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公开(公告)号:KR1020000066479A
公开(公告)日:2000-11-15
申请号:KR1019990013598
申请日:1999-04-16
Applicant: 한국과학기술연구원
IPC: C12P1/06
Abstract: PURPOSE: Provided is a method for purifying teicoplanin having antimicrobial activity. Thereby, teicoplanin is prepared easily in high yield by decreasing autotoxicity. CONSTITUTION: A method for purifying teicoplanin comprises the following steps of; (a) culturing teicoplanin producing strain; (b) performing hydrophobic adsorption chromatography of the culture broth, wherein the used resin is preferably selected from XAD-16, HP-20, silicagel or activated carbon; and (c) performing sugar-affinity chromatography of the culture broth, wherein concanvalin A, lentil lectin or wheat germ lectin is immobilized to the resin. Preferably, the producing teicoplanin strain is Actinoplanes teichomyceticus. The method further comprises step of adding neutral resin such as XAD-16. HP-20, silicagel or active carbon, to decrease autotoxicity phenomenon by teicoplanin.
Abstract translation: 目的:提供具有抗菌活性的替考拉宁纯化方法。 因此,通过降低自身毒性,可以容易地制备替考拉宁。 构成:提纯替考拉宁的方法包括以下步骤: (a)培养替考拉宁生产菌株; (b)进行培养液的疏水吸附色谱法,其中所用的树脂优选选自XAD-16,HP-20,硅胶或活性炭; 和(c)对所述培养液进行糖亲和层析,其中将所述血清白蛋白A,扁豆凝集素或小麦胚芽凝集素固定在树脂上。 优选地,生产替考拉宁菌株是鸟氨酸激酶。 该方法还包括加入中性树脂如XAD-16的步骤。 HP-20,硅胶或活性炭,以减少替考拉宁的自身毒性现象。
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公开(公告)号:KR1019970074925A
公开(公告)日:1997-12-10
申请号:KR1019960016630
申请日:1996-05-17
Applicant: 한국과학기술연구원
IPC: C12N1/14
Abstract: 본 발명은 신균주 페니실리움속(Penicillium sp.) F40362(기탁번호 : KCTC 0236BP) 이용한 미생물 제초제에 관한 것으로서, 더욱 상세하게는 크로버 및 유사잡초 병반으로부터 선발된 제초활성이 우수한 신균주 페니실리움속(Penicillium sp.) F40362 균이를 생물고분자물질, 미생물 안정제 및 농학적 허용 담체와 혼합하여 제조된 미생물 제초제에 관한 것이다.
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公开(公告)号:KR1019950014890B1
公开(公告)日:1995-12-16
申请号:KR1019930011381
申请日:1993-06-22
Applicant: 한국과학기술연구원
IPC: C05F11/08
Abstract: The preparation method for microbial fertilizer with mycorrhiza (I) consists of (1) mixing sand and vermiculite or volcanic soil as 1:1 and autoclaving to get sterilized soil (II), (2) laying (II) at 2cm depth, (3) innoculating 100 spores of (I), (4) laying (II) again at 2cm thick, (5) seeding on the surface-sterilized seeds (III) and cultivating (III) at sterilized condition for 3 months, (6) removing the above parts of (III), (7) grinding the remained parts of (III) and cultivated mycelia and spores of (I) to get 1 spore per gram of fertilizer and (8) drying. (I) is one or mixt. selected from Glomus sp., Fifaspora sp., Endogone sp., Sctellospora sp., Acaulospora sp., Sclerocystis sp., Entrophosporce sp., Glaziella sp. (III) is selected from Sudan grass, clover or kaolaing. This microbial fertilizer is useful for peanut or alfalfa cultivation.
Abstract translation: 微生物菌根化合物的制备方法(1)由(1)混合砂和蛭石或火山土为1:1,高压灭菌得到灭菌土(II),(2)铺设(II)2cm深度 )再次接种100个(I),(4)铺放(II)的孢子,(2)再沉淀2cm,(5)在灭菌的种子(III)上种植并培养(III)3个月,(6)去除 (III)的上述部分,(7)研磨(III)的剩余部分并培养(I)的菌丝体和孢子以获得每克肥料1孢子和(8)干燥。 (I)是一个或混合。 从Glomus sp。,Fifaspora sp。,Endogone sp。,Sctellospora sp。,Acaulospora sp。,Sclerocystis sp。,Entrophosporce sp。,Glaziella sp。 (三)选自苏丹草,三叶草或高粱。 这种微生物肥料可用于花生或苜蓿种植。
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