公开(公告)号：ES2357115T3

公开(公告)日：2011-04-18

申请号：ES04765718

申请日：2004-09-30

Applicant: BASF SE

Inventor： STUERMER RAINER ,  KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  BREUER MICHAEL

IPC: C07D333/16 ,  C07D333/20 ,  C12N9/04 ,  C12N15/53 ,  C12N15/63 ,  C12P7/04

Abstract: Método para la producción de (S)-3-metilamino-1-(tien-2-il)-propan-1-ol de la fórmula I-S en el cual a) reacciona tiofeno con un halogenuro de ß-halopropionilo o un halogenuro de acriloilo en presencia de un ácido Lewis para dar una 3-halo-1-(tien-2-il)-propan-1-ona, donde se introduce un haluro de hidrógeno simultáneamente o después de terminada la reacción, aunque antes del aislamiento del producto de reacción, y b) se reduce la propanona obtenida en la etapa a) en presencia de una deshidrogenasa (E.C. 1.1.x.x.), la cual exhibe una selectividad respecto a la formación de (S)-3-metilamino-1-(tien-2-il)propan-1-ol, y a continuación, dado el caso, se hace reaccionar con metilamina sin aislamiento del producto de reacción.

公开(公告)号：DK1929001T3

公开(公告)日：2010-08-09

申请号：DK06806768

申请日：2006-09-14

Applicant: BASF SE

Inventor： BREUER MICHAEL ,  FRIEDRICH THOMAS ,  KESSELER MARIA

IPC: C12N9/04

公开(公告)号：AT466075T

公开(公告)日：2010-05-15

申请号：AT06806768

申请日：2006-09-14

Applicant: BASF SE

Inventor： BREUER MICHAEL ,  FRIEDRICH THOMAS ,  KESSELER MARIA

IPC: C12N9/04

Abstract: The present invention relates to proteins having an enzymatic activity of reducing substituted alkanones such as 3-methylamino-1-(2-thienyl)-propan-1-one. The invention furthermore relates to nucleic acids coding for said proteins, nucleic acid constructs, vectors, genetically modified microorganisms and to methods for preparing optically active substituted alkanols, such as, for example, (S)-3-methylamino-1-(2-thienyl)-(S)-propanol.

公开(公告)号：AT435278T

公开(公告)日：2009-07-15

申请号：AT04713882

申请日：2004-02-24

Applicant: BASF SE

Inventor： ZELINSKI THOMAS ,  KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS

IPC: C12N9/78 ,  C12N15/55 ,  C12N15/82 ,  C12P7/40

Abstract: The present Invention relates to new nitrilases with modified Substrate acceptance, the method to obtain them and the use of the aforesaid nitrilases. Said nitrilases are encoded by a polypeptide sequence, which at a Position corresponding to Position 296 in wild-type Alcaligenes faecalis comprises an amino acid which is not tyrosine. The Invention further relates to nucleic acid sequences and amino acid sequences encoding Said nitrilases, expression constructs comprising Said nucleic acid sequences, vectors comprising the nucleic acid sequences or the exPression constructs, to organisms, preferably microorganisms, comprising the nucleic acid sequences, expression constructs or vectors. The Invention additionally relates to a method for preparing carboxylic acids, preferably substituted chiral carboxylic acids from racemic nitriles.

公开(公告)号：DE502004006579D1

公开(公告)日：2008-04-30

申请号：DE502004006579

申请日：2004-04-06

Applicant: BASF SE

Inventor： ALTHOEFER HENNING ,  KESSELER MARIA

IPC: C12N15/53 ,  C12N9/04 ,  C12P7/04 ,  C12P13/00 ,  C12P17/00 ,  C12P41/00

Abstract: The present invention relates to proteins having an enzymatic activity of reducing substituted alkanones such as 3-methylamino-1-(2-thienyl)-propan-1-one. The invention furthermore relates to nucleic acids coding for said proteins, nucleic acid constructs, vectors, genetically modified microorganisms and to methods for preparing substituted (S)-alkanols, such as, for example, (S)-3-methylamino-1-(2-thienyl)-(S)-propanol.