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公开(公告)号：KR100353475B1
公开(公告)日：2002-09-27
申请号：KR1020000003378
申请日：2000-01-25
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/63
Abstract: 본 발명은 형질전환체 선발이 용이하고 외래 유전자의 발현효율이 현저히 향상된 곤충 세포주용 형질전환 발현벡터 및 이 발현벡터를 함유하는 형질전환체에 관한 것이다.
본 발명에 의한 발현벡터 pAcIE1-Neo-hsp-Nuecin은 형질전환 세포주의 선발이 용이하고, 외래 유전자 산물의 발현효율이 높은 벡터로서, 이 발현벡터를 도입하여 형질전환시킨 곤충세포주는 계대 배양과 함께 영속적으로 항세균 단백질인 누에신을 생산할 수 있어, 계대 배양 및 대량 배양시 배지에 항생제를 첨가가 필요가 없는 장점이 있다. 또한, 발현벡터 pAcIE1-Neo-hsp는 발현효율이 높으므로, 외래 유전자 산물을 생산하기 위한 곤충세포주 형질전환용 벡터로서 유용하게 이용할 수 있다.
따라서, 본 발명에 의한 발현벡터와 형질전환 곤충 세포주는 연구용 뿐만 아니라 산업적으로 외래 유전자의 대량생산 발현계로 유용하게 이용될 수 있다.-
公开(公告)号：KR100261044B1
公开(公告)日：2000-07-01
申请号：KR1019970047219
申请日：1997-09-12
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/12
Abstract: PURPOSE: Provided is a cDNA of antimicrobial protein nuecin separated from Bombyx mori. And a recombinant antimicrobial protein nuecin is also provided which is effective on both gram positive and negative bacteria, so as to be applied to agricultural chemicals. CONSTITUTION: The cDNA of antimicrobial protein nuecin has the nucleotide sequence of figure (1) consisting of 852 bp and the amino acid sequence of figure (2) consisting of 214 amino acids. The expression vector pBacPAK8-nuecin shown in figure (3) contains the cDNA of antimicrobial protein nuecin. The recombinant virus vAc-nuecin is produced by transforming with the expression vector pBacPAK8-nuecin. The antimicrobial protein nuecin is produced by infecting Spodoptera frugiferda 9 with vAc-nuecin and collecting nuecin.
Abstract translation: 目的：提供从家蚕分离的抗微生物蛋白质素的cDNA。 并提供了一种对革兰氏阳性细菌和阴性菌有效的重组抗微生物蛋白质，以适用于农药。 构成：抗菌蛋白质核糖核酸的cDNA具有图（1）的核苷酸序列，由852bp和由214个氨基酸组成的图（2）的氨基酸序列组成。 图（3）所示的表达载体pBacPAK8-nuecin含有抗微生物蛋白质素的cDNA。 通过用表达载体pBacPAK8-nuecin转化产生重组病毒vAc-核糖核酸。 通过用vAc-nuecin感染草地贪夜蛾9并收集胡桃素来生产抗微生物蛋白质胡桃素。
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公开(公告)号：KR1020120124654A
公开(公告)日：2012-11-14
申请号：KR1020110042440
申请日：2011-05-04
Applicant: 대한민국(농촌진흥청장)
CPC classification number: G01N33/02 , C12Q1/6844 , C12Q2600/158 , G01N21/78 , G01N31/22
Abstract: PURPOSE: A method for analyzing mixed pollen is provided to analyze honey purity and to prevent production and distribution of false honey. CONSTITUTION: A method for analyzing mixed pollen for analyzing the purity and mixing state of honey comprises: a step of preparing a pollen analysis tool; a step of applying the mixed pollen to the pollen analysis tool and determining the characteristic of the mixed pollen; and a step of analyzing the purity and mixed state of honey. The mixed pollen is prepared by centrifuging at 12,000-14,000 rpm for 0.5-1 hours. [Reference numerals] (AA) Concentrate of honey-mixed pollen
Abstract translation: 目的：提供分析混合花粉的方法，分析蜂蜜的纯度，防止假蜂蜜的生产和分配。 构成：分析混合花粉分析蜂蜜的纯度和混合状态的方法，包括：制备花粉分析工具的步骤; 将混合花粉施用于花粉分析工具并确定混合花粉的特性的步骤; 并分析蜂蜜的纯度和混合状态的步骤。 混合花粉通过以12,000-14,000rpm离心0.5-1小时制备。 [参考号]（AA）蜂蜜混合花粉浓缩物
