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    MICROBIAL DETECTION DEVICES INCLUDING ADHESIVE MASKED NUTRIENTS AND METHODS OF USING THE SAME
    1.
    发明申请
    MICROBIAL DETECTION DEVICES INCLUDING ADHESIVE MASKED NUTRIENTS AND METHODS OF USING THE SAME 审中-公开

    公开(公告)号：WO2018125805A1

    公开(公告)日：2018-07-05

    申请号：PCT/US2017/068213

    申请日：2017-12-22

    Applicant: 3M INNOVATIVE PROPERTIES COMPANY

    Inventor： YOUNG, Alexi J. ,  BRUTINEL, Evan D.

    IPC: C12M1/12 ,  C12Q1/04

    Abstract: Devices for microbial detection of microorganisms are provided including a body member including a substrate having a first major surface and a second major surface. The device further includes a substantially dry, first microbial growth nutrient composition disposed on a portion of the first major surface, a first adhesive composition adhered to the first microbial growth nutrient composition, and a cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition. The device also includes a cover sheet attached to the body member, where the cover sheet includes a first major surface facing the body member. Devices including a water-proof pouch are also provided. Methods for detecting and enumerating at least one microorganism in a sample are additionally provided, using the devices.

    ASSAY DEVICES AND METHOD OF DETECTING A TARGET ANALYTE
    3.
    发明申请
    ASSAY DEVICES AND METHOD OF DETECTING A TARGET ANALYTE 审中-公开
    测定装置和检测目标分析仪的方法

    公开(公告)号：WO2015191419A2

    公开(公告)日：2015-12-17

    申请号：PCT/US2015/034624

    申请日：2015-06-08

    Applicant: 3M INNOVATIVE PROPERTIES COMPANY

    Inventor： ROSCOE, Stephen B. ,  KANG, Myungchan ,  BRUTINEL, Evan D. ,  MILLER, Jesse D. ,  CLINE, Renee K. ,  DAI, Minghua ,  LIU, Hsi-Chou

    CPC classification number: B01L3/5029 ,  B01L3/502 ,  B01L2200/16 ,  B01L2300/0825 ,  B01L2400/0406 ,  B01L2400/0478 ,  G01N33/5304

    Abstract: Assay devices are provided including a receptacle having a sample entry port; a plunger disposed within the receptacle; at least one reagent; a membrane attached to a first surface of the plunger; a wick providing capillary force; and a detection zone. The membrane includes a target conjugate zone in which affinity components are disposed. Capture compounds are immobilized in a detection zone, which is located between the target conjugate zone and the wick. A method of detecting a target analyte is also provided, including providing the assay device; providing a sample suspected to contain a target analyte; adding the sample onto the device; allowing the sample to travel along the membrane until the sample reaches the detection zone; immobilizing the target analyte through reaction of the target analyte with the capture compounds; reacting the immobilized target analyte with a reagent to generate a detectable signal; and detecting the generated signal.

    Abstract translation: 提供测定装置，包括具有样品入口的容器; 设置在所述容器内的柱塞; 至少一种试剂; 膜附接到柱塞的第一表面; 提供毛细管力的灯芯; 和检测区。 膜包括其中配置亲和成分的靶共轭区。 捕获化合物被固定在位于靶共轭区和芯之间的检测区中。 还提供了检测目标分析物的方法，包括提供测定装置; 提供怀疑含有目标分析物的样品; 将样品添加到设备上; 允许样品沿着膜移动直到样品到达检测区域; 通过靶分析物与捕获化合物的反应固定目标分析物; 使固定化的目标分析物与试剂反应以产生可检测的信号; 并检测所生成的信号。

    THIN FILM CULTURE DEVICE WITH CARBON DIOXIDE GENERANT
    5.
    发明申请
    THIN FILM CULTURE DEVICE WITH CARBON DIOXIDE GENERANT 审中-公开
    具有二氧化碳生成物的薄膜文化装置

    公开(公告)号：WO2016176178A1

    公开(公告)日：2016-11-03

    申请号：PCT/US2016/029309

    申请日：2016-04-26

    Applicant: 3M INNOVATIVE PROPERTIES COMPANY

    Inventor： BJORK, Jason W. ,  BRUTINEL, Evan D. ,  STANENAS, Adam J.

