-
公开(公告)号：KR1020010017747A
公开(公告)日：2001-03-05
申请号：KR1019990033412
申请日：1999-08-13
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: PURPOSE: Provided is an SCR(Sequence Characterized amplified Regions) primer for distinguishing Korean beef meat from milk cow meat, wherein band peculiar to Korean beef meat is appeared not in milk cow meat but in Korean beef meat. The SCR primer distinguishes them quickly and correctly. CONSTITUTION: A SCR primer is composed of the first primer having base sequence of CCA GAG CCT CGG GGA CTG AC in the direction from 5' toward 3' and the second primer having base sequence of CGC CCC CAG TAC ACC ATG AG in the direction from 5' toward 3'. The distinguishing method uses SCR primers, wherein the first primer is attached to 5' end of DNA of cow to be checked and the second primer is attached to 3' end of DNA of cow to be checked.
Abstract translation: 目的：提供一种用于区分韩国牛肉和牛奶肉的SCR（序列特征扩增区）引物，其中韩国牛肉特有的条纹不在牛奶肉中，而是在韩国牛肉中。 SCR引物快速正确地区分它们。 构成：SCR引物由具有CCA GAG CCT CGG GGA CTG AC的碱基序列的第一引物在5'至3'的方向组成，第二引物由CGC CCC CAG TAC ACC ATG AG的碱基序列沿着 5'朝3'。 区分方法使用SCR引物，其中第一引物连接到待检查的母牛的DNA的5'末端，第二引物连接到待检查的母牛的3'末端。
-
公开(公告)号：KR1019990070819A
公开(公告)日：1999-09-15
申请号：KR1019980005869
申请日：1998-02-25
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 소 육종을 조기에 간편하게 선발하도록 하는 소 육질 관련 DNA 표지인자와 그 표지인자를 이용한 비육우 조기선발 방법에 관한 것으로, 소 육질관련 DNA 표지인자는 NCBI Genbank에 등록번호 제 U503645호의 유전정보 3006호 부터 3365번 까지의 exon2에서 염기치환의 결과 생긴 유전자형(M형태)이다.
본 발명에 따라 미성숙 소들로 부터 요구되는 육질의 비육우를 육종하도록 조기에 선발할 수 있어서 비육우를 보다 경제적이고 효율적으로 육종할 수 있게 되는 효과가 있다.-
公开(公告)号：KR101341813B1
公开(公告)日：2013-12-17
申请号：KR1020110145779
申请日：2011-12-29
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은돼지의개체식별및 이력추적을위한단일염기다형유전자마커및 진단방법에관한것이다. 본발명에따른표지인자를활용하면표현형가에의한선발효과보다더 정확한유전능측정이가능하다. 또한돼지고기이력관리를위한유전자분석기법으로활용이가능하며, RFID 등의기능성개체식별도구와유전자분석기법의병행이가능하므로분석의신뢰도를높일수 있다.
-
公开(公告)号：KR101003364B1
公开(公告)日：2011-01-07
申请号：KR1020080038062
申请日：2008-04-24
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 한우 근육조직 부위별 특이 단백질 및 단백체의 특이적 조성을 구명하여 프로테옴 분석을 통해 근육의 부위를 판별할 수 있는 바이오 마커 단백질로서, 특히 한우 등심을 판별할 수 있는 바이오 마커 조성물에 관한 것이다.
본 발명에 의하면 한우 등심, 양지 및 홍두깨 근육조직에 대해 프로테옴 분석을 통해 부위판별이 가능하다. 더 나아가 본 발명에 의하면, 한우 등심, 양지 및 홍두깨 조직의 프로테옴 특성을 통한 축적조절기술 및 신약개발에 기초자료 제공할 수 있다.-
公开(公告)号：KR1020100133249A
公开(公告)日：2010-12-21
申请号：KR1020090052043
申请日：2009-06-11
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6827
Abstract: PURPOSE: A DNA marker for predicting and determining genetic variation which is a cause of albino(achromia,albinism) is provided to select and trace carrier containing genetic defect. CONSTITUTION: A molecular marker for selecting Hanwoo(Korean beef) albino is a polynucleotide having 20th base of DNA of sequence number 1 or complementary polynucleotide thereof. A kit for determining Hanwoo albino factor contains the polynucleotide of sequence number 1. A primer for determining Hanwoo albino factor has a base sequence of sequence number 2 or 3. The kit also contains the primer. A method for determining Hanwoo(Bos taurus coreanae) TYR gene mutation comprises: a step of extracting DNA from a subject; a step of amplifying DNA using a primer of sequence numbers 2 and 3; a step of treating the amplified gene with Mspl; and a step of confirming the product by electrophoresis.