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公开(公告)号：KR1020110044085A
公开(公告)日：2011-04-28
申请号：KR1020090100905
申请日：2009-10-22
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A producing method of silk gland powder of matured silkworms is provided to easily obtain the silk gland powder by collecting silkworms right before a pupae stage. CONSTITUTION: A producing method of silk gland powder of matured silkworms comprises the following steps: breeding silkworms until the fifth instar, to obtain the matured silkworms; rapidly freezing the matured silkworms with liquid nitrogen, and storing; freeze-drying the matured silkworms; separating silk glands from the freeze-dried silkworms; and crushing the obtained silk glands.
Abstract translation: 目的：提供成熟蚕丝腺粉的生产方法，通过在蛹期前收集蚕丝，轻松获得丝腺粉末。 构成：成熟蚕丝腺粉的制备方法，包括以下步骤：将蚕培养至五龄，获得成熟蚕; 用液氮快速冷冻成熟的蚕，储存; 冷冻成熟的蚕; 从冷冻干蚕分离丝腺; 并粉碎获得的丝腺。
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公开(公告)号：KR1020100086841A
公开(公告)日：2010-08-02
申请号：KR1020090006276
申请日：2009-01-23
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A base of beehive for drone production is provided to enhance the productivity of manufactured goods using drones due to its suitable design for drone production. CONSTITUTION: A base of beehive for drone production includes: a beehive base frame(10); and a beehive base unit(20) for drones which is removably prepared inside the base frame and has a hexagonal groove for drones. The beehive base unit for drones includes: a hexagonal groove for drones; a pair of beehive base boards for drones(21) which is prepared to be piled up with each other; and an integration member which integrates a pair of the beehive base boards by bonding the boards in a releasable manner.
Abstract translation: 目的：提供无人机生产的蜂巢基地，以提高使用无人机的制成品的生产率，因为其适用于无人机的生产设计。 构成：无人机生产的蜂巢基地包括蜂巢基架（10）; 以及用于无人机的蜂巢基座单元（20），其可拆卸地制备在基架内部并具有用于无人机的六角形槽。 无人机的蜂巢基座包括：无人机的六角形槽; 一对准备相互堆积的无人机（21）的蜂巢基板; 以及集成部件，其通过以可释放的方式结合板来集成一对蜂巢基板。
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Patent Agency Ranking
公开(公告)号：KR1020100024536A
公开(公告)日：2010-03-08
申请号：KR1020080083145
申请日：2008-08-26
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/6827
Abstract: PURPOSE: A primer set for diagnosing cloudy wing virus(CWV) is provided to detect an RNA fragment of cloudy wing virus(CWV) and quickly and accurately diagnose a viral infection. CONSTITUTION: A primer set for diagnosing cloudy wing virus(CWV) infection contains an oligonucleotide primer set of sequence numbers 1 and 2. A kit for diagnosing CWV infection contains an oligonucleotide primer set, reverse transcriptase, and reagents for amplification. The reagent contains Tag DNA polymerase, dNTPs, and buffer. A method for diagnosing CWV infection comprises: a step of isolating entire RNA from a honeybee sample; a step of amplifying a target sequence through RT-PCR; and a step of detecting an amplified product.
