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公开(公告)号：DE04024743T1
公开(公告)日：2005-10-20
申请号：DE04024743
申请日：1995-06-13
Applicant: BIO RAD LABORATORIES
Inventor： RAGSDALE CHARLES W
Abstract: A method for operating a transfection system adapted to deliver a prespecified high-voltage current to a chemical solution, comprising the steps of: asserting a trigger signal when power is removed from the transfection system; triggering a crowbar circuit coupled to a charge storage device that provides the high-voltage current when said trigger signal is asserted; and discharging said charge storage device through said crowbar circuit when said crowbar circuit is activated. An apparatus for directing a first predetermined current profile at a second predetermined high voltage level to a chemical solution is also disclosed, along with a hi-joule switch and a capacitor expansion module.
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公开(公告)号：CA2437062A1
公开(公告)日：2003-06-19
申请号：CA2437062
申请日：2002-12-06
Applicant: BIO RAD LABORATORIES
Inventor： RAGSDALE CHARLES W
Abstract: Cuvette inserts adapted and configured to fit within an electroporation cuvette. The inserts each include a support structure that holds a porous membrane. When positioned within the cuvette, the membrane is positioned proximal the cuvette electrodes to facilitate membrane-based fusion of cells . In certain aspects, a tube extends through the support structure to allow fo r application of negative pressure in a convenient location away from electrod e contacts and other components of the cuvette or cuvette holder.
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公开(公告)号：CA1326507C
公开(公告)日：1994-01-25
申请号：CA538821
申请日：1987-06-04
Applicant: BIO RAD LABORATORIES
Inventor： RAGSDALE CHARLES W
IPC: H02M9/04 , C12M1/00 , C12M1/42 , C12M3/00 , C12N13/00 , C12N15/00 , C12N15/02 , C12N15/87 , H02M1/088 , H02M9/02 , H03K3/57 , H03K17/10 , A61N1/00 , A61N1/18 , B01J19/08
Abstract: 23 TRANSFECTION HIGH-VOLTAGE CONTROLLER A transfection high-voltage controller for directing high-voltage currents to a suspension of cells and DNA comprising a control section, a power store, and a high joule switch. Under control of the control section, the high joule switch directs a high-voltage current from the power store to a load such as a cuvette. The high-joule switch comprises a semiconductor controlled rectifier (SCR) having a gate coupled to the control unit for triggering the device, an anode for receiving the high-voltage current from the power store, and a cathode for delivering the high-voltage current to the cuvette. A current sink may be connected to the anode of the SCR to render it non-conductive a predetermined time after the high-voltage current appears at the cathode. Multiple SCRs may be connected in series to support voltages over 3,000 and currents in excess of 125 amperes, and a trigger control unit may be attached to the gate of each SCR in the series to effect simultaneous or serial triggering of the group.
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Patent Agency Ranking
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公开(公告)号：CA2670933C
公开(公告)日：2013-05-21
申请号：CA2670933
申请日：2007-11-26
Applicant: BIO RAD LABORATORIES
Inventor： RAGSDALE CHARLES W
Abstract: Systems, methods and apparatus provide flexible and efficient high throughput electroporation systems. An electrical pulse may be transmitted to any number of channels of a multi-channel sample plate. Drivers can provide the selection of which channels to transmit the electrical pulse. To provide efficient transitions between electrical pulses, discharge circuits provide efficient means achieve a desired voltage.
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28.发明专利
公开(公告)号：AU2002353072B2
公开(公告)日：2007-04-05
申请号：AU2002353072
申请日：2002-12-06
Applicant: BIO RAD LABORATORIES
Inventor： CHIU TONY , RAGSDALE CHARLES W
Abstract: Systems, methods and apparatus for stabilizing resistance measurements in electroporation systems so as to achieve high-accuracy measurements, and for controlling the pulse duration, or time constant, of electroporation pulses. Circuitry is provided to accurately determine the resistance of a sample in the electroporation system, for example a sample provided in an electroporation cuvette. Additionally, a resistance control system is provided to automatically measure the resistance of a sample, determine a capacitance and determine a parallel add-on resistance that substantially provides a desired pulse duration (time constant) for an electroporation pulse.
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公开(公告)号：AU2002366605B2
公开(公告)日：2005-10-20
申请号：AU2002366605
申请日：2002-12-06
Applicant: BIO RAD LABORATORIES
Inventor： RAGSDALE CHARLES W
IPC: C12M1/00 , C12M1/42 , C12M3/00 , C12M001/42
Abstract: Cuvette inserts adapted and configured to fit within an electroporation cuvette. The inserts each include a support structure that holds a porous membrane. When positioned within the cuvette, the membrane is positioned proximal the cuvette electrodes to facilitate membrane-based fusion of cells. In certain aspects, a tube extends through the support structure to allow for application of negative pressure in a convenient location away from electrode contacts and other components of the cuvette or cuvette holder.
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公开(公告)号：CA2523040A1
公开(公告)日：2004-12-09
申请号：CA2523040
申请日：2004-05-20
Applicant: BIO RAD LABORATORIES
Inventor： RAGSDALE CHARLES W , CHU DANIEL Y , ARCINIEGAS GERMAN , CEREMONY JEFF
Abstract: Individual temperature control in multiple reactions performed simultaneousl y in a spatial array such as a multi-well plate is achieved by thermoelectric modules with individual control, with each module supplying heat to or drawi ng heat from a single region within the array, the region containing either a single reaction vessel or a group of reaction vessels.
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