same live vaccine are provided to prevent diarrhea of pigs at one time, reduce vaccination time, and minimize titer loss of attenuated viruses by using trehalose. The mixed live vaccine is prepared by mixing a transmissible gastroenteritis virus(KCTC 10889BP), a porcine epidemic diarrhea virus(KCTC 10891BP) and a porcine rotavirus(KCTC 10890BP) in a weight ratio of 1:1:1, and adding a trehalose phosphate glutamate gelatin mixture. The mixed live vaccine is vaccinated by vaccinating a mother pig with the mixed live vaccine one time, and vaccinating the first vaccinated mother pig with the vaccine of transmissible gastroenteritis virus(KCTC 10889BP) and porcine epidemic diarrhea virus(KCTC 10891BP) two times.
Abstract translation: 提供传染性胃肠炎病毒，猪流行性腹泻病毒和猪轮状病毒的混合活疫苗和使用相同活疫苗的疫苗接种方法，以一次预防猪腹泻，减少疫苗接种时间，并通过以下方式使减毒病毒的滴度损失最小化 使用海藻糖。 混合活疫苗通过混合重量比为1:1:1的传染性胃肠炎病毒（KCTC 10889BP），猪流行性腹泻病毒（KCTC 10891BP）和猪轮状病毒（KCTC 10890BP），并加入海藻糖磷酸酯 谷氨酸明胶混合物。 将混合活疫苗一次用混合活疫苗接种疫苗接种混合活疫苗，并用传染性胃肠炎病毒（KCTC 10889BP）和猪流行性腹泻病毒（KCTC 10891BP）疫苗接种第一次接种疫苗的母猪两次。
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公开(公告)号：KR1020050074846A
公开(公告)日：2005-07-19
申请号：KR1020040002757
申请日：2004-01-14
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K39/295
CPC classification number: A61K39/225 , A61K2039/521 , A61K2039/552 , Y10S424/815
Abstract: 본 발명은 불활화시킨 TGEV 및 PEDV를 항원으로 하는 혼합불활화 백신을 제공한다. 또한, 본 발명은 (1) TGEV를 ST세포에서 배양하고, PEDV를 Vero 세포에서 배양하는 단계; (2) 상기 단계 1의 배양액을 원심분리하여 고역가의 TGEV 및 PEDV (10
7.0 TCID
50 /ml 이상)를 함유한 상층액을 분리하는 단계; (3) 상기 단계 2에서 얻은 상층액에 바이러스 불활화제를 첨가하여 각 바이러스를 불활화시키는 단계; (4) 상기 불활화시킨 배양액을 ST 및 Vero 세포에 접종하여 세포변성유무를 확인하는 단계; 및 (5) 상기 불화화시킨 바이러스 배양액을 혼합하는 단계를 포함함을 특징으로 하는 혼합불활화 백신의 제조방법을 제공한다. 또한, 본 발명은 상기 본 발명에 따른 혼합불활화 백신을 돼지의 분만 5∼7주전 및 2∼3주전에 각각 2회 접종하여 모돈의 초유로부터 TGEV 및 PEDV 항체를 자돈에 전달하는 것을 특징으로 하는 돼지바이러스성 설사병의 예방방법을 제공한다.
상기 구성에 의하면, 주요 돼지 바이러스성 설사병의 원인체인 TGEV 및 PEDV를 충분히 예방하여 안전성이 우수하면서도 포유자돈에서의 설사병으로 인한 피해를 최소화하는 것이 가능해진다.-
公开(公告)号：KR102086089B1
公开(公告)日：2020-03-06
申请号：KR1020170059370
申请日：2017-05-12
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 메디안디노스틱
IPC: C12N5/16 , C07K16/10 , G01N33/569
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公开(公告)号：KR101814758B1
公开(公告)日：2018-01-05
申请号：KR1020140184709
申请日：2014-12-19
Applicant: 주식회사 메디안디노스틱 , 대한민국(농림축산식품부 농림축산검역본부장)
IPC: G01N33/569 , G01N33/533
Abstract: 본발명은돼지콜레라바이러스의 E2 단백질및 이에특이적인단일클론항체를이용하여돼지콜레라바이러스감염여부를진단하는방법및 이를구현하기위한진단키트에관한것이다. 본발명의측방유동검정방법(lateral flow assay)을이용한면역검사법은신속검사방법(rapid kit)으로서기존의효소면역분석법에비해신속한진단이가능하며비용이저렴한장점이있다. 따라서본 발명의진단방법및 진단키트는기존의돼지콜레라감염진단방법을대체할수 있을것이다.
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Patent Agency Ranking26.发明公开
公开(公告)号：KR1020110123918A
公开(公告)日：2011-11-16
申请号：KR1020100043406
申请日：2010-05-10
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N5/10 , C12N15/867 , A61K39/12 , A61P31/12
Abstract: PURPOSE: A PAM-pCD163 cell line for isolating porcine reproductive and respiratory syndrome(PRRS) virus is provided to develop a vaccine and to prevent PRRS. CONSTITUTION: A PAM-pCD163 cell line is prepared by expressing pCD163 in PAM cells through a retrovirus prepared by transforming plasmid containing pCD163 genes into HEK293 cells. The plasmid is pFB-Zeo-pCD163. A method for preparing the cell line comprises: a step of performing transfection of VSV-G glycoprotein and MMLVgag/pol gene, and a plasmid containing pCD163 into HEK293 cells to prepare retrovirus; and a step of inoculating the retrovirus into the PAM cells.
