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公开(公告)号：KR1020090038757A
公开(公告)日：2009-04-21
申请号：KR1020070104219
申请日：2007-10-16
Applicant: 한국해양과학기술원
Abstract: Provided is lipase and esterase isolated from deep sea mass-produced by genes coding the lipase and esterase. An esterase gene codes esterase having amino acid sequence of SEQ ID:NO 2, 4, 6, 10, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32 or 34. A method for preparing the esterase comprises the following steps of: cultivating a transformed cell with a recombinant vector; and isolating the esterase from the cultivated cells. The recombinant vector includes sequence number of 1, 3, 5, 9, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31 or 33. The lipase gene codes lipase having amino acid sequence of SEQ ID:NO 8 or 12. The method for preparing the lipase consists of the following steps of: cultivating a transformed cell with a recombinant vector; and isolating the lipase from the cultivated cells. The recombinant vector has sequence number of 7 or 11.
Abstract translation: 提供从通过编码脂肪酶和酯酶的基因产生的深海分离的脂肪酶和酯酶。 酯酶基因编码具有SEQ ID NO：2,4,6,10,14,16,18,20,22,24,26,28,30,32或34的氨基酸序列的酯酶。制备酯酶的方法 包括以下步骤：用重组载体培养转化的细胞; 并从培养的细胞中分离酯酶。 重组载体包含序列号1,3,5,9,13,15,17,19,21,23,25,27,29,31或33.脂肪酶基因编码具有SEQ ID NO：1的氨基酸序列的脂肪酶， NO 8或12.制备脂肪酶的方法包括以下步骤：用重组载体培养转化的细胞; 并从培养的细胞中分离脂肪酶。 重组载体的序列号为7或11。
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公开(公告)号：KR101453012B1
公开(公告)日：2014-10-21
申请号：KR1020120105557
申请日：2012-09-24
Applicant: 한국해양과학기술원 , 대한민국(농촌진흥청장)
Abstract: 본 발명은 신규의 에스터라제 및 이를 암호화하는 유전자, 및 이를 이용한 에스터라제 생산방법에 관한 것으로, 본 발명에 따른 에스터라제는 고온에서도 높은 활성을 나타내고, 높은 pH에서도 에스터라제 활성이 있다. 따라서 본 발명에 따른 에스터라제는 세제, 식품 및 의약품 생산에 유용하게 활용될 수 있다.
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公开(公告)号：KR101383546B1
公开(公告)日：2014-04-10
申请号：KR1020070104219
申请日：2007-10-16
Applicant: 한국해양과학기술원
Abstract: 본 발명은 심해 해저에서 분리된 리파제 및 에스터라제 효소 및 이의 제조방법에 관한 것으로서, 서남태평양 해역(남위 3°89′, 동경 152°49′) 깊이 1,400 미터에 이르는 심해 퇴적물, 북극 다산기지 주변 연안 퇴적물, 강화도 갯벌 퇴적물로부터 구축된 메타게놈 라이브러리를 대상으로 분리한 신규 리파제 및 에스터라제 효소, 이들을 암호화 하는 유전자 및 리파제 및 에스터라제 효소 생산방법을 제공한다.
리파제, 에스터라제-
公开(公告)号：KR101736485B1
公开(公告)日：2017-05-16
申请号：KR1020130033345
申请日：2013-03-28
Applicant: 한국해양과학기술원
CPC classification number: C12P3/00 , C12N9/0067 , C12Y112/98001
Abstract: 본발명은포름산염으로부터수소생산능이증가된써모코코스온누리우스엔에이원돌연변이체및 이를이용한수소생산방법에관한것이다. 본발명에따라돌연변이화된NA1는야생형보다포름산염을포함하는배지에서수소생산능력이증가되었고, 생장속도도야생형에비해증가하였다. 본발명에따른균주를이용하면포름산으로부터고효율로수소를생산할수 있다.
