公开(公告)号：KR100467315B1

公开(公告)日：2005-01-24

申请号：KR1020010085038

申请日：2001-12-26

Applicant: 한국전자통신연구원

Inventor： 김민곤 ,  표현봉 ,  신용범 ,  박선희 ,  이상경 ,  신동호

IPC: G01N33/53

Abstract: PURPOSE: A biosensor using a competitive bond and a device and a method for detecting biological samples using the same are provided, thereby indirectly detecting the biological samples using the competitive bond, so that the signal sensitivity of the biosensor can be increased. CONSTITUTION: A biosensor capable of selectively binding with the analyte-binding material(ABM) comprises a board, a metal thin layer on the board, biological sample-like materials having the similar structure to the biological samples and immobilized on the metal thin layer, wherein the biological sample is glucose; the ABM is concanavalin A, coenzyme-removed apo-glucose oxidase or glucose dehydrogenase; the board is glass; and the metal thin layer is gold thin layer. A device(200) for detecting biological samples(62) comprises (a) the biosensor(100) containing the metal thin layer(20) and biological sample-like materials having similar structure to the biological samples and immobilized on the metal thin layer; (b) a buffer solution(110) containing the biological samples and ABM; (c) a dielectric medium(120) formed on both surfaces of the glass board; (d) refraction rate matching oil between the glass board and the dielectric medium; (e) a light emitting portion(140) for applying light to the glass board through the dielectric medium; and (f) a light receiving portion(150) for measuring the light intensity reflected from the glass board.

Abstract translation: 目的：提供使用竞争键的生物传感器和使用该生物传感器检测生物样品的装置和方法，由此通过竞争键间接检测生物样品，从而可以提高生物传感器的信号灵敏度。 构成：能够选择性地与分析物结合材料（ABM）结合的生物传感器包括板，板上的金属薄层，具有与生物样品类似结构并固定在金属薄层上的生物样品样材料， 其中生物样品是葡萄糖; ABM是伴刀豆球蛋白A，辅酶去除的脱辅基葡萄糖氧化酶或葡萄糖脱氢酶; 板子是玻璃的; 金属薄层为金薄层。 一种用于检测生物样品（62）的装置（200），包括：（a）包含金属薄层（20）的生物传感器（100）和具有与生物样品相似结构并固定在金属薄层上的生物样品样物质; （b）含有生物样品和ABM的缓冲溶液（110）; （c）在玻璃板的两个表面上形成的电介质（120）; （d）玻璃板和电介质之间的折射率匹配油; （e）发光部分（140），用于通过电介质向玻璃板施加光; 和（f）用于测量从玻璃板反射的光强度的光接收部分（150）。

公开(公告)号：KR100452946B1

公开(公告)日：2004-10-14

申请号：KR1020020074014

申请日：2002-11-26

Applicant: 한국전자통신연구원

Inventor： 이대식 ,  양해식 ,  고종수 ,  윤태환 ,  김효겸 ,  신용범 ,  김성진 ,  박세호 ,  김규원 ,  김윤태

IPC: C12M1/00 ,  G01N27/26

Abstract: PURPOSE: A low power consumption microfabricated thermal cycler and a method for fabrication of the same are provided, thereby accurately and minutely controlling the temperature of the reaction occurring area, so that it can be applied to various bio chips including PCR chip, protein chip, DNA chip, drug delivery system, micro biological/chemical reactor and lab-on-a-chip. CONSTITUTION: The low power consumption microfabricated thermal cycler comprises an upper board(112) and a lower board(100), wherein the upper board(112) comprises an fluid inlet(114) and outlet, a reaction chamber(118), and a fluid channel(116) connecting the inlet(114) and outlet to the reaction chamber(118); and the lower board(100) comprises an insulated heating thin layer(106) formed on the lower board(100), a heating means(102) formed on the insulated heating thin layer(106), a temperature sensor(104) formed on the insulated heating thin layer(106), and an insulating layer(108) covering the heating means(102) and the temperature sensor(104); and the insulated heating thin layer(106) is composed of Si3N4, SiO2, Si3N4/SiO2/Si3N4 or SiO2/Si3N4/SiO2 and has thickness of 0.1 to 10 micrometer.

Abstract translation: 目的：提供一种低功耗的微制造型热循环仪及其制造方法，从而准确而精确地控制反应发生区域的温度，从而可应用于各种生物芯片，包括PCR芯片，蛋白质芯片， DNA芯片，药物输送系统，微生物/化学反应器和芯片实验室。 构成：低功耗微制造热循环仪包括上板（112）和下板（100），其中上板（112）包括流体入口（114）和出口，反应室（118）和 将入口（114）和出口连接到反应室（118）的流体通道（116）; （100）上形成的绝缘加热薄层（106），形成在绝缘加热薄层（106）上的加热装置（102），形成在其上的温度传感器（104） 绝缘加热薄层（106）和覆盖加热装置（102）和温度传感器（104）的绝缘层（108）; 所述绝缘加热薄膜（106）由Si3N4，SiO2，Si3N4 / SiO2 / Si3N4或SiO2 / Si3N4 / SiO2组成，厚度为0.1〜10微米。

