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公开(公告)号：KR1020120129851A
公开(公告)日：2012-11-28
申请号：KR1020120116121
申请日：2012-10-18
Applicant: 한국해양과학기술원
Abstract: PURPOSE: An esterase KTL7 which is separated from the bottom of the sea is provided to enhance activities and to have stability in wide range of temperature. CONSTITUTION: An esterase KTL7 which is separated from the bottom of the sea has amino acid sequence of the sequence number 32(SEQ ID NO:32). A gene ciphering the esterase has the base sequence described in the sequence number 31. A manufacturing method of the esterase gene comprises the following steps: transforming the cells into recombinant vector which includes the esterase of the sequence number 31; culturing the transformed cells; and separating the esterase from the cultured cell. The novel esterase gene is obtained by the following steps: extracting environmental DNA from the deposit of the depth of 1,400 M; building a library by using fosmid vector; and separating the gene from a clone which is active in the tricaprulin flat medium.
Abstract translation: 目的：提供与海底分离的酯酶KTL7，以增强活性，并在宽温度范围内具有稳定性。 构成：从海底分离出的酯酶KTL7具有序列号32（SEQ ID NO：32）的氨基酸序列。 加密酯酶的基因具有序列号31中描述的碱基序列。酯酶基因的制备方法包括以下步骤：将细胞转化成包含序列号31的酯酶的重组载体; 培养转化细胞; 并从培养的细胞中分离酯酶。 通过以下步骤获得新型酯酶基因：从1400m深度的沉积物中提取环境DNA; 使用fosmid载体构建库; 并将该基因与三权纲平台培养基中活性的克隆分离。
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公开(公告)号：KR1020110126009A
公开(公告)日：2011-11-22
申请号：KR1020100045689
申请日：2010-05-14
Applicant: 한국해양과학기술원
Abstract: PURPOSE: A DNA polymerase P7 and genes thereof are provided to ensure excellent processivity, fidelity, and elongation length performance. CONSTITUTION: A DNA polymerase P7 contains an amino acid sequence of sequence number 2. A DNA polymerase P7 gene encodes an amino acid sequence of sequence number 2. The DNA polymerase P7 gene contains a base sequence of sequence number 1. A recombinant vector contains the P7 gene. A host cell is transformed with the recombinant vector containing the P7 gene. The DNA polymerase P7 is derived from Thermococcus sp.
Abstract translation: 目的：提供DNA聚合酶P7及其基因以确保优异的持续性，保真度和伸长长度性能。 构成：DNA聚合酶P7含有序列号2的氨基酸序列.DNA聚合酶P7基因编码序列号2的氨基酸序列.DNA聚合酶P7基因含有序列号1的碱基序列。重组载体含有 P7基因。 用含有P7基因的重组载体转化宿主细胞。 DNA聚合酶P7衍生自Thermococcus sp。
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23.发明公开개미산을 이용해 수소를 생산해 낼 수 있는 Ｔｈｅｒｍｏｃｃｕｓ ｓｐｐ．로부터 분리된 신규한 서열번호 2의 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 无效序列号2的新型氢化物，其从热能SPP中纯化。 通过使用FORMATE，编码它们的基因，以及使用具有基因的微生物生产氢的方法
公开(公告)号：KR1020110093913A
公开(公告)日：2011-08-18
申请号：KR1020117014743
申请日：2009-09-07
Applicant: 한국해양과학기술원
CPC classification number: C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52
Abstract: PURPOSE: A hydrogenase isolated from Thermococcus spp. is provided to generate hydrogen at room temperature and pressure without harmful by-products. CONSTITUTION: A hydrogenase contains an amino acid sequence of sequence number 2. A gene encoding the hydrogenase contains a base sequence of sequence number 13. A method for producing hydrogen from Thermococcus spp. comprises: a step of culturing Thermococcus spp. in a medium containing formate at 80°C under the anaerobic condition; and a step of isolating hydrogen from a culture container.
