公开(公告)号：AU1141601A

公开(公告)日：2001-05-14

申请号：AU1141601

申请日：2000-10-20

Applicant: BASF AG

Inventor： KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  MATTES RALF

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/16 ,  C12N9/18 ,  C12N15/55 ,  C12P17/04 ,  C12P41/00 ,  C12R1/19

Abstract: The present invention relates to proteins which have an enzymatic activity for hydrolyzing L-pantolactone. The invention further relates to nucleic acids which code for these proteins, to nucleic acid constructs, vectors, genetically modified microorganisms and to a process for preparing D-pantolactone.

公开(公告)号：AU2004215238B2

公开(公告)日：2009-08-27

申请号：AU2004215238

申请日：2004-02-24

Applicant: BASF AG

Inventor： ZELINSKI THOMAS ,  FRIEDRICH THOMAS ,  KESSELER MARIA ,  HAUER BERNHARD

IPC: C12N9/78 ,  C12N15/55 ,  C12N15/82 ,  C12P7/40

Abstract: The present Invention relates to new nitrilases with modified Substrate acceptance, the method to obtain them and the use of the aforesaid nitrilases. Said nitrilases are encoded by a polypeptide sequence, which at a Position corresponding to Position 296 in wild-type Alcaligenes faecalis comprises an amino acid which is not tyrosine. The Invention further relates to nucleic acid sequences and amino acid sequences encoding Said nitrilases, expression constructs comprising Said nucleic acid sequences, vectors comprising the nucleic acid sequences or the exPression constructs, to organisms, preferably microorganisms, comprising the nucleic acid sequences, expression constructs or vectors. The Invention additionally relates to a method for preparing carboxylic acids, preferably substituted chiral carboxylic acids from racemic nitriles.

公开(公告)号：AT368746T

公开(公告)日：2007-08-15

申请号：AT03780076

申请日：2003-11-27

Applicant: BASF AG

Inventor： KESSELER MARIA ,  ZELINSKI THOMAS ,  HAUER BERNHARD

IPC: C12N15/63 ,  C12N15/70

公开(公告)号：AU2003296731A1

公开(公告)日：2004-08-10

申请号：AU2003296731

申请日：2003-12-24

Applicant: BASF AG

Inventor： ZELINSKI THOMAS ,  KESSELER MARIA ,  HAUER BERNHARD

IPC: A01N1/02 ,  C12N1/04 ,  C12N9/78 ,  C12N9/88 ,  C12N9/96

Abstract: The invention relates to a method for preserving and/or storing microorganisms which exhibit at least one nitrile hydratase or nitrilase enzyme activity, with the preservation and/or storage being effected in an aqueous medium which comprises at least one aldehyde, with the total aldehyde concentration being in a range from 0.1 to 100 mM/l.

公开(公告)号：AU2003288189A1

公开(公告)日：2004-06-23

申请号：AU2003288189

申请日：2003-11-27

Applicant: BASF AG

Inventor： KESSELER MARIA ,  ZELINSKI THOMAS ,  HAUER BERNHARD

IPC: C12N15/63 ,  C12N15/70

公开(公告)号：PL355487A1

公开(公告)日：2004-05-04

申请号：PL35548700

申请日：2000-10-20

Applicant: BASF AG

Inventor： KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  MATTES RALF

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/16 ,  C12N9/18 ,  C12N15/55 ,  C12P17/04 ,  C12P41/00 ,  C12R1/19

Abstract: The present invention relates to proteins which have an enzymatic activity for hydrolyzing L-pantolactone. The invention further relates to nucleic acids which code for these proteins, to nucleic acid constructs, vectors, genetically modified microorganisms and to a process for preparing D-pantolactone.

公开(公告)号：AU2003232256A1

公开(公告)日：2003-11-17

申请号：AU2003232256

申请日：2003-05-05

Applicant: BASF AG

Inventor： FRIEDRICH THOMAS ,  KESSELER MARIA ,  HAUER BERNHARD

IPC: C12N9/88 ,  C12N15/60 ,  C12P7/42 ,  C12P13/00 ,  C12P41/00

公开(公告)号：CZ20021449A3

公开(公告)日：2002-10-16

申请号：CZ20021449

申请日：2000-10-20

Applicant: BASF AG

Inventor： KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  MATTES RALF

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/16 ,  C12N9/18 ,  C12N15/55 ,  C12P17/04 ,  C12P41/00 ,  C12R1/19

Abstract: The present invention relates to proteins which have an enzymatic activity for hydrolyzing L-pantolactone. The invention further relates to nucleic acids which code for these proteins, to nucleic acid constructs, vectors, genetically modified microorganisms and to a process for preparing D-pantolactone.

公开(公告)号：CA2389064A1

公开(公告)日：2001-05-10

申请号：CA2389064

申请日：2000-10-20

Applicant: BASF AG

Inventor： KESSELER MARIA ,  MATTES RALF ,  FRIEDRICH THOMAS ,  HAUER BERNHARD

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/16 ,  C12N9/18 ,  C12N15/55 ,  C12P17/04 ,  C12P41/00 ,  C12R1/19

Abstract: The invention relates to proteins which have an enzymatic activity for hydrolysing L-pantolactone. The invention also relates to nucleic acids that code for these proteins, to nucleic acid constructs, to vectors, to genetically modified micro-organisms and to a method for producing D- pantolactone.

公开(公告)号：CA2512154C

公开(公告)日：2013-09-17

申请号：CA2512154

申请日：2003-12-24

Applicant: BASF AG

Inventor： ZELINSKI THOMAS ,  KESSELER MARIA ,  HAUER BERNHARD

IPC: C12N1/04 ,  A01N1/02 ,  C12N9/78 ,  C12N9/88 ,  C12N9/96

Abstract: The invention relates to methods for preserving and/or storing micro-organisms having at least a nitrile hydratase enzyme activity or a nitrilase enzyme activity. According to said method, the preservation and/or storage takes place in an aqueous medium containing at least one aldehyde, the total aldehyde concentration being between 0.1 and 100 mM/l.