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21.发明公开개미산을 이용해 수소를 생산해 낼 수 있는 Thermococcus spp. 로부터 분리된 신규한 서열번호 2의 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 无效序列号2的新型氢化物，其从热能SPP中纯化。 通过使用FORMATE，编码它们的基因，以及使用具有基因的微生物生产氢的方法
公开(公告)号：KR1020140022081A
公开(公告)日：2014-02-21
申请号：KR1020137034429
申请日：2009-09-07
Applicant: 한국해양과학기술원
CPC classification number: C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52
Abstract: The present invention relates to novel hydrogenases separated from new hyperthermophilic strains belonging to Thermococcus genus, genes encoding the same, and a method for producing hydrogen using the same. According to a method for producing hydrogen of the present invention, a large amount of hydrogen can be produced by culturing the strains in a specific culture condition and therefore, it is more economical and effective compared to the existing method for producing hydrogen and hydrogen can be produced at a high temperature.
Abstract translation: 本发明涉及从属于Thermococcus属的新的超嗜热菌株分离出的新型氢化酶，以及使用该氢过氧化氢的方法。 根据本发明的氢的制造方法，可以通过在特定培养条件下培养菌株来生产大量氢，因此与现有的氢气制备方法相比，其更经济有效，氢可以是 在高温下生产。
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公开(公告)号：KR1020130115496A
公开(公告)日：2013-10-22
申请号：KR1020120037823
申请日：2012-04-12
Applicant: 한국해양과학기술원
Abstract: PURPOSE: A carbon monoxide dissolution device for producing hydrogen using anaerobic microorganisms is provided to help hydrogen-producing anaerobic microorganisms to effectively use carbon monoxides when hydrogen is produced, thereby enhancing hydrogen production efficiency. CONSTITUTION: A carbon monoxide dissolution device (100) for producing hydrogen using anaerobic microorganisms comprises: a main body (110) with an opening (111); tube cassettes (120) which have a tube for allowing a culture medium containing hydrogen-producing anaerobic microorganisms and carbon monoxides to pass; and a fitting member (130) which provides a delayed path for delaying time for dissolving the carbon monoxides. The tubes have supply ports (123) and outlets (124) by connecting the tubes between the tube cassettes. The both ends of the tubes are exposed to the outside to help the tube to be connected to other tubes. [Reference numerals] (230) Carbon monoxide supplying unit; (240) Hydrogen collecting unit; (250) Cooling unit; (260) Culture medium supplying unit
Abstract translation: 目的：提供一种使用厌氧微生物生产氢气的一氧化碳溶解装置，用于在生产氢气时帮助产氢厌氧微生物有效地使用一氧化碳，从而提高氢生产效率。 构成：使用厌氧微生物生产氢的一氧化碳溶解装置（100）包括：具有开口（111）的主体（110）; 管筒（120）具有用于允许含有产氢厌氧微生物和一氧化碳的培养基通过的管; 以及装配构件（130），其提供用于延迟用于溶解一氧化碳的时间的延迟路径。 管通过将管连接在管盒之间而具有供给口（123）和出口（124）。 管的两端暴露于外部，以帮助管连接到其他管。 （附图标记）（230）一氧化碳供给单元; （240）集氢单元; （250）冷却单元; （260）培养基供应单元
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公开(公告)号：KR1020110126012A
公开(公告)日：2011-11-22
申请号：KR1020100045692
申请日：2010-05-14
Applicant: 한국해양과학기술원
Abstract: PURPOSE: A DNA polymerase F7 and genes thereof are provided to enhance elongation and processivity and to be used in PCR. CONSTITUTION: A DNA polymerase F7 contains an amino acid sequence of sequence number 2. A DNA polymerase F7 gene encoding an amino acid sequence of sequence number 2 contains a base of sequence number 1. A recombinant vector contains the polymerase F7 gene of sequence number 1. A host cell is transformed with the recombinant vector containing the DNA polymerase F7 gene.
Abstract translation: 目的：提供DNA聚合酶F7及其基因以增加伸长和持续性，并用于PCR。 构成：DNA聚合酶F7含有序列号2的氨基酸序列。编码序列号2的氨基酸序列的DNA聚合酶F7基因含有序列号1的碱基。重组载体含有序列号1的聚合酶F7基因 用含有DNA聚合酶F7基因的重组载体转化宿主细胞。
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24.发明公开일산화탄소를 이용해 수소를 생산해 낼 수 있는 Ｔｈｅｒｍｏｃｃｕｓ ｓｐｐ．로부터 분리된 신규한 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 无效来自热能SPP的新型氢化物2。 通过使用碳黑单体，编码它们的基因，以及使用具有基因的微生物生产氢的方法
公开(公告)号：KR1020110094092A
公开(公告)日：2011-08-19
申请号：KR1020117014737
申请日：2009-09-07
Applicant: 한국해양과학기술원
CPC classification number: C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52
Abstract: PURPOSE: A novel hydrogenase isolated from Thermococcus spp. and a method for producing hydrogen using the same are provided to produce hydrogen without high temperature and pressure. CONSTITUTION: A hydrogenase contains an amino acid sequence of sequence number 5. A gene encoding the hydrogenase contains a base sequence of sequence number 16. A method for producing hydrogen from Thermococcus spp. comprises: a step of preparing a medium; a step of culturing Thermococcus spp. in the medium; and a step of isolating hydrogen. The medium contains carbon monoxide. The Thermococcus spp. is Thermococcus onnurineus NA1(deposit number: KCTC 10859BP).