Abstract translation: 目的：提供一种用于预测和确定遗传变异的DNA标记物，其是白化病（achromia，albinism）的原因，用于选择和追踪含有遗传缺陷的载体。 构成：用于选择Hanwoo（韩国牛）白蛋白的分子标记是具有序列号1的DNA的第20个碱基或其互补多核苷酸的多核苷酸。 用于确定Hanwoo白化因子的试剂盒含有序列号1的多核苷酸。用于测定Hanwoo白化因子的引物具有序列号2或3的碱基序列。试剂盒还含有引物。 确定Hanwoo（Bos taurus coreanae）TYR基因突变的方法包括：从受试者中提取DNA的步骤; 使用序列号2和3的引物扩增DNA的步骤; 用Mspl处理扩增的基因的步骤; 以及通过电泳确认产品的步骤。
-
Patent Agency Ranking
公开(公告)号：KR1020100080885A
公开(公告)日：2010-07-13
申请号：KR1020100056574
申请日：2010-06-15
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A biomarker composition for determining Hanwoo(Korean beef) eye round is provided to determine certain part of Hanwoo by proteomic analysis. CONSTITUTION: A biomarker composition for determining Hanwoo(Korean beef) comprises alpha-actinin-3, alpha-actinin-2, and calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase. Alpha-actinin-3 has an amino acid sequence of sequence number 6, 10297 kDa of molecular weight, and 5.31 of pI value. Alpha-actinin-2 has an amino acid sequence of sequence number 7, 103788kDa of molecular weight, and 5.31 of pI value.
Abstract translation: 目的：通过蛋白质组学分析来确定Hanwoo（韩国牛肉）眼睛的生物标志物组合，以确定Hanwoo的某些部分。 构成：用于测定Hanwoo（韩国牛肉）的生物标志物组合物包含α-辅肌动蛋白-3，α-辅肌动蛋白-2和钙/钙调蛋白依赖性3'，5'-环核苷酸磷酸二酯酶。 α-肌动蛋白-3具有序列号6,1097kDa的分子量的氨基酸序列和pI值的5.31。 α-辅肌动蛋白-2具有序列号7,103788kDa的分子量的氨基酸序列和pI值的5.31。
-
公开(公告)号：KR1020090112270A
公开(公告)日：2009-10-28
申请号：KR1020080038062
申请日：2008-04-24
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6888 , C12Q1/6827 , C12Q2600/124
Abstract: PURPOSE: A biomarker protein is provided to effectively determine muscle of Korean beef. CONSTITUTION: A biomarker protein for determining muscle of Korean beef contains arginase-2 mitochondrial precursor, entire amino acid sequence of angiopoietin-4 precursor, and cell activator CIDEB. The arginase-2 mitochondrial precursor has amino acid of the sequence number 1, 38592kDa of molecular weight, and 6.00 of pH concentration. The entire amino acid sequence of angiopoietin-4 precursor has an amino acid of the sequence number 2, 57,021kDa of molecular weight or 9.10 of pI value.
Abstract translation: 目的：提供生物标记蛋白，有效测定韩国牛肉的肌肉。 构成：用于测定韩国牛肉肌肉的生物标记蛋白含有精氨酸酶-2线粒体前体，血管生成素-4前体的全部氨基酸序列和细胞活化剂CIDEB。 精氨酸酶-2线粒体前体具有分子量为1，38592kDa，pH浓度为6.00的氨基酸。 血管生成素-4前体的全部氨基酸序列具有分子量为2，57,021kDa或pI值的9.10的氨基酸。
-
公开(公告)号：KR100874378B1
公开(公告)日：2008-12-18
申请号：KR1020070077942
申请日：2007-08-03
Applicant: 충북대학교 산학협력단 , 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6827 , C12Q2600/118 , C12Q2600/156
Abstract: A polynucleotide or its complementary polynucleotide, and a method for discriminating the Korean beef and the imported beef by using the polynucleotide are provided to allow the Korean beef and the imported beef to be discriminated easily with a high precision. A polynucleotide useful for the discrimination of the Korean beef and the imported beef comprises continuous 8-100 nucleotides which contains the 251th nucleotide of the first sequence, the second sequence, the fourth sequence, the sixth sequence, and the eighth sequence to the 13th sequence, the 256th nucleotide of the third sequence, the 287th nucleotide of the fifth sequence, or the 328th nucleotide of the seventh sequence.