Abstract translation: 目的：提供用于诊断多云翼病毒（CWV）的引物组，以检测多云翼病毒（CWV）的RNA片段，并快速准确地诊断病毒感染。 构成：用于诊断多云翼病毒（CWV）感染的引物组含有序列号1和2的寡核苷酸引物组。用于诊断CWV感染的试剂盒含有寡核苷酸引物组，逆转录酶和用于扩增的试剂。 试剂含有Tag DNA聚合酶，dNTP和缓冲液。 用于诊断CWV感染的方法包括：从蜜蜂样品中分离整个RNA的步骤; 通过RT-PCR扩增靶序列的步骤; 以及检测扩增产物的步骤。
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公开(公告)号：KR1020010074351A
公开(公告)日：2001-08-04
申请号：KR1020000003378
申请日：2000-01-25
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/63
CPC classification number: C12N15/866 , C12N2511/00
Abstract: PURPOSE: An expression vector for transforming an insect cell line is provided which has high expression efficiency and is thus used in mass-producing a foreign gene product. And an insect cell line transformant is also provided, which introduces the vector and produces antibacterial protein, Nuecin. CONSTITUTION: The expression vector pAcIE1-Neo-hsp consists of Baculovirus AcNPV IE1 promoter-neomycin resistant gene-Drosophila heat shock protein promoter-foreign gene inserting site(restriction enzyme EcoRV)-SV40 poly(A)-IE1 structural gene. The expression vector pAcIE1-Neo-hsp is constructed by: inserting neomycin resistant gene under the control of IE1 gene promoter of insect Baculovirus AcNPV; introducing a strong Drosophila heat shock protein promoter; placing a foreign gene inserting site, EcoRV site under the control of the promoter; and inserting animal virus, SV40 poly(A) site for the termination of transcription. The expression vector pAcIE1-Neo-hsp-Nuecin is constructed by inserting Nuecin cDNA gene into foreign gene inserting site, EcoRV site of pAcIE1-Neo-hsp.
Abstract translation: 目的：提供用于转化昆虫细胞系的表达载体，其具有高表达效率，因此用于批量生产外源基因产物。 还提供了一种昆虫细胞系转化体，其引入载体并产生抗菌蛋白质Nuecin。 构成：表达载体pAcIE1-Neo-hsp由杆状病毒AcNPV IE1启动子 - 新霉素抗性基因 - 果蝇热休克蛋白启动子 - 外源基因插入位点（限制酶EcoRV）-SV40聚（A）-IE1结构基因组成。 构建表达载体pAcIE1-Neo-hsp：将新霉素抗性基因插入昆虫杆状病毒AcNPV的IE1基因启动子控制下; 引入强果蝇热休克蛋白启动子; 将外源基因插入位点，EcoRV位点置于启动子的控制下; 并插入动物病毒，SV40 poly（A）位点终止转录。 通过将Nuecin cDNA基因插入外源基因插入位点，pAcIE1-Neo-hsp的EcoRV位点构建表达载体pAcIE1-Neo-hsp-Nuecin。
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28.发明授权누에 핵다각체병 바이러스 p10 유전자를 이용한 전이 벡터 및 제조방법 有权BOMBYX MORI NUCLEOCAPSIDE POLYHEDRON VIRUS VB2（KCTC8873P）的p10基因及其制备方法的过渡载体PBM10及其制备方法
公开(公告)号：KR100270928B1
公开(公告)日：2001-02-01
申请号：KR1019980019951
申请日：1998-05-30
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: Provided are transitional vector, pBm10 using p10 gene of Bombyx mori nucleocapside polyhedron virus VB2(KCTC8873P), which is useful for mass-producing materials useful in both biotechnology and insect biotechnology, and a method for preparation of the vector. CONSTITUTION: The transitional vector, pBm10, using p10 gene of Bombyx mori nucleocapside polyhedron virus VB2(KCTC8873P) is prepared by that: (a) 5' end of p10 gene of Bombyx mori nucleocapside polyhedron virus VB 2 is obtained by treating Pinpoint XalT-5'f vector with restriction enzyme HaeIII and BgIII, to produce a genetic fragment of 3Kb; treating this gene with restriction enzyme Kpn1; making blunt end of the gene by using T4 polymerase; and liking this to pBluescript SK+ which was cleaved by Bam HI and selecting recombinant plasmid (pSK-5'f) of 5.9 kb; and (b) 3' end is prepared by treating pGEM-3'f vector with restriction enzyme SmaI and SacI, to obtain a genetic fragment of 2.0kb and liking this to pSK-5'f vector which was cleaved by SmaI and SacI.