    IPC: C12M1/12 ,  C12M1/34

    CPC classification number: C12M25/06 ,  C12M23/04 ,  C12M23/20 ,  C12M23/34 ,  C12M41/34 ,  C12M41/36

    Abstract: The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. At least one adhesive layer is adhered to the base or the coversheet in the growth compartment. A cold water-soluble gelling agent and an effective amount of a dry carbon dioxide-generating reagent are adhered to the at least one adhesive layer. The carbon dioxide-generating reagent consists essentially of particles having a diameter of less than 106 microns.

    Abstract translation: 本公开提供了一种用于列举微生物菌落的培养装置。 该装置可以包括底座，覆盖片和设置在它们之间的无孔隔离构件。 间隔构件包括限定生长室的孔。 至少一个粘合剂层粘附到生长室中的基底或覆盖片上。 冷水溶性胶凝剂和有效量的干二氧化碳生成剂粘附到至少一个粘合剂层。 产生二氧化碳的试剂基本上由直径小于106微米的颗粒组成。

    CULTURE DEVICE FOR LACTIC ACID BACTERIA
    6.
    发明申请
    CULTURE DEVICE FOR LACTIC ACID BACTERIA 审中-公开
    用于酸乳杆菌的培养装置

    公开(公告)号：WO2016176173A1

    公开(公告)日：2016-11-03

    申请号：PCT/US2016/029303

    申请日：2016-04-26

    Applicant: 3M INNOVATIVE PROPERTIES COMPANY

    Inventor： BJORK, Jason W. ,  BRUTINEL, Evan D. ,  STANENAS, Adam J.

    IPC: C12M1/12 ,  C12M1/34 ,  C12Q1/06 ,  C12M1/00 ,  C12Q1/04

    CPC classification number: C12M25/06 ,  C12M23/04 ,  C12M23/20 ,  C12M41/34 ,  C12M41/36 ,  C12Q1/04

    Abstract: The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. Disposed in the growth compartment are a cold water-soluble gelling agent, a dry oxygen-scavenging reagent, a dry buffer system, and an effective amount of a dry carbon dioxide-generating reagent. The buffer system is selected such that, when the growth compartment is hydrated with a predetermined volume of deionized water, an aqueous mixture with a pH less than or equal to 6.35 is formed.

    Abstract translation: 本公开提供了一种用于列举微生物菌落的培养装置。 该装置可以包括底座，覆盖片和设置在它们之间的无孔隔离构件。 间隔构件包括限定生长室的孔。 在生长室中配置有冷水溶性胶凝剂，干燥氧清除剂，干燥缓冲系统和有效量的产生干燥二氧化碳的试剂。 选择缓冲系统，使得当生长室与预定体积的去离子水水合时，形成pH小于或等于6.35的含水混合物。

    DEVICE FOR GROWING MICROORGANISMS
    7.
    发明申请
    DEVICE FOR GROWING MICROORGANISMS 审中-公开

    公开(公告)号：WO2020260984A1

    公开(公告)日：2020-12-30

    申请号：PCT/IB2020/055126

    申请日：2020-05-29

    Applicant: 3M INNOVATIVE PROPERTIES COMPANY

    Inventor： YOUNG, Alexi J. ,  HALVERSON, Kurt J. ,  SWANSON, Steven P. ,  BRUTINEL, Evan D. ,  NELSON, Caleb T.

    IPC: C12M1/12 ,  C12M1/00 ,  C12Q1/04

    Abstract: A device for growing microorganisms. The device includes a body member comprising a self-supporting, water-proof substrate having upper and lower surfaces; a hydrophobic spacer element adhered to the upper surface of the substrate forming side walls to retain a predetermined amount of liquid in contact with the substrate, wherein the hydrophobic spacer element has a hole therein; a fluid control film in the hole of the hydrophobic spacer element; a cover sheet having an inner-facing surface and an outer-facing surface, the cover sheet adhered to at least a portion of the body member; and a substantially dry, first microbial growth nutrient composition disposed on a portion of the inner surface of the cover sheet; a first adhesive composition adhered to the first microbial growth nutrient composition; and a cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition.

