公开(公告)号：KR1020090090083A

公开(公告)日：2009-08-25

申请号：KR1020080015344

申请日：2008-02-20

Applicant: 한국과학기술연구원

Inventor： 류재천 ,  김연정 ,  전희경

IPC: C12N15/10 ,  C12N15/12 ,  C12Q1/68

CPC classification number: C12Q1/6837 ,  C12N15/1003 ,  C12Q1/6888 ,  C12Q2600/158

Abstract: A bio marker for identifying the exposure of naphthanlene is provided to monitor and determine the contamination of naphthalene through DNA microarray chip. A DNA microarray chip for identifying the exposure of naphthalene comprises oligonucleotide containing whole or part of biomarker gene sequence or its complementary molecule. A method for identifying the exposure of naphthalene comprises: a step of isolating RNA of somatic cell of test sample which is experimental group and somatic cell of control group; a step of synthesizing cDNA and probing with each different fluorescence; a step of hybridizing the cDNA and DNA microarray chip; a step of analyzing the DNA microarray chip; and a step of comparing the expression between control group and experimental group.

Abstract translation: 提供了用于鉴定萘醌暴露的生物标记物，用于通过DNA微阵列芯片监测和确定萘的污染。 用于鉴定萘暴露的DNA微阵列芯片包含含有全部或部分生物标记基因序列或其互补分子的寡核苷酸。 用于鉴定萘暴露的方法包括：分离作为实验组和对照组的体细胞的测试样品的体细胞的RNA的步骤; 合成cDNA并探索各种不同荧光的步骤; 将cDNA和DNA微阵列芯片杂交的步骤; 分析DNA微阵列芯片的步骤; 并比较对照组和实验组之间的表达。

公开(公告)号：KR1020090081234A

公开(公告)日：2009-07-28

申请号：KR1020080007205

申请日：2008-01-23

Applicant: 한국과학기술연구원

Inventor： 류재천 ,  김연정 ,  송미경 ,  샤르마사이렌드라나쓰

IPC: C12N15/12 ,  C12N15/10 ,  C12N15/09 ,  C12Q1/68

CPC classification number: C12N15/11 ,  C12Q1/6837 ,  C12Q1/6883 ,  C12Q2600/158

Abstract: A biomarker for identifying the exposure of ethylbenzene and identifying method are provided to determing the monitoring and harmfulness of ethylbenzene using the biomarker. A method for detecting the exposure of ethylbenzene using an biomarker comprises: a step of isolating RNAs from a test sample-derived human somatic cell and somatice cell of control group; a step of synthesizing cDNAs from the experimental group and control group and labeling with different fluorescence; a step of hybridizing with DNA microarray chip; and a step of analyzing DNA microarray chip.

Abstract translation: 提供了用于鉴定乙苯暴露的生物标志物和鉴定方法，以确定使用生物标志物的乙苯的监测和有害性。 使用生物标志物检测乙苯暴露的方法包括：从对照组的来自测试样品的人体细胞和体细胞中分离RNA的步骤; 从实验组和对照组合成cDNA并用不同荧光标记的步骤; 与DNA微阵列芯片杂交的步骤; 并分析DNA微阵列芯片的步骤。

公开(公告)号：KR1020090080679A

公开(公告)日：2009-07-27

申请号：KR1020080006573

申请日：2008-01-22

Applicant: 한국과학기술연구원

Inventor： 류재천 ,  김연정 ,  송미경 ,  샤르마사이렌드라나쓰

IPC: C12N15/12 ,  C12N15/09 ,  C12Q1/68

CPC classification number: C12Q1/6837 ,  C12N15/09 ,  C12Q1/6888 ,  C12Q2600/158

Abstract: A biomarker for identifying the exposure of trichloroethylene is provided to detect trichloroethylene using genes of which expressions are overexpressed or underexpression by the trichloroethylene. A DNA microarray chip for identifying the exposure to a trichloroethylene is aggregate of a molecule containing oligonulcleotide having entire or a portion of biomarker gene sequence for identifying the exposure of trichloroethylene or its trichloroethylene. A method for identifying the exposure of trichloroethylene comprises: a step of isolating RNAs from human somatic cell isolated from a test sample (experimental group) and somatic cell of control group; a step of synthesizing RNAs to cDNAs and labeling the cDNAs of experimental group and control group; a step of hybridizing the cDNAs on DNA microarray chip; a step of analyzing DNA microarray chip; and a step of comparing the expression. The human somatic cell is derived from bone marrow or cell or tissue of leukemia patient.

