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    METHOD FOR PRODUCING 5-NORBORNEN-2-CARBOXYLIC ACID FROM 5-NORBORNEN-2-CARBONITRILE USING AN ARYLACETONITRILASE
    1.
    发明申请
    METHOD FOR PRODUCING 5-NORBORNEN-2-CARBOXYLIC ACID FROM 5-NORBORNEN-2-CARBONITRILE USING AN ARYLACETONITRILASE 审中-公开
    用于生产从5降冰片烯-2-羧酸5-降冰片烯-2-腈使用甲arylacetonitrilase

    公开(公告)号：WO2007071578A3

    公开(公告)日：2007-11-08

    申请号：PCT/EP2006069511

    申请日：2006-12-11

    Applicant: BASF AG ,  KESSELER MARIA ,  HAUER BERNHARD

    Inventor： KESSELER MARIA ,  HAUER BERNHARD

    IPC: C12N15/55 ,  C07C51/08 ,  C12N1/21 ,  C12N9/78 ,  C12P7/40

    CPC classification number: C12P7/40 ,  C07C2601/10 ,  C12N9/78 ,  C12P41/006

    Abstract: The present invention relates to a method for producing 5-norbornen-2-carboxylic acid from 5-norbornen-2-endo-carbonitrile and/or 5-norbornen-2-exo-carbonitrile. The invention relates more specifically to a method for producing 5-norbornen-2-carboxylic acid at a high substrate concentration. Furthermore, the invention relates to a polypeptide suitable for the enzymatic reaction of 5-norbornen-2-carbonitrile to 5-norbornen-2-carboxylic acid, particularly also at a high substrate concentration, as well as to a nucleic acid coding for the polypeptide, a composition containing 5-norbornen-2-carbonitrile to 5-norbornen-2-endo-carboxylic acid and 5-norbornen-2-exo-carboxylic acid, as well as the use of the polypeptide.

    Abstract translation: 本发明涉及一种方法，用于从5-降冰片烯-2-内 - 腈和/或5-降冰片烯-2-外 - 腈制备的5-降冰片烯-2-羧酸。 特别地，本发明涉及一种方法，其中所述5-降冰片烯-2-羧酸可在高底物浓度而制备。 本发明还涉及适用于5-降冰片烯-2-甲腈的酶促转化为5-降冰片烯-2-羧酸，尤其是即使在高底物浓度的多肽，以及编码核酸的多肽，组合物，含有5-降冰片烯-2- 腈向5-降冰片烯-2-内 - 羧酸和5-降冰片烯-2-外 - 羧酸，和使用所述多肽的。

    SEQUENCES ENCODING S-HYDROXYNITRIL LYASES AND THEIR USE IN METHODS FOR THE PRODUCTION OF CYANOHYDRINS
    6.
    发明申请
    SEQUENCES ENCODING S-HYDROXYNITRIL LYASES AND THEIR USE IN METHODS FOR THE PRODUCTION OF CYANOHYDRINS 审中-公开
    编码S-羟肟酸盐的方法及其在生产氰尿酸的方法中的应用

    公开(公告)号：WO2004090124A3

    公开(公告)日：2004-11-25

    申请号：PCT/EP2004003844

    申请日：2004-04-13

    Applicant: BASF AG ,  FRIEDRICH THOMAS ,  KESSELER MARIA ,  HAUER BERNHARD

    Inventor： FRIEDRICH THOMAS ,  KESSELER MARIA ,  HAUER BERNHARD

    IPC: C12N9/88 ,  C12N15/60 ,  C12P13/00

    CPC classification number: C12P13/004 ,  C12N9/88

    Abstract: The present invention relates to new aminoacid sequences isolated from Adenia racemosa encoding S-hydroxynitrile lyases and their use in methods for the production of nitriles and/or carboxylic acids, preferably optically active nitriles and/or carboxylic acids. The invention further relates to nucleic acid encoding said S-hydroxynitrile lyases, recombinant expression constructs comprising said nucleic acid sequences, recombinant vectors comprising the nucleic acid sequences or the expression constructs, to recombinant organisms, preferably microorganisms or yeasts, comprising the nucleic acid sequences, expression constructs or vectors. The invention additionally relates to a method for the production of cyanohydrins and/or carboxylic acids, preferably optically active cyanohydrins and/or carboxylic acids from racemic aldehydes and cyanide.

    Abstract translation: 本发明涉及从编码S-羟基腈裂解酶的Adenia racemosa分离的新的氨基酸序列及其在制备腈和/或羧酸，优选光学活性腈和/或羧酸的方法中的用途。 本发明还涉及编码所述S-羟基腈裂解酶的核酸，包含所述核酸序列的重组表达构建体，包含核酸序列或表达构建体的重组载体，重组生物，优选微生物或酵母，其包含核酸序列， 表达构建体或载体。 本发明还涉及从外消旋醛和氰化物制备氰醇和/或羧酸，优选光学活性的氰醇和/或羧酸的方法。

    Modified nitrilases and their use in methods for the production of carboxylic acids
    7.
    发明专利
    Modified nitrilases and their use in methods for the production of carboxylic acids 未知

    公开(公告)号：AU2004215238A1

    公开(公告)日：2004-09-10

    申请号：AU2004215238

    申请日：2004-02-24

    Applicant: BASF AG

    Inventor： ZELINSKI THOMAS ,  KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS

    IPC: C12N9/78 ,  C12N15/55 ,  C12N15/82 ,  C12P7/40

    Abstract: The present Invention relates to new nitrilases with modified Substrate acceptance, the method to obtain them and the use of the aforesaid nitrilases. Said nitrilases are encoded by a polypeptide sequence, which at a Position corresponding to Position 296 in wild-type Alcaligenes faecalis comprises an amino acid which is not tyrosine. The Invention further relates to nucleic acid sequences and amino acid sequences encoding Said nitrilases, expression constructs comprising Said nucleic acid sequences, vectors comprising the nucleic acid sequences or the exPression constructs, to organisms, preferably microorganisms, comprising the nucleic acid sequences, expression constructs or vectors. The Invention additionally relates to a method for preparing carboxylic acids, preferably substituted chiral carboxylic acids from racemic nitriles.

    New nucleic acid encoding L-pantolactone hydrolase, useful for producing pure D-pantolactone, an intermediate for vitamins, from its racemate
    10.
    发明专利
    New nucleic acid encoding L-pantolactone hydrolase, useful for producing pure D-pantolactone, an intermediate for vitamins, from its racemate 未知

    公开(公告)号：DE19952501A1

    公开(公告)日：2001-05-03

    申请号：DE19952501

    申请日：1999-10-29

    Applicant: BASF AG

    Inventor： KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  MATTES RALF

    IPC: C12N9/18 ,  C12N15/55 ,  C12P17/04 ,  C12P41/00

    Abstract: Isolated nucleic acid (I), encoding a polypeptide with L-pantolactone hydrolase (LPH) activity comprising a 810 base pair sequence (1), equivalents of (1) within the degeneracy of the genetic code, is new. All sequences are fully defined in the specification. Independent claims are also included for the following: (a) derivatives of (1) that encode a polypeptide with at least 50% homology with a 269 amino acid sequence (2), without significant loss of enzymatic activity; (b) polypeptides (II) encoded by (I); (c) nucleic acid constructs containing (I) linked to one or more regulatory elements; (d) vector containing (I) or the construct of (b); (e) microorganism containing (I), the construct of (b) or the vector of (c); (f) LPH (IIa) with specified properties; and (g) production of D-pantolactone (III) by treating racemic pantolactone with (IIa) or the microorganisms of (d). All sequences are fully defined in the specification.

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