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    곤충세포 발현 재조합 3ＡＢＣ 비구조단백질 항원 및 단클론항체를 이용한 구제역 진단방법
    2.
    发明授权
    곤충세포 발현 재조합 3ＡＢＣ 비구조단백질 항원 및 단클론항체를 이용한 구제역 진단방법 有权
    AB세포발현재조합3ABC비구조단백질항원및단클론항체를이용한구제역진단방곤충

    公开(公告)号：KR100430934B1

    公开(公告)日：2004-05-14

    申请号：KR1020010082976

    申请日：2001-12-21

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 권창희 ,  현방훈 ,  고영준 ,  이광녕 ,  구복경 ,  엄재구 ,  나진주 ,  남향미 ,  박지용 ,  계수정 ,  김인중 ,  이세영 ,  최광석 ,  손현주 ,  주이석

    IPC: C12N15/33

    Abstract: PURPOSE: Diagnostic methods of foot-and-mouth disease using recombinant 3ABC non-structural protein expressed in insect cells and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant baculovirus to be expressed in insect cells is prepared by co-transfection with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed by infection of the insect cells with the recombinant baculovirus. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the diluate on a plate; (2) reacting the testing serum with the plate; (3) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (4) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.

    Abstract translation: 目的：提供使用在昆虫细胞和单克隆抗体中表达的重组3ABC非结构蛋白的口蹄疫诊断方法，从而比现有方法更快速和准确地诊断口蹄疫。 构成：编码来源于韩国口蹄疫病毒O / SKR / 2000的重组3ABC非结构蛋白的基​​因具有SEQ ID NO：1的核苷酸序列。在昆虫细胞中表达的重组杆状病毒由 用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体共转染。通过用重组杆状病毒感染昆虫细胞表达重组3ABC非结构蛋白。 通过将在大肠杆菌中表达的重组3ABC非结构蛋白导入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合来制备杂交瘤细胞系3F-11（KCTC 10138BP）。 从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。 一种口蹄疫诊断方法，包括以下步骤：（1）将重组3ABC非结构蛋白稀释于包被缓冲液中，并将稀释液倒入平板中; （2）使测试血清与平板反应; （3）使与试验血清中的口蹄疫病毒抗体结合的缀合物与测试血清具有酶，放射性物质或荧光物质; 和（4）测量酶反应的强度，荧光反应或与缀合物的辐射反应。

    수포성 구내염 바이러스의 외피당단백질 항원 및 그에대한 모노클로날 항체를 이용하는 수포성 구내염의 진단방법
    3.
    发明公开
    수포성 구내염 바이러스의 외피당단백질 항원 및 그에대한 모노클로날 항체를 이용하는 수포성 구내염의 진단방법 有权
    使用乙酰胆碱酯酶抗生素血管病毒和单克隆抗体的乙型脑炎病毒的诊断方法

    公开(公告)号：KR1020040022247A

    公开(公告)日：2004-03-12

    申请号：KR1020010082714

    申请日：2001-12-21

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 권창희 ,  권병준 ,  고영준 ,  이세영 ,  김인중 ,  주이석

    IPC: A61K39/12

    Abstract: PURPOSE: A method of diagnosing vesicular stomatitis by performing an ELISA method using the recombinant envelope glycoprotein antigen of vesicular stomatitis virus(VSV) and monoclonal antibodies thereon is provided. Therefore, it permits quick, exact and specific antibody detection of the VSV and thus exact diagnosis of vesicular stomatitis through the detection. CONSTITUTION: The diagnosing method for vesicular stomatitis is achieved through a VSV detection method consisting of: attaching monoclonal antibodies against an envelope glycoprotein antigen of VSV on a solid supporter; binding the envelope glycoprotein antigen to the monoclonal antibodies attached on the solid supporter in the first step; reacting a sample containing an antibody against the envelope glycoprotein antigen with the envelope glycoprotein antigen of the second step; reacting a secondary antibody selected from the group consisting of a conjugate antibody attached with enzyme and a conjugate antibody attached with a luminescent material with the antibody of the third step.

    Abstract translation: 目的：提供使用水泡性口炎病毒（VSV）的重组包膜糖蛋白抗原及其单克隆抗体进行ELISA方法诊断水泡性口炎的方法。 因此，它可以快速，精确和特异性地检测VSV，从而通过检测确定水泡性口炎的诊断。 构成：水泡性口炎的诊断方法是通过VSV检测方法实现的，该方法包括：将固定支持物上VSV的包膜糖蛋白抗原的单克隆抗体连接起来; 在第一步中将包膜糖蛋白抗原与附着在固体支持物上的单克隆抗体结合; 使含有针对包膜糖蛋白抗原的抗体的样品与第二步的包膜糖蛋白抗原反应; 使选自由酶附着的结合抗体和附着有发光材料的缀合物抗体的第二抗体与第三步骤的抗体反应。

