公开(公告)号：BRPI0017148B1

公开(公告)日：2016-03-01

申请号：BR0017148

申请日：2000-12-22

Applicant: BASF AKTIENGESELLSCHFAT ,  BASF SE ,  EVONIK DEGUSSA GMBH

Inventor： KRÖGER BURKHARD ,  HWANG BYUNG-JOON ,  HABERHAUER GREGOR ,  SCHRÖDER HARTWIG ,  LEE HEUNG-SHICK ,  KIM JUN-WON ,  POMPEJUS MARKUS ,  ZELDER OSKAR

IPC: C07K14/34 ,  C12N15/09 ,  C07K20060101 ,  C12N20060101 ,  C12N1/21 ,  C12N9/90 ,  C12N15/54 ,  C12P20060101 ,  C12P13/08 ,  C12P13/12 ,  C12P21/02 ,  C12R1/13 ,  C12R1/15

Abstract: "molécula de ácido nucleico isolada, vetor, célula hospedeira, método para produzir um polipeptídeo, polipeptídeo de caminho metabólico, polipeptídeo isolado, e, métodos para produzir um produto químico fino e para modular o rendimento de um produto químico fino a partir de uma célula". moléculas de ácido nucleico isoladas, designadas moléculas de ácido nucleico mp, que codificam novas proteínas mp de corynebacterium glutamicum são descritas. a invenção também fornece moléculas de ácido nucleico de anti-sentido, vetores de expressão recombinantes contendo moléculas de ácido nucleico mp e células hospedeiras nas quais os vetores de expressão foram introduzidos. a invenção ainda fornece proteínas mp isoladas, proteínas mp mutadas, proteínas de fusão, peptídeos antigênicos e métodos para a melhoria da produção de um composto desejado a partir do c. glutamicum com base no engendramento genético de genes mp neste organismo.

公开(公告)号：ZA200805545B

公开(公告)日：2014-03-26

申请号：ZA200805545

申请日：2008-06-25

Applicant: BASF SE

Inventor： POMPEJUS MARKUS ,  FREYER STEPHAN ,  LOHSCHEIDT MARKUS ,  ZELDER OSKAR ,  BOY MATTHIAS

IPC: C12P20060101

Abstract: Preparation of at least one organic compound (I) with at least 3 C atoms or at least two C and at least one N by fermentation. Preparation of at least one organic compound (I) with at least 3 C atoms or at least two C and at least one N by fermentation comprises: (a) millling a starch source (S) to form a milled product (MP) that contains at least part of the non-starch-containing solid components (X) of (S); (b) suspending (MP) in an aqueous liquid and hydrolysis of the starch components by enzymatic liquefaction and optionally subsequent saccharification, to form a first liquid medium (1), containing mono- or oligo-saccharides; and (c) adding (1), together with metabolizable mono-, di- and/or oligo-saccharides (Y), or compositions containing at least 50wt.% (Y) essentially free of water-insoluble solids, to a fermentation medium that contains microorganisms (MO) which can overproduce (I), under fermentation conditions.

公开(公告)号：CA2875603A1

公开(公告)日：2014-01-09

申请号：CA2875603

申请日：2013-07-03

Applicant: WINTERSHALL HOLDING GMBH ,  BASF SE

Inventor： BROCKMANN BEATA ,  HEROLD ANDREA ,  ZELDER OSKAR ,  HAEFNER STEFAN ,  FLECK CHRISTIAN ,  SCHRODER HARTWIG ,  GRANSTROM MARI ,  SCHMIDT JULIA KRISTIANE

IPC: C12N9/10 ,  C12P19/04

Abstract: The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that said genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-ß-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-ß-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-ß-D-glucan synthase-activity or the use of such a polypeptide for producing ß-glucans. Furthermore, the present invention relates to methods for producing ß-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-ß-D-glucan synthase-activity thereby increasing the expression of said polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-ß-D-glucan synthase-activity into a microorganism being able to synthesize ß-glucans.

公开(公告)号：BRPI0615697A2

公开(公告)日：2012-12-18

申请号：BRPI0615697

申请日：2006-09-06

Applicant: BASF SE

Inventor： POMPEJUS MARKUS ,  FREYER STEPHAN ,  LOHSCHEIDT MARKUS ,  BOY MATTHIAS ,  SCHOLTEN EDZARD ,  ZELDER OSKAR

IPC: C12P19/14 ,  A23L7/104 ,  A23L35/00 ,  C12P13/08 ,  C12P13/14

Abstract: The present invention relates to a method for the production of at least one nonvolatile microbial metabolite in solid form by sugar-based microbial fermentation, in which process a microorganism strain which produces the desired metabolites is grown using a sugar-containing liquid medium with a monosaccharide content of more than 20% by weight based on the total weight of the liquid medium, and the volatile constituents of the fermentation liquor are subsequently largely removed, the sugar-containing liquid medium being prepared by: a1) milling selected starch feedstock from cereal grains; and a2) liquefying the millbase in an aqueous liquid in the presence of at least one starch-liquefying enzyme, followed by saccharification using at least one saccharifying enzyme, where, for liquefaction purposes, at least a portion of the millbase is liquefied by continuous or batchwise addition to the aqueous liquid. Furthermore, the invention relates to a solid formulation, of a nonvolatile microbial metabolite, obtainable by the method according to the invention; and to the use of such a solid formulation as additive or supplement for human or animal nutrition or for the treatment of textiles, leather, cellulose, paper or surfaces.

