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公开(公告)号：WO2019186322A1
公开(公告)日：2019-10-03
申请号：PCT/IB2019/052229
申请日：2019-03-19
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： BABA, Takashi
Abstract: A mass isolation device selects a precursor ion of a sample that has been digested using a protease. A first fragmentation device fragments the precursor ion using collision-induced dissociation (CID), and the resulting product ions are analyzed using a mass analyzer producing a CID spectrum. A list of theoretical candidate glycopeptide sequences is determined from CID spectrum. The mass isolation device again selects the precursor ion of the sample. A second fragmentation device fragments the precursor ion using electron-based dissociation (ExD), and the resulting product ions are analyzed using the mass analyzer producing a CID spectrum. For each sequence of the list, the sequence is computationally fragmented, producing theoretical fragments, mass-to-charge ratio (m/z) values are calculated for the theoretical fragments, and the sequence is scored using c and z fragment matching rules. The highest scoring sequence is identified as a peptide sequence of a glycopeptide of the sample.
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公开(公告)号：WO2019058226A1
公开(公告)日：2019-03-28
申请号：PCT/IB2018/057017
申请日：2018-09-13
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： BABA, Takashi , DZIEKONSKI, Eric Thomas
Abstract: One or more ions are received along a central axis through a first set of reflectron plates of an ELIT. Voltages are applied to the first set of plates and to a second set of reflectron plates in order to trap and oscillate the one or more ions. A first induced current is measured from a cylindrical pickup electrode between the first set of reflectron plates and the second set of reflectron plates. A second induced current is measured from one or more plates of the first set of reflectron plates. A third induced current is measured from one or more plates of the second set of reflectron plates. The first measured induced current, second measured induced current and third measured induced current are combined to reduce higher order frequency harmonics of the induced current.
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公开(公告)号：WO2017103860A1
公开(公告)日：2017-06-22
申请号：PCT/IB2016/057691
申请日：2016-12-15
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： BABA, Takashi , BAKER, Paul , CAMPBELL, John L. , LEBLANC, Yves
CPC classification number: H01J49/0054 , G01N33/92
Abstract: A method and apparatus for analyzing samples using mass spectrometry are disclosed. The apparatus includes a reaction device configured to dissociate sample ions into fragments by reacting the sample ions with a charged species (e.g., electrons) such as through ECD, EID, or EIEIO. The kinetic energy of the charged species is such that the fragments may be detected and produce spectra that allow for the determination of isomeric species in the sample and the location of double bonds and/or the orientation of those double bonds within the sample molecules. The fragments may include radical fragments and non-radical fragments. Spectra resulting from analysis of the fragments may allow for the determination of the oxygen-radical fragments resulting from the dissociation of the sample molecules as confirmation of the presence of those radical fragments.
Abstract translation: 公开了使用质谱法分析样品的方法和设备。 该装置包括反应装置，该反应装置被配置为通过使样品离子与带电物质（例如电子）例如通过ECD，EID或EIE10反应而将样品离子离解成片段。 带电物质的动能使得可以检测碎片并产生允许样品中异构物质的确定和样品分子内双键的位置和/或那些双键的取向的光谱。 片段可以包括自由基片段和非自由基片段。 通过分析片段得到的光谱可以允许确定样品分子解离产生的氧自由基片段作为确认这些自由基片段的存在。
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公开(公告)号：WO2016181299A1
公开(公告)日：2016-11-17
申请号：PCT/IB2016/052658
申请日：2016-05-10
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： BABA, Takashi
CPC classification number: G01N33/6848 , G01N2560/00 , H01J49/0054
Abstract: Systems and methods described herein can provide for "top down" mass spectrometric analysis of proteins or peptides in a sample using ExD, in some aspects via direct infusion of the sample to the ion source without on-line LC separation, while deconvoluting the ambiguity in the ExD spectra generated by impure samples. For example, methods and systems in accordance with various aspects of the present teachings can utilize patterns in charge-reduced species following ExD to correlate the ExD fragments with their precursor ions in order to more confidently identify the precursor ion from which the detected product ions originated.
Abstract translation: 本文所述的系统和方法可以提供使用ExD的样品中蛋白质或肽的“自上而下”质谱分析，在某些方面，通过将样品直接输入到离子源而不进行在线LC分离，同时解卷积歧义 不纯样品产生的ExD光谱。 例如，根据本教导的各个方面的方法和系统可以利用ExD之后的电荷减少的物质中的模式来将ExD片段与其前体离子相关联，以便更加自信地鉴定起源于检测到的产物离子的前体离子 。
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公开(公告)号：WO2016108142A1
公开(公告)日：2016-07-07
申请号：PCT/IB2015/059856
申请日：2015-12-21
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： BABA, Takashi
IPC: H01J49/40
CPC classification number: H01J49/0072 , H01J49/0054 , H01J49/062 , H01J49/063
Abstract: A method and apparatus for conducting reactions between precursor ions and reagent ions, for example, a reaction between a precursor cation and an electron, such as ECD, are disclosed. The apparatus comprises first, second, and third pathways, each of which extends at least partially along a central axis, and wherein the second central axis is orthogonal to the first and third central axes. Charged species can be introduced into the second pathway as the ions are transmitted therethrough, thereby increasing precursor ion and charged species interaction without simultaneous trapping of the species.
