公开(公告)号：PT1123386E

公开(公告)日：2007-03-30

申请号：PT99952558

申请日：1999-10-13

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  FRIEDRICH THOMAS ,  RESS-LOESCHKE MARION ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N9/78 ,  C07H21/00 ,  C12N15/09 ,  C12N1/21 ,  C12N15/55 ,  C12N15/63 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05

公开(公告)号：DE59914102D1

公开(公告)日：2007-02-08

申请号：DE59914102

申请日：1999-10-13

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N9/78 ,  C12N15/09 ,  C07H21/00 ,  C12N1/21 ,  C12N15/55 ,  C12N15/63 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.

公开(公告)号：PL359496A1

公开(公告)日：2004-08-23

申请号：PL35949601

申请日：2001-02-27

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19 ,  A61P41/00

Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).

公开(公告)号：ZA200207902B

公开(公告)日：2004-03-29

申请号：ZA200207902

申请日：2002-10-02

Applicant: BASF AG

Inventor： ENGELS DIRK ,  HAUER BERNHARD ,  MATTES RALF ,  RESS-LOESCHKE MARION

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19 ,  C12N ,  C12P

Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).

公开(公告)号：NO20024169L

公开(公告)日：2002-11-01

申请号：NO20024169

申请日：2002-09-02

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19 ,  C12N

Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).

公开(公告)号：NO20024169A

公开(公告)日：2002-11-01

申请号：NO20024169

申请日：2002-09-02

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19 ,  C12N

CPC classification number: C12P41/006 ,  C12N9/78