Abstract translation: 目的：从Thermococcus spp。分离出的新型氢化酶。 并且提供使用其制造氢的方法以在没有高温和高压的情况下产生氢。 构成：氢化酶含有序列号5的氨基酸序列。编码氢化酶的基因含有序列号16的碱基序列。从Thermococcus spp。生产氢的方法。 包括：制备介质的步骤; 培养热球菌属的一个步骤。 在媒体中 和分离氢的步骤。 介质含有一氧化碳。 热球菌属 是Thermococcus onnurineus NA1（保藏号：KCTC 10859BP）。
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25.发明公开개미산을 이용해 수소를 생산해 낼 수 있는 Ｔｈｅｒｍｏｃｃｕｓ ｓｐｐ．로부터 분리된 신규한 서열번호 7의 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 无效序列号7的新型氢化物，从热能SPP中纯化。 通过使用FORMATE，编码它们的基因，以及使用具有基因的微生物生产氢的方法
公开(公告)号：KR1020110093912A
公开(公告)日：2011-08-18
申请号：KR1020117014736
申请日：2009-09-07
Applicant: 한국해양과학기술원
CPC classification number: C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52
Abstract: PURPOSE: A method for producing hydrogen using hydrogenase isolated from Thermococcus spp. is provided to cheaply and efficiently produce hydrogen even at high temperature. CONSTITUTION: A hydrogenase contains an amino acid sequence of sequence number 7. A gene encoding the hydrogenase contains a base sequence of sequence number 18. A method for producing hydrogen from Thermococcus spp. comprises: a step of preparing a medium; a step of culturing Thermococcus spp. in the medium at 80°C under the anaerobic condition; and a step of isolating hydrogen. The Thermococcus spp. is Thermococcus onnurineus NA1(deposit number: KCTC 10859BP). The medium contains formate.
Abstract translation: 目的：使用从Thermococcus spp分离的氢化酶产生氢的方法。 即使在高温下也能廉价高效地生产氢。 构成：氢化酶含有序列号7的氨基酸序列。编码氢化酶的基因含有序列号18的碱基序列。一种从Thermococcus spp。生产氢的方法。 包括：制备介质的步骤; 培养热球菌属的一个步骤。 在80℃的培养基中，在厌氧条件下; 和分离氢的步骤。 热球菌属 是Thermococcus onnurineus NA1（保藏号：KCTC 10859BP）。 培养基含有甲酸。
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Patent Agency Ranking26.发明公开Ｔｈｅｒｍｏｃｏｃｃｕｓ ｓｐｐ.로부터 분리된 신규한 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 审中-实审从热精球菌中纯化的新型氢化物，编码基因的方法以及使用具有基因的微生物生产氢的方法
公开(公告)号：KR1020110069744A
公开(公告)日：2011-06-23
申请号：KR1020107013071
申请日：2009-09-07
Applicant: 한국해양과학기술원
CPC classification number: C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52 , C12Y112/01004
Abstract: PURPOSE: A novel hydrogenase isolated from Thermococcus sp. and a method for producing hydrogen using the same are provided to produce a large amount of hydrogen even at high temperature. CONSTITUTION: A hydrogenase has an amino acid sequence selected from sequence numbers 1-8. A gene encoding the hydrogenase contains a base sequence selected from sequence numbers 12-19. A method for producing hydrogen from Thermococcus spp. comprises: a step of preparing a medium; a step of culturing Thermococcus spp. in a culture container; and a step of isolating hydrogen from culture container. The Thermococcus spp. is Thermococcus onnurineus NA1(deposit number: KCTC 10859BP). The medium contains carbon monoxide, alkyl formate, or starch. The culture is performed at 80°C and under anaerobic condition.