Abstract translation: 提供用于鉴定三氯乙烯暴露的生物标志物，以检测三氯乙烯，其中使用表达被三氯乙烯过表达或低表达的基因。 用于鉴定暴露于三氯乙烯的DNA微阵列芯片是含有寡核苷酸的分子的聚集体，其具有用于鉴定三氯乙烯或其三氯乙烯暴露的全部或部分生物标志物基因序列。 用于鉴定三氯乙烯暴露的方法包括：从测试样品（实验组）和对照组体细胞分离的人体细胞中分离RNA的步骤; 将cDNA合成cDNA的步骤，并对实验组和对照组的cDNA进行标记; 在DNA微阵列芯片上杂交cDNAs的步骤; 分析DNA微阵列芯片的步骤; 以及比较表达的步骤。 人体细胞源自白血病患者的骨髓或细胞或组织。

公开(公告)号：KR1020090071808A

公开(公告)日：2009-07-02

申请号：KR1020070139711

申请日：2007-12-28

Applicant: 한국과학기술연구원

Inventor： 류재천 ,  김연정 ,  송미

IPC: C12N15/12 ,  C12N15/11 ,  C12Q1/68

CPC classification number: C40B40/06 ,  C12Q1/6837 ,  C12Q2561/113 ,  C12Q2563/107

Abstract: A marker gene for screening a drug which induces pulmonary toxicity is provided to monitor and determine a drug or chemical substance causing pulmonary toxicity by increasing or decreasing the expression of the marker by nitofurantoin. An expression of marker gene for screening a drug which causes pulmonary toxicity is increased or decreased by the drug treatment. The drug causing pulmonary toxicity is nitrofurantoin. A DNA microarray chip for screening the drug comprises oligonucleotide containing whole or a part of marker gene sequence or their complement strand. A method for screening the drug comprises: a step of treating a subject compound in a human bronchiolar cell or lung cell; a step of isolating RNA from the subject compound-treated experimental group and untreated-control group; a step of synthesizing cDNA and labeling with each different fluorescence; a step of hybridizing cDNA with DNA microarray; a step of analyzing DNA microarray; and a step of comparing the expressions between the experimental group and control group.

Abstract translation: 提供用于筛选诱导肺毒性的药物的标记基因，通过增加或减少由硝呋喃素标记物的表达来监测和测定引起肺部毒性的药物或化学物质。 用于筛选引起肺毒性的药物的标记基因的表达通过药物治疗而增加或减少。 导致肺部毒性的药物是呋喃妥因。 用于筛选药物的DNA微阵列芯片包含含有全部或部分标记基因序列或其互补链的寡核苷酸。 筛选药物的方法包括：处理人支气管细胞或肺细胞中的受试化合物的步骤; 从受试化合物处理的实验组和未处理对照组中分离RNA的步骤; 合成cDNA并用各种不同荧光标记的步骤; 将cDNA与DNA微阵列杂交的步骤; 分析DNA微阵列的一个步骤; 并比较实验组与对照组之间的表达。

公开(公告)号：KR1020080112764A

公开(公告)日：2008-12-26

申请号：KR1020070061573

申请日：2007-06-22

Applicant: 한국과학기술연구원

Inventor： 류재천 ,  김연정 ,  송미 ,  이하은

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  G01N33/5008

Abstract: Marker genes for screening drug inducing cardiotoxicity and a screening method thereof are provided to monitor and determine drugs or chemical substances having the risk of heart toxicity by using the reaction genes selected through a DNA microarray chip. A screening method drug inducing cardiotoxicity comprises (1) a step for processing an object compound in HUVEC; (2) a step for separating RNA from the experimental group cell which processes the object compound of the step (1) and the control cell which does not process the object compound; (3) a step for respectively making the experimental group and control group by different fluorescent materials while synthesizing RNA of the experimental group of the step (2) and the control group to cDNA; (4) a step for mixing the cDNA respectively marked by different fluorescent materials of the step (3) with the DNA microarray; (5) a step for analyzing the DNA reacted microarray of the step (4); and (6) a step for comparing and confirming the expressed grade of the marker gene with the control group.

