公开(公告)号：DE4123874A1

公开(公告)日：1992-01-23

申请号：DE4123874

申请日：1991-07-18

Applicant: BIO RAD LABORATORIES

Inventor： FERNWOOD GEORGE G ,  PENALUNA WILLIAM A ,  GEISSELSODER JANET

IPC: B01D35/02 ,  B01D29/01 ,  B01D61/18 ,  B01D63/08 ,  B01L3/00 ,  C12M1/12

Abstract: A plate assembly for performing multiple filtrations with the use of a vacuum offers advantages for small samples due to the construction of its component plates, which include an upper plate with apertures forming sample wells, a membrane sheet forming the floor of each well, a collection plate with individual wells underneath the membrane sheet, aligned with the upper plate apertures, and a drop guide plate between the membrane sheet and the collection plate to guide and force small quantities of liquid downward from the membrane into the collection wells. The arrangement permits extremely small quantities of species on the membrane to be drawn into the collection wells without dilution, and avoids cross contamination between collection wells.

公开(公告)号：ITRM910512A1

公开(公告)日：1992-01-19

申请号：ITRM910512

申请日：1991-07-11

Applicant: BIO RAD LABORATORIES

Inventor： FERNWOOD GEORGE G ,  GEISSELSODER JANET ,  PENALUNA WILLIAM A

IPC: B01D35/02 ,  B01D20060101 ,  B01D29/01 ,  B01D61/18 ,  B01D63/08 ,  B01L3/00 ,  C12M1/12

公开(公告)号：GB2184238B

公开(公告)日：1989-12-28

申请号：GB8619267

申请日：1986-08-07

Applicant: BIO RAD LABORATORIES

Inventor： SLEETER DONALD D ,  FERNWOOD GEORGE G ,  BURD SAMUEL

IPC: C08F2/10 ,  G01N27/26 ,  G01N27/447

Abstract: A gel used for electrophoresis is cast in a gel enclosure in which the gel will ultimately be used during the electrophoresis, by a novel method and using novel apparatus which provide for quick assembly and avoidance of leaks. The method and apparatus involve a wettable, preferably porous, membrane such as filter paper, which is thoroughly wetted with gel forming solution, then placed over the bottom opening of the gel enclosure and held in place until the gel on the membrane sets. Sealing of any cracks, gaps or irregularities along the rim of the opening is thereby achieved by the capillary action of the gel forming solution. The enclosure itself is then filled with gel forming solution. Once the latter has set, the membrane is removed.

公开(公告)号：GB2181248B

公开(公告)日：1989-11-15

申请号：GB8615341

申请日：1986-06-24

Applicant: BIO RAD LABORATORIES

Inventor： SLEETER DONALD DAVID ,  VON ALVEN RAYMOND DOUGLAS ,  FERNWOOD GEORGE G

IPC: G01N33/50 ,  G01N27/26 ,  G01N27/447

Abstract: An integrated plate structure provides a novel component for use in an electrophoretic sequencing cell. The integrated plate structure consists of a glass plate and a plastic plate bonded together along a raised edge of the latter, the edge extending along the sides and bottom of the plastic plate. The plates thus form a permanent enclosure for an upper buffer solution, open at the top for filling. The glass plate further serves as one of the two glass plates forming the gel enclosure.