Abstract translation: 目的：从Thermococcus sp。 提供使用其制造氢的方法，即使在高温下也能产生大量的氢。 构成：氢化酶具有选自序列号1-8的氨基酸序列。 编码氢化酶的基因含有选自序列号12-19的碱基序列。 一种从Thermococcus spp。生产氢的方法 包括：制备介质的步骤; 培养热球菌属的一个步骤。 在文化容器中 以及从培养容器中分离氢的步骤。 热球菌属 是Thermococcus onnurineus NA1（保藏号：KCTC 10859BP）。 培养基含有一氧化碳，甲酸烷基酯或淀粉。 培养在80℃和无氧条件下进行。
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公开(公告)号：KR1020110061837A
公开(公告)日：2011-06-10
申请号：KR1020090118351
申请日：2009-12-02
Applicant: 한국해양과학기술원
CPC classification number: C12N9/14 , C12N15/67 , C12Y303/02009
Abstract: PURPOSE: A novel epoxide hydrolase is provided to hydrolyze non-degradable epoxide and to attenuate SO cytotoxicity. CONSTITUTION: An expoxide hydrolase has an amino acid sequence of sequence number 1. A method for preparing the epoxide hydrolase comprises: a step of inserting a gene encoding the epoxide hydrolase to an expression vector to prepare a recombinant vector; a step of introducing the recombinant vector to a host cell for overexpression; and a step of isolating and purifying the overexpressed apoxide hydrolase. The epoxy substrate is non-degradable epoxide substrate, styrene 7,8-oxide.
Abstract translation: 目的：提供一种新的环氧化物水解酶来水解不可降解的环氧化物并减弱SO细胞毒性。 一种环氧化物水解酶具有序列号1的氨基酸序列。制备环氧化物水解酶的方法包括：将编码环氧化物水解酶的基因插入表达载体以制备重组载体的步骤; 将重组载体导入宿主细胞以进行过表达的步骤; 以及分离和纯化过表达的过氧化物水解酶的步骤。 环氧基材是不可降解的环氧化物底物，苯乙烯7,8-氧化物。
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28.发明授权개미산을 이용해 수소를 생산해 낼 수 있는 Thermococcus spp. 로부터 분리된 신규한 서열번호 7의 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 有权可用甲酸生产氢气的Thermococcus spp。 使用序列号7的新型氢化酶，编码它的基因和具有该基因的微生物制造氢的方法
公开(公告)号：KR101736482B1
公开(公告)日：2017-05-30
申请号：KR1020137034430
申请日：2009-09-07
Applicant: 한국해양과학기술원
CPC classification number: C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52
Abstract: 본발명은속에속하는고호열성신균주로부터분리한신규한수소화효소(hydrogenase), 이를암호화하는유전자및 이들을이용하여수소를생산하는방법에관한것이다. 본발명의수소생산방법에따르면상기균주를특정배양조건으로배양하는것만으로많은양의수소를생산할수 있으므로, 기존의수소생산방법에비하여경제적이고효율적이며, 고온에서도수소를생산할수 있는장점이있다.
Abstract translation: 本发明涉及一种新的氢化酶，编码该氢化酶的基因，以及使用该氢化酶生产氢的方法。 由于根据本发明的制氢方法的应变可产生具有只培养haneungeot到特定的培养条件，以及经济和比现有的制氢方法高效，存在能够即使在高温下产生氢的优点大量氢的。
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29.发明公开
公开(公告)号：KR1020160114654A
公开(公告)日：2016-10-05
申请号：KR1020167023473
申请日：2009-09-07
Applicant: 한국해양과학기술원
CPC classification number: C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52 , C12Y112/01004
Abstract: 수소화효소(hydrogenase), 이를암호화하는유전자및 이들을이용하여수소를생산하는방법에관한것이다. 본발명의수소생산방법에따르면상기균주를특정배양조건으로배양하는것만으로많은양의수소를생산할수 있으므로, 기존의수소생산방법에비하여경제적이고효율적이며, 고온에서도수소를생산할수 있는장점이있다.
Abstract translation: 本发明涉及从属于Thermococcus spp的新型超嗜热菌株分离的新型氢化酶，编码氢化酶的基因，以及使用具有该基因的菌株产生氢的方法。 根据本发明的氢生产方法，仅通过在特定培养条件下培养菌株可以产生大量的氢。 因此，本发明的方法的优点在于它们比现有的氢气生产方法更经济和有效，并且即使在高温下也可以产生氢。
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公开(公告)号：KR101534483B1
公开(公告)日：2015-07-07
申请号：KR1020130137330
申请日：2013-11-13
Applicant: 한국해양과학기술원
Abstract: 본발명은써모코커스온누리누스 WTC155T(WTC155T, 기탁번호: KCTC12414BP) 균주에관한것이다. 또한, (a) 제 1 항의균주에일산화탄소를공급하여배양하는단계; 및 (b) 상기배양물로부터수소를분리하는단계를포함하는것을특징으로하는수소생산방법에관한것이다. 본발명에따른균주는고농도의일산화탄소에서적응한균주로서 0.2 내지 0.4 vvm의일산화탄소에서도생장속도및 수소생산능력이증가하였다.
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