Abstract translation: 提供用于筛选药物诱导心脏毒性的标记基因及其筛选方法，以通过使用通过DNA微阵列芯片选择的反应基因来监测和测定具有心脏毒性风险的药物或化学物质。 筛选方法药物诱导心脏毒性包括（1）在HUVEC中加工目标化合物的步骤; （2）分离来自处理步骤（1）的目标化合物和不处理目标化合物的对照细胞的实验组细胞的RNA的步骤; （3）步骤（2）和对照组合成RNA，分别使不同荧光物质分别制作实验组和对照组; （4）将由步骤（3）的不同荧光材料分别标记的cDNA与DNA微阵列混合的步骤; （5）分析步骤（4）的DNA反应的微阵列的步骤; 和（6）比较和确认标记基因与对照组的表达等级的步骤。

公开(公告)号：KR1020080045988A

公开(公告)日：2008-05-26

申请号：KR1020060115346

申请日：2006-11-21

Applicant: 한국과학기술연구원

Inventor： 류재천 ,  김연정

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12Q2561/113

Abstract: A biomarker is provided to be useful for monitoring methotrexate and determining harmfulness of the methotrexate, thereby being used as a tool for identifying toxicity action mechanism caused by the methotrexate. A biomarker for screening methotrexate includes a gene selected from the group consisting of Genebank NM_004759, Genebank AK122828, Genebank NM_003311, Genebank NM_003506, Genebank NM_005438, Genebank NM_030821, Genebank NM_016205, Genebank AK027210, Genebank NM_018367, Genebank M92424, Genebank NM_000826, Genebank BC036030, Genebank NM_004167, Genebank AB057597, Genebank D26070, Genebank NM_207111, Genebank NM_002511, Genebank NM_000575, Genebank NM_138961, Genebank NM_001024736, Genebank BC002726, Genebank NM_033131, Genebank BC030595, Genebank NM_000710, Genebank AK096323, Genebank NM_078467, Genebank NM_000425, Genebank NM_000899, Genebank NM_002562, Genebank CR594190, Genebank AL832585, Genebank NM_198859, Genebank NM_018667, Genebank NM_001295, Genebank NM_001911, Genebank NM_001759, Genebank NM_000641, Genebank S78234, Genebank AB209361, Genebank BX649005, Genebank AK127679, Genebank AK126462, Genebank NM_003013, Genebank NM_006162, Genebank BC008678, Genebank AK022486, Genebank NM_001315, Genebank BC026283, Genebank AK092791, Genebank BX648822, Genebank BC036078, Genebank L25664, Genebank NM_005419, Genebank CR598645, Genebank NM_031468, Genebank AL833391, Genebank BC033058, Genebank AK127004, Genebank NM_004064, Genebank NM_004360, Genebank BC031405, Genebank BC075814, Genebank M38056, Genebank AB209599, Genebank AY358836, Genebank NM_002758, Genebank NM_006614, Genebank NM_080425, Genebank NM_004526 Genebank L19315, Genebank NM_006120, Genebank NM_002760, Genebank AF041245, Genebank NM_000889, and Genebank AL136787. A total or a portion of the biomarker gene sequence or a complimentary strand molecule thereof is integrated into a DNA microarray chip. A method for screening volatile organic compounds comprises the steps of: (a) treating human bronchial epithelial cells with a compound to be tested; (b) isolating RNA respectively from the test group cells treated with the compound to be tested and control group cells; (c) labelling the test group and the control group, respectively, with a fluorescent material different from each other with synthesizing the RNA of the test group and the control group into cDNA; (d) hybridizing each the cDNA(s) labelled with the fluorescent material into the DNA microarray chip; (e) analyzing the reacted DNA microarray chip; and (f) comparing the expression degree of the biomarker from the data analyzed by the step(e) with that of the control group.

Abstract translation: 提供了一种生物标志物，可用于监测甲氨蝶呤和确定甲氨蝶呤的有害性，从而被用作识别甲氨蝶呤引起的毒性作用机制的工具。 用于筛选甲氨蝶呤的生物标志物包括选自Genebank NM_004759，Genebank AK122828，Genebank NM_003311，Genebank NM_003506，Genebank NM_005438，Genebank NM_030821，Genebank NM_016205，Genebank AK027210，Genebank NM_018367，Genebank M92424，Genebank NM_000826，Genebank BC036030， Genebank NM_004167，Genebank NM_004167，Genebank AB057597，Genebank D26070，Genebank NM_207111，Genebank NM_002511，Genebank NM_000575，Genebank NM_138961，Genebank NM_001024736，Genebank BC002726，Genebank NM_033131，Genebank BC030595，Genebank NM_000710，Genebank AK096323，Genebank NM_078467，Genebank NM_000425，Genebank NM_000899，Genebank NM_002562 ，Genebank CR594190，Genebank AL832585，Genebank NM_198859，Genebank NM_018667，Genebank NM_001295，Genebank NM_001911，Genebank NM_001759，Genebank NM_000641，Genebank S78234，Genebank AB209361，Genebank BX649005，Genebank AK127679，Genebank AK126462，Genebank NM_003013，Genebank NM_006162，Genebank BC008678，Genebank AK022486，Genebank NM-001315，Genebank BC026283，Genebank BC026283，Genebank BC026283，Genebank BC026283，Genebank BC026283，Genebank BC026283，Genebank BC026283，Genebank BC026283，Genebank， Genebank AB209599，Genebank AY358836，Genebank NM_002758，Genebank NM_006614，Genebank NM_080425，Genebank NM_004526 Genebank L19315，Genebank NM_006120，Genebank NM_002760，Genebank AF041245，Genebank NM_000889和Genebank AL136787。 生物标记基因序列的全部或部分或其互补链分子整合到DNA微阵列芯片中。 用于筛选挥发性有机化合物的方法包括以下步骤：（a）用待测化合物处理人支气管上皮细胞; （b）分别从用待测化合物和对照组细胞处理的试验组细胞中分离RNA; （c）分别用不同的荧光材料标记试验组和对照组，将试验组和对照组的RNA合成cDNA; （d）将用荧光物质标记的每个cDNA与DNA微阵列芯片杂交; （e）分析反应的DNA微阵列芯片; 和（f）将来自步骤（e）分析的数据与对照组的表达程度进行比较。

