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公开(公告)号：WO2019213332A1
公开(公告)日：2019-11-07
申请号：PCT/US2019/030297
申请日：2019-05-02
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： YOUNG, Alexi J. , BRUTINEL, Evan D. , MACH, Patrick A. , STANENAS, Adam J.
Abstract: A selective microbial detection device and methods of use are provided. The device includes a water-proof pouch that includes a first wall portion, a second wall portion, and a porous membrane filter disposed in the pouch between the first and second wall portions. The filter membrane divides the pouch into first and second compartments. The microbial detection device also includes an effective amount of a dry nutrient disposed in the first compartment, which contains sodium lauryl sulfate in an amount of 1.75 milligrams or greater per twelve square inches of the first wall portion. A dry, cold water-soluble gelling agent is adhered to the pouch in the first compartment and an absorbent pad is disposed in the second compartment. A sealable sample port provides access to deposit a liquid into the first compartment.
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2.发明申请SYSTEM AND METHOD FOR LIQUID CULTURE OF ANAEROBIC OR MICROAEROPHILIC MICROORGANISMS 审中-公开无机或微生物微生物液体培养系统与方法
公开(公告)号：WO2015175611A1
公开(公告)日：2015-11-19
申请号：PCT/US2015/030491
申请日：2015-05-13
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： BJORK, Jason W. , STANENAS, Adam J. , XIA, Wensheng
Abstract: A culture system for culturing a microaerophilic or an anaerobic microorganism is provided. The culture system can include effective amounts of i) an enzyme of an oxidoreductase family and ii) a substrate for said enzyme, a container, and a predetermined volume of aqueous medium that supports growth of said anaerobic or microaerophilic microorganism. The enzyme can be selected from a group consisting of ascorbic acid oxidase and laccase. The effective amounts are effective to deplete dissolved oxygen in the predetermined volume to a concentration that facilitates growth of a microaerophilic microorganism or an obligately-anaerobic microorganism. A method of using the system is also provided.
Abstract translation: 提供了用于培养微嗜酸或厌氧微生物的培养系统。 培养系统可以包括有效量的i）氧化还原酶家族的酶，和ii）所述酶，容器和支持所述厌氧微生物微生物生长的预定体积的水性介质的底物。 酶可以选自抗坏血酸氧化酶和漆酶组。 有效量有效地将预定体积中的溶解氧消耗至促进微生物微生物或专一性厌氧微生物生长的浓度。 还提供了一种使用该系统的方法。
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3.发明申请
公开(公告)号：WO2017058737A1
公开(公告)日：2017-04-06
申请号：PCT/US2016/053863
申请日：2016-09-27
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： BRUTINEL, Evan D. , BJORK, Jason W. , STANENAS, Adam J.
Abstract: In one aspect, the present disclosure provides a device. The device includes a body comprising a waterproof base, a waterproof coversheet attached to the base, and a channel disposed between the base and the coversheet. The channel has a perimeter and an opening that provides liquid access to the channel. A portion of the perimeter is defined by a waterproof seal. A dry first oxygen-scavenging reagent and an indicator reagent for detecting sulfate reduction by a sulfate-reducing bacterium are disposed in the device between the base and the coversheet. The waterproof base comprises a plurality of open microcompartment structures facing the coversheet.
Abstract translation: 一方面，本公开提供了一种装置。 该装置包括主体，该主体包括防水基座，附接到基座的防水覆盖片以及设置在基座和盖板之间的通道。 通道具有周边和开口，可以提供液体进入通道。 周边的一部分由防水密封限定。 干燥的第一氧气清除试剂和用于通过硫酸盐还原细菌检测硫酸盐还原的指示剂被设置在基底和覆盖片之间的装置中。 防水底座包括面向盖板的多个开放微型隔室结构。
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公开(公告)号：WO2016176176A1
公开(公告)日：2016-11-03
申请号：PCT/US2016/029306
申请日：2016-04-26
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： BJORK, Jason W. , BRUTINEL, Evan D. , STANENAS, Adam J.
