公开(公告)号：CA2347521C

公开(公告)日：2010-02-23

申请号：CA2347521

申请日：1999-10-13

Applicant: BASF AG

Inventor： RESS-LOSCHKE MARION ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N9/78 ,  C12N15/09 ,  C07H21/00 ,  C12N1/21 ,  C12N15/55 ,  C12N15/63 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide with nitrilase activity, to nucleic acid constructs containing the nucleic acid sequences, and to vectors containing the nucleic acid sequences or the nucleic acid constructs. The invention also relates to amino acid sequences which are coded by the nucleic acid sequences, and to microorganisms containing the nucleic acid sequences, the nucleic acid constructs or vectors containing the nucleic acid sequences or the nucleic acid constructs. In addition, the invention relates to a method for producing chiral carboxylic acids from the racemic nitriles.

公开(公告)号：NO327857B1

公开(公告)日：2009-10-05

申请号：NO20011912

申请日：2001-04-18

Applicant: BASF AG

Inventor： MATTES RALF ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  RESS-LOSCHKE MARION ,  ENGELS DIRK

IPC: C07H21/00 ,  C12N9/78 ,  C12N1/21 ,  C12N15/09 ,  C12N15/55 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.

公开(公告)号：EE05008B1

公开(公告)日：2008-04-15

申请号：EEP200100232

申请日：1999-10-13

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N9/78 ,  C12N15/09 ,  C07H21/00 ,  C12N1/21 ,  C12N15/55 ,  C12N15/63 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.

公开(公告)号：DK1123386T3

公开(公告)日：2007-05-07

申请号：DK99952558

申请日：1999-10-13

Applicant: BASF AG

Inventor： MATTES RALF ,  HAUER BERNHARD ,  RESS-LOESCHKE MARION ,  FRIEDRICH THOMAS ,  ENGELS DIRK

IPC: C12N9/78 ,  C12N15/09 ,  C07H21/00 ,  C12N1/21 ,  C12N15/55 ,  C12N15/63 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.

公开(公告)号：BR0108883A

公开(公告)日：2003-04-29

申请号：BR0108883

申请日：2001-02-27

Applicant: BASF AG

Inventor： RESS-LDSCHKE MARION ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19 ,  C12N15/70 ,  C12N15/76

Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).

公开(公告)号：AU3380201A

公开(公告)日：2001-09-12

申请号：AU3380201

申请日：2001-02-27

Applicant: BASF AG

Inventor： RESS-LOSCHKE MARION ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19 ,  C12N15/70 ,  C12N15/76

Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).

公开(公告)号：HU0300155A2

公开(公告)日：2003-05-28

申请号：HU0300155

申请日：2001-02-27

Applicant: BASF AG

Inventor： ENGELS DIRK ,  HAUER BERNHARD ,  MATTES RALF ,  RESS-LOESCHKE MARION

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19

Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).

公开(公告)号：EE200100232A

公开(公告)日：2002-08-15

申请号：EEP200100232

申请日：1999-10-13

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N15/09 ,  C07H21/00 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05 ,  C12N15/63

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.

公开(公告)号：ZA200104066B

公开(公告)日：2002-07-01

申请号：ZA200104066

申请日：2001-05-18

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C07H21/00 ,  C12N1/21 ,  C12N9/78 ,  C12N15/09 ,  C12N15/55 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05 ,  C12N ,  C12P ,  C12R

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.

公开(公告)号：CA2400446A1

公开(公告)日：2001-09-07

申请号：CA2400446

申请日：2001-02-27

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  RESS-LOSCHKE MARION ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19 ,  C12N15/70 ,  C12N15/76

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide with nitrilase activity, nucleic acid constructs containing said nucleic aci ds and vectors containing said nucleic acids or said nucleic acid constructs. T he invention also relates to amino acid sequences which are coded by the nuclei c acid sequences and micro-organisms containing said nucleic acid sequences, said nucleic acid constructs or vectors containing said nucleic acid sequenc es or said nucleic acid constructs. The invention also relates to an enzymatic method for producing carboxylic acids from the corresponding nitriles.