Abstract translation: 目的：提供用于分离猪繁殖与呼吸综合征（PRRS）病毒的PAM-pCD163细胞系，用于开发疫苗和预防PRRS。 构成：通过将含有pCD163基因的质粒转化到HEK293细胞中制备的逆转录病毒，通过在PAM细胞中表达pCD163来制备PAM-pCD163细胞系。 质粒是pFB-Zeo-pCD163。 一种制备细胞系的方法包括：将VSV-G糖蛋白和MMLVgag / pol基因转染的步骤和含有pCD163的质粒转染HEK293细胞以制备逆转录病毒; 以及将逆转录病毒接种到PAM细胞中的步骤。
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公开(公告)号：KR1020110091284A
公开(公告)日：2011-08-11
申请号：KR1020100011044
申请日：2010-02-05
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A scFv gene of monoclonal antibody 4G31 and a recombinant protein expressing the same are provided to neutralize RAV and to reduce and diagnose rabies virus. CONSTITUTION: A 4G31 scFv(single chain variable fragment antibody) gene which specifically neutralizes rabies virus has an amino acid sequence and a base of sequence number 16. The 4G31 scFv gene is a 4G31 monoclonal antibody containing a gene encoding a heavy chain variable region(VH) and a light chain variable region(VL). An expression vector expresses the 4G31 scFv gene. A recombinant protein is prepared by expressingthe 4G31 scFv gene in E.coli using the expression vector. A therapeutic agent for rabies virus contains the recombinant protein expressed from the 4G31 scFv gene.
Abstract translation: 目的：提供单克隆抗体4G31的scFv基因和表达其的重组蛋白，以中和RAV并减少和诊断狂犬病病毒。 构成：特异性中和狂犬病病毒的4G31 scFv（单链可变片段抗体）基因具有氨基酸序列和序列号16的碱基。4G31 scFv基因是含有编码重链可变区的基因的4G31单克隆抗体（ VH）和轻链可变区（VL）。 表达载体表达4G31 scFv基因。 通过使用表达载体在大肠杆菌中表达4G31scFv基因来制备重组蛋白。 狂犬病病毒的治疗剂含有从4G31 scFv基因表达的重组蛋白。
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公开(公告)号：KR1020080040942A
公开(公告)日：2008-05-09
申请号：KR1020060108942
申请日：2006-11-06
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 바이오노트
Abstract: A kit and a method for diagnosing a porcine epidemic diarrhea virus antigen are provided to diagnose the antigen conveniently and rapidly without a special testing equipment in a farm or a laboratory field. A kit for diagnosing a porcine epidemic diarrhea virus antigen is characterized in that a conjugate of a monoclonal antibody 6G15(deposition no. KCTC 10996BP) coupled to nucleocapside 58kDa of an epidemic diarrhea virus and a gold particle is attached to a reaction position of the porcine epidemic diarrhea virus antigen on a strip and a monoclonal antibody 9D11(deposition no. KCTC 10997BP) is attached to a position different from the coupling portion of the porcine epidemic diarrhea virus antigen and the monoclonal antibody 6G15. A method for diagnosing the porcine epidemic diarrhea virus antigen comprises the steps of: (a) loading a sample onto a detection pad on a strip; (b) reacting the conjugate of the monoclonal antibody 6G15 coupled to nucleocapside 58kDa of the epidemic diarrhea virus and the gold particle with the porcine epidemic diarrhea virus antigen in the sample; and (c) reacting the porcine epidemic diarrhea virus antigen, the monoclonal antibody 9D11 and the reaction product obtained from the step(b).
Abstract translation: 提供了一种用于诊断猪流行性腹泻病毒抗原的试剂盒和方法，用于在农场或实验室领域中没有特殊检测设备的情况下方便快速地诊断抗原。 一种用于诊断猪流行性腹泻病毒抗原的试剂盒的特征在于，将与流行性腹泻病毒和金颗粒的核衣壳58kDa偶联的单克隆抗体6G15（沉积编号KCTC 10996BP）的缀合物连接到猪的反应位置 将条带上的流行性腹泻病毒抗原和单克隆抗体9D11（沉积编号KCTC 10997BP）连接到与猪流行性腹泻病毒抗原和单克隆抗体6G15的偶联部分不同的位置。 用于诊断猪流行性腹泻病毒抗原的方法包括以下步骤：（a）将样品加载到条带上的检测垫上; （b）将与流行性腹泻病毒的金卡帕58kDa和金颗粒偶联的单克隆抗体6G15的缀合物与样品中的猪流行性腹泻病毒抗原反应; 和（c）使猪流行性腹泻病毒抗原，单克隆抗体9D11和从步骤（b）获得的反应产物反应。
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公开(公告)号：KR102061896B1
公开(公告)日：2020-01-09
申请号：KR1020180143325
申请日：2018-11-20
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12Q1/70 , C12Q1/6818
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