公开(公告)号：KR1020040039800A

公开(公告)日：2004-05-12

申请号：KR1020020068008

申请日：2002-11-05

Applicant: 한국전자통신연구원

Inventor： 박세호 ,  양해식 ,  이대식 ,  신용범 ,  김규원 ,  윤태환 ,  김성진 ,  김윤태

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q2537/143 ,  C12Q2527/113 ,  C12Q2527/101

Abstract: PURPOSE: A method for performing multiplex PCR with cyclic changes of PCR parameters is provided, thereby preventing production of primer dimer and non-specific product, and reducing the time for screening optimal multiplex PCR conditions. CONSTITUTION: A method for performing multiplex PCR from a sample comprises cyclic changes of PCR parameters including the maturation temperature and the elongation time of a primer, wherein the sample includes blood, blood plasma, circular DNA(vector), cDNA library, genome or cell containing the genome; the maturation temperature and elongation time of the primer are increased at regular intervals; the maturation temperature is increased according to the value of Tmax - Tmin per 1 cycle and the elongation time is increased according to the value of £(Lmax - Lmin) DNA polymerization rate of taq DNA polymerase(bp/sec)| (total cycle - 7), in which Lmax is the sequence size of a largest PCR product(bp) and Lmin is the sequence size of a smallest PCR product(bp); and the volume of the sample is less than 1 microliter.

Abstract translation: 目的：提供PCR参数循环变化进行多重PCR的方法，从而防止引物二聚体和非特异性产物的产生，并减少筛选最佳多重PCR条件的时间。 构成：从样品中进行多重PCR的方法包括PCR参数的循环变化，包括引物的成熟温度和延长时间，其中样品包括血液，血浆，环状DNA（载体），cDNA文库，基因组或细胞 含有基因组; 引物的成熟温度和延长时间以规则的间隔增加; 根据每1个循环的Tmax-Tmin的值增加成熟温度，并且根据taq DNA聚合酶（bp / sec）的£（Lmax-Lmin）DNA聚合速率的值增加延长时间| （总周期-7），其中Lmax是最大PCR产物（bp）的序列大小，Lmin是最小PCR产物（bp）的序列大小; 样品的体积小于1微升。

公开(公告)号：KR1020040039553A

公开(公告)日：2004-05-12

申请号：KR1020020067661

申请日：2002-11-02

Applicant: 한국전자통신연구원

Inventor： 표현봉 ,  신용범 ,  양해식 ,  김윤태

IPC: G01N24/00 ,  B82Y35/00

CPC classification number: G01N21/554

Abstract: PURPOSE: A localized surface plasma sensor and a fabricating method thereof are provided to measure a non-labeling sample characteristic in real-time, to adjust resonance absorbing conditions of a surface plasma, and to measure a sample through a transmission mode by using alignment of metal structures. CONSTITUTION: According to a localized surface plasma sensor using aligned and nano-sized metal structures and a fabricating method thereof, the localized surface plasma sensor is used as an immunosensor. The metal structure has vital materials such as antibody fixed to a surface of the metal structure(200). Also, the localized surface plasma sensor using the aligned and nano-sized metal structures includes an immunity sensor for measuring density of antigen specifically bound to the antibody. If the localized surface plasma sensor is used as a spectroelectrochemical sensor, a ligand(510) is fixed to the surface of the metal structure(200).

Abstract translation: 目的：提供局部表面等离子体传感器及其制造方法，以实时测量非标记样品特性，调节表面等离子体的共振吸收条件，并通过使用对准方式通过透射模式测量样品 金属结构。 构成：根据使用对准和纳米尺寸金属结构的局部表面等离子体传感器及其制造方法，局部表面等离子体传感器用作免疫传感器。 金属结构具有固定在金属结构（200）的表面上的重要材料，例如抗体。 此外，使用对准和纳米尺寸金属结构的局部表面等离子体传感器包括用于测量与抗体特异性结合的抗原的密度的免疫传感器。 如果局部表面等离子体传感器用作分光电化学传感器，则将配体（510）固定到金属结构（200）的表面。

公开(公告)号：KR100407821B1

公开(公告)日：2003-12-01

申请号：KR1020010073283

申请日：2001-11-23

Applicant: 한국전자통신연구원

Inventor： 신용범 ,  표현봉 ,  정지욱 ,  이상경 ,  신동호 ,  김민곤 ,  박선희

IPC: G01N21/27

CPC classification number: G01N21/648 ,  B82Y20/00 ,  G01N21/645 ,  G02B6/1226 ,  G02B6/134

Abstract: An active ion-doped waveguide-plasmon resonance (AID WPR) sensor based on plasmon surface resonance (PSR) and an imaging system using the sensor are provided. An additional dielectric thin film doped with active ions and acting as a waveguide is formed on a metal thin film. The active ions are excited by an incident light beam and fluoresce light of a shorter wavelength than the incident light beam through upconversion coupled to surface plasmon resonance, thereby increasing fluorescence intensity variations with respect to incident light angle variations. The AID WPR sensor and the imaging system can detect a minor refractive index variation of a sample, which could not be measured using an existing SPR sensor, or a trace adsorbed material, with 100 times larger refractive index resolution than the existing SPR sensor.