Abstract translation: 提供多核苷酸或其互补多核苷酸，以及通过使用多核苷酸鉴别韩国牛肉和进口牛肉的方法，以便能够高精度地辨别韩国牛肉和进口牛肉。 用于区分韩国牛肉和进口牛肉的多核苷酸包括连续的8-100个核苷酸，其包含第一序列的第251个核苷酸，第二个序列，第四个序列，第六个序列和第十三个序列的第八个序列 ，第三序列的第256个核苷酸，第五个序列的第287个核苷酸或第七个序列的第328个核苷酸。
-
公开(公告)号：KR1020080006719A
公开(公告)日：2008-01-17
申请号：KR1020060065802
申请日：2006-07-13
Applicant: 대한민국(농촌진흥청장)
Abstract: A DNA marker is provided to determine an individual genetic trait of Korean native cattle by using genotypes of three independent loci of a FABP4(adipocyte-fatty acid binding protein) gene which has the significantly effect for carcass weight and marbling of the Korean native cattle, thereby being utilized for an early selection of a fatting beef and an accurate estimate of breeding values. A method for selecting a DNA marker of determining genetic traits comprises the steps of: (a) identifying a genotype of a linkage group detected from a Korean native cattle a fatty acid binding protein(FABP4) gene of SEQ ID : NO. 7 using the decoding of 17 SNP loci depicted at a Fig. 1; (b) after pre-denaturing the Korean native cattle FABP4 gene of SEQ ID : NO. 7 as a template using a PCR primer of SEQ ID : NOs. 1 to 6 at a temperature of 94 deg.C for 5 minutes, performing 35 times of a cycle where one minute annealing at a temperature of 58 deg.C and two-minute expanding at a temperature of 72 deg.C are done and then finally reacting it for 10 minutes at a temperature of 72 deg.C for PCR amplification; (c) after cleaving an amplification product obtained from the step(b) with an restriction enzyme such as Hpy188I, RsaI and NlaIII, subjecting it to an electrophoresis; and (d) obtaining a picture and an image of the electrophoresis product to determine an individual genotype through a restriction fragment length polymorphism analysis, a heteroduplex analysis, a single strand conformational polymorphism(SSCP) and a Taqman probe method. A DNA marker is selected by the method and constructed to recognize three loci of Hpy188I(C->G), RsaI(A->T) and NlaIII(A->G) described in the Fig.1 among variable regions of a FAB4 gene expressed in adipocyte.
Abstract translation: 提供DNA标记，通过使用FABP4（脂肪细胞 - 脂肪酸结合蛋白）基因的三个独立基因座的基因型来确定韩国本地牛的个体遗传特征，该基因对韩国本地牛的胴体重量和大理石花纹具有显着影响， 从而用于早期选择肥胖的牛肉和对育种价值的准确估计。 选择确定遗传性状的DNA标记的方法包括以下步骤：（a）鉴定从韩国本土牛检测的连锁群的基因型，其为SEQ ID NO：1的脂肪酸结合蛋白（FABP4）基因。 使用图7所示的17个SNP位点的解码。 1; （b）将SEQ ID NO：1的韩国本地牛FABP4基因预变性后， 7作为模板，使用SEQ ID：NO的PCR引物。 1〜6℃，温度94℃5分钟，进行35次循环，在58℃的温度下进行1分钟的退火，72℃的温度下进行2分钟的膨胀 最后在72℃的温度下反应10分钟进行PCR扩增; （c）用限制酶如Hpy188I，RsaI和NlaIII切割从步骤（b）获得的扩增产物后，进行电泳; 和（d）通过限制性片段长度多态性分析，异源双链分析，单链构象多态性（SSCP）和Taqman探针法获得电泳产物的图片和图像以确定个体基因型。 通过该方法选择DNA标记，并构建为在FAB4的可变区中识别图1中描述的Hpy188I（C-> G），RsaI（A-> A）和NlaIII（A-> G）的三个位点 在脂肪细胞中表达的基因。
-
公开(公告)号：KR1020070066353A
公开(公告)日：2007-06-27
申请号：KR1020050127393
申请日：2005-12-21
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: A method for identifying Korean native cattle and imported beef is provided to accurately identify the Korean native cattle derived from Bos taurus and the imported beef derived from Bos indicus by using structural differences of an RFLP genotype of a sex-determining region Y(SRY) gene. The method comprises the steps of: (a) collecting DNA from Korean native cattle and imported beef respectively; (b) respectively combining a forward primer having a nucleotide sequence of 5'-TTAGAACGCTTACACCGCATATTACTTC-3' and a backward primer having a nucleotide sequence of 5'-TAGTAAAATTGAGATAAAGAGCGCCTTTGTTAG-3' of an SRY gene with each of the collected DNA; (c) amplifying the combined product through PCR in which a nucleotide sequence portion of 1003-1803 in a Bos taurus DNA(registration number : AB039748) and a nucleotide sequence portion of 273-1073 of a Bos indicus DNA(registration number : AY079145) are amplified; and (d) after cutting the amplified product with an MseI restriction enzyme, analyzing polymorphism thereof to divide genotypes of the Korean native cattle and the imported beef.
Abstract translation: 提供了一种识别韩国本地牛和进口牛肉的方法，通过使用性别决定区Y（SRY）基因的RFLP基因型的结构差异来准确地识别来自Bos金牛的韩国本地牛和来自Bos indicus的进口牛肉 。 该方法包括以下步骤：（a）分别从韩国本地牛和进口牛收集DNA; （b）分别组合具有5'-TTAGAACGCTTACACCGCATATTACTTC-3'的核苷酸序列的正向引物和具有SRY基因的5'-TAGTAAAATTGAGATAAAGAGCGCCTTGTTAG-3'的核苷酸序列的反向引物与每个收集的DNA的组合; （c）通过PCR扩增组合产物，其中Bos Taurus DNA（登记号：AB039748）中的1003-1803的核苷酸序列部分和Bos indicus DNA的273-1073的核苷酸序列部分（登记号：AY079145） 被放大; 和（d）用MseI限制酶切割扩增产物后，分析其多态性以分割韩国本地牛和进口牛肉的基因型。
-
-
-
-
-
-
-
-
-
Search Results
31
records
(took 0.023 seconds)