CPC classification number: C12Q1/06 , C12M23/04 , C12M23/20 , C12M23/34 , C12M25/06 , C12M41/34 , C12M41/36
Abstract: The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. At least one adhesive layer adhered to the base or the coversheet in the growth compartment. A cold water-soluble gelling agent and a dry oxygen-scavenging reagent are adhered to the at least one adhesive layer. The oxygen-scavenging reagent consists essentially of particles having a diameter of less than 106 microns.
Abstract translation: 本公开提供了一种用于列举微生物菌落的培养装置。 该装置可以包括底座，覆盖片和设置在它们之间的无孔隔离构件。 间隔构件包括限定生长室的孔。 至少一个粘合剂层粘附到生长室中的基底或覆盖片上。 冷水溶胶剂和干氧清除试剂粘附到至少一个粘合剂层。 氧气清除剂基本上由直径小于106微米的颗粒组成。
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公开(公告)号：WO2016176178A1
公开(公告)日：2016-11-03
申请号：PCT/US2016/029309
申请日：2016-04-26
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： BJORK, Jason W. , BRUTINEL, Evan D. , STANENAS, Adam J.
Abstract: The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. At least one adhesive layer is adhered to the base or the coversheet in the growth compartment. A cold water-soluble gelling agent and an effective amount of a dry carbon dioxide-generating reagent are adhered to the at least one adhesive layer. The carbon dioxide-generating reagent consists essentially of particles having a diameter of less than 106 microns.
Abstract translation: 本公开提供了一种用于列举微生物菌落的培养装置。 该装置可以包括底座，覆盖片和设置在它们之间的无孔隔离构件。 间隔构件包括限定生长室的孔。 至少一个粘合剂层粘附到生长室中的基底或覆盖片上。 冷水溶性胶凝剂和有效量的干二氧化碳生成剂粘附到至少一个粘合剂层。 产生二氧化碳的试剂基本上由直径小于106微米的颗粒组成。
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Patent Agency Ranking
公开(公告)号：WO2016176173A1
公开(公告)日：2016-11-03
申请号：PCT/US2016/029303
申请日：2016-04-26
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： BJORK, Jason W. , BRUTINEL, Evan D. , STANENAS, Adam J.
Abstract: The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. Disposed in the growth compartment are a cold water-soluble gelling agent, a dry oxygen-scavenging reagent, a dry buffer system, and an effective amount of a dry carbon dioxide-generating reagent. The buffer system is selected such that, when the growth compartment is hydrated with a predetermined volume of deionized water, an aqueous mixture with a pH less than or equal to 6.35 is formed.
Abstract translation: 本公开提供了一种用于列举微生物菌落的培养装置。 该装置可以包括底座，覆盖片和设置在它们之间的无孔隔离构件。 间隔构件包括限定生长室的孔。 在生长室中配置有冷水溶性胶凝剂，干燥氧清除剂，干燥缓冲系统和有效量的产生干燥二氧化碳的试剂。 选择缓冲系统，使得当生长室与预定体积的去离子水水合时，形成pH小于或等于6.35的含水混合物。
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公开(公告)号：WO2021161151A1
公开(公告)日：2021-08-19
申请号：PCT/IB2021/051010
申请日：2021-02-08
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： PERCY, Neil , XIA, Wensheng , STANENAS, Adam J.
Abstract: A culture device comprising an oxygen sensitive luminophore, typically an oxygen sensitive phosphor, such as a porphyrin, and methods of use for culturing and enumerating microorganisms.
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8.发明申请SELF-CONTAINED ANAEROBIC CULTURE DEVICE FOR SULFATE-REDUCING MICROORGANISMS 审中-公开用于硫酸还原微生物的自含式厌氧培养装置
公开(公告)号：WO2016028839A1
公开(公告)日：2016-02-25
申请号：PCT/US2015/045795
申请日：2015-08-19
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： BRUTINEL, Evan D. , BJORK, Jason W., , STANENAS, Adam J.
CPC classification number: C12M33/00 , C12M23/04 , C12M23/20 , C12M25/06 , C12M37/04 , C12M41/36 , C12Q1/04
Abstract: The present disclosure provides a culture device for enumerating colonies of sulfate-reducing microorganisms. The device includes a body having a waterproof base, a waterproof coversheet attached to the base, and a growth compartment disposed therebetween. The growth compartment has a perimeter and an opening. A portion of the perimeter is defined by a waterproof seal. The portion can include >50% of the perimeter. Disposed in the growth compartment are a dry cold water-soluble gelling agent, a dry culture medium selected to facilitate growth of a sulfate-reducing bacterium or indicator reagent for detecting hydrogen sulfide production by a sulfate-reducing bacterium, and a dry first oxygen-scavenging reagent.
