公开(公告)号：KR1020150132813A

公开(公告)日：2015-11-26

申请号：KR1020150155169

申请日：2015-11-05

Applicant: 한국과학기술원 ,  재단법인 지능형 바이오 시스템 설계 및 합성 연구단

Inventor： 김선창 ,  성봉현 ,  양경석 ,  이준형 ,  임기정 ,  박명근

IPC: C07K19/00 ,  C07K7/08 ,  C12N9/20 ,  C12N15/63

CPC classification number: C07K7/06 ,  C07K7/08 ,  C07K14/001 ,  C07K14/463 ,  C07K14/47 ,  C07K14/4723 ,  C07K2319/00 ,  C07K2319/21 ,  C12N9/20 ,  C12P7/649 ,  C12Y301/01003

Abstract: 본발명은리파아제활성이증대된양친매성펩타이드(amphipathic peptide) 및리파아제가연결된양친매성펩타이드-리파아제결합체, 상기결합체를코딩하는폴리뉴클레오티드, 상기폴리뉴클레오티드를포함하는발현벡터, 상기발현벡터가도입된형질전환체, 상기결합체의제조방법, 상기결합체를이용한지질의분해방법및 상기리파아제를이용한바이오디젤의생산방법에관한것이다.

Abstract translation: 本发明涉及具有改善的脂肪酶活性的两亲肽，与脂肪酶连接的两亲性肽 - 脂肪酶偶联物，编码缀合物的多核苷酸，包含多核苷酸的表达载体，引入表达载体的转化体， 缀合物，使用该缀合物的脂质分解方法，以及使用该脂肪酶的生物柴油的制备方法。

公开(公告)号：KR1020120062504A

公开(公告)日：2012-06-14

申请号：KR1020100123792

申请日：2010-12-06

Applicant: 한국과학기술원 ,  재단법인 지능형 바이오 시스템 설계 및 합성 연구단

Inventor： 김선창 ,  신주리 ,  임기정 ,  김다정 ,  이영웅 ,  장수아 ,  성봉현

IPC: C07K14/195 ,  C07K19/00 ,  C12N15/63 ,  A61P31/04

CPC classification number: A61K47/48776 ,  A61K38/16 ,  A61K47/65 ,  A61K47/6901 ,  C07K14/21 ,  C07K14/245 ,  C07K14/255 ,  C07K14/4723 ,  C07K2319/035 ,  C07K2319/50 ,  C12N15/62 ,  C12P21/02 ,  C12P21/06

Abstract: PURPOSE: A multimeric antibacterial peptide is provided to enable direct administration in vivo without isolation and purification of antibacterial peptides. CONSTITUTION: A multimeric antibacterial peptide expressed on cell surface contains one or more monomers cleaved by pepsin of formula 1(N terminal-[antibacterial peptide-pepsin cleavage amino acid linker]-C terminal) or formula 2(N terminal-[pepsin cleavage amino acid linker-antibacterial peptide]-C terminal). The antibacterial peptide contains the linker and other amino acids. The pepsin cleavage amino acid linker is an amino acid of leucine, phenyl alanine, or tyrosine. An antibacterial pharmaceutical composition contains the multimer as an active ingredient.

Abstract translation: 目的：提供多聚体抗菌肽以使得能够在体内直接施用而不分离和纯化抗菌肽。 构成：在细胞表面上表达的多聚体抗菌肽含有一种或多种由式1的胃蛋白酶切割的单体（N末端 - [抗菌肽 - 胃蛋白酶切割氨基酸接头] -C末端）或式2（N端 - [胃蛋白酶切割氨基 酸接头 - 抗菌肽] -C末端）。 抗菌肽含有接头和其他氨基酸。 胃蛋白酶切割氨基酸接头是亮氨酸，苯丙氨酸或酪氨酸的氨基酸。 抗菌药物组合物含有多聚体作为活性成分。