Abstract translation: 提供基于等离子体表面共振（PSR）的活性离子掺杂波导 - 等离子体共振（AID WPR）传感器和使用该传感器的成像系统。 掺杂有活性离子并用作波导的另外的电介质薄膜形成在金属薄膜上。 通过与表面等离子体共振耦合的上转换，活性离子被入射光束激发并发出比入射光束短的波长的光，由此相对于入射光角度变化增加荧光强度变化。 AID WPR传感器和成像系统可以检测样品的较小折射率变化，该变化无法使用现有SPR传感器或痕量吸附材料测量，其折射率分辨率比现有SPR传感器高100倍。

公开(公告)号：KR1020030042576A

公开(公告)日：2003-06-02

申请号：KR1020010073283

申请日：2001-11-23

Applicant: 한국전자통신연구원

Inventor： 신용범 ,  표현봉 ,  정지욱 ,  이상경 ,  신동호 ,  김민곤 ,  박선희

IPC: G01N21/27

CPC classification number: G01N21/648 ,  B82Y20/00 ,  G01N21/645 ,  G02B6/1226 ,  G02B6/134

Abstract: PURPOSE: A waveguide-plasmon resonance sensor and an image system are provided to improve sensitivity of the waveguide-plasmon resonance sensor by coating a dielectric thin film doped with active ions on a metal layer as a waveguide. CONSTITUTION: An active ion doped waveguide-plasmon resonance sensor includes a conductive film(122) for providing surface plasmon and a dielectric medium(110) aligned at one side of the conductive film(122). A light source(130) radiates incident light, which is incident into the conductive film(122) through the dielectric medium(110). A dielectric film is coated on the other side of the conductive film(122). A sample(123) is attached to the dielectric film. Active ions(171) are doped into the dielectric film. Active ions(171) are excited by incident light(131), thereby generating fluorescent light(139). A light receiving section(140) is provided to measure intensity of fluorescent light(139).

Abstract translation: 目的：提供波导 - 等离子体共振传感器和图像系统，以通过在金属层上涂覆掺杂有活性离子的介电薄膜作为波导来提高波导 - 等离子体共振传感器的灵敏度。 构成：主动离子掺杂波导 - 等离子体共振传感器包括用于提供表面等离子体激元的导电膜（122）和在导电膜（122）的一侧对准的电介质（110）。 光源（130）通过电介质（110）辐射入射到导电膜（122）中的入射光。 在导电膜（122）的另一侧上涂覆有电介质膜。 样品（123）附着在电介质膜上。 活性离子（171）被掺杂到电介质膜中。 活性离子（171）被入射光（131）激发，从而产生荧光（139）。 提供光接收部分（140）以测量荧光的强度（139）。

公开(公告)号：KR1020020015414A

公开(公告)日：2002-02-28

申请号：KR1020000048504

申请日：2000-08-22

Applicant: 한국전자통신연구원

Inventor： 최용규 ,  신용범 ,  조두희 ,  김경헌

IPC: C03B37/012

CPC classification number: C03B37/01268

Abstract: PURPOSE: Provided is a clad mold for producing optical fiber by a melting method which can be prepared economically in a simple process, and thereby it is possible to prevent a parent material of optical fiber from being contaminated by impurities and deteriorated in its quality. CONSTITUTION: The mold of clad(10) for producing a parent material of optical fiber comprises a clad mold(11) of a pipe shape having an end closed, a predetermined length and an inner diameter corresponding to a diameter of a clad; and a cylindrical core rod(12) attached to the closed end of the clad mold(11) in the same axis as the clad mold(11) and having a predetermined length and an outer diameter corresponding to a diameter of a core to be formed. The clad mold is prepared by (i) pouring melted glass to a hollow of the mold of clad(10), followed by solidifying the glass to form a clad; (ii) removing the clad mold(11); (iii) removing the core rod(12); and (iv) inserting a core rod into a hollow formed by removing the core rod in the step (iii).

Abstract translation: 目的：提供一种通过熔融法生产光纤的复合模具，其可以在简单的工艺中经济地制备，从而可以防止光纤的母材被杂质污染并且质量劣化。 构成：用于制造光纤母材的包层（10）的模具包括具有端部封闭，预定长度和对应于包层直径的内径的管状包层模具（11） 以及圆筒形芯棒（12），其与所述包层模具（11）在与所述包层模具（11）相同的轴线上的所述包覆模具（11）的所述封闭端部附接，并且具有预定的长度和对应于待形成的芯的直径的外径 。 包层模具通过以下步骤制备：（i）将熔融的玻璃浇注到包层（10）的模具的中空，然后固化玻璃以形成包层; （ii）移除包层模具（11）; （iii）移除芯棒（12）; 和（iv）将芯棒插入到通过在步骤（iii）中去除芯棒而形成的中空部中。