Abstract translation: 本公开提供了一种用于计数硫酸盐还原微生物菌落的培养装置。 该装置包括具有防水基座的主体，附接到基座的防水覆盖片，以及设置在其间的生长室。 生长室具有周长和开口。 周边的一部分由防水密封限定。 该部分可以包括> 50％的周长。 在生长室中设置干燥的冷水溶性胶凝剂，选择用于促进硫酸盐还原细菌的生长的干燥培养基或用于检测硫酸盐还原细菌产生硫化氢的指示剂，以及干燥的第一氧 - 清除剂。
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公开(公告)号：WO2013101590A1
公开(公告)日：2013-07-04
申请号：PCT/US2012/070580
申请日：2012-12-19
Applicant: 3M INNOVATIVE PROPERTIES COMPANY , BINSFELD, Christine A. , MACH, Patrick A. , REIF-WENNER, Mara S. , STANENAS, Adam J.
Inventor： BINSFELD, Christine A. , MACH, Patrick A. , REIF-WENNER, Mara S. , STANENAS, Adam J.
CPC classification number: C12Q1/10 , C12Q1/045 , G01N2333/255 , Y02A50/451
Abstract: A composition is provided for detecting a Salmonella microorganism in sample. The composition comprises at least one first selective agent that inhibits the growth of Gram-positive microorganisms, a first differential indicator system comprising at least one first differential indicator compound that is converted to a first detectable product by a Salmonella microorganism, and a second differential indicator system comprising a second differential indicator compound that is converted by urease enzyme activity to a second detectable product. Optionally, the composition may comprise a third differential indicator system comprising a third differential indicator compound that is converted by a β -galactosidase enzyme activity to a third detectable product. Methods of using the composition to detect a Salmonella microorganism are also provided.
Abstract translation: 提供了用于检测样品中的沙门氏菌微生物的组合物。 所述组合物包含至少一种抑制革兰氏阳性微生物生长的第一选择剂，第一差异指示剂系统，其包含至少一种由沙门氏菌微生物转化为第一可检测产物的第一差异指示剂化合物和第二差异指示剂 系统，其包含通过脲酶酶活性转化为第二可检测产物的第二差异指示剂化合物。 任选地，组合物可以包含第三差异指示剂系统，其包含由β-半乳糖苷酶活性转化为第三可检测产物的第三差异指示剂化合物。 还提供了使用组合物检测沙门氏菌微生物的方法。
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公开(公告)号：WO2013101530A1
公开(公告)日：2013-07-04
申请号：PCT/US2012/070223
申请日：2012-12-18
Applicant: 3M INNOVATIVE PROPERTIES COMPANY , BINSFELD, Christine A. , MACH, Patrick A. , REIF-WENNER, Mara S. , STANENAS, Adam J.
Inventor： BINSFELD, Christine A. , MACH, Patrick A. , REIF-WENNER, Mara S. , STANENAS, Adam J.
CPC classification number: C12Q1/10 , C12Q1/04 , C12Q1/34 , C12Q2304/00 , C12Q2334/00 , C12Y302/01023 , Y02A50/451
Abstract: A method of detecting a Salmonella microorganism is provided. The method includes the use of a selective growth medium, a first indicator system that is converted to a first detectable product by a Salmonella microorganism, and a second indicator system that is converted to a second detectable product by β-galactosidase enzyme activity. The method further comprises inoculating the growth medium and incubating the inoculated growth medium at a temperature higher than 40 degrees C.
Abstract translation: 提供了一种检测沙门氏菌微生物的方法。 该方法包括使用选择性生长培养基，通过沙门氏菌微生物转化为第一可检测产物的第一指示剂系统和通过β-半乳糖苷酶活性转化为第二可检测产物的第二指示剂系统。 该方法还包括接种生长培养基并在高于40摄氏度的温度下孵育接种的生长培养基。
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