Abstract translation: 目的：提供使用Bombyx mori nucleocapside多面体病毒VB2（KCTC8873P）的p10基因的pBm10，其可用于生物技术和昆虫生物技术中有用的批量生产材料，以及用于制备载体的方法。 构成：通过以下方法制备使用Bombyx mori nucleocapside多面体病毒VB2（KCTC8873P）的p10基因的过表达载体pBm10：（a）通过处理Pinpoint XalT-片段获得家蚕蛹多核苷酸病毒VB2的p10基因的5'末端， 5'f载体与限制酶HaeIII和BgIII，以产生3Kb的遗传片段; 用限制酶Kpn1处理该基因; 通过使用T4聚合酶使基因平端化; 并喜欢这一点由Bam HI切割的pBluescript SK +，并选择5.9kb的重组质粒（pSK-5'f）; 通过用限制性内切酶SmaI和SacI处理pGEM-3'f载体，得到2.0kb的遗传片段，并将其转化成由SmaI和SacI切割的pSK-5'f载体，制备（b）3'末端。
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公开(公告)号：KR1019990086802A
公开(公告)日：1999-12-15
申请号：KR1019980019951
申请日：1998-05-30
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 국내산 누에 핵다각체병 바이러스 브이비2(KCTC8873P)의 p10 단백질 유전자를 클로닝하고 그 유전자 구조를 분석하였으며 이를 이용하여 외래유전자 발현용 전이벡터 피비엠l0을 제작하고, 그 전이벡터에 표지유전자로 베타-갈락토시다아제 유전자를 삽입하여 누에세포주에서 발현 여부를 분석한 결과, 그 전이벡터에의한 재조합바이러스는 다각체를 형성하면서 베타-갈락토시다아제를 발현하여 전이벡터로서 기능을 가지고 있음을 확인하였다. 따라서 본 발명은 국내산 누에 핵다각체병 바이러스 브이비2의 p10 단백질 유전자를 이용한 전이벡터 피비엠10과 이에의한 재조합바이러스 제작과 외래유전자 발현 및 유용물질 생산에 적용할 수 있다.
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公开(公告)号：KR1019990065232A
公开(公告)日：1999-08-05
申请号：KR1019980000444
申请日：1998-01-10
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/85
Abstract: 본 발명은 곤충 베큘로바이러스(AcNPV)의 다각체 단백질 유전자 프로모터의 조절하에 초록색 형광 단백질 유전자를 삽입한 재조합 바이러스(한국과학기술연구원 생명공학연구소 균주기탁번호 KCTC0418BP)를 제조하여, 이를 누에에 체강주사하면 재조합 바이러스의 소량 증식과 다각체 단백질 유전자 프로모터 조절에 의해 초록색 형광 단백질 유전자가 발현되는 유전자재조합 바이러스의 형광누에 및 제조방법 그리고 그 용도에 관한 발명이다. 형광누에는 누에품종에 따라서 그 발현정도가 다르며 특히 대성잠이 발현율과 전이에서 효과적이었고, 자외선 조사시 온몸에서 초록색 형광을 발하였다. 또 형광누에의 초록색 형광 단백질 유전자는 숙주곤충에 게놈에 삽입되는 기작으로 차세대에 전이됨을 밝혔다. 따라서 본 발명의 형광누에 개발 기술은 신기능 및 유전공학적으로 개량된 형질전환 곤충 창출에 활용될 수 있다.
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