Abstract:
The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. At least one adhesive layer adhered to the base or the coversheet in the growth compartment. A cold water-soluble gelling agent and a dry oxygen-scavenging reagent are adhered to the at least one adhesive layer. The oxygen-scavenging reagent consists essentially of particles having a diameter of less than 106 microns.
Abstract:
A device for culturing anaerobic microorganisms is provided. The device comprises a body comprising a waterproof base, a waterproof coversheet attached to the base, and a growth compartment disposed between the base and the coversheet. The growth compartment has a perimeter and an opening that provides liquid access to the growth compartment. A portion of the perimeter is defined by a waterproof seal. The portion includes >50% of the perimeter. A dry cold water-soluble gelling agent is adhered to the base in the growth compartment. A dry first oxygen-scavenging reagent is disposed in the growth compartment.
Abstract:
Methods of detecting microorganisms in a fluid sample are provided. The method includes providing a nonwoven article, providing a fluid sample suspected of containing at least one microorganism strain or target cellular analyte, and contacting the fluid sample with the nonwoven article such that at least a portion of the at least one microorganism strain or target cellular analyte is bound to the nonwoven article. The nonwoven article includes a fibrous porous matrix and a concentration agent particles enmeshed in the fibrous porous matrix. The method further includes placing the microorganism strain- or target cellular analyte-bound nonwoven article in contact with at least one detection reagent and detecting the presence of the bound microorganism strain or bound target cellular analyte. Devices and kits containing the devices, for contacting a fluid sample with a nonwoven article, are also provided. The device includes a sample container, a filter holder, a nonwoven article, and an adaptor configured to interface the filter holder with a receptacle.
Abstract:
Methods for using a hollow, rotating stencil roll to deposit flowable dry powder particles onto a moving substrate and to attach the particles to the substrate.