Abstract translation: 目的：从Thermococcus spp。分离的氢化酶。 被提供以在室温和压力下产生氢气而没有有害的副产物。 构成：氢化酶含有序列号2的氨基酸序列。编码氢化酶的基因含有序列号13的碱基序列。从Thermococcus spp。生产氢的方法。 包括：培养Thermococcus spp的步骤。 在含有甲酸盐的培养基中，在80℃，无氧条件下， 以及从培养容器中分离氢的步骤。
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公开(公告)号：KR1020160111732A
公开(公告)日：2016-09-27
申请号：KR1020150036776
申请日：2015-03-17
Applicant: 한국해양과학기술원
Abstract: 본발명은속균주를사용하여탄소질원료를가스화한합성가스로부터생촉매를제조하는방법에관한것이다. 본발명의제조방법을통하여탄소질원료로부터유용한생촉매를대량으로생산할수 있어경제성을확보할수 있으며, 별도의유해한부산물을발생시키지도아니한다.
Abstract translation: 本发明涉及一种生物催化剂的制备方法，该方法由使用属于热球菌属的菌株气化原始碳质材料制成的合成气体。 根据本发明，通过本发明的制造方法可以从原料碳质材料大量生产有用的生物催化剂，从而确保经济可行性。 此外，本发明的方法也不会产生另外的有害副产物。
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公开(公告)号：KR1020150055243A
公开(公告)日：2015-05-21
申请号：KR1020130137330
申请日：2013-11-13
Applicant: 한국해양과학기술원
Abstract: 본발명은써모코커스온누리누스 WTC155T(WTC155T, 기탁번호: KCTC12414BP) 균주에관한것이다. 또한, (a) 제 1 항의균주에일산화탄소를공급하여배양하는단계; 및 (b) 상기배양물로부터수소를분리하는단계를포함하는것을특징으로하는수소생산방법에관한것이다. 본발명에따른균주는고농도의일산화탄소에서적응한균주로서 0.2 내지 0.4 vvm의일산화탄소에서도생장속도및 수소생산능력이증가하였다.
Abstract translation: 本发明涉及Thermococcusonnineine WTC155T菌株（登录号：KCTC12414BP）和使用该菌株的制氢方法。 制氢方法包括以下步骤：（a）向菌株提供一氧化碳以培养菌株; 和（b）从培养物中分离出氢。 根据本发明，菌株可以适应高浓度的一氧化碳，并且增加一氧化碳浓度为0.2至0.4vvm的生长速率和生产能力。
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Patent Agency Ranking
公开(公告)号：KR101401603B1
公开(公告)日：2014-06-27
申请号：KR1020120065303
申请日：2012-06-19
Applicant: 한국해양과학기술원
CPC classification number: C12N15/67 , C07K14/195 , C12N9/0008 , C12P3/00
Abstract: 본 발명은 수소생산능이 증가된 써모코코스 온누리우스 엔에이원 돌연변이 균주 및 이를 이용한 수소생산방법에 관한 것으로, 보다 구체적으로는 CO 디하이드로게나아제, 하이드로게나아제(
mch ) 및 복합-구조체 Na
+ /H
+ 안티포터(antiporter) (
mnh3 )유전자 클러스터의 발현율이 증가된 것을 특징으로 하는 수소생산능력이 증가된
Thermococcus
onnurineus NA1 및 이를 이용한 수소 생산방법에 관한 것이다.-
公开(公告)号：KR101383546B1
公开(公告)日：2014-04-10
申请号：KR1020070104219
申请日：2007-10-16
Applicant: 한국해양과학기술원
Abstract: 본 발명은 심해 해저에서 분리된 리파제 및 에스터라제 효소 및 이의 제조방법에 관한 것으로서, 서남태평양 해역(남위 3°89′, 동경 152°49′) 깊이 1,400 미터에 이르는 심해 퇴적물, 북극 다산기지 주변 연안 퇴적물, 강화도 갯벌 퇴적물로부터 구축된 메타게놈 라이브러리를 대상으로 분리한 신규 리파제 및 에스터라제 효소, 이들을 암호화 하는 유전자 및 리파제 및 에스터라제 효소 생산방법을 제공한다.