Abstract translation: 本发明涉及由甲酸盐产生的具有提高的产氢能力的Thermococcus onneriensis突变体以及使用该突变体产生氢的方法。 根据本发明，与野生型相比，突变型NA1增加了含有甲酸盐的培养基中的氢生产能力和生长速率。 使用根据本发明的菌株，可以从甲酸高效生产氢。
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公开(公告)号：KR1020140118079A
公开(公告)日：2014-10-08
申请号：KR1020130033345
申请日：2013-03-28
Applicant: 한국해양과학기술원
CPC classification number: C12P3/00 , C12N9/0067 , C12Y112/98001
Abstract: The present invention relates to a Thermococcus onnurineus NA1 mutant having improved hydrogen producibility from formate and a method for producing hydrogen using the same. According to the present, the mutated Thermococcus onnurineus NA1 has improved hydrogen producibility in a medium including formate rather than a wild type, and improved growth velocity compared to the wild type. If a strain according to the present invention is used, hydrogen can be produced from formic acid at high efficiency.
Abstract translation: 本发明涉及具有从甲酸盐提高的氢生产能力的嗜热热球菌NA1突变体和使用其产生氢的方法。 根据本发明，突变的Thermococcus onnurineus NA1在包括甲酸而不是野生型的培养基中具有改善的氢生产能力，并且与野生型相比改善了生长速度。 如果使用根据本发明的菌株，则可以高效地从甲酸生产氢。
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Patent Agency Ranking
公开(公告)号：KR1020140025686A
公开(公告)日：2014-03-05
申请号：KR1020120091634
申请日：2012-08-22
Applicant: 한국해양과학기술원
CPC classification number: C12N9/80 , C12P17/10 , C12Y305/01004
Abstract: The present invention relates to novel amidase, a gene encoding thereof, and a production method of caprolactam by using the amidase and the gene. The amidase of the present invention is capable of producing alpha-amino-epsilon-caprolactam from lysine. The alpha-amino-epsilon-caprolactam is a material for nylon and is internationally mass-produced. Therefore, the present invention provides a novel method of producing the alpha-amino-epsilon-caprolactam which is an environmentally friendly material for nylon.
Abstract translation: 本发明涉及新型酰胺酶，其编码基因，以及使用酰胺酶和基因的己内酰胺的制造方法。 本发明的酰胺酶能够从赖氨酸产生α-氨基 - 己内酰胺。 α-氨基-ε-己内酰胺是用于尼龙的材料，并且是国际上批量生产的。 因此，本发明提供了一种生产用于尼龙的环保材料的α-氨基 - ε-己内酰胺的新方法。
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27.发明公开개미산을 이용해 수소를 생산해 낼 수 있는 Thermococcus spp. 로부터 분리된 신규한 서열번호 7의 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 有权序列号7的新型氢化物，从热能SPP中纯化。 通过使用FORMATE，编码它们的基因，以及使用具有基因的微生物生产氢的方法
公开(公告)号：KR1020140022082A
公开(公告)日：2014-02-21
申请号：KR1020137034430
申请日：2009-09-07
Applicant: 한국해양과학기술원
CPC classification number: C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52
Abstract: The present invention relates to novel hydrogenases separated from new hyperthermophilic strains belonging to Thermococcus genus, genes encoding the same, and a method for producing hydrogen using the same. According to a method for producing hydrogen of the present invention, a large amount of hydrogen can be produced by culturing the strains in a specific culture condition and therefore, it is more economical and effective compared to the existing method for producing hydrogen and hydrogen can be produced at a high temperature.
