公开(公告)号：KR1020170022035A

公开(公告)日：2017-03-02

申请号：KR1020150116483

申请日：2015-08-19

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 양동군 ,  김하현 ,  조현례 ,  이승헌 ,  최성숙

IPC: C12Q1/70

Abstract: 본발명은게타바이러스검출용프라이머세트에관한것으로, 구체적으로서열번호 1의염기서열을갖는프라이머및 서열번호 2의염기서열을갖는프라이머를포함하고있어, RT-PCR을통해게타바이러스의 E1 유전자일부를특이적으로증폭할수 있고, 결과적으로다른유사바이러스들로부터게타바이러스만을정확하게검출할수 있도록하는프라이머세트에관한것이다. 본발명의프라이머세트, 조성물또는키트를사용하여 RT-PCR을수행하면시료내에게타바이러스가매우적은농도로존재하더라도매우민감도높게검출할수 있으며, 다른유사바이러스가존재하더라도게타바이러스만을정확하게검출해낼 수있다. 또한통상의 RT-PCR 방법을적용할수 있어쉽게빠르게검출할수 있다는장점이있다.

公开(公告)号：KR1020140017253A

公开(公告)日：2014-02-11

申请号：KR1020120083952

申请日：2012-07-31

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 양동군 ,  현방훈 ,  김하현 ,  조수동 ,  송재영

IPC: A61K39/235 ,  A61K39/39 ,  A61P31/00

CPC classification number: A61K39/235 ,  C12N15/66 ,  Y10S424/818

Abstract: The present invention relates to a recombinant adenovirus expressing a rabies virus gene and a vaccine composition for preventing or treating rabies comprising the same. The recombinant adenovirus according to the present invention, which is a recombinant virus of nucleotide sequences coding G protein and N protein of the rabies virus isolated from the country, effectively expresses G protein and N protein of the rabies virus and has a proliferation ability suitable for the use as a vaccine strain, and thus is suitable in mass production thereof. Further, the present invention has improved stability as compared with the exiting vaccine and thus can be used for oral administration, and has excellent ability to defend against the rabies virus at the time of vaccination.

Abstract translation: 本发明涉及表达狂犬病病毒基因的重组腺病毒和用于预防或治疗包含该狂犬病病毒基因的狂犬病的疫苗组合物。 根据本发明的重组腺病毒，其是从该国分离的狂犬病病毒的编码G蛋白和N蛋白的核苷酸序列的重组病毒，有效表达狂犬病病毒的G蛋白和N蛋白，并具有适合于 作为疫苗株的用途，因此适合于大量生产。 此外，与现有的疫苗相比，本发明具有改善的稳定性，因此可以用于口服给药，并且在接种疫苗时具有优异的防御狂犬病病毒的能力。

公开(公告)号：KR1020140004909A

公开(公告)日：2014-01-14

申请号：KR1020120072247

申请日：2012-07-03

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 양동군 ,  현방훈 ,  김하현 ,  조수동 ,  김인중 ,  송재영

IPC: C12N7/01 ,  A61K39/12 ,  G01N33/569

Abstract: The present invention relates to the recombinant rabies virus rERAG333Glu and a vaccine composition containing the same for preventing rabies. The recombinant rabies virus rERAG333Glu according to the present invention is a recombinant virus strain where the 333rd amino acid of G protein is replaced by glutamic acid. When manufacturing a vaccine from the virus, the vaccine has an excellent amount of virus so as to be suitable for mass-production, and has improved stability compared with the existing vaccines so as to be used for oral administration. Therefore, it can produce effects of solving problems of the existing rabies virus vaccines and being used as an effective vaccine for preventing rabies virus.

Abstract translation: 本发明涉及重组狂犬病病毒rERAG333Glu和含有该疫苗组合物的用于预防狂犬病的疫苗组合物。 根据本发明的重组狂犬病毒rERAG333Glu是重组病毒株，其中G蛋白的第333位氨基酸被谷氨酸代替。 当从病毒制造疫苗时，疫苗具有优良的病毒量以便适合批量生产，并且与现有疫苗相比具有改善的稳定性以便用于口服给药。 因此，它可以产生解决现有狂犬病毒疫苗问题的作用，并被用作预防狂犬病毒的有效疫苗。