公开(公告)号：KR1020070120709A

公开(公告)日：2007-12-26

申请号：KR1020060055307

申请日：2006-06-20

Applicant: 한국과학기술연구원

Inventor： 류재천 ,  김연정 ,  김미순

IPC: C12Q1/68 ,  G01N33/53

CPC classification number: C12Q1/6837 ,  G01N33/5308

Abstract: A biomarker is provided to be usefully used for monitoring and determining drugs or chemical materials having new nephrotoxicity and the danger of side effects, thereby being employed as a tool for investigating the action mode causing the nephrotoxicity. A biomarker for screening nephrotoxicity and side effect-inducing drugs comprises a gene selected from the group consisting of: Genebank XM_498383, Genebank AK001361, Genebank AJ002535, Genebank AK095253, Genebank AK026909, Genebank CR612719, Genebank NM_138417, Genebank CR601067, Genebank AK074052, Genebank XM_039495, Genebank BC067751, Genebank BM555452, Genebank AK025974, Genebank NM_152392, Genebank CR613579, Genebank NM_031298, Genebank BU673968, Genebank D67025, Genebank BC040191, Genebank XM_371569, Genebank NM_015946, Genebank BX537620, Genebank NM_005203, Genebank BC004102, Genebank AB075871, Genebank NM_005310, Genebank NM_173160, Genebank XM_499262, Genebank NM_080542, Genebank AY203954, Genebank NM_001005190, Genebank NM_005247, Genebank C033515, Genebank NM_000134, Genebank BC051890, Genebank Y008294, Genebank NM_014323, Genebank NM_001215, Genebank AY706204, Genebank NM_001004703, Genebank NM_002409, Genebank AK094580, Genebank NM_002918, Genebank AF097935, Genebank NM_012110, Genebank NM_001001524, Genebank BE889572, Genebank BX640959, Genebank AK122912, Genebank XM_495909, Genebank NM_020458, Genebank NM_145202, Genebank AF307451, Genebank AK024144, Genebank AB040955, Genebank AL833764, Genebank AY129013, Genebank AK096289, Genebank BM542003, Genebank AB018270, Genebank BC073828, Genebank AF279145, Genebank AY358993, Genebank BG167195, Genebank XM_498695, Genebank XM_373266, Genebank AK096265, Genebank XM_378608, Genebank AW575256, Genebank NM_001010867, Genebank AK023662, Genebank XM_290629, Genebank AL136662 and Genebank BC025741. The nephrotoxicity and side effect-inducing drug is selected from group consisting of amphotericin B, cisplatin and gentamicin.