Abstract translation: 本发明涉及从属于Thermococcus属的新的超嗜热菌株分离出的新型氢化酶，以及使用该氢过氧化氢的方法。 根据本发明的氢的制造方法，可以通过在特定培养条件下培养菌株来生产大量氢，因此与现有的氢气制备方法相比，其更经济有效，氢可以是 在高温下生产。
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28.发明公开개미산을 이용해 수소를 생산해 낼 수 있는 Thermococcus spp. 로부터 분리된 신규한 서열번호 2의 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 无效序列号2的新型氢化物，其从热能SPP中纯化。 通过使用FORMATE，编码它们的基因，以及使用具有基因的微生物生产氢的方法
公开(公告)号：KR1020140022081A
公开(公告)日：2014-02-21
申请号：KR1020137034429
申请日：2009-09-07
Applicant: 한국해양과학기술원
CPC classification number: C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52
Abstract: The present invention relates to novel hydrogenases separated from new hyperthermophilic strains belonging to Thermococcus genus, genes encoding the same, and a method for producing hydrogen using the same. According to a method for producing hydrogen of the present invention, a large amount of hydrogen can be produced by culturing the strains in a specific culture condition and therefore, it is more economical and effective compared to the existing method for producing hydrogen and hydrogen can be produced at a high temperature.
Abstract translation: 本发明涉及从属于Thermococcus属的新的超嗜热菌株分离出的新型氢化酶，以及使用该氢过氧化氢的方法。 根据本发明的氢的制造方法，可以通过在特定培养条件下培养菌株来生产大量氢，因此与现有的氢气制备方法相比，其更经济有效，氢可以是 在高温下生产。
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公开(公告)号：KR1020110126012A
公开(公告)日：2011-11-22
申请号：KR1020100045692
申请日：2010-05-14
Applicant: 한국해양과학기술원
Abstract: PURPOSE: A DNA polymerase F7 and genes thereof are provided to enhance elongation and processivity and to be used in PCR. CONSTITUTION: A DNA polymerase F7 contains an amino acid sequence of sequence number 2. A DNA polymerase F7 gene encoding an amino acid sequence of sequence number 2 contains a base of sequence number 1. A recombinant vector contains the polymerase F7 gene of sequence number 1. A host cell is transformed with the recombinant vector containing the DNA polymerase F7 gene.
Abstract translation: 目的：提供DNA聚合酶F7及其基因以增加伸长和持续性，并用于PCR。 构成：DNA聚合酶F7含有序列号2的氨基酸序列。编码序列号2的氨基酸序列的DNA聚合酶F7基因含有序列号1的碱基。重组载体含有序列号1的聚合酶F7基因 用含有DNA聚合酶F7基因的重组载体转化宿主细胞。
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30.发明公开일산화탄소를 이용해 수소를 생산해 낼 수 있는 Ｔｈｅｒｍｏｃｃｕｓ ｓｐｐ．로부터 분리된 신규한 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 无效来自热能SPP的新型氢化物2。 通过使用碳黑单体，编码它们的基因，以及使用具有基因的微生物生产氢的方法
公开(公告)号：KR1020110094092A
公开(公告)日：2011-08-19
申请号：KR1020117014737
申请日：2009-09-07
Applicant: 한국해양과학기술원
CPC classification number: C12N9/0006 , C12N9/0067 , C12P3/00 , C12R1/01 , Y02P20/132 , Y02P20/52
Abstract: PURPOSE: A novel hydrogenase isolated from Thermococcus spp. and a method for producing hydrogen using the same are provided to produce hydrogen without high temperature and pressure. CONSTITUTION: A hydrogenase contains an amino acid sequence of sequence number 5. A gene encoding the hydrogenase contains a base sequence of sequence number 16. A method for producing hydrogen from Thermococcus spp. comprises: a step of preparing a medium; a step of culturing Thermococcus spp. in the medium; and a step of isolating hydrogen. The medium contains carbon monoxide. The Thermococcus spp. is Thermococcus onnurineus NA1(deposit number: KCTC 10859BP).
Abstract translation: 目的：从Thermococcus spp。分离出的新型氢化酶。 并且提供使用其制造氢的方法以在没有高温和高压的情况下产生氢。 构成：氢化酶含有序列号5的氨基酸序列。编码氢化酶的基因含有序列号16的碱基序列。从Thermococcus spp。生产氢的方法。 包括：制备介质的步骤; 培养热球菌属的一个步骤。 在媒体中 和分离氢的步骤。 介质含有一氧化碳。 热球菌属 是Thermococcus onnurineus NA1（保藏号：KCTC 10859BP）。
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