    재조합 광견병 바이러스 및 이를 포함하는 광견병 예방용 백신 조성물
    4.
    发明公开
    재조합 광견병 바이러스 및 이를 포함하는 광견병 예방용 백신 조성물 无效
    含有RABIES的重组病毒病毒和疫苗组合物

    公开(公告)号：KR1020140004909A

    公开(公告)日：2014-01-14

    申请号：KR1020120072247

    申请日：2012-07-03

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 양동군 ,  현방훈 ,  김하현 ,  조수동 ,  김인중 ,  송재영

    IPC: C12N7/01 ,  A61K39/12 ,  G01N33/569

    Abstract: The present invention relates to the recombinant rabies virus rERAG333Glu and a vaccine composition containing the same for preventing rabies. The recombinant rabies virus rERAG333Glu according to the present invention is a recombinant virus strain where the 333rd amino acid of G protein is replaced by glutamic acid. When manufacturing a vaccine from the virus, the vaccine has an excellent amount of virus so as to be suitable for mass-production, and has improved stability compared with the existing vaccines so as to be used for oral administration. Therefore, it can produce effects of solving problems of the existing rabies virus vaccines and being used as an effective vaccine for preventing rabies virus.

    Abstract translation: 本发明涉及重组狂犬病病毒rERAG333Glu和含有该疫苗组合物的用于预防狂犬病的疫苗组合物。 根据本发明的重组狂犬病毒rERAG333Glu是重组病毒株，其中G蛋白的第333位氨基酸被谷氨酸代替。 当从病毒制造疫苗时，疫苗具有优良的病毒量以便适合批量生产，并且与现有疫苗相比具有改善的稳定性以便用于口服给药。 因此，它可以产生解决现有狂犬病毒疫苗问题的作用，并被用作预防狂犬病毒的有效疫苗。

    아까바네, 츄잔, 아이노바이러스 균주 및 이를 이용한 혼합바이러스 백신의 제조방법
    7.
    发明公开
    아까바네, 츄잔, 아이노바이러스 균주 및 이를 이용한 혼합바이러스 백신의 제조방법 有权
    AKABANE VIRUS，CHUZAN VIRUS和AINO VIRUS的分离菌株，以及通过混合其制备病毒疫苗的方法

    公开(公告)号：KR1020080036353A

    公开(公告)日：2008-04-28

    申请号：KR1020060102854

    申请日：2006-10-23

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 김연희 ,  탁동섭 ,  임성인 ,  현방훈 ,  김인중 ,  송재영 ,  양동군 ,  권창희 ,  권준헌

    IPC: C12N7/01 ,  A61K39/12

    Abstract: A viral vaccine containing isolated virus strains is provided to improve use safety and immunogenicity by using inactivated viruses, and increase production yield of cows, so that the vaccine is useful for prevention of abortion or stillbirth in cows caused by viruses via mediation of Culicoides spp. A viral vaccine for prevention of abortion or stillbirth in cows contains cultured medium of inactivated virus strains of akabane virus(K-9)(KCTC 18117P), chuzan virus(KSA9910)(KCTC 18118P) and aino virus(YoungAM)(KCTC 18119P), and is produced by: inactivating akabane virus(K-9)(KCTC 18117P), chuzan virus(KSA9910)(KCTC 18118P) and aino virus(YoungAM)(KCTC 18119P) by adding 0.1M BEI(binary ethylene imine) prepared by solubilizing 2-BEA(2-bromoethylamine) in 0.2N NaOH, and treating the solution in a constant temperature water bath of 37 deg. C for 1 hour to them; mixing the 3 kinds of viruses in a weight ratio of 1:1:1; and adding 40-60 wt.% of IMS 1314 as an immunomodulator into the viral mixture.

    Abstract translation: 提供含有分离的病毒株的病毒疫苗，以通过使用灭活病毒提高使用安全性和免疫原性，并提高奶牛的产量，使得疫苗可用于通过Culicoides spp的调解来预防由病毒引起的奶牛的流产或死胎。 用于预防奶牛流产或死胎的病毒疫苗含有阿卡巴因病毒（K-9）（KCTC18117P），Chuzan病毒（KSA9910）（KCTC18118P）和Aino病毒（YoungCAM）（KCTC18119P）的灭活病毒株的培养基， 通过添加由0.1M BEI（二元乙烯亚胺）制备的阿卡巴因病毒（K-9）（KCTC 18117P），Chuzan病毒（KSA9910）（KCTC 18118P）和Aino病毒（YoungAM）（KCTC 18119P） 溶解在0.2N NaOH中的2-BEA（2-溴乙胺），并在37℃恒温水浴中处理该溶液。 C给他们1小时; 混合3种病毒，重量比为1：1：1; 并将40-60重量％的IMS 1314作为免疫调节剂加入到病毒混合物中。

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