Abstract translation: 提供了一种有效地用于监测和确定具有新的肾毒性和副作用危险的药物或化学物质的生物标志物，从而被用作调查引起肾毒性的作用模式的工具。 用于筛选肾毒性和副作用诱导药物的生物标志物包含选自以下的基因：Genebank XM_498383，Genebank AK001361，Genebank AJ002535，Genebank AK095253，Genebank AK026909，Genebank CR612719，Genebank NM_138417，Genebank CR601067，Genebank AK074052，Genebank XM_039495 ，Genebank BC067751，Genebank BM555452，Genebank AK025974，Genebank NM_152392，Genebank CR613579，Genebank NM_031298，Genebank BU673968，Genebank D67025，Genebank BC040191，Genebank XM_371569，Genebank NM_015946，Genebank BX537620，Genebank NM_005203，Genebank BC004102，Genebank AB075871，Genebank NM_005310，Genebank 使用Genebank NM_080542，Genebank NM_080542，Genebank NM_080542，Genebank NM_005247，Genebank NM_001005190，Genebank NM_005247，Genebank C033515，Genebank NM_000134，Genebank BC051890，Genebank Y008294，Genebank NM_014323，Genebank NM_001215，Genebank AY706204，Genebank NM_001004703，Genebank NM_002409，Genebank AK094580，Genebank NM_002918， Genebank AF097935，Genebank NM_01 2198，Genebank NM_001001524，Genebank NM_001001524，Genebank BE889572，Genebank BX640959，Genebank AK122912，Genebank XM_495909，Genebank NM_020458，Genebank NM_145202，Genebank AF307451，Genebank AK024144，Genebank AB040955，Genebank AL833764，Genebank AY129013，Genebank AK096289，Genebank BM542003，Genebank AB018270，Genebank BC073828， 基因库AF279145，Genebank AY358993，Genebank BG167195，Genebank XM_498695，Genebank XM_373266，Genebank AK096265，Genebank XM_378608，Genebank AW575256，Genebank NM_001010867，Genebank AK023662，Genebank XM_290629，Genebank AL136662和Genebank BC025741。 肾毒性和副作用诱导药物选自两性霉素B，顺铂和庆大霉素。

公开(公告)号：KR100748182B1

公开(公告)日：2007-08-10

申请号：KR1020050021718

申请日：2005-03-16

Applicant: 한국과학기술연구원

Inventor： 류재천 ,  김연정 ,  윤혜정

IPC: C12Q1/68

Abstract: 본 발명은 내분비계 장애물질(endocrine disrupting chemicals) 검색용 마커 유전자(marker genes) 및 이를 이용하여 내분비계 장애물질을 검색하는 방법에 관한 것이다. 본 발명의 검색 방법은 마이크로어레이(microarrary)를 통하여 선별된 에스트로겐 반응성 유전자들을 마커(marker)로 이용하여 새로운 내분비계 장애물질을 판정할 수 있으며, 검체의 내분비계 장애물질에 대한 노출 정도를 모니터링 하는데 유용하게 이용할 수 있다.

公开(公告)号：KR1020070033753A

公开(公告)日：2007-03-27

申请号：KR1020050088257

申请日：2005-09-22

Applicant: 한국과학기술연구원

Inventor： 류재천 ,  김연정

IPC: C12Q1/00

CPC classification number: C12Q1/6876 ,  C12Q2600/142 ,  C12Q2600/158 ,  G01N33/5058 ,  G01N33/68 ,  G01N2333/46

Abstract: 본 발명은 메틸수은(Methyl mercury) 노출 여부 진단용 바이오마커, 이를 이용하여 MeHg 노출을 진단하는 방법 및 MeHg에 대한 신경세포 보호제에 관한 것으로서, MeHg 노출시 발현 변화를 나타내는 SeW(selenoprotein W) 유전자 또는 단백질을 이용하면 MeHg의 노출 여부 진단에 유용하게 사용될 수 있으며, 글루타치온(GSH)은 MeHg의 독성으로부터 신경세포를 보호하는 신경세포 보호제로 이용될 수 있다.
Methyl mercury, neurotoxicity, Selenoprotein W

Abstract translation: 本发明是甲基汞（甲基汞）曝光是否诊断性生物标记，如通过使用它的神经细胞保护剂用于诊断甲基汞暴露和甲基汞，甲基汞缝（硒蛋白W）的基因或蛋白表达指示改变的方法，当暴露 如果使用可在无论是否曝光甲基汞，谷胱甘肽（GSH）的诊断是有用的，可以使用神经保护剂，以保护神经细胞从甲基汞的毒性。

公开(公告)号：KR1020060100077A

公开(公告)日：2006-09-20

申请号：KR1020050021718

申请日：2005-03-16

Applicant: 한국과학기술연구원

Inventor： 류재천 ,  김연정 ,  윤혜정

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12Q2600/156

Abstract: 본 발명은 내분비계 장애물질(endocrine disrupting chemicals) 검색용 마커 유전자(marker genes) 및 이를 이용하여 내분비계 장애물질을 검색하는 방법에 관한 것이다. 본 발명의 검색 방법은 마이크로어레이(microarrary)를 통하여 선별된 에스트로겐 반응성 유전자들을 마커(marker)로 이용하여 새로운 내분비계 장애물질을 판정할 수 있으며, 검체의 내분비계 장애물질에 대한 노출 정도를 모니터링 하는데 유용하게 이용할 수 있다.