公开(公告)号：ES2380000T3

公开(公告)日：2012-05-07

申请号：ES07123772

申请日：2005-12-21

Applicant: BASF SE

Inventor： HAEFNER STEFAN ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  HEROLD ANDREA

IPC: C12N15/77 ,  C07K14/34

Abstract: Bacteria corineforme genéticamente modificada, transformada con al menos un vector que contiene al menos un casete de expresión, en cuyo caso el casete de expresión comprende en dirección 5' -3' un módulo de secuencia de la siguiente fórmula general II: 5' -P1-(-Ax-Px-)n-Ay-Py-G-3' (II) Donde n representa un valor de número entero de 0 a 10, Ax y Ay son iguales o diferentes y representan un enlace químico o una secuencia de ácido nucleico enlazante; P1, Px y Py codifican secuencias de promotores iguales o diferentes derivadas de bacterias corineformes iguales o diferentes, que comprenden al menos un segmento que enlaza polimerasa de ARN; y al menos Py comprende un segmento de secuencia terminal 3' que facilita el enlace ribosómico, G representa al menos una secuencia de ácido nucleico codificante que está enlazada funcionalmente con la secuencia regulatoria ubicada corriente arriba de 5'.

公开(公告)号：PL1942198T3

公开(公告)日：2012-04-30

申请号：PL07123761

申请日：2005-07-16

Applicant: BASF SE

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  KRÖGER BURKHARD ,  SCHRÖDER HARTWIG ,  HAEFNER STEFAN

IPC: C12Q1/68 ,  C07K14/34

公开(公告)号：AT531819T

公开(公告)日：2011-11-15

申请号：AT07123761

申请日：2005-07-16

Applicant: BASF SE

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: C12Q1/68 ,  C07K14/34

Abstract: A specific nucleic acid (I) with promoter activity is used for transcribing genes, where (I) is: (a) a sequence (1) of 178 nucleotides (reproduced); (b) a derivative of (1) with >= 90% identity, formed by substitution, insertion or deletion; (c) a sequence that hybridizes to (1) under stringent conditions; or (d) a functional fragment of (a)-(c). Independent claims are also included for: (1) use of an expression unit (EU), containing and linked to a nucleic acid (NA) that ensures translation of RNA, for expressing genes; (2) (I), except sequence (1) itself, as new compounds and EU containing it; (3) method for altering (or causing) the transcription (or expression) rate of genes in microorganisms relative to the wild type; (4) expression cassette (EC) comprising EU, at least one other functionally linked NA to be expressed and optionally other genetic control elements that are heterologous with respect to EU; (5) expression vector containing EC; (6) genetically modified microorganism (GMM) in which the transcription rate of at least one gene is altered (or caused) relative to the wild type; (7) GMM containing EU and a functionally linked gene to be expressed, where this is heterologous with respect to EU; (8) preparation of biosynthetic products (A) by culturing the GMM of (6) or (7); (9) use of the sequence aggagga (21) as ribosome-binding site in expression units for expressing genes in Corynebacterium or Brevibacterium; (10) use of the sequences ttaatt (19) or taagct (20) as -10 regions in EU for expressing genes in Corynebacterium or Brevibacterium; and (11) EU that contain (21) or at least one of (19) or (20).

公开(公告)号：BRPI0709628A2

公开(公告)日：2011-07-19

申请号：BRPI0709628

申请日：2007-03-23

Applicant: BASF SE

Inventor： ZELDER OSKAR ,  JEONG WEOL KYU ,  KLOPPROGGE CORINNA ,  HEROLD ANDREA ,  SCHROEDER HARTWIG

IPC: C12P13/00 ,  C12P13/02 ,  C12R1/15

Abstract: Process for the production of cadaverine by constructing a recombinant microorganism which has a deregulated lysine decarboxylase gene and at least one deregulated gene selected from the group (i) which consists of aspartokinase, aspartatesemialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate succinylase, succinyl-amino-ketopimelate transaminase, succinyl-diamino-pimelate desuccinylase, diaminopimelate epimerase, diaminopimelate dehydrogenase, arginyl-tRNA synthetase, diaminopimelate decarboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxylase, glucose-6-phosphate dehydrogenase, transketolase, transaldolase, 6-phosphogluconolactonase, fructose 1,6-biphosphatase, homoserine dehydrogenase, phophoenolpyruvate carboxykinase, succinyl-CoA synthetase, methylmalonyl-CoA mutase, provided that if aspartokinase is deregulated as gene (i) at least a second gene (i) other than aspartokinase has to be deregulated, and cultivating said microorganism.

公开(公告)号：CA2712427A1

公开(公告)日：2009-08-13

申请号：CA2712427

申请日：2009-02-04

Applicant: BASF SE

Inventor： ZELDER OSKAR ,  JEONG WEOL KYU ,  KLOPPROGGE CORINNA ,  HEROLD ANDREA ,  SCHROEDER HARTWIG

IPC: C12P17/12 ,  C12N9/02

Abstract: The present invention relates to a novel method for the fermentative production of dipicolinate by cultivating a recombinant microorganism expressing an enzyme having dipicolinate synthetase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as a method of preparing polyester or polyamide copolymers making use of dipicolinate as obtained by fermentative production.

公开(公告)号：AU2009211870A1

公开(公告)日：2009-08-13

申请号：AU2009211870

申请日：2009-02-04

Applicant: BASF SE

Inventor： JEONG WEOL KYU ,  HEROLD ANDREA ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHRODER HARTWIG

IPC: C12P17/12 ,  C12N9/02

Abstract: The present invention relates to a novel method for the fermentative production of dipicolinate by cultivating a recombinant microorganism expressing an enzyme having dipicolinate synthetase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as a method of preparing polyester or polyamide copolymers making use of dipicolinate as obtained by fermentative production.