公开(公告)号：KR1020110130898A

公开(公告)日：2011-12-06

申请号：KR1020100050461

申请日：2010-05-28

Applicant: 한국과학기술원

Inventor： 김선창 ,  장수아 ,  김다정 ,  성봉현 ,  임기정 ,  신주리 ,  이영웅

IPC: A61K38/10 ,  A61K38/16 ,  A61P35/00

CPC classification number: C07K7/08 ,  A61K38/10 ,  G01N33/53 ,  G01N33/574

Abstract: PURPOSE: A composition containing peptides for preventing or treating cancer is provided to select cancer cell only and to ensure anticancer effect. CONSTITUTION: A composition for preventing or treating cancer contains peptides of amino acid sequence, APKAMX1LLX2LLX1LQKKGI. The peptide is denoted by amino acid sequence of sequence number 1 or 2. The composition additionally contains pharmaceutically acceptable carrier. The cancer is skin cancer, cervical cancer, lung cancer, or blood cancer.

Abstract translation: 目的：提供含有预防或治疗癌症的肽的组合物，仅选择癌细胞并确保抗癌作用。 构成：用于预防或治疗癌症的组合物含有氨基酸序列的肽，即ASCAMX1LLX2LLX1LQKKGI。 肽由序列号1或2的氨基酸序列表示。该组合物另外含有药学上可接受的载体。 癌症是皮肤癌，子宫颈癌，肺癌或血液癌。

公开(公告)号：KR1020030083324A

公开(公告)日：2003-10-30

申请号：KR1020020021811

申请日：2002-04-20

Applicant: 한국과학기술원

Inventor： 김선창 ,  성봉현 ,  유병조 ,  김정민 ,  이원식 ,  이충훈 ,  이준형

IPC: C12N15/00

CPC classification number: C12N15/1082 ,  C12N15/90

Abstract: PURPOSE: A transposon for bidirectional intramolecular genome deletions, a construction of a microorganism mutant and the identification of nonessential genes using the same transposon are provided, thereby effectively constructing the nonessential gene-removed Escherichia coli mutant. CONSTITUTION: Transposon TnRIBD sequentially comprises Tn5 transposase recognition site having the nucleotide sequence of SEQ ID NO: 2; tetR gene having the nucleotide sequence of SEQ ID NO: 4; Tnγδ transposase recognition site having the nucleotide sequence of SEQ ID NO: 3; CmR gene having the nucleotide sequence of SEQ ID NO: 5; Tnγδ transposase recognition site having the reverse complementary nucleotide sequence with SEQ ID NO: 3; sacB gene having the nucleotide sequence of SEQ ID NO: 6; and Tn5 transposase recognition site having the reverse complementary nucleotide sequence with SEQ ID NO: 2 in a direction of 5' to 3'. A construction method of a microorganism mutant comprises the steps of: constructing the transposon comprising the transposase recognition site and a selection marker; inserting the transposon into a certain site in the genome of a microorganism; and cloning a transposase expression vector into the genome of the microorganism to remove a portion of chromosome in both ends.

Abstract translation: 目的：提供用于双向分子内基因组缺失的转座子，构建微生物突变体和使用相同转座子鉴定非必需基因，从而有效构建非必需基因去除的大肠杆菌突变体。 构成：转座子TnRIBD依次包含具有SEQ ID NO：2的核苷酸序列的Tn5转座酶识别位点; 具有SEQ ID NO：4的核苷酸序列的tetR基因; 具有SEQ ID NO：3的核苷酸序列的Tnγδ转座酶识别位点; 具有SEQ ID NO：5的核苷酸序列的CmR基因; 具有与SEQ ID NO：3相反的互补核苷酸序列的Tnγδ转座酶识别位点; 具有SEQ ID NO：6的核苷酸序列的sacB基因; 和具有与SEQ ID NO：2相反的互补核苷酸序列的5'至3'的Tn5转座酶识别位点。 微生物突变体的构建方法包括以下步骤：构建包含转座酶识别位点和选择标记的转座子; 将转座子插入微生物基因组中的某个位点; 并将转座酶表达载体克隆到微生物的基因组中以去除两端染色体的一部分。