-
公开(公告)号：KR102076764B1
公开(公告)日：2020-02-12
申请号：KR1020180068880
申请日：2018-06-15
Applicant: 서울대학교산학협력단
IPC: C07K14/705 , C12N15/81 , C12P7/56
-
-
Patent Agency Ranking23.发明公开
-
公开(公告)号：KR1020140026778A
公开(公告)日：2014-03-06
申请号：KR1020120092379
申请日：2012-08-23
Applicant: 서울대학교산학협력단
CPC classification number: Y02E50/17 , C12N15/81 , C12N9/0008 , C12P7/06 , C12Y102/01003
Abstract: The present invention relates to a yeast with a deleted ALD3 gene and a method of producing 2-phenylethanol using the same. Desirably, the present invention relates to the method of producing 2-phenylethanol using S.cerevisiae in which the ALD3 gene is deleted, and relates to a method for increasing a production of 2-phenylethanol by including phenylalanin in a culture medium as a single nitrogen source. In addition, a mutant with a deleted ALD2 is created in order to increase production efficiency, and the production amount of the 2-phenylethanol can be increased by overexpressing an ALD80 gene or additionally including a plasmid consisting of an ARO9 or ARO10 gene. The method of producing 2-phenylethanol according to the present invention has the advantage of being able to produce with a high yield at low costs under a more stable working condition than a chemical synthesis, while including fewer compounds harmful to a human body.
Abstract translation: 本发明涉及具有缺失的ALD3基因的酵母和使用其的2-苯基乙醇的制备方法。 理想地，本发明涉及使用缺失ALD3基因的酿酒酵母生产2-苯基乙醇的方法，涉及通过在培养基中包含苯丙氨酸作为单一氮气来提高2-苯基乙醇的生产方法 资源。 此外，为了提高生产效率，产生具有缺失的ALD2的突变体，并且可以通过过表达ALD80基因或另外包含由ARO9或ARO10基因组成的质粒来增加2-苯基乙醇的产生量。 根据本发明的2-苯基乙醇的制备方法具有能够在比化学合成更稳定的工作条件下以低成本生产高产率的优点，同时包含较少的对人体有害的化合物。
-
公开(公告)号：KR102112286B1
公开(公告)日：2020-05-19
申请号：KR1020180155169
申请日：2018-12-05
Applicant: 서울대학교산학협력단 , 경희대학교 산학협력단
-
公开(公告)号：KR1020160101608A
公开(公告)日：2016-08-25
申请号：KR1020150024535
申请日：2015-02-17
Applicant: 서울대학교산학협력단
IPC: C12N15/63 , C12N15/74 , C12N15/113
CPC classification number: C12N15/635 , C12N15/113 , C12N15/74
Abstract: Aro80 전사인자의결합부위에근거하여방향성아미노산유도성프로모터를제작하였다. 트립토판유도성프로모터의세기는 Aro80의결합부위수와플라스미드카피수(plasmid copy number), 트립토판의농도를조절함으로써넓은범위로조절될수 있다. 저플라스미드카피수(low plasmid copy number)와고 플라스미드카피수(high plasmid copy number)와다양한트립토판농도를사용하여, 29배까지의 EGFP(enhanced green fluorescent protein) 수용체의형광강도를합성 UC프로모터로부터얻을수 있는데, 그것은 4개의반복되는 Aro80 반결합부위(binding half site)와코어프로모터(core promoter)로구성된다. UC프로모터는사카로마이세스세레비지애에서아세토인(acetoin)을생산하기위해바실루스섭틸리스(Bacillus subtilis)로부터와유전자를발현하는데 활용되고, 그결과트립토판농도에의존적인아세토인역가(acetoin titers)가점진적으로증가된다. 트립토판유도성프로모터의발현정도의조절은대사공학에서유전자발현도의미세조정과경로의최적화를위한시기선택에유효하다.
Abstract translation: 本发明涉及由芳香族氨基酸诱导的启动子和使用该启动子的靶基因的转录方法，更具体地说，涉及一种用于在使用代谢工程中拓宽感应启动子选择的新型感应启动子。 启动子包含ARO80上游激活序列和ARO9核心启动子。
-
公开(公告)号：KR1020130012328A
公开(公告)日：2013-02-04
申请号：KR1020110073453
申请日：2011-07-25
Applicant: 서울대학교산학협력단
Abstract: PURPOSE: A composition containing a Yap1 mutant gene for enhancing HMF resistance of yeast is provided to enhance resistance to HMF which is a yeast growth inhibitor. CONSTITUTION: A composition for enhancing HMF(hydroxymethylfurfural) of yeast contains a Yap1 mutant gene encoding an amino acid sequence of sequence number 2 or an expression protein thereof. The Yap1 mutant gene has a base sequence of sequence number 1. The Yap1 mutant gene is inserted into an expression vector. A method for preparing HMF resistant yeast comprises a step of preparing a Yap1 mutant gene encoding an amino acid of sequence number 2; a step of preparing a recombinant vector containing the Yap1 mutant gene; and a step of transforming the recombinant vector into yeast.
Abstract translation: 目的：提供含有用于增强酵母HMF抗性的Yap1突变基因的组合物，以增强对作为酵母生长抑制剂的HMF的抗性。 构成：用于增强酵母HMF（羟甲基糠醛）的组合物含有编码序列号2的氨基酸序列的Yap1突变基因或其表达蛋白。 Yap1突变基因具有序列号1的碱基序列。将Yap1突变基因插入表达载体中。 制备HMF抗性酵母的方法包括制备编码序列号2的氨基酸的Yap1突变基因的步骤; 制备含有Yap1突变基因的重组载体的步骤; 以及将重组载体转化为酵母的步骤。
-
-
-
-
-
-
Search Results
27
records
(took 0.034 seconds)