    SELECTIVE THIN-FILM CULTURE DEVICE FOR ENUMERATING MICROORGANISMS
    8.
    发明申请
    SELECTIVE THIN-FILM CULTURE DEVICE FOR ENUMERATING MICROORGANISMS 审中-公开

    公开(公告)号：WO2019213332A1

    公开(公告)日：2019-11-07

    申请号：PCT/US2019/030297

    申请日：2019-05-02

    Applicant: 3M INNOVATIVE PROPERTIES COMPANY

    Inventor： YOUNG, Alexi J. ,  BRUTINEL, Evan D. ,  MACH, Patrick A. ,  STANENAS, Adam J.

    IPC: C12M1/00 ,  C12M1/12 ,  C12M1/34

    Abstract: A selective microbial detection device and methods of use are provided. The device includes a water-proof pouch that includes a first wall portion, a second wall portion, and a porous membrane filter disposed in the pouch between the first and second wall portions. The filter membrane divides the pouch into first and second compartments. The microbial detection device also includes an effective amount of a dry nutrient disposed in the first compartment, which contains sodium lauryl sulfate in an amount of 1.75 milligrams or greater per twelve square inches of the first wall portion. A dry, cold water-soluble gelling agent is adhered to the pouch in the first compartment and an absorbent pad is disposed in the second compartment. A sealable sample port provides access to deposit a liquid into the first compartment.

    DEVICES AND METHODS FOR SAMPLE PARTITIONING AND ANALYSIS
    10.
    发明申请
    DEVICES AND METHODS FOR SAMPLE PARTITIONING AND ANALYSIS 审中-公开
    用于样本分析和分析的设备和方法

    公开(公告)号：WO2016028834A1

    公开(公告)日：2016-02-25

    申请号：PCT/US2015/045786

    申请日：2015-08-19

    Applicant: 3M INNOVATIVE PROPERTIES COMPANY

    Inventor： BRUTINEL, Evan D. ,  HALVERSON, Kurt J.

    IPC: C12M1/34 ,  C12Q1/04 ,  G01N33/52 ,  C12M1/32 ,  C12M1/00 ,  C12M1/12

    CPC classification number: C12Q1/06 ,  C12M23/34 ,  C12M23/38 ,  C12M25/06 ,  C12M37/04 ,  C12M41/36 ,  C12Q1/04

    Abstract: The present disclosure provides a device that includes a base comprising a substrate having a first major surface, a pressure sensitive adhesive adhered to at least a portion of the first major surface, a polymeric cover film coupled to the substrate via the adhesive, a plurality of isolated closed compartments disposed between the substrate and the cover film, and an aqueous liquid disposed in two or more of the closed compartments. The cover film is a composite film comprising ethylene vinyl acetate copolymer, a linear copolymer of ethylene and a higher alkene, and a tackifier. Each compartment of the plurality is defined by a seal that prevents liquid communication with at least one other compartment of the plurality. Methods of using the device for partitioning a sample, for analyzing a sample, and for culturing a microorganism are also provided.

    Abstract translation: 本公开提供了一种装置，其包括基底，该基底包括具有第一主表面的基底，粘附到第一主表面的至少一部分的压敏粘合剂，经由粘合剂连接到基底的聚合物覆盖膜，多个 设置在基板和覆盖膜之间的分离的封闭隔室，以及设置在两个或更多个封闭隔室中的水性液体。 覆盖膜是包含乙烯乙酸乙烯酯共聚物，乙烯和高级烯烃的线性共聚物和增粘剂的复合膜。 多个的每个隔室由密封件限定，该密封件防止与多个的至少一个其他隔室的液体连通。 还提供了使用该装置分离样品，分析样品和培养微生物的方法。

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