Abstract translation: 公开了用于进行前体离子和试剂离子之间的反应的方法和装置，例如前体阳离子和电子之间的反应，例如ECD。 该装置包括第一，第二和第三通道，每个通道至少部分地沿着中心轴线延伸，并且其中第二中心轴线与第一和第三中心轴线正交。 当离子通过其中时，带电物质可以被引入到第二通路中，由此增加前体离子和带电物质的相互作用而不同时捕获物种。
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Patent Agency Ranking
公开(公告)号：WO2015101816A1
公开(公告)日：2015-07-09
申请号：PCT/IB2014/002495
申请日：2014-11-18
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： BABA, Takashi
IPC: H01J49/26
CPC classification number: H01J49/062 , H01J49/06
Abstract: Methods and systems for transmitting ions in an ion guide are provided herein. In accordance with various aspects of the applicant's teachings, the methods and systems can cause at least a portion of ions entrained in a gas flow entering an ion guide to be extracted from the gas jet and be guided downstream along one or more path of gas flow, where the gas lacking the ions can be removed from the ion guide. In some embodiments, the ions extracted from the gas stream can be guided into a focusing region in which the ions can be focused, e.g., via RF focusing, to enter into subsequence processing stages, such as a mass analyzer.
Abstract translation: 本文提供了用于在离子导向器中传输离子的方法和系统。 根据申请人的教导的各个方面，所述方法和系统可以使夹带在进入离子导向器的气流中的至少一部分离子从气体射流中提取出来，并沿气流的一个或多个路径向下游引导 ，其中可以从离子导向器去除缺乏离子的气体。 在一些实施方案中，从气流提取的离子可以被引导到聚焦区域中，其中离子可以例如通过RF聚焦被聚焦，以进入子序列处理阶段，例如质量分析器。
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公开(公告)号：WO2013098614A1
公开(公告)日：2013-07-04
申请号：PCT/IB2012/002621
申请日：2012-12-06
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： BABA, Takashi
CPC classification number: H01J49/06 , H01J49/26 , H01J49/4225 , H01J49/4255
Abstract: A method is provided for processing ions in a multipole ion trap, comprising generating RF radial confinement fields within a first and second multipole rod set positioned in tandem, a ratio of q value exhibited by the second rod set relative to the first rod set being greater than one for any m/z, said RF axial confinement fields within the first and second rod sets interacting in an interaction region between the first and second rod sets so as to produce a fringing field; transmitting ions through said first rod set towards said second rod set; and increasing the radial oscillation amplitude of at least a portion of the ions within said first rod set such that at least a portion of said ions having an increased radial oscillation amplitude are repulsed by said fringing field.
Abstract translation: 提供了一种用于处理多极离子阱中的离子的方法，包括在串联定位的第一和第二多极棒组内产生RF径向限制场，第二棒组相对于第一棒组显示的q值的比值更大 所述第一和第二杆组内的所述RF轴向限制场在所述第一和第二杆组之间的相互作用区域中相互作用以产生边缘场; 将离子通过所述第一杆组传递到所述第二杆组; 以及增加所述第一棒组中的离子的至少一部分的径向振荡振幅，使得具有增加的径向振荡幅度的所述离子的至少一部分被所述边缘场排斥。
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公开(公告)号：WO2022013716A1
公开(公告)日：2022-01-20
申请号：PCT/IB2021/056258
申请日：2021-07-12
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： BABA, Takashi , KARASAWA, Kaoru
Abstract: In one aspect, a method of performing mass spectrometry is disclosed, which comprises ionizing a plurality of oligonucleotides to generate a plurality of negatively charged oligonucleotide ions, and interacting a plurality of charged reagent ions with the negatively charged oligonucleotide ions to reduce the negative charge state of the negatively charged oligonucleotide ions.
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公开(公告)号：WO2022009095A1
公开(公告)日：2022-01-13
申请号：PCT/IB2021/056054
申请日：2021-07-06
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： LIU, Suya , BABA, Takashi
Abstract: A separation time of an isomer of one or more isomers of a sialylated glycopeptide of a sample is calculated from a peak of a precursor XIC. Product ion intensities of the first group are summed at the separation time producing a first sum and product ion intensities of the second group are summed at the separation time producing a second sum using XICs of the first and second groups. A ratio of the first sum to the second sum is calculated. The ratio at the separation time is compared to predetermined ratio ranges that each corresponds to a combination of a selection from a set of the first linkage and the second linkage taken one or more times. One or more linkages of the sialic acid to the glycan of the isomer are identified from a combination found to match the ratio in the comparison.
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公开(公告)号：WO2020044160A1
公开(公告)日：2020-03-05
申请号：PCT/IB2019/056936
申请日：2019-08-15
Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Inventor： BABA, Takashi , RYUMIN, Pavel , LOYD, William M.
Abstract: A dissociation device fragments a precursor ion, producing at least two different product ions with overlapping m/z values in the dissociation device. The dissociation device applies an AC voltage and a DC voltage creating a pseudopotential that traps ions below a threshold m/z including the at least two product ions. The dissociation device receives a charge reducing reagent that causes the trapped at least two product ions to be charge reduced until their m/z values increase above the threshold m/z set by the AC voltage. The increase in the m/z values of the at least two product ions decreases their overlap. The at least two product ions with increased m/z values are transmitted to another device for subsequent mass analysis by applying the DC voltage to the dissociation device relative to a DC voltage applied to the other device.
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