리파제, 에스터라제-
公开(公告)号：KR1020130142251A
公开(公告)日：2013-12-30
申请号：KR1020120065303
申请日：2012-06-19
Applicant: 한국해양과학기술원
CPC classification number: C12N15/67 , C07K14/195 , C12N9/0008 , C12P3/00
Abstract: The present invention relates to a mutated thermococcus onnurineus NA1 with increased hydrogen productivity and a method for producing hydrogen using the same, and more specifically, to a mutated thermococcus onnurineus NA1 with increased hydrogen productivity and a method for producing hydrogen using the same, wherein the expression of a CO dehydrogenase, a hydrogenase (mch), a complex-structure Na+/H+ antiporter(mnh3) gene cluster is improved.
Abstract translation: 本发明涉及具有增加的氢生产率的突变型热带炎热球菌NA1和使用其的氢的制造方法，更具体地说，涉及具有增加的氢生产率的突变的on鱼属NA1和使用其的氢的制造方法，其中 CO脱氢酶，氢化酶（mch），复合结构Na + / H +反转录酶（mnh3）基因簇的表达得到改善。
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公开(公告)号：KR1020130115495A
公开(公告)日：2013-10-22
申请号：KR1020120037822
申请日：2012-04-12
Applicant: 한국해양과학기술원
CPC classification number: C12M21/04 , C01B3/02 , C12M23/36 , C12M23/42 , C12M23/58 , C12M29/26 , C12M41/12
Abstract: PURPOSE: A hydrogen producing device using anaerobic microorganisms is provided to enhance hydrogen production efficiency to allow hydrogen-producing anaerobic microorganisms to use carbon monoxides when hydrogen is produced. CONSTITUTION: A hydrogen producing device (100) using anaerobic microorganisms comprises: a culture machine (110) which stores a culture medium containing hydrogen-producing anaerobic microorganisms; a circulation line (120) which is connected to the culture machine to discharge the culture medium from the culture machine and has a pump (121) for circulation of the culture medium; a carbon monoxide supply unit (130) which supplies carbon monoxides to the culture medium; a dissolution unit (140) with a tube which provides a delayed path for delaying time for dissolving the carbon monoxides; and a hydrogen collection unit (150) which captures hydrogen from gas separated from the culture medium. [Reference numerals] (130) Carbon monoxide supplying unit; (148) Control unit; (150) Hydrogen collecting unit; (160) Cooling unit; (170) Culture medium supplying unit
Abstract translation: 目的：提供使用厌氧微生物的氢气生产装置，以提高制氢效率，使氢气产生厌氧微生物在生产氢气时使用一氧化碳。 构成：使用厌氧微生物的制氢装置（100）包括：储存含有产氢厌氧微生物的培养基的培养机（110） 与培养机连接以从培养机排出培养基的循环管线（120），并具有用于培养培养基循环的泵（121）; 向培养基供给一氧化碳的一氧化碳供给单元（130） 溶解单元（140），其具有提供用于延迟溶解所述一氧化碳的时间的延迟路径的管; 和从所述培养基分离的气体捕获氢的氢气收集单元（150）。 （附图标记）（130）一氧化碳供给单元; （148）控制单元; （150）集氢单元; （160）冷却单元; （170）培养基供给单元
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30.发明授权일산화탄소를 이용해 수소를 생산해 낼 수 있는 Thermococcus spp. 로부터 분리된 신규한 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 有权Thermococcus spp。可用一氧化碳生产氢气。 使用新型氢化酶，编码其的基因和具有该基因的微生物生产氢的方法
公开(公告)号：KR101736484B1
公开(公告)日：2017-05-30
申请号：KR1020137034431
申请日：2009-09-07
Applicant: 한국해양과학기술원
CPC classification number: C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52
Abstract: 본발명은속에속하는고호열성신균주로부터분리한신규한수소화효소(hydrogenase), 이를암호화하는유전자및 이들을이용하여수소를생산하는방법에관한것이다. 본발명의수소생산방법에따르면상기균주를특정배양조건으로배양하는것만으로많은양의수소를생산할수 있으므로, 기존의수소생산방법에비하여경제적이고효율적이며, 고온에서도수소를생산할수 있는장점이있다.
Abstract translation: 本发明涉及一种新的氢化酶，编码该氢化酶的基因，以及使用该氢化酶生产氢的方法。 由于根据本发明的制氢方法的应变可产生具有只培养haneungeot到特定的培养条件，以及经济和比现有的制氢方法高效，存在能够即使在高温下产生氢的优点大量氢的。
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